Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 912,591 T→A 6.9% A408A (GCA→GCT hcp ← hybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913912,5910TA 6.9% 238.5 / 6.1 87A408A (GCA→GCThcphybrid‑cluster [4Fe‑2S‑2O] subunit of anaerobic terminal reductases
Reads supporting (aligned to +/- strand):  ref base T (42/39);  new base A (2/4);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

ATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTGTACGGGAAGCCTGCCAT  >  NC_000913/912443‑912740
                                                                                                                                                    |                                                                                                                                                     
aTTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTg                                                                                                                                                      >  1:1424611/1‑150 (MQ=255)
aTTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTg                                                                                                                                                      <  1:950471/150‑1 (MQ=255)
     ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACCGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                                 >  1:881453/1‑150 (MQ=255)
     ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                                 >  1:373338/1‑150 (MQ=255)
     ccTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGAGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCg                                                                                                                                                 <  2:1645248/150‑1 (MQ=255)
      cTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccac                                                                                                                                                <  2:537656/150‑3 (MQ=255)
       tGCAGCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGGCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccact                                                                                                                                               <  2:716435/150‑4 (MQ=255)
        gCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGATGTAGTGGCGCTCGCCGCGTGCGCCGTcc                                                                                                                                              >  1:72577/1‑149 (MQ=255)
           tCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactactt                                                                                                                                           <  2:706574/150‑8 (MQ=255)
             tACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGcc                                                                                                                                         >  1:1640836/1‑150 (MQ=255)
             tACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTAACACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACCGcc                                                                                                                                         >  2:85289/1‑150 (MQ=255)
                cAGCCGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCCCAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGccacc                                                                                                                                      >  2:1330227/1‑150 (MQ=255)
                cAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGccacc                                                                                                                                      >  2:772410/1‑150 (MQ=255)
                  caCGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCaa                                                                                                                                    <  1:1480445/149‑1 (MQ=255)
                    cgcgGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGc                                                                                                                                  <  2:891595/150‑1 (MQ=255)
                           gACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGa                                                                                                                           >  1:1884/1‑150 (MQ=255)
                            aCCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGat                                                                                                                          <  1:1441369/150‑1 (MQ=255)
                            aCCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGCCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAATGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGat                                                                                                                          >  1:440323/1‑150 (MQ=255)
                              cTTCGATATCGCCAAACTCAAGTTTGATAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGACATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGatat                                                                                                                        >  1:411236/1‑150 (MQ=255)
                               ttCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGGGGCGAAATCGGTGAAGTCGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATg                                                                                                                       >  2:253395/1‑150 (MQ=255)
                                tCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGa                                                                                                                      >  1:909627/1‑150 (MQ=255)
                                  gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACg                                                                                                                    >  2:1486572/1‑150 (MQ=255)
                                  gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACg                                                                                                                    <  2:976399/150‑1 (MQ=255)
                                  gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactact                                                                                                                                            >  2:1442365/1‑120 (MQ=255)
                                  gATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCACGCGGCGAGCGccactact                                                                                                                                            <  1:1442365/126‑7 (MQ=255)
                                     atCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAgg                                                                                                                               >  1:1054318/1‑136 (MQ=255)
                                     atCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAgg                                                                                                                               <  2:1054318/136‑1 (MQ=255)
                                           aaaCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCCGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTc                                                                                                           <  1:1324871/150‑1 (MQ=255)
                                            aaCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCa                                                                                                          <  1:222552/150‑1 (MQ=255)
                                            aaCTCAAGTTTGTTAAAGCCATATTTACCACAGGCCAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGAGGCGAAATCGGTGAAGTAGAGGCGCTCCCCGCGTGCGCCGTCCCAGCCACCAAGCAGGAAGATATGACGCAGTGTTTCa                                                                                                          >  2:1611731/1‑150 (MQ=255)
                                             aCTCAAGTTTGTTAAAGCGATATTTACCCCAGGCGAGGGGCCGGATCAGGCTGTCAGCCGGCACGCTGGGGGCGAAATCGGTGAAGTAGTGGCGCGCGCCGCGTTCGCCGTCACCTCCCCCAAGCAGGAAGATATGACGCAGTTTATCAc                                                                                                         <  1:281475/150‑1 (MQ=255)
                                             aCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCAc                                                                                                         <  1:36315/150‑1 (MQ=255)
                                             aCTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCAc                                                                                                         >  1:493027/1‑150 (MQ=255)
                                              cTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAAGCGGGGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACg                                                                                                        <  2:1208050/150‑1 (MQ=255)
                                                cAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGc                                                                                                      >  1:501278/1‑150 (MQ=255)
                                                cAAGTTTGTTAAAGCGATATTTACCACAGCCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGc                                                                                                      <  2:298724/150‑1 (MQ=255)
                                                      tGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCa                                                                                                >  2:7975/1‑150 (MQ=255)
                                                          aaaGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatc                                                                                            <  2:208877/150‑1 (MQ=255)
                                                            aGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCTGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaa                                                                                          >  2:794569/1‑150 (MQ=255)
                                                            aGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaa                                                                                          >  2:778967/1‑150 (MQ=255)
                                                               gATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGatcaatca                                                                                       <  1:1486572/150‑1 (MQ=255)
                                                                 tatTTACCACAGGCGAGGGTCATGATCAGGCAGTCATCCGGCACGCTGGTGGCGATATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGc                                                                                     <  1:1436472/150‑1 (MQ=255)
                                                                  atTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                                    <  2:80045/150‑1 (MQ=255)
                                                                  atTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                                    >  1:1583536/1‑150 (MQ=255)
                                                                    ttACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTa                                                                                  >  2:945284/1‑150 (MQ=255)
                                                                       ccACAGGCGAGGGGCAGGATCAGGCTGTCATCCGGCACGCGGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATca                                                                               <  2:208934/150‑1 (MQ=255)
                                                                         acaGGCGAGGGTCAGGATCAGGTAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGCCTCACCAGAACAATCAGCGTATcagc                                                                             >  1:520456/1‑150 (MQ=255)
                                                                           aGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATcagcag                                                                           <  2:1276602/150‑1 (MQ=255)
                                                                            ggCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCagcagc                                                                          >  2:1551569/1‑150 (MQ=255)
                                                                            ggCGAGGGTCAGGATCAGGCAGTCATCCGCCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                                    <  1:441884/140‑1 (MQ=255)
                                                                            ggCGAGGGTCAGGATCAGGCAGTCATCCGCCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCg                                                                                    >  2:441884/1‑140 (MQ=255)
                                                                             gCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAgcg                                                                         <  1:521311/150‑1 (MQ=255)
                                                                                 gggTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCaa                                                                     >  1:793106/1‑150 (MQ=255)
                                                                                   gTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCCCCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAagc                                                                   >  2:1351801/1‑150 (MQ=255)
                                                                                   gTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGCTATGCCGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAagc                                                                   >  1:582655/1‑150 (MQ=255)
                                                                                         aTCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTc                                                             <  1:1228205/150‑1 (MQ=255)
                                                                                             ggCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGc                                                         >  2:1027/1‑150 (MQ=255)
                                                                                               cAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGa                                                       <  1:1689127/150‑1 (MQ=255)
                                                                                                aGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAc                                                      >  1:941611/1‑150 (MQ=255)
                                                                                                aGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAc                                                      <  1:586438/150‑1 (MQ=255)
                                                                                                 gTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                                     >  1:374247/1‑150 (MQ=255)
                                                                                                 gTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                                     <  1:40356/150‑1 (MQ=255)
                                                                                                 gTCATCCGGCACGCTGGGGGCGAAATCGGTGAAGTAGTGGCTCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGAcc                                                     <  2:72577/150‑1 (MQ=255)
                                                                                                  tCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCTTATCAGCAGCGCCAAGCAGCGTCTGGCGACCa                                                    >  1:1044280/1‑150 (MQ=255)
                                                                                                     tCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCaaaa                                                 <  1:1167786/150‑1 (MQ=255)
                                                                                                       cGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCCCCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCCGCGTCTGGCGCCCAAAAcc                                               >  1:1533600/1‑150 (MQ=255)
                                                                                                          cACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCCAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGCTCAATCAGCGTATCAGCAGCGCCACGCAGCGTCTGGCGACCAAAACCCAc                                            >  1:1425072/1‑150 (MQ=255)
                                                                                                           aCGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACg                                           <  2:1583536/150‑1 (MQ=255)
                                                                                                                ggtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAACCCCACGGTGAt                                      >  1:1657239/1‑150 (MQ=255)
                                                                                                                ggtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGATCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGAt                                      <  1:89236/150‑1 (MQ=255)
                                                                                                                 gtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGCCGCAGTGTTTCACGGCTGACAAGATCAATAAGCGTATCAGCAGCGCCAAGCAGCGGCTGGCGACCAAACCCCACGGTtata                                     >  2:258659/1‑150 (MQ=255)
                                                                                                                 gtggCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATa                                     <  1:778967/150‑1 (MQ=255)
                                                                                                                      gAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTg                                <  2:493027/150‑1 (MQ=255)
                                                                                                                         aTCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGGGCCCAAAACCCACGGGGATAAGGTGCgg                             >  2:1039994/1‑150 (MQ=255)
                                                                                                                                 agtagtGGCGCTCGCCGCGTGCGCTGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGc                     <  1:794569/150‑1 (MQ=255)
                                                                                                                                 agtagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGc                     <  1:1303896/150‑1 (MQ=255)
                                                                                                                                  gtagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCCGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCCCGGTGCTAAGGTGCGGCATTTCGcg                    >  1:313078/1‑149 (MQ=255)
                                                                                                                                   tagtGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTg                   <  2:1226438/150‑1 (MQ=255)
                                                                                                                                   tagtGGCGCTCGCCGCGTGCGCCGGCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTg                   <  2:346727/150‑1 (MQ=255)
                                                                                                                                    gacggCGCACGCGGCGAGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCTAGCAGCGTCTGGCGGCCAAAACCCACGGTCAGAAGGTGCGGAATTTCGCTGt                  >  2:1478087/4‑150 (MQ=255)
                                                                                                                                       ggCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGAGGCAGATTTTCACGGCTCCCCAGAACAATCAGCGTCTCAGCAGCGCCAAGCCGCGTCTGGCGACCAAAACCCACGGTGATAAGGAGGGGAATTTCGCTGTACg               >  2:1268379/1‑150 (MQ=255)
                                                                                                                                          gcgcgccgcGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGCGCGGAATTTCGCTGTACGGGa            <  1:142484/147‑1 (MQ=255)
                                                                                                                                             cgccgcGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACGGGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAAATTCGCTGTACGGGAAGc         >  1:563208/1‑150 (MQ=255)
                                                                                                                                               ccgcGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGCCGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCCGCGCCACGCAGCGTCTGGCGACCACAACCGACGGGGATAAGGTGCGGAATTTCGCTGTACGGGAAGccg       >  1:1525300/1‑149 (MQ=255)
                                                                                                                                                cgctTGCGCCGTCACAGGCTCCAAGGAGGAAGATATGACGGAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAAGCCACGGTGATAAGGTGCGGAATTTCGCTGTACGGGAAGCCTg      <  2:495114/150‑1 (MQ=255)
                                                                                                                                                cgcgTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTGTACGGGAAGCCTg      <  2:411236/150‑1 (MQ=255)
                                                                                                                                                 gcgTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGAGGAATTTCGCTGTACGGGAAGCCTGc     <  2:440323/150‑1 (MQ=255)
                                                                                                                                                    tGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGCTGCGGAATTTCGCTGTACGGGACGCCTGCCAt  >  1:1355767/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATTGACCTGCATCTACCAGACGCGGCAGACCTTCGATATCGCCAAACTCAAGTTTGTTAAAGCGATATTTACCACAGGCGAGGGTCAGGATCAGGCAGTCATCCGGCACGCTGGTGGCGAAATCGGTGAAGTAGTGGCGCTCGCCGCGTGCGCCGTCACAGCCACCAAGCAGGAAGATATGACGCAGTTTTTCACGGCTCACCAGATCAATCAGCGTATCAGCAGCGCCAAGCAGCGTCTGGCGACCAAAACCCACGGTGATAAGGTGCGGAATTTCGCTGTACGGGAAGCCTGCCAT  >  NC_000913/912443‑912740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: