Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 958,771 A→T 6.5% intergenic (+30/‑41) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913958,7710AT 6.5% 298.3 / 8.9 107intergenic (+30/‑41)serC/aroA3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
Reads supporting (aligned to +/- strand):  ref base A (48/52);  new base T (4/3);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 113 total aligned reads displayed.

CTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACA  >  NC_000913/958625‑958918
                                                                                                                                                  |                                                                                                                                                   
      ggTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTggg                                                                                                                                            >  2:1568621/1‑150 (MQ=255)
         cACCGTGTGTTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTggggat                                                                                                                                         <  2:255756/150‑3 (MQ=255)
         cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtt                                                                                                                                         >  2:680883/1‑150 (MQ=255)
         cACCGGGTGGTCGGCGGAATGCGCGCTTCTATTTATAACCCCATGCCGCTCGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGGTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTggggat                                                                                                                                         <  1:338817/150‑3 (MQ=255)
          aCCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttt                                                                                                                                        >  1:848531/1‑150 (MQ=255)
           ccGTGTGGTCGGCGGAATGCGCGCTGCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtttt                                                                                                                                       <  2:1152212/150‑1 (MQ=255)
              tgtgGTCGGCGGATTGCGCGCTTATATTTATAACGACATGCCGCTGGAAGGCGTTAAAGAGATGACATACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGATATTTTGCTTAATCCCCACAGGCTGGTTGTGGGGattaagc                                                                                                                                    <  2:754258/150‑5 (MQ=255)
              tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                                                                                    <  2:1511445/150‑1 (MQ=255)
              tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                                                                                    <  2:119811/150‑1 (MQ=255)
              tgtgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAt                                                                                                                                    <  1:680883/150‑1 (MQ=255)
                 ggTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTc                                                                                                                                 <  2:642843/150‑1 (MQ=255)
                  gTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCt                                                                                                                                >  1:1148459/1‑150 (MQ=255)
                  gTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCt                                                                                                                                >  2:1068060/1‑150 (MQ=255)
                   ccggcggAATGCGCGCGTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCTCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCTCGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCATCCTGTGGGGTTTTTATTTCtg                                                                                                                               <  1:1065801/149‑1 (MQ=255)
                    cggcggAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgt                                                                                                                              >  1:1461665/1‑150 (MQ=255)
                         gAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTag                                                                                                                         <  2:1633041/150‑1 (MQ=255)
                          aaTGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCATCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTaga                                                                                                                        >  1:1033571/1‑150 (MQ=255)
                             gcgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagagag                                                                                                                     >  2:624643/1‑150 (MQ=255)
                             gcgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagagag                                                                                                                     >  2:1232330/1‑150 (MQ=255)
                               gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagtt                                                                                                                   >  1:804975/1‑150 (MQ=255)
                                  cTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATATTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgag                                                                                                                <  2:354875/150‑1 (MQ=255)
                                   ttCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                                                                               <  1:202707/150‑1 (MQ=255)
                                   ttCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                                                                               <  1:567006/150‑1 (MQ=255)
                                   ttCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgagt                                                                                                               <  1:670129/150‑1 (MQ=255)
                                     cTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                                                                             >  2:35597/1‑150 (MQ=255)
                                      tATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCa                                                                                                            >  2:1733426/1‑150 (MQ=255)
                                      tATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCa                                                                                                            >  2:507695/1‑150 (MQ=255)
                                         ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCTTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                         <  2:393147/150‑1 (MQ=255)
                                         ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                         >  2:433117/1‑150 (MQ=255)
                                         ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                         <  1:1532899/150‑1 (MQ=255)
                                          tataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAACCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                                                                                        >  1:1282261/1‑150 (MQ=255)
                                           ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGaa                                                                                                       <  2:1148459/150‑1 (MQ=255)
                                             aaCGCCCTGCCGCTGGAAGGCGTTAAAGCGCGGACAGACGTGATGGGTTAGTTAGCACGCCGTCACGGTTTATGGCGAATTTTTGGTTAATCCCCCCAGCCAGCCGGGGGGGGTTGTTTTTGGGTTGTTGAGTGGTGAGTTAATGGGAtg                                                                                                     >  2:351591/1‑149 (MQ=255)
                                             aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATc                                                                                                     <  1:705006/150‑1 (MQ=255)
                                              aCGCCATGCCGCTGGAAGGCGTTTACGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGGTAATGCCGAAAGTTTGCTTAATCCCCACAGCCAGCCTGTGGGGGTTTTATTTCAGTTGTAGAGAGTTCAGTTCAGCGAATcc                                                                                                    >  1:1210431/1‑150 (MQ=255)
                                              aCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATcc                                                                                                    <  1:1590231/150‑1 (MQ=255)
                                              aCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATcc                                                                                                    <  2:1185456/150‑1 (MQ=255)
                                               cGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATccc                                                                                                   >  2:1515881/1‑150 (MQ=255)
                                                gCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                  <  1:839827/150‑1 (MQ=255)
                                                 ccATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTg                                                                                                 >  2:936815/1‑150 (MQ=255)
                                                   aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGAc                                                                                               <  2:1715383/150‑1 (MQ=255)
                                                       cGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTa                                                                                           <  1:242789/150‑1 (MQ=255)
                                                         cTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCATCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACa                                                                                         <  1:1163604/150‑1 (MQ=255)
                                                           ggAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAAc                                                                                       >  1:368442/1‑150 (MQ=255)
                                                               ggCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGCCGTTACACCCCAt                                                                                   >  1:822843/1‑150 (MQ=255)
                                                                 cGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCg                                                                                 >  1:1690395/1‑150 (MQ=255)
                                                                 cGTTAAAGCGCTGACAGACTTAATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAATTTTTGCTTAATCCCCACAGCAAGAATGTGGGGTTTTGATTACTGTTGTAGGGAGTTGAGTTCATGGAATCCCTACCGTTACAACCCATct                                                                                 >  2:1119704/1‑149 (MQ=255)
                                                                  gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGc                                                                                >  1:854580/1‑150 (MQ=255)
                                                                  gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGc                                                                                >  1:942323/1‑150 (MQ=255)
                                                                   ttAAAGCGCTGACAGACTTCATGGTTGAGTGCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCt                                                                               <  1:1071069/150‑1 (MQ=255)
                                                                     aaaGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAAACCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCg                                                                             <  1:1321925/150‑1 (MQ=255)
                                                                       aGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgtg                                                                           >  1:1572892/1‑150 (MQ=255)
                                                                          gcTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCg                                                                        >  2:1599727/1‑150 (MQ=255)
                                                                               cAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGc                                                                   <  2:30394/150‑1 (MQ=255)
                                                                               cAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGc                                                                   >  2:493847/1‑150 (MQ=255)
                                                                                 gACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCAc                                                                 <  1:1137691/150‑1 (MQ=255)
                                                                                 gACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCAc                                                                 >  1:1236622/1‑150 (MQ=255)
                                                                                 gACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCAc                                                                 >  1:1484039/1‑150 (MQ=255)
                                                                                     tCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTAtt                                                             <  2:1461665/150‑1 (MQ=255)
                                                                                      cATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTa                                                            <  1:917685/150‑1 (MQ=255)
                                                                                            tGAGTTCGAACGCCGTCACGGTTAATGCCGTAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGc                                                      <  1:42015/150‑1 (MQ=255)
                                                                                             gAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGcc                                                     <  2:1467023/150‑1 (MQ=255)
                                                                                               gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCg                                                   >  1:1081260/1‑150 (MQ=255)
                                                                                               gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCg                                                   >  1:372532/1‑150 (MQ=255)
                                                                                                  cGAACGCCGTCACGGTTAATGCGGAATTTTTGCTTAATCCCCACCGCCAGCCTGTGGGGATTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGTCTCCCTGACGTTACAACCCATCGCTGGTGTCGATGGCACTATTAATCTGCCCGGtt                                                >  2:83329/1‑150 (MQ=255)
                                                                                                  cGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGtt                                                >  1:185406/1‑150 (MQ=255)
                                                                                                  cGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTc                                               <  1:746552/150‑1 (MQ=255)
                                                                                                     aCGCCGTCTCGGTTAATGCGGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACTACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCa                                             <  2:1365533/150‑1 (MQ=255)
                                                                                                       gCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAag                                           <  1:1568621/150‑1 (MQ=255)
                                                                                                         cGTCACGGTTAAAGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAagag                                         <  2:42872/150‑1 (MQ=255)
                                                                                                            cACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTATTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGCCGTTACAACCAATAGCTCGTGTCTAGGGGCCTATTAATCTTCCCGGTACCAAGAGCGt                                      >  2:1277418/1‑150 (MQ=255)
                                                                                                                gTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCt                                  <  2:1265113/150‑1 (MQ=255)
                                                                                                                gTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCt                                  <  2:1294895/150‑1 (MQ=255)
                                                                                                                gTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCt                                  >  1:1294895/1‑150 (MQ=255)
                                                                                                                gTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCt                                  <  1:1630618/150‑1 (MQ=255)
                                                                                                                       ccGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACcgcg                           <  1:624643/150‑1 (MQ=255)
                                                                                                                          aaaTTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCtt                        <  1:1513810/150‑1 (MQ=255)
                                                                                                                            aTTTTGCTTAATCCCCACAGCCATCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCCTCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTa                      <  1:236105/150‑1 (MQ=255)
                                                                                                                             ttttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAt                     <  2:1571769/150‑1 (MQ=255)
                                                                                                                             agttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCATGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTAt                     <  2:1010025/148‑1 (MQ=255)
                                                                                                                             aattGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCATGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTan                     <  2:1016373/148‑2 (MQ=255)
                                                                                                                               ttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTAAAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCGCGGTTCCAAGAGAGTTTCTAACCGCGCGTTTTTg                   >  1:720911/1‑150 (MQ=255)
                                                                                                                                 gCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCt                 >  1:1452761/1‑150 (MQ=255)
                                                                                                                                 ataaaaaccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCTTTTCTAAAAGCGCTTTATTGCt                 >  1:1553462/8‑150 (MQ=255)
                                                                                                                                  cTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                >  1:351976/1‑150 (MQ=255)
                                                                                                                                  cTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                >  2:469164/1‑150 (MQ=255)
                                                                                                                                   aaaaaccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTgg               >  2:543963/6‑150 (MQ=255)
                                                                                                                                     aaaccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcg             >  2:371846/4‑150 (MQ=255)
                                                                                                                                     aaaccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTAGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAAACGCCCTTTAATGCTggcg             >  1:1598736/4‑150 (MQ=255)
                                                                                                                                     aaTCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcg             >  2:1016866/1‑150 (MQ=255)
                                                                                                                                       tCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGACTCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGATTCTAACCGCGCTTTATTGCTggcggc           >  1:847546/1‑150 (MQ=255)
                                                                                                                                         cccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt         >  1:1691966/1‑150 (MQ=255)
                                                                                                                                          ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAtt        <  2:804975/150‑1 (MQ=255)
                                                                                                                                           cacaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGCGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTa       <  1:103172/150‑1 (MQ=255)
                                                                                                                                            acaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAg      <  1:1068060/150‑1 (MQ=255)
                                                                                                                                             ccgccagccTGTGGGGTTTTTATATCTGGTGTAGAGCGTGGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGGCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGc     <  1:310564/148‑1 (MQ=255)
                                                                                                                                             cagccagcCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCTTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGc     <  1:472042/150‑1 (MQ=255)
                                                                                                                                              agccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGca    <  1:433117/150‑1 (MQ=255)
                                                                                                                                               gccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGcac   >  2:906218/1‑150 (MQ=255)
                                                                                                                                                ccagccTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGcaca  <  2:854580/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACA  >  NC_000913/958625‑958918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: