Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 940,751 T→C 9.8% intergenic (+31/‑208) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,7510TC 9.8% 364.2 / 22.8 132intergenic (+31/‑208)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (69/50);  new base C (9/4);  total (78/54)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-01

Only 100 of 132 total aligned reads displayed.

CTGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAG  >  NC_000913/940604‑940896
                                                                                                                                                   |                                                                                                                                                 
ctggCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGc                                                                                                                                                 >  2:429973/1‑150 (MQ=255)
  ggCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCgg                                                                                                                                               >  1:1489376/1‑150 (MQ=255)
         gggcGTACGCTGGTTGCAGTTATGGAATACTATCAGCAGGCTGATGGTCGTATTGAAGTACAAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttt                                                                                                                                        <  1:1259732/147‑1 (MQ=255)
         ggTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttt                                                                                                                                        >  1:90686/1‑150 (MQ=255)
            cGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCTTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCttttta                                                                                                                                     <  1:458295/150‑2 (MQ=255)
             gTACGCTGGTTGCAGTAATGGAAATCTATCTGCATGCTGTTGGTCGTTTTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATCTTGGCTAATACCCAATTTTTCTGAATCTATAAAGCGCCCGCAGGCGCtttttag                                                                                                                                    <  1:315234/150‑3 (MQ=255)
             gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATCTGAACGGACGGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                                                                    <  1:315186/150‑1 (MQ=255)
             gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCTGGCGCttttttt                                                                                                                                    >  1:858854/1‑150 (MQ=255)
             gTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCttttttt                                                                                                                                    <  2:1400812/150‑1 (MQ=255)
                cGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                 <  2:128301/150‑6 (MQ=255)
                cGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCCGCAGGCGCtttttagatt                                                                                                                                 <  2:139271/150‑6 (MQ=255)
                 gCTGGTGTCAGTAATGGAAATCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtct                                                                                                                                <  2:875656/150‑1 (MQ=255)
                  cTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGtctc                                                                                                                               >  2:1115299/1‑150 (MQ=255)
                         cAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATATTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTACCTTTGa                                                                                                                        >  1:1290284/1‑150 (MQ=255)
                           gTAATGGAAAACTATCAGCAGGCTGATGGGCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATa                                                                                                                      >  1:1156255/1‑150 (MQ=255)
                              aTGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCg                                                                                                                   >  1:992600/1‑150 (MQ=255)
                                ggAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGaa                                                                                                                 >  2:1500273/1‑150 (MQ=255)
                                 gAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGAATGCGGGCGCTTTTTTTGTGTCACTTTGATAcataac                                                                                                                >  1:1043940/1‑145 (MQ=255)
                                 gAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCAGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCATTTGATACCGAAc                                                                                                                >  1:1652062/1‑150 (MQ=255)
                                   aaaCTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATATAAAAAGCGCATGCGGGCGCTTTTTTTGTATCCCTTTCATACCGAACaa                                                                                                              >  2:110386/1‑150 (MQ=255)
                                      cTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACaataa                                                                                                           >  2:1190711/1‑150 (MQ=255)
                                        aTCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                                                                         >  1:767499/1‑150 (MQ=255)
                                        aTCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAAtt                                                                                                         >  1:717732/1‑150 (MQ=255)
                                         tCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTa                                                                                                        >  1:449713/1‑150 (MQ=255)
                                             cagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcc                                                                                                    >  1:1213300/1‑150 (MQ=255)
                                             cagGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTActcc                                                                                                    >  1:1608139/1‑150 (MQ=255)
                                                gCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCa                                                                                                 >  2:1467289/1‑150 (MQ=255)
                                                     tGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTTGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTac                                                                                            <  2:520300/150‑1 (MQ=255)
                                                       gTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTacac                                                                                          >  2:116754/1‑150 (MQ=255)
                                                       gTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTacac                                                                                          >  2:1676589/1‑150 (MQ=255)
                                                           tATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTACTCACTTACACGTaa                                                                                      >  1:1383683/1‑150 (MQ=255)
                                                            aTTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAt                                                                                     <  2:1614382/150‑1 (MQ=255)
                                                             ttGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAta                                                                                    <  2:1250518/150‑1 (MQ=255)
                                                             ttGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAta                                                                                    >  2:768125/1‑150 (MQ=255)
                                                              tGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTGTACCTTTGATACCGAAAAATAATTACACCTCACTTACACGTAAtaa                                                                                   >  1:1596836/1‑149 (MQ=255)
                                                               gAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAAtact                                                                                  <  1:1163007/150‑1 (MQ=255)
                                                               gAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGAGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTATtact                                                                                  >  1:213542/1‑150 (MQ=255)
                                                                  gTACCAGAAGTTCTGCGTCCGTATATGAACGGACGGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATactact                                                                               <  1:645030/150‑1 (MQ=255)
                                                                   tACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACtt                                                                              >  1:1042231/1‑150 (MQ=255)
                                                                    aacAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTATGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTATACCTTTGATACCGAACAATAAATACTCCTAACTTAAACGTAATACTACttt                                                                             >  2:608761/3‑150 (MQ=255)
                                                                    aCCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACttt                                                                             >  1:561084/1‑150 (MQ=255)
                                                                      cAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCg                                                                           >  1:1186956/1‑150 (MQ=255)
                                                                        taaGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGTCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                                                                         <  1:1598177/149‑1 (MQ=255)
                                                                        gAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGTATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                                                                         <  2:1492613/150‑1 (MQ=255)
                                                                        gAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAg                                                                         <  1:793151/150‑1 (MQ=255)
                                                                          aGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTTTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTAGAGtt                                                                       >  1:1647558/1‑149 (MQ=255)
                                                                          aGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTg                                                                       <  2:898734/150‑1 (MQ=255)
                                                                          aGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTg                                                                       >  2:1128801/1‑150 (MQ=255)
                                                                           gTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGa                                                                      >  2:1371896/1‑150 (MQ=255)
                                                                           gTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGa                                                                      <  1:1467289/150‑1 (MQ=255)
                                                                            ttCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGaa                                                                     >  2:58813/1‑150 (MQ=255)
                                                                               tGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTATGCCTTTGATACCGAACAATAATTACTCCGCACTTACACGTAATACTACTTTCGATTGAAAAt                                                                  >  1:1604906/1‑150 (MQ=255)
                                                                                gCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATc                                                                 <  1:185922/150‑1 (MQ=255)
                                                                                    tagTATATGAACTGTCTGGAATATTTTTGCTAATACACAATTTTTCTGAATCTAAAAATCGCCTTCTGGCGCTTTTTTTGTCTATCTTTGATACCGAACAATAATTACTCCTCACTTTCACGTAATACTACTTTCGAGTGAAAATCTAcc                                                             <  1:178508/148‑1 (MQ=255)
                                                                                     cGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCt                                                            >  1:227923/1‑150 (MQ=255)
                                                                                      gTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCCAACAATAATTAATCCTCACGTACACGTAATACTACTTTAGAGTGAAATactaacta                                                           >  1:1420732/1‑150 (MQ=255)
                                                                                      gTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGAGCGTTTTTTGTATCCCTTTGATACCGAACAATAATTACTCCTCAATTACACGTAATACTACTTTCGAGTGAAAATCTACCTa                                                           >  1:1062453/1‑150 (MQ=255)
                                                                                       tataTGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAATAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTAt                                                          <  1:1253126/150‑1 (MQ=255)
                                                                                            gAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCtt                                                     >  1:607524/1‑150 (MQ=255)
                                                                                            gAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCtt                                                     >  2:11679/1‑150 (MQ=255)
                                                                                             aaCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATAACCAACAATAATTACACCGCACATACACGTAATACTACTTTCGAGTGAAAATCTACCAATATAttt                                                    >  2:1317762/1‑150 (MQ=255)
                                                                                             aaCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGATTTTTTTGTCTCCCTTTGATACCGAACAATAATACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTg                                                   <  1:1015069/150‑1 (MQ=255)
                                                                                              aCGGACTGGAATATATTGTCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTg                                                   <  1:157484/150‑1 (MQ=255)
                                                                                              aCGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAACATCTACCTATCTCTTTg                                                   >  1:827757/1‑150 (MQ=255)
                                                                                               cGGACTGGAATATATTTGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGa                                                  <  1:24382/150‑1 (MQ=255)
                                                                                                ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                 <  1:1157320/150‑1 (MQ=255)
                                                                                                ggACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGAt                                                 >  2:185075/1‑150 (MQ=255)
                                                                                                   cTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTc                                             >  1:1167322/1‑150 (MQ=255)
                                                                                                     ggAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTGTTTTTGTATCCCTTTTAAAACGAAAAATAAATAATCCTCACTTACACGTAAATCTACTTTCGAGTGAAAATCTACCTATCTCTTTTAttttaa                                            >  1:1474431/1‑148 (MQ=255)
                                                                                                      gAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCaa                                           <  2:1213300/150‑1 (MQ=255)
                                                                                                      gAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCaa                                           <  2:1608139/150‑1 (MQ=255)
                                                                                                             ttcgCTAATACACAATTTATCTGAATCAAAAAACCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTc                                    <  2:694148/147‑1 (MQ=255)
                                                                                                                 cTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATg                                <  2:311067/150‑1 (MQ=255)
                                                                                                                   aaTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGta                              <  1:1341688/150‑1 (MQ=255)
                                                                                                                    aTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGCACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGtat                             >  1:1638503/1‑150 (MQ=255)
                                                                                                                       cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACa                          <  1:32758/150‑1 (MQ=255)
                                                                                                                       cccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACa                          >  2:1611073/1‑150 (MQ=255)
                                                                                                                        ccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACaa                         >  2:880182/1‑150 (MQ=255)
                                                                                                                          aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGc                       <  2:449713/150‑1 (MQ=255)
                                                                                                                           aTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGcc                      <  2:200785/150‑1 (MQ=255)
                                                                                                                           aTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGcc                      <  1:1115299/150‑1 (MQ=255)
                                                                                                                           aTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGGCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTCTCTCTTTGATTTTCAAATTATTCGATGTATACAAGcc                      <  2:143289/150‑1 (MQ=255)
                                                                                                                             ttttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCta                    <  2:1178608/150‑1 (MQ=255)
                                                                                                                                tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCtatat                 <  2:1489376/150‑1 (MQ=255)
                                                                                                                                  tGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAg               >  1:510728/1‑150 (MQ=255)
                                                                                                                                   gAATCTAAAAAGCGCCTGCGGGCGCGTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGc              >  1:464930/1‑150 (MQ=255)
                                                                                                                                   gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTGTCTCCCTTAGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg             <  2:289860/150‑1 (MQ=255)
                                                                                                                                    gacaaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCg             >  1:1388435/6‑150 (MQ=255)
                                                                                                                                      caaaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGaa           >  2:614321/4‑150 (MQ=255)
                                                                                                                                        tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGCTACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt         >  1:1410184/1‑150 (MQ=255)
                                                                                                                                        tAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGAAACCGAACAATAATTACTCCTACCGTACACGTAATACTACATTAGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt         >  2:128673/1‑150 (MQ=255)
                                                                                                                                        aaaaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACt         >  2:651603/2‑150 (MQ=255)
                                                                                                                                         aaaaaGCGCCTGCGGGCGCTTTTTTTGACTCCCTTTGATACCGAAAAAAAATTACTCCACACTTACACGTAAAACTAATTACGAGTTAAAATATAACAATCTATTTTATTTTCAAATTATTCTATGTATACAAGCCTAAATATCGAACTg        >  2:1554926/1‑150 (MQ=255)
                                                                                                                                          aaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGATCAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGAGAACTGc       >  1:154276/1‑150 (MQ=255)
                                                                                                                                          aaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCCCTTACACGTAATACTACTTTCGAGTGAAAAGCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATTCAAGCCTATATAGCGAACTGc       >  1:1510584/1‑150 (MQ=255)
                                                                                                                                          aaaaGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGc       >  1:1719736/1‑150 (MQ=255)
                                                                                                                                             aGCGCCCGCAGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACATACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCtat    >  2:1441195/1‑150 (MQ=255)
                                                                                                                                               cgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAg  <  2:1718117/150‑1 (MQ=255)
                                                                                                                                               cgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAg  >  1:922047/1‑150 (MQ=255)
                                                                                                                                               cgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACATTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTAGATGTATACAATCCTATATAGCGAAATGCtatat  >  1:691139/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGGCTGTTGGTCGTACGCTGGTTGCAGTAATGGAAAACTATCAGCAGGCTGATGGTCGTATTGAAGTACCAGAAGTTCTGCGTCCGTATATGAACGGACTGGAATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAG  >  NC_000913/940604‑940896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: