Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 912,583 T→A 6.9% E411V (GAG→GTG)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913912,5830TA 6.9% 228.8 / 4.8 82E411V (GAG→GTG) hcphybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base A (2/4);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

CATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTGTACG  >  NC_000913/912434‑912727
                                                                                                                                                     |                                                                                                                                                
cATCGTTACATTGACCTGCATCAACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGATAAAGCGATATTTACCACAGGCGAGGGTCAGGATAAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGTAGTAGTGGCGCt                                                                                                                                                  >  2:142484/1‑150 (MQ=255)
    gTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCTCAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTcgcc                                                                                                                                              <  1:237540/150‑1 (MQ=255)
         aTTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTg                                                                                                                                         >  1:1424611/1‑150 (MQ=255)
         aTTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTg                                                                                                                                         <  1:950471/150‑1 (MQ=255)
              ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACCGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                    >  1:881453/1‑150 (MQ=255)
              ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                    >  1:373338/1‑150 (MQ=255)
              ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGAGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                    <  2:1645248/150‑1 (MQ=255)
               cTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccac                                                                                                                                   <  2:537656/150‑3 (MQ=255)
                tGCAGCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGGCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccact                                                                                                                                  <  2:716435/150‑4 (MQ=255)
                 gCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGATGTAGTGGCGCTCGCCGCGTGCGCCGTcc                                                                                                                                 >  1:72577/1‑149 (MQ=255)
                    tCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactactt                                                                                                                              <  2:706574/150‑8 (MQ=255)
                      tACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGcc                                                                                                                            >  1:1640836/1‑150 (MQ=255)
                      tACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTAACACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACCGcc                                                                                                                            >  2:85289/1‑150 (MQ=255)
                         cAGCCGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCCCAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGccacc                                                                                                                         >  2:1330227/1‑150 (MQ=255)
                         cAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGccacc                                                                                                                         >  2:772410/1‑150 (MQ=255)
                           caCGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCaa                                                                                                                       <  1:1480445/149‑1 (MQ=255)
                             cgcgGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGc                                                                                                                     <  2:891595/150‑1 (MQ=255)
                                    gACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGa                                                                                                              >  1:1884/1‑150 (MQ=255)
                                     aCCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGat                                                                                                             <  1:1441369/150‑1 (MQ=255)
                                     aCCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGCCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAATGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGat                                                                                                             >  1:440323/1‑150 (MQ=255)
                                       cTTCGATATCGCCAAACTCAAGTTTGATAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGACATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGatat                                                                                                           >  1:411236/1‑150 (MQ=255)
                                        ttCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGGGGCGAAATCGGTGAAGTCGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATg                                                                                                          >  2:253395/1‑150 (MQ=255)
                                         tCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGa                                                                                                         >  1:909627/1‑150 (MQ=255)
                                           gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACg                                                                                                       >  2:1486572/1‑150 (MQ=255)
                                           gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACg                                                                                                       <  2:976399/150‑1 (MQ=255)
                                           gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactact                                                                                                                               >  2:1442365/1‑120 (MQ=255)
                                           gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactact                                                                                                                               <  1:1442365/126‑7 (MQ=255)
                                              atCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAgg                                                                                                                  <  2:1054318/136‑1 (MQ=255)
                                              atCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAgg                                                                                                                  >  1:1054318/1‑136 (MQ=255)
                                                    aaaCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCCGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTc                                                                                              <  1:1324871/150‑1 (MQ=255)
                                                     aaCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCa                                                                                             <  1:222552/150‑1 (MQ=255)
                                                     aaCTCAAGTTTGTTAAAGCCATATTTACCACAGGCCAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGAGGCGAAATCGGTGAAGTAGAGGCGCTCCCCGCGTGCGCCGTCCCAGCCACCAAGCAGGAAGATATGACGCAGTGTTTCa                                                                                             >  2:1611731/1‑150 (MQ=255)
                                                      aCTCAAGTTTGTTAAAGCGATATTTACCCCAGGCGAGGGGCCGGATCAGGCTGTCAGCCGGCACGCTGGGGGCGAAATCGGTGAAGTAGTGGCGCGCGCCGCGTTCGCCGTCACCTCCCCCAAGCAGGAAGATATGACGCAGTTTATCAc                                                                                            <  1:281475/150‑1 (MQ=255)
                                                      aCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCAc                                                                                            >  1:493027/1‑150 (MQ=255)
                                                      aCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCAc                                                                                            <  1:36315/150‑1 (MQ=255)
                                                       cTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAAGCGGGGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACg                                                                                           <  2:1208050/150‑1 (MQ=255)
                                                         cAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGc                                                                                         >  1:501278/1‑150 (MQ=255)
                                                         cAAGTTTGTTAAAGCGATATTTACCACAGCCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGc                                                                                         <  2:298724/150‑1 (MQ=255)
                                                               tGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCa                                                                                   >  2:7975/1‑150 (MQ=255)
                                                                   aaaGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatc                                                                               <  2:208877/150‑1 (MQ=255)
                                                                     aGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCTGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaa                                                                             >  2:794569/1‑150 (MQ=255)
                                                                     aGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaa                                                                             >  2:778967/1‑150 (MQ=255)
                                                                        gATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaatca                                                                          <  1:1486572/150‑1 (MQ=255)
                                                                          tatTTACCACAGGCGAGGGTCATGATCAGGCAGTCATCCGGCACGCTGGTGGCGATATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGc                                                                        <  1:1436472/150‑1 (MQ=255)
                                                                           atTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                       <  2:80045/150‑1 (MQ=255)
                                                                           atTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                       >  1:1583536/1‑150 (MQ=255)
                                                                             ttACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTa                                                                     >  2:945284/1‑150 (MQ=255)
                                                                                ccACAGGCGAGGGGCAGGATCAGGCTGTCATCCGGCACGCGGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATca                                                                  <  2:208934/150‑1 (MQ=255)
                                                                                  acaGGCGAGGGTCAGGATCAGGTAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGCCTCACCAGAACAATCAGCGTATcagc                                                                >  1:520456/1‑150 (MQ=255)
                                                                                    aGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATcagcag                                                              <  2:1276602/150‑1 (MQ=255)
                                                                                     ggCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCagcagc                                                             >  2:1551569/1‑150 (MQ=255)
                                                                                     ggCGAGGGTCAGGATCAGGCAGTCATCCGCCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                       >  2:441884/1‑140 (MQ=255)
                                                                                     ggCGAGGGTCAGGATCAGGCAGTCATCCGCCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                       <  1:441884/140‑1 (MQ=255)
                                                                                      gCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAgcg                                                            <  1:521311/150‑1 (MQ=255)
                                                                                          gggTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCaa                                                        >  1:793106/1‑150 (MQ=255)
                                                                                            gTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCCCCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAagc                                                      >  2:1351801/1‑150 (MQ=255)
                                                                                            gTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGCTATGCCGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAagc                                                      >  1:582655/1‑150 (MQ=255)
                                                                                                  aTCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTc                                                <  1:1228205/150‑1 (MQ=255)
                                                                                                      ggCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGc                                            >  2:1027/1‑150 (MQ=255)
                                                                                                        cAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGa                                          <  1:1689127/150‑1 (MQ=255)
                                                                                                         aGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAc                                         >  1:941611/1‑150 (MQ=255)
                                                                                                         aGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAc                                         <  1:586438/150‑1 (MQ=255)
                                                                                                          gTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                        >  1:374247/1‑150 (MQ=255)
                                                                                                          gTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                        <  1:40356/150‑1 (MQ=255)
                                                                                                          gTCATCCGGCACGCTGGGGGCGAAATCGGTGAAGTAGTGGCTCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                        <  2:72577/150‑1 (MQ=255)
                                                                                                           tCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCTTATCAGCAGCGCCAAGCAGCGTCTGGCGACCa                                       >  1:1044280/1‑150 (MQ=255)
                                                                                                              tCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCaaaa                                    <  1:1167786/150‑1 (MQ=255)
                                                                                                                cGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCCCCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCCGCGTCTGGCGCCCAAAAcc                                  >  1:1533600/1‑150 (MQ=255)
                                                                                                                   cACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCCAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGCTCAATCAGCGTATCAGCAGCGCCACGCAGCGTCTGGCGACCAAAACCCAc                               >  1:1425072/1‑150 (MQ=255)
                                                                                                                    aCGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACg                              <  2:1583536/150‑1 (MQ=255)
                                                                                                                         ggtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAACCCCACGGTGAt                         >  1:1657239/1‑150 (MQ=255)
                                                                                                                         ggtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGATCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGAt                         <  1:89236/150‑1 (MQ=255)
                                                                                                                          gtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGCCGCAGTGTTTCACGGCTGACAAGATCAATAAGCGTATCAGCAGCGCCAAGCAGCGGCTGGCGACCAAACCCCACGGTtata                        >  2:258659/1‑150 (MQ=255)
                                                                                                                          gtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATa                        <  1:778967/150‑1 (MQ=255)
                                                                                                                               gAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTg                   <  2:493027/150‑1 (MQ=255)
                                                                                                                                  aTCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGGGCCCAAAACCCACGGGGATAAGGTGCgg                >  2:1039994/1‑150 (MQ=255)
                                                                                                                                          agtagtGGCGCTCGCCGCGTGCGCTGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGc        <  1:794569/150‑1 (MQ=255)
                                                                                                                                          agtagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGc        <  1:1303896/150‑1 (MQ=255)
                                                                                                                                           gtagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCCGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCCCGGTGCTAAGGTGCGGCATTTCGcg       >  1:313078/1‑149 (MQ=255)
                                                                                                                                            tagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTg      <  2:1226438/150‑1 (MQ=255)
                                                                                                                                            tagtGGCGCTCGCCGCGTGCGCCGGCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTg      <  2:346727/150‑1 (MQ=255)
                                                                                                                                             gacggCGCACGCGGCGAGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCTAGCAGCGTCTGGCGGCCAAAACCCACGGTCAGAAGGTGCGGAATTTCGCTGt     >  2:1478087/4‑150 (MQ=255)
                                                                                                                                                ggCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGAGGCAGATTTTCACGGCTCCCCAGAACAATCAGCGTCTCAGCAGCGCCAAGCCGCGTCTGGCGACCAAAACCCACGGTGATAAGGAGGGGAATTTCGCTGTACg  >  2:1268379/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CATCGTTACATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTGTACG  >  NC_000913/912434‑912727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: