Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,023 T→C 6.5% R19R (CGT→CGC) ‡ rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,0230TC 6.5% 314.6 / 8.2 107R19R (CGT→CGC) ‡rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base T (46/53);  new base C (2/5);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 107 total aligned reads displayed.

ATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCC  >  NC_000913/891875‑892170
                                                                                                                                                    |                                                                                                                                                   
attTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTg                                                                                                                                                    >  2:1645427/1‑150 (MQ=255)
     cacaAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGcc                                                                                                                                               >  2:800178/1‑150 (MQ=255)
        aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                            <  2:373627/150‑1 (MQ=255)
        aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                            <  2:868692/150‑1 (MQ=255)
        aaaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                            <  2:601037/150‑1 (MQ=255)
        aaaCAGGGGTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCt                                                                                                                                            <  2:539773/150‑1 (MQ=255)
         aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGACCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                           >  2:881755/1‑150 (MQ=255)
         aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                           >  2:797221/1‑150 (MQ=255)
         aaCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCta                                                                                                                                           >  2:1025966/1‑150 (MQ=255)
          aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                          <  2:217384/150‑3 (MQ=255)
          aCAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                          <  1:1676115/150‑3 (MQ=255)
          aCAGGGTTGGGCGACGCGCTAAAACCGCCACGGCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTTCACGCAGCCGCtta                                                                                                                                          <  1:544757/150‑3 (MQ=255)
             tggTTGGGCGACGCGCTAAATGCGCCACGTCTATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCTTATTGTCCCGGGATGTAACGCTATATTCGTGTAATCGGCTTCACGCAGCCGCttacac                                                                                                                                       <  2:1491594/149‑6 (MQ=255)
             gggTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACa                                                                                                                                       >  1:1351715/1‑150 (MQ=255)
               gTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgc                                                                                                                                     <  1:1528822/150‑1 (MQ=255)
               gTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgc                                                                                                                                     >  1:840571/1‑150 (MQ=255)
                 tGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgcgc                                                                                                                                   <  1:11241/150‑1 (MQ=255)
                 tGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAgcgc                                                                                                                                   >  1:1687578/1‑150 (MQ=255)
                   ggCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCgg                                                                                                                                 <  2:490607/150‑1 (MQ=255)
                    gCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGCCAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGt                                                                                                                                >  2:862334/1‑150 (MQ=255)
                        cgcgCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCAcc                                                                                                                            >  1:511893/1‑150 (MQ=255)
                         gcgcTAAACCCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCt                                                                                                                           <  1:1071491/150‑1 (MQ=255)
                         gcgcTAAAACCGCCACGGCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGGGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCt                                                                                                                           <  1:482684/150‑1 (MQ=255)
                          cgcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                          >  1:989379/1‑150 (MQ=255)
                          cgcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTg                                                                                                                          >  1:838584/1‑150 (MQ=255)
                           gcTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCAGCTATACAGCGCGGTCACCTgg                                                                                                                         >  2:1480317/1‑150 (MQ=255)
                            cTAACACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGCACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                        <  1:1634124/150‑1 (MQ=255)
                            cTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGAGGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                        <  1:1627768/150‑1 (MQ=255)
                             tAAAACCGCCACGTCGAGGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGtt                                                                                                                       <  1:632403/150‑1 (MQ=255)
                              aaaaCCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTg                                                                                                                      >  1:887051/1‑150 (MQ=255)
                                  ccGCCACGTCGAGGTATGATACGCGGGCTTTTGTCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATAGAGCGGGGTCACCTGGTTGAAAt                                                                                                                  <  1:241727/150‑1 (MQ=255)
                                     ccACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCTTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                               <  2:557541/150‑1 (MQ=255)
                                     ccACGGCGCTGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCt                                                                                                               <  1:186806/150‑1 (MQ=255)
                                      cACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCtt                                                                                                              >  2:1035591/1‑150 (MQ=255)
                                          tCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATc                                                                                                          <  1:702371/150‑1 (MQ=255)
                                               gTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCtt                                                                                                     >  1:1008714/1‑150 (MQ=255)
                                                tATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCttt                                                                                                    >  2:1103186/1‑150 (MQ=255)
                                                 ttGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGGGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTc                                                                                                   <  2:692824/149‑1 (MQ=255)
                                                   gATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCtt                                                                                                 <  1:975937/150‑1 (MQ=255)
                                                      aCGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCt                                                                                              <  1:950129/150‑1 (MQ=255)
                                                       cgcgGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTa                                                                                             <  1:1026216/150‑1 (MQ=255)
                                                        gcgGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGGCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTAc                                                                                            <  2:80310/150‑1 (MQ=255)
                                                          gggCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACAt                                                                                          <  1:1487909/150‑1 (MQ=255)
                                                            gCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGa                                                                                        <  1:1103186/150‑1 (MQ=255)
                                                             cTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                        <  2:1357974/117‑1 (MQ=255)
                                                             cTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGt                                                                                                                        >  1:1357974/1‑117 (MQ=255)
                                                               tttGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACa                                                                                     <  1:1415651/150‑1 (MQ=255)
                                                                   aCCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATaaa                                                                                 <  1:843440/150‑1 (MQ=255)
                                                                    ccAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCTCTTTCTTGCTACATGAACATAAAt                                                                                >  1:851616/1‑150 (MQ=255)
                                                                     cAGGTCTGACAGAGAGGTGCAGTGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATc                                                                               <  1:535253/150‑1 (MQ=255)
                                                                         tCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgc                                                                           >  2:1446448/1‑150 (MQ=255)
                                                                          cTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgcg                                                                          >  1:1313419/1‑150 (MQ=255)
                                                                            gACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTgcggc                                                                        >  1:1492793/1‑150 (MQ=255)
                                                                                gagagGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtct                                                                    <  1:737849/150‑1 (MQ=255)
                                                                                  gagGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGtcttc                                                                  >  1:769709/1‑150 (MQ=255)
                                                                                   agGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGATTTCTTGCTACATGAACATAAATCCTGCGGCGtcttct                                                                 >  2:413740/1‑150 (MQ=255)
                                                                                    ggTGCAGGGTGAAAATTGCCATATTGTCCCGGGACGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCAACTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTa                                                                >  2:1414016/1‑150 (MQ=255)
                                                                                     gTGCAGGGTGAAAATTGCCATATTGTCCAGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAt                                                               <  2:419934/150‑1 (MQ=255)
                                                                                      tGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAtt                                                              >  2:311732/1‑150 (MQ=255)
                                                                                       gCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTc                                                             <  2:511893/150‑1 (MQ=255)
                                                                                       gCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGAGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTc                                                             <  2:1456904/150‑1 (MQ=255)
                                                                                         aGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCAc                                                           <  1:934826/150‑1 (MQ=255)
                                                                                          gggTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACt                                                          <  2:1687578/150‑1 (MQ=255)
                                                                                          gggTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCATCTTCTATTCACt                                                          >  1:1360453/1‑150 (MQ=255)
                                                                                                aaaTTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGAGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAg                                                    <  1:1692889/150‑1 (MQ=255)
                                                                                                   ttGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGcc                                                 <  2:838584/150‑1 (MQ=255)
                                                                                                    ttccATATTTTCCCGGGATGGAACTCTCTATTCGTGTAAGCGGCTGCGTGTAGCCGCTATACAGCTCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                                <  2:984188/148‑1 (MQ=255)
                                                                                                    tcccAAATTGACCCGGGATTGAACGCTCTCATCGTGTAAGCGGCTGCCGGAAGCCGCTATACAGCGCGGTCACCTGGCTGAAAATCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                                <  2:81871/148‑1 (MQ=255)
                                                                                                    tGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCg                                                >  1:1675584/1‑150 (MQ=255)
                                                                                                       cATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCaa                                             >  1:1722310/1‑150 (MQ=255)
                                                                                                        atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                            >  1:625880/1‑150 (MQ=255)
                                                                                                        atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAg                                            <  2:984691/150‑1 (MQ=255)
                                                                                                        atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTAACTACAAAGGCCGCaat                                            >  1:962280/1‑149 (MQ=255)
                                                                                                        atatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTAGACCGCGCGGTCACATGGTGGAACTTCTTGATCAGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTAGTCACTACAAAGGCCGCAAg                                            >  1:81785/1‑150 (MQ=255)
                                                                                                         tatTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTAGACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGt                                           <  1:1013206/150‑1 (MQ=255)
                                                                                                          atTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGtt                                          >  1:1009258/1‑150 (MQ=255)
                                                                                                           ttGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTa                                         <  1:862334/150‑1 (MQ=255)
                                                                                                              tCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTAccc                                      <  2:769709/150‑1 (MQ=255)
                                                                                                                ccGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCGTTCTTGCGACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCa                                    >  1:1177384/1‑150 (MQ=255)
                                                                                                                   ggATGTAACTCTCTATTCGTGTAAGCTGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCAttt                                 <  2:696947/150‑1 (MQ=255)
                                                                                                                       ggAACGCTCTATTCGTGTACGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTgcc                             >  2:1304621/1‑148 (MQ=255)
                                                                                                                       ggAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGAc                             >  2:1618688/1‑150 (MQ=255)
                                                                                                                        gAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACg                            <  2:74772/150‑1 (MQ=255)
                                                                                                                          aCGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCa                          >  1:1187888/1‑150 (MQ=255)
                                                                                                                           cGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAg                         >  2:956094/1‑150 (MQ=255)
                                                                                                                             ctctATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTg                       >  1:986644/1‑150 (MQ=255)
                                                                                                                                 tttCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATcc                   <  2:469728/149‑1 (MQ=255)
                                                                                                                                   tCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcg                 <  2:1313419/150‑1 (MQ=255)
                                                                                                                                    ctgtataGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCGCTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGCTCCcgc                >  1:995250/7‑150 (MQ=255)
                                                                                                                                     gtgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCcgcg               <  1:1232654/150‑1 (MQ=255)
                                                                                                                                       gtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa             >  1:832667/1‑150 (MQ=255)
                                                                                                                                       gtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTa             >  1:386640/1‑150 (MQ=255)
                                                                                                                                        ataGCGGCTTCACGCAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTAt            >  1:813352/3‑150 (MQ=255)
                                                                                                                                          aGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTg          <  2:1492793/150‑1 (MQ=255)
                                                                                                                                            cGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATACTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGc        >  2:1447497/1‑150 (MQ=255)
                                                                                                                                             ggCTGCGTGAAGCCGCTATACAGCGCGGGCACCTGGTTGAACTTCTTGATCCGCGTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCa       <  1:203443/150‑1 (MQ=255)
                                                                                                                                              gCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCAc      <  2:744227/150‑1 (MQ=255)
                                                                                                                                                tGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCATCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCg    <  2:1360453/150‑1 (MQ=255)
                                                                                                                                                  ggTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAAGATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGcc  <  2:244649/149‑1 (MQ=255)
                                                                                                                                                  cGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCACGTTACCCCATATTGACGCAGTGATCCCGCGTATTGGCACCGcc  >  1:469079/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTTGCACAAACAGGGTTGGGCGACGCGCTAAAACCGCCACGTCGATGTATGATACGCGGGCTTTTGACCAGGTCTGACAGAGAGGTGCAGGGTGAAAATTGCCATATTGTCCCGGGATGGAACGCTCTATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCC  >  NC_000913/891875‑892170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: