Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,576 T→C 8.7% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5760TC 8.7% 263.7 / 12.7 102intergenic (+21/+220)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base T (54/34);  new base C (4/4);  total (59/43)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-02

Only 100 of 103 total aligned reads displayed.

ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAA  >  NC_000913/857427‑857722
                                                                                                                                                     |                                                                                                                                                   
aTCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAt                                                                                                                                                     <  1:748248/150‑1 (MQ=255)
  cTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATAc                                                                                                                                                   >  2:514788/1‑150 (MQ=255)
    ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                 >  2:1521249/1‑150 (MQ=255)
    ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                 >  1:833804/1‑150 (MQ=255)
         tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                           <  1:490509/150‑1 (MQ=255)
         tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACACCGATACTGTCggg                                                                                                                                           >  1:1446402/1‑150 (MQ=255)
         tCACACGACCGTGAGTTCGTAAGCTCCCTGCCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                           <  1:1147628/150‑1 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGCGt                                                                                                                                         >  1:1225083/1‑150 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                         <  2:651159/150‑1 (MQ=255)
           acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGAGCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                         >  1:1148607/1‑150 (MQ=255)
            cacGACCGTGAGTGAGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTg                                                                                                                                        <  1:697470/150‑1 (MQ=255)
             acGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGa                                                                                                                                       >  1:1402325/1‑150 (MQ=255)
               gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAcg                                                                                                                                     <  1:1619522/150‑1 (MQ=255)
                    tGATTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGTATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                                <  2:801888/150‑1 (MQ=255)
                          cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTATAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGA                                                                                                                          >  1:1039732‑M1/1‑150 (MQ=255)
                          cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                         >  2:474778/1‑135 (MQ=255)
                           gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                         >  1:1449106‑M1/1‑150 (MQ=255)
                           gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                         >  2:938440‑M1/1‑150 (MQ=255)
                            tAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACA                                                                                                                        >  2:940744‑M1/1‑150 (MQ=255)
                               gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAT                                                                                                                     >  1:1640540‑M1/1‑150 (MQ=255)
                                cTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATG                                                                                                                    <  2:1002413‑M1/150‑1 (MQ=255)
                                  cccTGGCGCCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACATGCGAAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCACGATAATGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                  >  1:989530/1‑150 (MQ=255)
                                   ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCTTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAataaa                                                                                                                 >  1:301401‑M1/1‑145 (MQ=255)
                                    cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCtaa                                                                                                                >  1:995438‑M1/1‑147 (MQ=255)
                                           cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                         >  2:184136/1‑150 (MQ=255)
                                               cATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                                     >  2:779207/1‑150 (MQ=255)
                                                aTTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgc                                                                                                    <  1:224374/150‑1 (MQ=255)
                                                     ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                >  2:209698/1‑150 (MQ=255)
                                                     ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGAGCTAc                                                                                               >  1:306015/1‑150 (MQ=255)
                                                     ggAAATCACCCCGGAACGCGTGATCGACTTGAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTAc                                                                                               >  1:688646/1‑150 (MQ=255)
                                                            aCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATc                                                                                        <  1:38898/150‑1 (MQ=255)
                                                                cGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGc                                                                                    <  2:1047053/150‑1 (MQ=255)
                                                                      gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                              <  1:47508/150‑1 (MQ=255)
                                                                       cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTTCGCa                                                                             >  1:209853/1‑150 (MQ=255)
                                                                          gATCGACTTTCGCGGTAATTCCTAATATTACGTGCGTAGTACTGGGAGCGAGTAAGTGATTATCCGTCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTa                                                                          <  1:574530/150‑1 (MQ=37)
                                                                             cGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCACTACGCTTATCAGGCCTACGCAGTACCt                                                                       >  1:496878/1‑150 (MQ=255)
                                                                              gACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTg                                                                      >  1:501317/1‑150 (MQ=255)
                                                                               aCTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                     >  1:1230607/1‑150 (MQ=255)
                                                                               aCTTAAGCGTTAATAACGATTATTACCTGCGTAGTAAAGGGATGTATTAAGTGTTTATCCGTCACCCCGATACTTTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                     <  1:1389080/150‑1 (MQ=37)
                                                                                       ggTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                             >  1:334396/1‑150 (MQ=255)
                                                                                       ggTAATTACGAAGATTACATGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                             <  2:739400/150‑1 (MQ=255)
                                                                                          aaTTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGaa                                                          >  2:188524/1‑150 (MQ=255)
                                                                                              aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTg                                                      >  1:1370026/1‑150 (MQ=255)
                                                                                               cGAACATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGGCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                     <  1:729114/150‑1 (MQ=255)
                                                                                                gAAGATTACAGGCGTAGTACAGTGATCCAGTCAGTGTATATCCGTCACCCCTAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCATATCAGGCCTACTCAGTACCTGCAATATATTGAATCTGCa                                                    <  1:274960/150‑1 (MQ=255)
                                                                                                 aaGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCAGATCCTCTACGCTTATCAGGCCTACGCATTACCTGCAATATATTGAATCTGCat                                                   >  1:129369/1‑149 (MQ=255)
                                                                                                  tttttACCTGGGTTTAAACGGCATCGCGTATGTTATTAGCGGCCACCCCAAAACTTGGGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                  <  1:1343999/147‑1 (MQ=18)
                                                                                                    tttACCTGCGTAGTATAGGGATCGTGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:102725/149‑1 (MQ=255)
                                                                                                    ataacttGCGTAGTAATGGGATCGAGTATGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:818143/144‑1 (MQ=255)
                                                                                                    aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:1208291/150‑1 (MQ=255)
                                                                                                    aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                >  2:463213/1‑150 (MQ=255)
                                                                                                       aCCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACttt                                             <  2:833804/150‑1 (MQ=255)
                                                                                                         cTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGt                                           >  2:905395/1‑150 (MQ=255)
                                                                                                          tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                          >  2:277242/1‑150 (MQ=255)
                                                                                                            cGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcctgcagattcaatatattgcaggt                                        >  1:241784‑M1/1‑75 (MQ=255)
                                                                                                             gtagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTTAATCTGCAGGACTTTGTAGGc                                       >  2:311163/1‑150 (MQ=255)
                                                                                                              tagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGcc                                      >  2:346886/1‑150 (MQ=255)
                                                                                                               tgtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                     <  2:1112904/149‑1 (MQ=255)
                                                                                                               agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                     >  1:1330777/1‑150 (MQ=255)
                                                                                                                ttaAAGGTATCTAGTAAGGTTTTATCCGTAACCCCGATACTGTGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                    <  2:1394431/149‑1 (MQ=25)
                                                                                                                  aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                  >  1:88306/1‑150 (MQ=255)
                                                                                                                   aaGGGATCGAGTAAGTGATTATCCGTCACCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCTGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                 >  1:1303543/1‑150 (MQ=255)
                                                                                                                     gggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                               >  1:1530281/1‑150 (MQ=255)
                                                                                                                      ggATCGAGTAAGTGATTATCGGTCACCGCGATAGTGTCGGGGTGACGGCGGCTGATGACAAATGAAAAATAGCCTGATGCGCTACGCTTATCAGGCCTAGGCAGTACCTGCAATATTTTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                              <  2:920717/150‑1 (MQ=37)
                                                                                                                       gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                             >  2:1256851/1‑150 (MQ=255)
                                                                                                                       gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                             >  1:408117/1‑150 (MQ=255)
                                                                                                                        agcGAGTAAGTGATTATCCGTCACCCCGAAACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGTGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                            <  1:851605/148‑1 (MQ=255)
                                                                                                                        aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                            >  1:1594113/1‑150 (MQ=255)
                                                                                                                         tCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                           <  2:1225083/150‑1 (MQ=255)
                                                                                                                              tAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcg                      <  2:1640540/150‑1 (MQ=255)
                                                                                                                                aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                    >  2:1730793/1‑150 (MQ=255)
                                                                                                                                aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                    >  2:1131566/1‑150 (MQ=255)
                                                                                                                                 ttGATAATCCTTCACCCCGATCCTGTCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                   <  2:877224/149‑1 (MQ=255)
                                                                                                                                 gggATTATCCGACCCCCCGATACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                   <  1:1521249/148‑1 (MQ=255)
                                                                                                                                  tGATTATCCGTCTCCCCGATACTGTCTGGGGGACGGCGGCTGATGACAAATTCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                  <  2:301401/150‑1 (MQ=255)
                                                                                                                                   gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                 >  1:791994/1‑150 (MQ=255)
                                                                                                                                    aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCAt                <  2:1730944/150‑1 (MQ=255)
                                                                                                                                    aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGATTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCCGGACTTTGTAGGCCGGATAAGTCGTTAACGACGCaa                >  2:1044908/1‑149 (MQ=38)
                                                                                                                                     ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc               <  1:724798/150‑1 (MQ=255)
                                                                                                                                     gccgccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATTTCCAGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc               >  2:1645115/5‑150 (MQ=21)
                                                                                                                                      aatcccgcACCCCTAAACTGCCGGGGGTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa              <  1:1349786/143‑1 (MQ=21)
                                                                                                                                       aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATTTTGATTCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg             >  1:976201/1‑150 (MQ=255)
                                                                                                                                        tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg            >  1:972641/1‑150 (MQ=255)
                                                                                                                                         cggccACCCCTATCCTGCGGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc           <  2:1536468/146‑1 (MQ=21)
                                                                                                                                         ccGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc           <  2:1682119/150‑1 (MQ=255)
                                                                                                                                          cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa          >  1:1091774/1‑150 (MQ=255)
                                                                                                                                          cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa          >  1:355011/1‑150 (MQ=255)
                                                                                                                                           gTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCAGCATCAGGCAt         >  2:1085094/1‑150 (MQ=255)
                                                                                                                                            tCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt         <  1:209698/150‑1 (MQ=255)
                                                                                                                                            cccccccGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCTGATGCGGCGTTAACGCCTTATCCGGccta        <  1:514788/147‑4 (MQ=21)
                                                                                                                                             cACCCCGATACTGTTGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       <  2:892552/150‑1 (MQ=255)
                                                                                                                                             cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:123837/1‑150 (MQ=255)
                                                                                                                                             cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:1003967/1‑150 (MQ=255)
                                                                                                                                             cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:365530/1‑150 (MQ=255)
                                                                                                                                              aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                               >  2:1496879‑M1/1‑41 (MQ=255)
                                                                                                                                              aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                               <  1:1496879‑M1/93‑53 (MQ=255)
                                                                                                                                               ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc     >  2:1450411/1‑150 (MQ=255)
                                                                                                                                               ccccGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc     <  1:856487/150‑1 (MQ=255)
                                                                                                                                                cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCAACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa    >  2:1097079/1‑150 (MQ=255)
                                                                                                                                                  cGATACTGTCGGGGTGACGGCGGCAGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaaa  <  1:232199/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAA  >  NC_000913/857427‑857722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: