Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,580 G→A 8.1% intergenic (+25/+216) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5800GA 8.1% 287.8 / 9.7 99intergenic (+25/+216)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base G (52/37);  new base A (4/4);  total (56/43)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

Only 100 of 102 total aligned reads displayed.

TTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGCATT  >  NC_000913/857431‑857729
                                                                                                                                                     |                                                                                                                                                      
ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                        >  2:1521249/1‑150 (MQ=255)
ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                        >  1:833804/1‑150 (MQ=255)
     tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                                  <  1:490509/150‑1 (MQ=255)
     tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACACCGATACTGTCggg                                                                                                                                                  >  1:1446402/1‑150 (MQ=255)
     tCACACGACCGTGAGTTCGTAAGCTCCCTGCCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                                  <  1:1147628/150‑1 (MQ=255)
       acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGCGt                                                                                                                                                >  1:1225083/1‑150 (MQ=255)
       acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                                <  2:651159/150‑1 (MQ=255)
       acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGAGCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                                >  1:1148607/1‑150 (MQ=255)
        cacGACCGTGAGTGAGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTg                                                                                                                                               <  1:697470/150‑1 (MQ=255)
         acGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGa                                                                                                                                              >  1:1402325/1‑150 (MQ=255)
           gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAcg                                                                                                                                            <  1:1619522/150‑1 (MQ=255)
                tGATTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGTATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                                       <  2:801888/150‑1 (MQ=255)
                      cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTATAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGA                                                                                                                                 >  1:1039732‑M1/1‑150 (MQ=255)
                      cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                                >  2:474778/1‑135 (MQ=255)
                      cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGt                                                                                                                                                <  1:474778/135‑1 (MQ=255)
                       gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                                >  1:1449106‑M1/1‑150 (MQ=255)
                       gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAC                                                                                                                                >  2:938440‑M1/1‑150 (MQ=255)
                        tAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACA                                                                                                                               >  2:940744‑M1/1‑150 (MQ=255)
                           gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAT                                                                                                                            >  1:1640540‑M1/1‑150 (MQ=255)
                            cTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATG                                                                                                                           <  2:1002413‑M1/150‑1 (MQ=255)
                              cccTGGCGCCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACATGCGAAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCACGATAATGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                         >  1:989530/1‑150 (MQ=255)
                               ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCTTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAataaa                                                                                                                        >  1:301401‑M1/1‑145 (MQ=255)
                                cTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCtaa                                                                                                                       >  1:995438‑M1/1‑147 (MQ=255)
                                       cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                                >  2:184136/1‑150 (MQ=255)
                                           cATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                                            >  2:779207/1‑150 (MQ=255)
                                            aTTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgc                                                                                                           <  1:224374/150‑1 (MQ=255)
                                                 ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                       >  2:209698/1‑150 (MQ=255)
                                                 ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGAGCTAc                                                                                                      >  1:306015/1‑150 (MQ=255)
                                                 ggAAATCACCCCGGAACGCGTGATCGACTTGAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTAc                                                                                                      >  1:688646/1‑150 (MQ=255)
                                                        aCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATc                                                                                               <  1:38898/150‑1 (MQ=255)
                                                            cGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGc                                                                                           <  2:1047053/150‑1 (MQ=255)
                                                                  gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                                     <  1:47508/150‑1 (MQ=255)
                                                                   cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTTCGCa                                                                                    >  1:209853/1‑150 (MQ=255)
                                                                      gATCGACTTTCGCGGTAATTCCTAATATTACGTGCGTAGTACTGGGAGCGAGTAAGTGATTATCCGTCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTa                                                                                 <  1:574530/150‑1 (MQ=37)
                                                                         cGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCACTACGCTTATCAGGCCTACGCAGTACCt                                                                              >  1:496878/1‑150 (MQ=255)
                                                                          gACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTg                                                                             >  1:501317/1‑150 (MQ=255)
                                                                           aCTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                            >  1:1230607/1‑150 (MQ=255)
                                                                           aCTTAAGCGTTAATAACGATTATTACCTGCGTAGTAAAGGGATGTATTAAGTGTTTATCCGTCACCCCGATACTTTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                            <  1:1389080/150‑1 (MQ=37)
                                                                                   ggTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                                    >  1:334396/1‑150 (MQ=255)
                                                                                   ggTAATTACGAAGATTACATGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                                    <  2:739400/150‑1 (MQ=255)
                                                                                      aaTTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGaa                                                                 >  2:188524/1‑150 (MQ=255)
                                                                                          aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTg                                                             >  1:1370026/1‑150 (MQ=255)
                                                                                          aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGAAATATATTGATTCTg                                                             >  2:1349786/1‑150 (MQ=255)
                                                                                           cGAACATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGGCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                            <  1:729114/150‑1 (MQ=255)
                                                                                            gAAGATTACAGGCGTAGTACAGTGATCCAGTCAGTGTATATCCGTCACCCCTAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCATATCAGGCCTACTCAGTACCTGCAATATATTGAATCTGCa                                                           <  1:274960/150‑1 (MQ=255)
                                                                                             aaGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCAGATCCTCTACGCTTATCAGGCCTACGCATTACCTGCAATATATTGAATCTGCat                                                          >  1:129369/1‑149 (MQ=255)
                                                                                              tttttACCTGGGTTTAAACGGCATCGCGTATGTTATTAGCGGCCACCCCAAAACTTGGGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                         <  1:1343999/147‑1 (MQ=18)
                                                                                                tttACCTGCGTAGTATAGGGATCGTGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                       <  2:102725/149‑1 (MQ=255)
                                                                                                ataacttGCGTAGTAATGGGATCGAGTATGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                       <  2:818143/144‑1 (MQ=255)
                                                                                                aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                       <  2:1208291/150‑1 (MQ=255)
                                                                                                aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                       >  2:463213/1‑150 (MQ=255)
                                                                                                   aCCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACttt                                                    <  2:833804/150‑1 (MQ=255)
                                                                                                     cTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGt                                                  >  2:905395/1‑150 (MQ=255)
                                                                                                      tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                                 >  2:277242/1‑150 (MQ=255)
                                                                                                        cGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcctgcagattcaatatattgcaggt                                               >  1:241784‑M1/1‑75 (MQ=255)
                                                                                                         gtagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTTAATCTGCAGGACTTTGTAGGc                                              >  2:311163/1‑150 (MQ=255)
                                                                                                          tagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGcc                                             >  2:346886/1‑150 (MQ=255)
                                                                                                           tgtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                            <  2:1112904/149‑1 (MQ=255)
                                                                                                           agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                            >  1:1330777/1‑150 (MQ=255)
                                                                                                            ttaAAGGTATCTAGTAAGGTTTTATCCGTAACCCCGATACTGTGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                           <  2:1394431/149‑1 (MQ=25)
                                                                                                              aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                         >  1:88306/1‑150 (MQ=255)
                                                                                                               aaGGGATCGAGTAAGTGATTATCCGTCACCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCTGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                        >  1:1303543/1‑150 (MQ=255)
                                                                                                                 gggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                                      >  1:1530281/1‑150 (MQ=255)
                                                                                                                  ggATCGAGTAAGTGATTATCGGTCACCGCGATAGTGTCGGGGTGACGGCGGCTGATGACAAATGAAAAATAGCCTGATGCGCTACGCTTATCAGGCCTAGGCAGTACCTGCAATATTTTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                                     <  2:920717/150‑1 (MQ=37)
                                                                                                                   gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                                    >  1:408117/1‑150 (MQ=255)
                                                                                                                   gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGc                                    >  2:1256851/1‑150 (MQ=255)
                                                                                                                    agcGAGTAAGTGATTATCCGTCACCCCGAAACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGTGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                                   <  1:851605/148‑1 (MQ=255)
                                                                                                                    aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                                   >  1:1594113/1‑150 (MQ=255)
                                                                                                                     tCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                                  <  2:1225083/150‑1 (MQ=255)
                                                                                                                          tAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcg                             <  2:1640540/150‑1 (MQ=255)
                                                                                                                            aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                           >  2:1131566/1‑150 (MQ=255)
                                                                                                                            aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                           >  2:1730793/1‑150 (MQ=255)
                                                                                                                             ttGATAATCCTTCACCCCGATCCTGTCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                          <  2:877224/149‑1 (MQ=255)
                                                                                                                             gggATTATCCGACCCCCCGATACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                          <  1:1521249/148‑1 (MQ=255)
                                                                                                                              tGATTATCCGTCTCCCCGATACTGTCTGGGGGACGGCGGCTGATGACAAATTCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                         <  2:301401/150‑1 (MQ=255)
                                                                                                                               gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                        >  1:791994/1‑150 (MQ=255)
                                                                                                                                aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCAt                       <  2:1730944/150‑1 (MQ=255)
                                                                                                                                aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGATTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCCGGACTTTGTAGGCCGGATAAGTCGTTAACGACGCaa                       >  2:1044908/1‑149 (MQ=38)
                                                                                                                                 ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc                      <  1:724798/150‑1 (MQ=255)
                                                                                                                                 gccgccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATTTCCAGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc                      >  2:1645115/5‑150 (MQ=21)
                                                                                                                                  aatcccgcACCCCTAAACTGCCGGGGGTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCAGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa                     <  1:1349786/143‑1 (MQ=21)
                                                                                                                                   aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATTTTGATTCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg                    >  1:976201/1‑150 (MQ=255)
                                                                                                                                     cggccACCCCTATCCTGCGGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc                  <  2:1536468/146‑1 (MQ=21)
                                                                                                                                     ccGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc                  <  2:1682119/150‑1 (MQ=255)
                                                                                                                                      cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa                 >  1:1091774/1‑150 (MQ=255)
                                                                                                                                      cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa                 >  1:355011/1‑150 (MQ=255)
                                                                                                                                       gTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCAGCATCAGGCAt                >  2:1085094/1‑150 (MQ=255)
                                                                                                                                        tCACCCCGATACTGTCGGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt                <  1:209698/150‑1 (MQ=255)
                                                                                                                                        cccccccGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCTGATGCGGCGTTAACGCCTTATCCGGccta               <  1:514788/147‑4 (MQ=21)
                                                                                                                                         cACCCCGATACTGTTGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa              <  2:892552/150‑1 (MQ=255)
                                                                                                                                         cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa              >  2:123837/1‑150 (MQ=255)
                                                                                                                                         cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa              >  2:1003967/1‑150 (MQ=255)
                                                                                                                                         cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa              >  2:365530/1‑150 (MQ=255)
                                                                                                                                          aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                                      >  2:1496879‑M1/1‑41 (MQ=255)
                                                                                                                                          aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCgctttgttaatgcctgatgcggcgttaacgccttatccggcctacaaagtcc                                                                      <  1:1496879‑M1/93‑53 (MQ=255)
                                                                                                                                           ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc            >  2:1450411/1‑150 (MQ=255)
                                                                                                                                           ccccGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc            <  1:856487/150‑1 (MQ=255)
                                                                                                                                            cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCAACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa           >  2:1097079/1‑150 (MQ=255)
                                                                                                                                              cGATACTGTCGGGGTGACGGCGGCAGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaaa         <  1:232199/150‑1 (MQ=255)
                                                                                                                                                     gTCGGGGTGACGGCGGCTTATGACAAATTCAAAATAGCCTGATGCGCTACGCTTATCAGTCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCgcatt  >  2:306015‑M2/1‑145 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGCATT  >  NC_000913/857431‑857729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: