Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,020 G→T 6.5% L18L (CTG→CTT rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,0200GT 6.5% 318.5 / 7.4 106L18L (CTG→CTTrimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (46/53);  new base T (2/5);  total (48/58)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 106 total aligned reads displayed.

ATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCG  >  NC_000913/891872‑892168
                                                                                                                                                    |                                                                                                                                                    
   attTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCTTGTAAGCGGCTGCGTg                                                                                                                                                  >  2:1703231/1‑150 (MQ=255)
   attTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTg                                                                                                                                                  >  2:1645427/1‑150 (MQ=255)
        cacaAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGcc                                                                                                                                             >  2:800178/1‑150 (MQ=255)
           aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                          <  2:373627/150‑1 (MQ=255)
           aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                          <  2:868692/150‑1 (MQ=255)
           aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                          <  2:601037/150‑1 (MQ=255)
           aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGGCGATGTATGATACGCGGGCGTTTGACCAGGTCTGACAGAGAGGGGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCt                                                                                                                                          <  1:840525/150‑1 (MQ=255)
           aaaCAGGGGTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                          <  2:539773/150‑1 (MQ=255)
            aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGACCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                         >  2:881755/1‑150 (MQ=255)
            aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                         >  2:797221/1‑150 (MQ=255)
            aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                         >  2:1025966/1‑150 (MQ=255)
             aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                        <  2:217384/150‑3 (MQ=255)
             aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                        <  1:1676115/150‑3 (MQ=255)
             aCAGGGTTGGGCGACGCGCTAAAACCGCCACGGCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                        <  1:544757/150‑3 (MQ=255)
                tggTTGGGCGACGCGCTAAATGCGCCACGTCTATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCTTATTGTCCCGGGATGTAACGCTATATTCGTGTAATCGGCTTCACGCAGCCGCttacac                                                                                                                                     <  2:1491594/149‑6 (MQ=255)
                gggTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACa                                                                                                                                     >  1:1351715/1‑150 (MQ=255)
                  gTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgc                                                                                                                                   <  1:1528822/150‑1 (MQ=255)
                  gTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgc                                                                                                                                   >  1:840571/1‑150 (MQ=255)
                    tGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgcgc                                                                                                                                 >  1:1687578/1‑150 (MQ=255)
                    tGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgcgc                                                                                                                                 <  1:11241/150‑1 (MQ=255)
                    ggggCGACGCGCGAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgcgc                                                                                                                                 <  1:1451737/149‑1 (MQ=255)
                      ggCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCgg                                                                                                                               <  2:490607/150‑1 (MQ=255)
                       gCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGCCAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGt                                                                                                                              >  2:862334/1‑150 (MQ=255)
                           cgcgCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCAcc                                                                                                                          >  1:511893/1‑150 (MQ=255)
                            gcgcTAAACCCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCt                                                                                                                         <  1:1071491/150‑1 (MQ=255)
                            gcgcTAAAACCGCCACGGCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGGGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCt                                                                                                                         <  1:482684/150‑1 (MQ=255)
                             cgcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                        >  1:838584/1‑150 (MQ=255)
                             cgcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                        >  1:989379/1‑150 (MQ=255)
                              gcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCAGCTATACAGCGCGGTCACCTgg                                                                                                                       >  2:1480317/1‑150 (MQ=255)
                               cTAACACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGCACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                      <  1:1634124/150‑1 (MQ=255)
                               cTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGAGGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                      <  1:1627768/150‑1 (MQ=255)
                                tAAAACCGCCACGTCGAGGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGtt                                                                                                                     <  1:632403/150‑1 (MQ=255)
                                 aaaaCCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTg                                                                                                                    >  1:887051/1‑150 (MQ=255)
                                     ccGCCACGTCGAGGTATGATACGCGGGCTTTTGTCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATAGAGCGGGGTCACCTGGTTGAAAt                                                                                                                <  1:241727/150‑1 (MQ=255)
                                        ccACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCTTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                             <  2:557541/150‑1 (MQ=255)
                                        ccACGGCGCTGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                             <  1:186806/150‑1 (MQ=255)
                                         cACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCtt                                                                                                            >  2:1035591/1‑150 (MQ=255)
                                             tCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATc                                                                                                        <  1:702371/150‑1 (MQ=255)
                                                  gTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCtt                                                                                                   >  1:1008714/1‑150 (MQ=255)
                                                   tATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCttt                                                                                                  >  2:1103186/1‑150 (MQ=255)
                                                      gATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCtt                                                                                               <  1:975937/150‑1 (MQ=255)
                                                         aCGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCt                                                                                            <  1:950129/150‑1 (MQ=255)
                                                          cgcgGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTa                                                                                           <  1:1026216/150‑1 (MQ=255)
                                                           gcgGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGGCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTAc                                                                                          <  2:80310/150‑1 (MQ=255)
                                                             gggCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACAt                                                                                        <  1:1487909/150‑1 (MQ=255)
                                                               gCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGa                                                                                      <  1:1103186/150‑1 (MQ=255)
                                                                cTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                      >  1:1357974/1‑117 (MQ=255)
                                                                cTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                      <  2:1357974/117‑1 (MQ=255)
                                                                  tttGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACa                                                                                   <  1:1415651/150‑1 (MQ=255)
                                                                      aCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATaaa                                                                               <  1:843440/150‑1 (MQ=255)
                                                                       ccAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCTCTTTCTTGCTACATGAACATAAAt                                                                              >  1:851616/1‑150 (MQ=255)
                                                                        cAGGTCTGACAGAGAGGTGCAGTGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATc                                                                             <  1:535253/150‑1 (MQ=255)
                                                                            tCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgc                                                                         >  2:1446448/1‑150 (MQ=255)
                                                                             cTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgcg                                                                        >  1:1313419/1‑150 (MQ=255)
                                                                                   gagagGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtct                                                                  <  1:737849/150‑1 (MQ=255)
                                                                                     gagGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtcttc                                                                >  1:769709/1‑150 (MQ=255)
                                                                                      agGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGATTTCTTGCTACATGAACATAAATCCTGCGGCGtcttct                                                               >  2:413740/1‑150 (MQ=255)
                                                                                       ggTGCAGGGTGAAAATTGCCATATTGTCCCGGGACGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCAACTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTa                                                              >  2:1414016/1‑150 (MQ=255)
                                                                                        gTGCAGGGTGAAAATTGCCATATTGTCCAGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAt                                                             <  2:419934/150‑1 (MQ=255)
                                                                                         tGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAtt                                                            >  2:311732/1‑150 (MQ=255)
                                                                                          gCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTc                                                           <  2:511893/150‑1 (MQ=255)
                                                                                          gCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTc                                                           <  1:399031/150‑1 (MQ=255)
                                                                                          gCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGAGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTc                                                           <  2:1456904/150‑1 (MQ=255)
                                                                                            aGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCAc                                                         <  1:934826/150‑1 (MQ=255)
                                                                                             gggTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACt                                                        <  2:1687578/150‑1 (MQ=255)
                                                                                             gggTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCATCTTCTATTCACt                                                        >  1:1360453/1‑150 (MQ=255)
                                                                                                   aaaTTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGAGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAg                                                  <  1:1692889/150‑1 (MQ=255)
                                                                                                      ttGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGcc                                               <  2:838584/150‑1 (MQ=255)
                                                                                                       ttccATATTTTCCCGGGATGGAACTCTCTATTCGTGTAAGCGGCTGCGTGTAGCCGCTATACAGCTCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                              <  2:984188/148‑1 (MQ=255)
                                                                                                       tcccAAATTGACCCGGGATTGAACGCTCTCATCGTGTAAGCGGCTGCCGGAAGCCGCTATACAGCGCGGTCACCTGGCTGAAAATCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                              <  2:81871/148‑1 (MQ=255)
                                                                                                       tGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                              >  1:1675584/1‑150 (MQ=255)
                                                                                                         ccATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCTCCTGGTTGAAATTCTTGATCGGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCa                                            <  2:733827/150‑1 (MQ=255)
                                                                                                          cATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCaa                                           >  1:1722310/1‑150 (MQ=255)
                                                                                                           atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                          <  2:984691/150‑1 (MQ=255)
                                                                                                           atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTAGACCGCGCGGTCACATGGTGGAACTTCTTGATCAGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAGTCACTACAAAGGCCGCAAg                                          >  1:81785/1‑150 (MQ=255)
                                                                                                            tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTAGACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGt                                         <  1:1013206/150‑1 (MQ=255)
                                                                                                             atTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGtt                                        >  1:1009258/1‑150 (MQ=255)
                                                                                                              ttGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTa                                       <  1:862334/150‑1 (MQ=255)
                                                                                                                 tCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTAccc                                    <  2:769709/150‑1 (MQ=255)
                                                                                                                   ccGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCGTTCTTGCGACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCa                                  >  1:1177384/1‑150 (MQ=255)
                                                                                                                     gggATCGAACGCTCAATTCCTGTAAGCGGCTGCGTGAAGCAGATATACAGCGCGGTCAGCTGGTTGATATTCTTCATCCGCTTTCTTGCTACATGAAAATAAATACTGAGTCCTCTTCTATTCACTACCAAGCCAGCAACTTAcaacata                                >  2:1216194/1‑144 (MQ=255)
                                                                                                                      ggATGTAACTCTCTATTCGTGTAAGCTGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAttt                               <  2:696947/150‑1 (MQ=255)
                                                                                                                          ggAACGCTCTATTCGTGTACGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTgcc                           >  2:1304621/1‑148 (MQ=255)
                                                                                                                          ggAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGAc                           >  2:1618688/1‑150 (MQ=255)
                                                                                                                           gAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACg                          <  2:74772/150‑1 (MQ=255)
                                                                                                                             aCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCa                        >  1:1187888/1‑150 (MQ=255)
                                                                                                                              cGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAg                       >  2:956094/1‑150 (MQ=255)
                                                                                                                                ctctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTg                     >  1:986644/1‑150 (MQ=255)
                                                                                                                                    tttCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATcc                 <  2:469728/149‑1 (MQ=255)
                                                                                                                                      tCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcg               <  2:1313419/150‑1 (MQ=255)
                                                                                                                                       ctgtataGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCGCTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGCTCCcgc              >  1:995250/7‑150 (MQ=255)
                                                                                                                                        gtgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcgcg             <  1:1232654/150‑1 (MQ=255)
                                                                                                                                          gtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa           >  1:832667/1‑150 (MQ=255)
                                                                                                                                          gtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa           >  1:386640/1‑150 (MQ=255)
                                                                                                                                           ataGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTAt          >  1:813352/3‑150 (MQ=255)
                                                                                                                                             aGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTg        <  2:1492793/150‑1 (MQ=255)
                                                                                                                                               cGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATACTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGc      >  2:1447497/1‑150 (MQ=255)
                                                                                                                                                ggCTGCGTGAAGCCGCTATACAGCGCGGGCACCTGGTTGAACTTCTTGATCCGCGTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCa     <  1:203443/150‑1 (MQ=255)
                                                                                                                                                 gCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCAc    <  2:744227/150‑1 (MQ=255)
                                                                                                                                                   tGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCATCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCg  <  2:1360453/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCG  >  NC_000913/891872‑892168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: