Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,578 C→G 8.6% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5780CG 8.6% 289.9 / 14.0 101intergenic (+23/+218)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base C (53/38);  new base G (4/5);  total (58/43)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 102 total aligned reads displayed.

CTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAA  >  NC_000913/857429‑857722
                                                                                                                                                     |                                                                                                                                                 
cTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATAc                                                                                                                                                   >  2:514788/1‑150 (MQ=255)
  ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                 >  2:1521249/1‑150 (MQ=255)
  ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                 >  1:833804/1‑150 (MQ=255)
       tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                           <  1:490509/150‑1 (MQ=255)
       tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACACCGATACTGTCggg                                                                                                                                           >  1:1446402/1‑150 (MQ=255)
       tCACACGACCGTGAGTTCGTAAGCTCCCTGCCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                           <  1:1147628/150‑1 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGCGt                                                                                                                                         >  1:1225083/1‑150 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                         <  2:651159/150‑1 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGAGCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                         >  1:1148607/1‑150 (MQ=255)
          cacGACCGTGAGTGAGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTg                                                                                                                                        <  1:697470/150‑1 (MQ=255)
           acGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGa                                                                                                                                       >  1:1402325/1‑150 (MQ=255)
             gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAcg                                                                                                                                     <  1:1619522/150‑1 (MQ=255)
                  tGATTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGTATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                                <  2:801888/150‑1 (MQ=255)
                        cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTATAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGA                                                                                                                          >  1:1039732‑M1/1‑150 (MQ=255)
                        cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                         >  2:474778/1‑135 (MQ=255)
                        cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                         <  1:474778/135‑1 (MQ=255)
                         gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                         >  1:1449106‑M1/1‑150 (MQ=255)
                         gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                         >  2:938440‑M1/1‑150 (MQ=255)
                          tAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACA                                                                                                                        >  2:940744‑M1/1‑150 (MQ=255)
                             gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAT                                                                                                                     >  1:1640540‑M1/1‑150 (MQ=255)
                              cTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATG                                                                                                                    <  2:1002413‑M1/150‑1 (MQ=255)
                                cccTGGCGCCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACATGCGAAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCACGATAATGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                  >  1:989530/1‑150 (MQ=255)
                                 ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCTTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAataaa                                                                                                                 >  1:301401‑M1/1‑145 (MQ=255)
                                  cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCtaa                                                                                                                >  1:995438‑M1/1‑147 (MQ=255)
                                         cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                         >  2:184136/1‑150 (MQ=255)
                                             cATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                                     >  2:779207/1‑150 (MQ=255)
                                              aTTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgc                                                                                                    <  1:224374/150‑1 (MQ=255)
                                                   ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                >  2:209698/1‑150 (MQ=255)
                                                   ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGAGCTAc                                                                                               >  1:306015/1‑150 (MQ=255)
                                                   ggAAATCACCCCGGAACGCGTGATCGACTTGAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTAc                                                                                               >  1:688646/1‑150 (MQ=255)
                                                          aCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATc                                                                                        <  1:38898/150‑1 (MQ=255)
                                                              cGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGc                                                                                    <  2:1047053/150‑1 (MQ=255)
                                                                    gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                              <  1:47508/150‑1 (MQ=255)
                                                                     cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTTCGCa                                                                             >  1:209853/1‑150 (MQ=255)
                                                                        gATCGACTTTCGCGGTAATTCCTAATATTACGTGCGTAGTACTGGGAGCGAGTAAGTGATTATCCGTCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTa                                                                          <  1:574530/150‑1 (MQ=37)
                                                                           cGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCACTACGCTTATCAGGCCTACGCAGTACCt                                                                       >  1:496878/1‑150 (MQ=255)
                                                                            gACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTg                                                                      >  1:501317/1‑150 (MQ=255)
                                                                             aCTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                     >  1:1230607/1‑150 (MQ=255)
                                                                             aCTTAAGCGTTAATAACGATTATTACCTGCGTAGTAAAGGGATGTATTAAGTGTTTATCCGTCACCCCGATACTTTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                     <  1:1389080/150‑1 (MQ=37)
                                                                                     ggTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                             >  1:334396/1‑150 (MQ=255)
                                                                                     ggTAATTACGAAGATTACATGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                             <  2:739400/150‑1 (MQ=255)
                                                                                        aaTTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGaa                                                          >  2:188524/1‑150 (MQ=255)
                                                                                            aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTg                                                      >  1:1370026/1‑150 (MQ=255)
                                                                                            aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGAAATATATTGATTCTg                                                      >  2:1349786/1‑150 (MQ=255)
                                                                                             cGAACATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGGCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                     <  1:729114/150‑1 (MQ=255)
                                                                                              gAAGATTACAGGCGTAGTACAGTGATCCAGTCAGTGTATATCCGTCACCCCTAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCATATCAGGCCTACTCAGTACCTGCAATATATTGAATCTGCa                                                    <  1:274960/150‑1 (MQ=255)
                                                                                               aaGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCAGATCCTCTACGCTTATCAGGCCTACGCATTACCTGCAATATATTGAATCTGCat                                                   >  1:129369/1‑149 (MQ=255)
                                                                                                tttttACCTGGGTTTAAACGGCATCGCGTATGTTATTAGCGGCCACCCCAAAACTTGGGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                  <  1:1343999/147‑1 (MQ=18)
                                                                                                  tttACCTGCGTAGTATAGGGATCGTGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:102725/149‑1 (MQ=255)
                                                                                                  ataacttGCGTAGTAATGGGATCGAGTATGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:818143/144‑1 (MQ=255)
                                                                                                  aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                <  2:1208291/150‑1 (MQ=255)
                                                                                                  aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                >  2:463213/1‑150 (MQ=255)
                                                                                                     aCCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACttt                                             <  2:833804/150‑1 (MQ=255)
                                                                                                       cTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGt                                           >  2:905395/1‑150 (MQ=255)
                                                                                                        tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                          >  2:277242/1‑150 (MQ=255)
                                                                                                          cGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcctgcagattcaatatattgcaggt                                        >  1:241784‑M1/1‑75 (MQ=255)
                                                                                                           gtagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTTAATCTGCAGGACTTTGTAGGc                                       >  2:311163/1‑150 (MQ=255)
                                                                                                            tagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGcc                                      >  2:346886/1‑150 (MQ=255)
                                                                                                             tgtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                     <  2:1112904/149‑1 (MQ=255)
                                                                                                             agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                     >  1:1330777/1‑150 (MQ=255)
                                                                                                              ttaAAGGTATCTAGTAAGGTTTTATCCGTAACCCCGATACTGTGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                    <  2:1394431/149‑1 (MQ=25)
                                                                                                                aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                  >  1:88306/1‑150 (MQ=255)
                                                                                                                 aaGGGATCGAGTAAGTGATTATCCGTCACCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCTGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                 >  1:1303543/1‑150 (MQ=255)
                                                                                                                   gggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                               >  1:1530281/1‑150 (MQ=255)
                                                                                                                    ggATCGAGTAAGTGATTATCGGTCACCGCGATAGTGTCGGGGTGACGGCGGCTGATGACAAATGAAAAATAGCCTGATGCGCTACGCTTATCAGGCCTAGGCAGTACCTGCAATATTTTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                              <  2:920717/150‑1 (MQ=37)
                                                                                                                     gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                             >  2:1256851/1‑150 (MQ=255)
                                                                                                                     gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                             >  1:408117/1‑150 (MQ=255)
                                                                                                                      agcGAGTAAGTGATTATCCGTCACCCCGAAACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGTGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                            <  1:851605/148‑1 (MQ=255)
                                                                                                                      aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                            >  1:1594113/1‑150 (MQ=255)
                                                                                                                       tCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                           <  2:1225083/150‑1 (MQ=255)
                                                                                                                            tAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcg                      <  2:1640540/150‑1 (MQ=255)
                                                                                                                              aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                    >  2:1131566/1‑150 (MQ=255)
                                                                                                                              aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                    >  2:1730793/1‑150 (MQ=255)
                                                                                                                               ttGATAATCCTTCACCCCGATCCTGTCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                   <  2:877224/149‑1 (MQ=255)
                                                                                                                               gggATTATCCGACCCCCCGATACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                   <  1:1521249/148‑1 (MQ=255)
                                                                                                                                tGATTATCCGTCTCCCCGATACTGTCTGGGGGACGGCGGCTGATGACAAATTCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                  <  2:301401/150‑1 (MQ=255)
                                                                                                                                 gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                 >  1:791994/1‑150 (MQ=255)
                                                                                                                                  aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCAt                <  2:1730944/150‑1 (MQ=255)
                                                                                                                                  aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGATTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCCGGACTTTGTAGGCCGGATAAGTCGTTAACGACGCaa                >  2:1044908/1‑149 (MQ=38)
                                                                                                                                   ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc               <  1:724798/150‑1 (MQ=255)
                                                                                                                                   gccgccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATTTCCAGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc               >  2:1645115/5‑150 (MQ=21)
                                                                                                                                    aatcccgcACCCCTAAACTGCCGGGGGTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa              <  1:1349786/143‑1 (MQ=21)
                                                                                                                                     aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATTTTGATTCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg             >  1:976201/1‑150 (MQ=255)
                                                                                                                                       cggccACCCCTATCCTGCGGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc           <  2:1536468/146‑1 (MQ=21)
                                                                                                                                       ccGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc           <  2:1682119/150‑1 (MQ=255)
                                                                                                                                        cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa          >  1:1091774/1‑150 (MQ=255)
                                                                                                                                        cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa          >  1:355011/1‑150 (MQ=255)
                                                                                                                                         gTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCAGCATCAGGCAt         >  2:1085094/1‑150 (MQ=255)
                                                                                                                                          tCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt         <  1:209698/150‑1 (MQ=255)
                                                                                                                                          cccccccGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCTGATGCGGCGTTAACGCCTTATCCGGccta        <  1:514788/147‑4 (MQ=21)
                                                                                                                                           cACCCCGATACTGTTGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       <  2:892552/150‑1 (MQ=255)
                                                                                                                                           cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:123837/1‑150 (MQ=255)
                                                                                                                                           cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:1003967/1‑150 (MQ=255)
                                                                                                                                           cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa       >  2:365530/1‑150 (MQ=255)
                                                                                                                                            aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                               >  2:1496879‑M1/1‑41 (MQ=255)
                                                                                                                                            aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                               <  1:1496879‑M1/93‑53 (MQ=255)
                                                                                                                                             ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc     >  2:1450411/1‑150 (MQ=255)
                                                                                                                                             ccccGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc     <  1:856487/150‑1 (MQ=255)
                                                                                                                                              cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCAACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa    >  2:1097079/1‑150 (MQ=255)
                                                                                                                                                cGATACTGTCGGGGTGACGGCGGCAGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaaa  <  1:232199/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAA  >  NC_000913/857429‑857722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: