Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 963,764 G→A 8.6% intergenic (+96/‑64) rpsA → / → ihfB 30S ribosomal subunit protein S1/integration host factor (IHF), DNA‑binding protein, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913963,7640GA 8.6% 339.3 / 15.0 117intergenic (+96/‑64)rpsA/ihfB30S ribosomal subunit protein S1/integration host factor (IHF), DNA‑binding protein, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (52/54);  new base A (4/6);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-02

Only 100 of 117 total aligned reads displayed.

CTTCTCCAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAGTAAAAGAGA  >  NC_000913/963617‑963912
                                                                                                                                                   |                                                                                                                                                    
cTTCTCCAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGgc                                                                                                                                                    >  1:1581744/1‑150 (MQ=255)
   ctcCAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGCCAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGgccgc                                                                                                                                                 >  1:311318/1‑150 (MQ=255)
     ccAACCACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCGTCGGGATTTTTATTCCGAAGATTGTTGAGTTTACTGGACAGATTGCAGGTTTCGTCCTGGAATCACGCACTAAGCGCGCCTACGGCCGccc                                                                                                                                               >  2:481831/1‑150 (MQ=255)
     ccAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTTAAGGCGAGTAATTCTCTGACTCTTCGGGTTTTTTAATCCGAAGTTTGTTGAGGTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGccc                                                                                                                                               <  1:537939/150‑1 (MQ=255)
     ccAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGccc                                                                                                                                               >  2:425151/1‑150 (MQ=255)
     ccAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGccc                                                                                                                                               >  1:1435834/1‑150 (MQ=255)
      caacaaCGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCt                                                                                                                                              <  2:1027161/150‑1 (MQ=255)
       aacaacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCtt                                                                                                                                             <  1:75838/150‑1 (MQ=255)
       aacaacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCtt                                                                                                                                             <  1:1509111/150‑1 (MQ=255)
         caacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTag                                                                                                                                           <  2:721819/150‑2 (MQ=255)
          aacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCCGTAGCCGCCCTTagt                                                                                                                                          <  2:1588984/150‑3 (MQ=255)
          aacGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCACGTTTCGTCCTGTAATCAAGCACTAAGGGCGTCCGTAGCCGCCCTTagt                                                                                                                                          <  1:1105278/150‑3 (MQ=255)
            cGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCa                                                                                                                                        <  1:695680/150‑1 (MQ=255)
                aTGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgc                                                                                                                                    <  1:465787/150‑1 (MQ=255)
                 tGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgca                                                                                                                                   <  1:724300/150‑1 (MQ=255)
                   gCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCTAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCAGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgcagc                                                                                                                                 >  1:339509/1‑150 (MQ=255)
                   gCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCATCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTGTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgcagc                                                                                                                                 <  1:886049/150‑1 (MQ=255)
                    cTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATgcagca                                                                                                                                >  2:150640/1‑150 (MQ=255)
                        aGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAAcag                                                                                                                            >  2:802781/1‑150 (MQ=255)
                         gCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAAcagc                                                                                                                           >  2:123538/1‑150 (MQ=255)
                          cTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAAcagca                                                                                                                          >  2:1359253/1‑150 (MQ=255)
                          cTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAAcagca                                                                                                                          >  2:906362/1‑150 (MQ=255)
                            ttCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACagcagc                                                                                                                        <  2:1694569/150‑1 (MQ=255)
                            ttCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACagcagc                                                                                                                        <  2:906330/150‑1 (MQ=255)
                                aaGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCt                                                                                                                    >  1:1200932/1‑150 (MQ=255)
                                aaGCAGCAAACGGCGAGTAATACTCTGACTCTTCGGGATTTTAATTCCGAATTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCt                                                                                                                    <  2:1443189/150‑1 (MQ=255)
                                     gcTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAAtt                                                                                                               <  1:115045/150‑1 (MQ=255)
                                         aaGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGcc                                                                                                           <  1:304699/150‑1 (MQ=255)
                                            gCGAGTACTTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGGTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGCAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTACCttt                                                                                                        <  2:263151/150‑1 (MQ=255)
                                             cGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTa                                                                                                       <  1:1702476/150‑1 (MQ=255)
                                               aGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCTTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAg                                                                                                     <  1:1672070/150‑1 (MQ=255)
                                               aGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAg                                                                                                     <  2:1097122/150‑1 (MQ=255)
                                                    ttCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAAcc                                                                                                <  2:1435834/150‑1 (MQ=255)
                                                    ttCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAAcc                                                                                                <  1:885493/150‑1 (MQ=255)
                                                        ctGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTATTCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCggag                                                                                            >  1:337141/1‑150 (MQ=255)
                                                        ctGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCggag                                                                                            >  1:1379611/1‑150 (MQ=255)
                                                        ctGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCggag                                                                                            <  1:601871/150‑1 (MQ=255)
                                                         tGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCggagg                                                                                           <  1:511391/150‑1 (MQ=255)
                                                          gACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGATTACTTGACAGATTGAAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCAGCCCTAAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTAAAGGAACCggagga                                                                                          >  1:1115058/1‑150 (MQ=255)
                                                            ctctTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAAt                                                                                        <  2:665053/150‑1 (MQ=255)
                                                                 cGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGa                                                                                   >  1:763624/1‑150 (MQ=255)
                                                                  gggATTTTTATTCTGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCCTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTTATGAACCGGAGGAATCATGAc                                                                                  <  1:749201/150‑1 (MQ=255)
                                                                     aTTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCaa                                                                               >  1:717878/1‑150 (MQ=255)
                                                                      tttttATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAg                                                                              <  1:950748/150‑1 (MQ=255)
                                                                        tttATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGTAACCGGAGGAATCATGACCAAGta                                                                            >  2:96044/1‑149 (MQ=255)
                                                                          tATTCTGAATTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCATTGCAGCTACAGCAGCCGCTTAATTTGCCTTTAATTAACCGGAGTAATCATGACCATGTCAg                                                                          <  2:869929/150‑1 (MQ=255)
                                                                                gAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGa                                                                    <  1:123538/150‑1 (MQ=255)
                                                                                gAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGCGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAATGAACCGGAGGAATCATGACCAAGTCAGAATTGa                                                                    <  2:1644684/150‑1 (MQ=255)
                                                                                 aaGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGAt                                                                   <  1:496346/150‑1 (MQ=255)
                                                                                  aGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATa                                                                  >  2:1525174/1‑150 (MQ=255)
                                                                                  aGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATa                                                                  <  1:449063/150‑1 (MQ=255)
                                                                                  aGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATa                                                                  >  2:575757/1‑150 (MQ=255)
                                                                                       tttcaGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTCATAGAAAg                                                             >  1:49686/5‑150 (MQ=255)
                                                                                          gAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACt                                                          >  2:1691117/1‑150 (MQ=255)
                                                                                            gTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTg                                                        <  2:311318/150‑1 (MQ=255)
                                                                                            gTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATATGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACtat                                                        >  2:397025/1‑148 (MQ=255)
                                                                                              ttACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGAAACAGCATACGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGcc                                                      <  2:1300209/150‑1 (MQ=255)
                                                                                               tACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCa                                                     >  1:543645/1‑150 (MQ=255)
                                                                                                   tGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCa                                                 >  1:682113/1‑150 (MQ=255)
                                                                                                    gACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAg                                                >  2:405221/1‑150 (MQ=255)
                                                                                                     aCAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGc                                               <  2:813262/150‑1 (MQ=255)
                                                                                                     aCAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGGCAGAATTGATAGAAAGACTTGCCACCCAGc                                               <  1:1261246/150‑1 (MQ=255)
                                                                                                        gATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAAt                                            >  1:228078/1‑150 (MQ=255)
                                                                                                            gCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCCAGACAGAATTGATAGAAAGACTTGCCACCCAGCAATCGca                                        >  1:765/1‑150 (MQ=255)
                                                                                                            gCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACACAGCAATCGca                                        >  1:256654/1‑150 (MQ=255)
                                                                                                                  ttCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTcc                                  >  2:823216/1‑150 (MQ=255)
                                                                                                                    cGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGGCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCg                                <  1:1261486/150‑1 (MQ=255)
                                                                                                                     gTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGACCCGGAGGGATCATGACCAAGTCAGAATTGATAGAAAGACTGGCCACCCAGCAATCGCACATTCCCGc                               >  2:267103/1‑150 (MQ=255)
                                                                                                                     gTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGCAAGACTTGCCACCCAGCAATCGCACATTCCCGc                               >  1:735738/1‑150 (MQ=255)
                                                                                                                      tcatGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGcc                              <  1:397118/147‑1 (MQ=255)
                                                                                                                       ccTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCa                             <  1:906362/150‑1 (MQ=255)
                                                                                                                         tGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAg                           <  2:517037/150‑1 (MQ=255)
                                                                                                                           tAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGCCTTGCCACCCAGCAATCGCACATTCCCGCCAAGAc                         >  1:1678504/1‑150 (MQ=255)
                                                                                                                           tAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGAc                         <  1:1409972/150‑1 (MQ=255)
                                                                                                                             aTCAAGCACTAAGGGCGGCTACGGCCGCCCTTAAACAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACATGCCACCCCGCAAACGCACATTCCCGCCAAGACgg                       >  2:949942/1‑150 (MQ=255)
                                                                                                                               cAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGtt                     <  2:229335/150‑1 (MQ=255)
                                                                                                                               cAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGtt                     >  2:806767/1‑150 (MQ=255)
                                                                                                                               cAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGtt                     >  1:1113454/1‑150 (MQ=255)
                                                                                                                                attgattAAGGGCGGCCGTAGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTg                    >  2:527011/7‑150 (MQ=255)
                                                                                                                                aaGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCCCCCAGCAATCGCACATTCCCGCCAAGACGGTTg                    >  2:571155/1‑150 (MQ=255)
                                                                                                                                aaGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTg                    >  1:957169/1‑150 (MQ=255)
                                                                                                                                  gCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGaa                  >  1:1491999/1‑150 (MQ=255)
                                                                                                                                   gattAAGGGCGGCCGTAGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCCGCAATCGCACATTCCCGCCAAGACGGTTGAAg                 >  1:1002656/4‑150 (MQ=255)
                                                                                                                                   gattAAGGGCGGCCGTAGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATCACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCCAGACGGTTGAAg                 >  2:343005/4‑150 (MQ=255)
                                                                                                                                   cACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAg                 <  1:802781/150‑1 (MQ=255)
                                                                                                                                   cACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAg                 <  2:1379611/150‑1 (MQ=255)
                                                                                                                                    aCTAAGGGCGGCTACTGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCGTTAAGGAACCGGAGGAATCATGACCAAGTCAGATTTGATAGAAAGACTTGCCACCCAGTAATCGCACATTCCCGCCAAGACGGGTGAAGa                <  1:309502/150‑1 (MQ=255)
                                                                                                                                    aCTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCTATTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGa                <  1:96044/150‑1 (MQ=255)
                                                                                                                                       aaGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGCCGGTTGAAGATGc             >  1:852408/1‑150 (MQ=255)
                                                                                                                                       aaGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGc             >  2:415629/1‑150 (MQ=255)
                                                                                                                                       aaGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGc             <  2:1200932/150‑1 (MQ=255)
                                                                                                                                       aaGGGCGGCCGTAGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCAACCAGCAATCGCACATTCCCGCCAAGACGGTGGAAGATGc             >  1:1530272/1‑150 (MQ=255)
                                                                                                                                         gggCGGCGACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAg           <  2:365817/150‑1 (MQ=255)
                                                                                                                                           gcggcTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGCCGGTTGAAGATGCAGTa         >  2:404650/1‑150 (MQ=255)
                                                                                                                                              gcTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGGTGAAGATGCAGTaaaa      >  1:563450/1‑150 (MQ=255)
                                                                                                                                               cTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAGTAAAag     <  2:894001/150‑1 (MQ=255)
                                                                                                                                                tACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAGTAAAaga    >  1:1037106/1‑150 (MQ=255)
                                                                                                                                                tACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGATTAATTTGCATTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAGTAAAaga    <  1:1359253/150‑1 (MQ=255)
                                                                                                                                                tACGGCCGCCCTTAATCAATGCAGAAACAGCCGCCGCTTAATTTGCCTGTAAGGAACCGGAGGAATCATGCCCAAGTCAGCATTGATAGAAAGACGTGCGCCCCGGCAATCGCACATTACCGCCAAGCCGGTTTCATATGAAGAAAAaga    >  2:297138/1‑150 (MQ=255)
                                                                                                                                                  cGGCCGCCCTTAATCAATGCAGCAACAGAAGCCGCTTAATTTGCCTTTAAGGAAACGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCATCAATCGCACAATACCGACAAGACGGGTGAAGATGAAGTAAAAtaga  >  1:1024301/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTTCTCCAACAACGCAATGGCTGAAGCTTTCAAAGCAGCTAAAGGCGAGTAATTCTCTGACTCTTCGGGATTTTTATTCCGAAGTTTGTTGAGTTTACTTGACAGATTGCAGGTTTCGTCCTGTAATCAAGCACTAAGGGCGGCTACGGCCGCCCTTAATCAATGCAGCAACAGCAGCCGCTTAATTTGCCTTTAAGGAACCGGAGGAATCATGACCAAGTCAGAATTGATAGAAAGACTTGCCACCCAGCAATCGCACATTCCCGCCAAGACGGTTGAAGATGCAGTAAAAGAGA  >  NC_000913/963617‑963912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: