Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,259 C→T 12.0% intergenic (+25/+18) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2590CT12.0% 319.6 / 23.7 119intergenic (+25/+18)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (46/55);  new base T (6/8);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAG  >  NC_000913/993110‑993408
                                                                                                                                                     |                                                                                                                                                     
tGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgc                                                                                                                                                       <  2:895646/150‑1 (MQ=255)
 gATTCGCCTGAAACTTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCAGGAACAGTTGAAAGGGCTGGAAAATCTCTATGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcg                                                                                                                                                      >  2:488001/1‑150 (MQ=255)
        cTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCAt                                                                                                                                               >  2:237379/1‑150 (MQ=255)
         tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAtt                                                                                                                                              <  2:1225475/150‑1 (MQ=255)
         tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAtt                                                                                                                                              <  2:10574/150‑1 (MQ=255)
             aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCggt                                                                                                                                          <  1:149094/150‑2 (MQ=255)
             aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCggt                                                                                                                                          <  1:62074/150‑2 (MQ=255)
             aCGTTACGAGGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                          <  1:1452829/150‑1 (MQ=255)
              cGTTACGATGCCAACCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCggtt                                                                                                                                         <  2:1687271/150‑3 (MQ=255)
                  aCGATGCCAAACGGCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAtt                                                                                                                                     <  1:313976/150‑1 (MQ=255)
                   cGATGCCAAACGTCATGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGTCTGGAAAATCTCTCTGGCGATCTGTACGAGAATATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAttt                                                                                                                                    <  2:825392/150‑1 (MQ=255)
                      tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                                 <  1:544083/150‑1 (MQ=255)
                      tGCCAAACATCAGGAGAAAATGCGCGCGGCGCTGGAACTGTTGAAAGTGCGGCAAAATCTCTCTGGCGATCTGTAGGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                                 <  2:1350413/150‑1 (MQ=255)
                           aaCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGt                                                                                                                            >  2:1466791/1‑150 (MQ=255)
                             cGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCATTAGCGCCGCTATTCGGGGAATTTACCCCGGttt                                                                                                                          >  1:843821/1‑150 (MQ=255)
                             cGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGttt                                                                                                                          >  1:1289712/1‑150 (MQ=255)
                               tctggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTTAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtc                                                                                                                        <  2:758034/147‑1 (MQ=255)
                                 aGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctc                                                                                                                      <  2:686071/150‑1 (MQ=255)
                                  ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                     <  2:968571/150‑1 (MQ=255)
                                   gagaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAg                                                                                                                    <  1:215748/150‑1 (MQ=255)
                                    agaAAATGCGCTCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAgg                                                                                                                   <  2:1718429/150‑1 (MQ=255)
                                     gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                  >  2:1426657/1‑150 (MQ=255)
                                     gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                  <  1:1366130/150‑1 (MQ=255)
                                      aaaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCg                                                                                                                 <  1:431894/150‑1 (MQ=255)
                                        aaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAg                                                                                                               >  1:688013/1‑150 (MQ=255)
                                         aTGCGCGCGGCGCTGGAACAGTTGATAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCAGTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGt                                                                                                              >  1:255927/1‑150 (MQ=255)
                                         aTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGt                                                                                                              <  2:1477639/150‑1 (MQ=255)
                                         aTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGt                                                                                                              <  1:1403926/150‑1 (MQ=255)
                                         aTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGt                                                                                                              <  1:632072/150‑1 (MQ=255)
                                         aTGCGCGCGGCGCGGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGt                                                                                                              <  1:318054/150‑1 (MQ=255)
                                           gcgcgcGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGttt                                                                                                            >  2:400073/1‑150 (MQ=255)
                                              cgcggcgcggGAACAGTTGAAAGGGCTGGAAACTCTCTCTGGCGATCTGTACGAGAAGAAAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                         <  2:272613/150‑1 (MQ=255)
                                              cgcgGCGCTGGAACAGTTTAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                         <  2:611567/150‑1 (MQ=255)
                                              cgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                         >  1:1681941/1‑150 (MQ=255)
                                              cgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                         <  2:1698278/150‑1 (MQ=255)
                                                   cgcTGGAACAGTTGTTAGGGCTTGAAAATCTCTCTGGCGATCTGTACTAGAAGATAACTAAAGCACTGGCTTGATAAATATCCGAATGGCGGCAATTGCGCCGCCTTTCGGGGAATTTAGCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                    <  1:1184687/150‑1 (MQ=255)
                                                       ggAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcaccac                                                                                                <  1:1431715/150‑1 (MQ=255)
                                                        gAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACg                                                                                               >  1:44551/1‑150 (MQ=255)
                                                             gTTGACAGGGCTGGAAATAGTCTCTTGCGTTCTGTACGAGAATATAACTAAAGCACTGTCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                          <  2:819551/150‑1 (MQ=255)
                                                             gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                          <  2:364295/150‑1 (MQ=255)
                                                                gAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCa                                                                                       >  2:476544/1‑150 (MQ=255)
                                                                 aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAc                                                                                      <  1:269239/150‑1 (MQ=255)
                                                                    ggtcTGGAAAATCTCTCAGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAAAGAGCCGACATTCTGGGAATATACACCTGTTTTCTCAGGCGAGTTTCACATACAACCCGTTGCATCACCCg                                                                                   >  2:697171/4‑150 (MQ=255)
                                                                         ggAAAATCTCTCTGGCGATCTGTACGTGAAGATAACTAAAGCCCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCa                                                                              <  1:925779/150‑1 (MQ=255)
                                                                             aaTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAacac                                                                          >  1:716240/1‑150 (MQ=255)
                                                                               tctctcTGGCGTTCTGTACGAGAATATAACTAAAGCACTTGCTTGATAAATAACCGAATGGCGGGAATAGCGCAGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCt                                                                        <  2:275060/150‑1 (MQ=255)
                                                                                 tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                                      <  2:352462/150‑1 (MQ=255)
                                                                                  ctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCg                                                                     >  1:1427878/1‑150 (MQ=255)
                                                                                     ttgCGATCTGTACGAGAAGATAACTAAAGCTATGGCTTGATAAATAACTGAATGGCGGCAATAGCGCAGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCtt                                                                  <  1:284099/148‑1 (MQ=255)
                                                                                           tCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCTGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCt                                                            >  2:1219070/1‑150 (MQ=255)
                                                                                            cTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTc                                                           <  2:831761/150‑1 (MQ=255)
                                                                                             tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGATGCAACACATCCGCTTCCAGCTcc                                                          >  2:767486/1‑150 (MQ=255)
                                                                                              gTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCg                                                         >  2:30024/1‑150 (MQ=255)
                                                                                                 cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                      <  2:990069/150‑1 (MQ=255)
                                                                                                 cGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCCAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTa                                                      <  2:257387/150‑1 (MQ=255)
                                                                                                   agaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAAt                                                    <  1:1097919/150‑1 (MQ=255)
                                                                                                    gaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                                   <  2:520406/150‑1 (MQ=255)
                                                                                                    gaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGAGCCGACATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                                   <  2:656561/150‑1 (MQ=255)
                                                                                                      agaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCCCCCGTTGCAACCCCTCCGCTTCCAGCTCCGCTAATCGc                                                 >  2:955738/1‑150 (MQ=255)
                                                                                                      agaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGc                                                 <  2:618330/150‑1 (MQ=255)
                                                                                                      agaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGc                                                 >  2:932442/1‑150 (MQ=255)
                                                                                                         tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCTGCTAATCGCAcc                                              <  1:1426657/150‑1 (MQ=255)
                                                                                                         tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcc                                              <  2:1021659/150‑1 (MQ=255)
                                                                                                            cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACCCCTCCGCTTCCAGCTCCGCTAATCGCACCGAc                                           >  2:360239/1‑150 (MQ=255)
                                                                                                            cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTTATCGCACCGAc                                           <  2:396489/150‑1 (MQ=255)
                                                                                                            cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAc                                           >  2:1703238/1‑150 (MQ=255)
                                                                                                            cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCACTAATCGCACCGAc                                           >  1:1359352/1‑150 (MQ=255)
                                                                                                              aaaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGcccc                                         >  1:402080/1‑150 (MQ=255)
                                                                                                              aaaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTAGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAccc                                         <  2:1288804/150‑1 (MQ=255)
                                                                                                               aaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCt                                        >  1:1027772/1‑150 (MQ=255)
                                                                                                                aGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGCCCCTa                                       >  1:664339/1‑150 (MQ=255)
                                                                                                                 gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                                      >  1:659917/1‑150 (MQ=255)
                                                                                                                       gCTTGATAAATAACGGAATGGCGGCAATAGAGACGGCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcg                                <  2:858749/150‑1 (MQ=255)
                                                                                                                           gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGa                            >  1:1117104/1‑150 (MQ=255)
                                                                                                                           gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGa                            >  2:1149200/1‑150 (MQ=255)
                                                                                                                           gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATAGCACCGCCCCTAATCGACGCGGa                            >  2:509198/1‑150 (MQ=255)
                                                                                                                           gATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATGTACCCCTGGTTTCTCAGGCGAGTTTCAGAGTCACCACGTTGGATCACCCGTTGCAACACCTCCGCTGCCAGCTCCGCTAATCGCTCCGCCCCTGATCGACGCggc                            >  1:116991/1‑149 (MQ=255)
                                                                                                                               aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                        <  2:1275128/150‑1 (MQ=255)
                                                                                                                               aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                        <  1:1227977/150‑1 (MQ=255)
                                                                                                                                ataaCCGAAAGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTACAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTACCAGCTCCGCTAATCGCACCGACCCTAAACGACGCGGACcggg                       >  2:745022/1‑150 (MQ=255)
                                                                                                                                 taaCCGAATGGCGGCTATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGCCCCTAATCGACGCGGACggggg                      >  1:1289414/1‑150 (MQ=255)
                                                                                                                                 taaCCGAATGGCGGCTATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggg                      <  2:4927/150‑1 (MQ=255)
                                                                                                                                 taaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggggg                      <  2:1669622/150‑1 (MQ=255)
                                                                                                                                  ccccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACGCCTGATTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGCACGGGTGa                     >  1:1553238/3‑150 (MQ=255)
                                                                                                                                   cccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCGAATCGGCGCGGACGGGGGat                    >  1:1630228/2‑150 (MQ=255)
                                                                                                                                    ccGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTAACCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCTTCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGata                   >  1:1141315/1‑150 (MQ=255)
                                                                                                                                    ccGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTTCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGata                   <  1:400073/150‑1 (MQ=255)
                                                                                                                                       aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc                >  2:456793/1‑150 (MQ=255)
                                                                                                                                        aTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                 >  2:313740/1‑132 (MQ=255)
                                                                                                                                        aTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                 <  1:313740/132‑1 (MQ=255)
                                                                                                                                        aTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                 >  2:1008713/1‑132 (MQ=255)
                                                                                                                                        aTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                 <  1:1008713/132‑1 (MQ=255)
                                                                                                                                         tGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCAc              >  1:1408325/1‑150 (MQ=255)
                                                                                                                                            cggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGCTTCACATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGt           <  1:1078661/150‑1 (MQ=255)
                                                                                                                                             ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTc          <  1:563897/150‑1 (MQ=255)
                                                                                                                                             ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTc          >  2:1406514/1‑150 (MQ=255)
                                                                                                                                              gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa         <  1:1516166/150‑1 (MQ=255)
                                                                                                                                              gcAAAAGCGCCGCAATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCACATTCACCCCGTTGCATCACCCGATGCAACACCTCCGCGGCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa         >  1:1053036/1‑150 (MQ=255)
                                                                                                                                                attAGCGCCGCGATTCGGGGAATTTACCCCTGTTTGCTCAGGCGAGTTTCAGATTCAGCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa       <  1:488001/148‑1 (MQ=255)
                                                                                                                                                     cgccgcCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAg  <  2:716240/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCAG  >  NC_000913/993110‑993408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: