Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,887 C→T 6.8% intergenic (+39/‑141) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8870CT 6.8% 304.7 / 8.0 104intergenic (+39/‑141)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base C (44/51);  new base T (5/2);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-02

Only 100 of 106 total aligned reads displayed.

AGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAACTG  >  NC_000913/87738‑88036
                                                                                                                                                     |                                                                                                                                                     
aGCGGTATGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAc                                                                                                                                                       >  1:296712/1‑150 (MQ=255)
  cGGTTAGGTTGATGCATTTGTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGATCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCTTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGt                                                                                                                                                     <  1:290655/150‑1 (MQ=255)
     tAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGGTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTgg                                                                                                                                                  <  2:1536803/150‑1 (MQ=255)
       aGCTTGATGCATTTTTAGCATCGATTCGCGAGGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                                                                                <  1:1420150/150‑1 (MQ=255)
         cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGTAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtg                                                                                                                                              <  1:1331612/150‑2 (MQ=255)
         cTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt                                                                                                                                              >  1:734905/1‑150 (MQ=255)
           tGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt                                                                                                                                            >  2:1632783/1‑150 (MQ=255)
            gATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttt                                                                                                                                           <  1:9304/150‑1 (MQ=255)
             aTGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGTAGCTTGCTCGCTCTGGGGTGGTCGGACTTTCGCGCTGCGATAAAATAATGAGTTGAGAAAGATCTCAATGCGCAAATTACAGCCCAACGTGACATGTTGGGCtgtaaa                                                                                                                                          <  1:1565275/150‑6 (MQ=255)
               gCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                                                                        <  2:556873/150‑1 (MQ=255)
               gCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTAGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAAAATGTCACGTTGGGCTTTTTTTg                                                                                                                                        >  2:580868/1‑150 (MQ=255)
                cATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGc                                                                                                                                       >  1:1711731/1‑150 (MQ=255)
                    tttAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaaa                                                                                                                                   >  2:1603246/1‑150 (MQ=255)
                       aGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCa                                                                                                                                >  1:1164519/1‑150 (MQ=255)
                         cATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                                                                              >  1:1558020/1‑150 (MQ=255)
                           tCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTATGGTGTGGTCTGACTTTCGCGCGTCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                                                                            <  1:859674/150‑1 (MQ=255)
                              aTTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGaa                                                                                                                         >  2:642894/1‑150 (MQ=255)
                                      tgtgGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAAt                                                                                                                 >  1:1628939/1‑150 (MQ=255)
                                       gtgGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATa                                                                                                                <  1:776750/150‑1 (MQ=255)
                                         ggCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaa                                                                                                              >  2:35453/1‑150 (MQ=255)
                                           cGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg                                                                                                            >  2:310007/1‑150 (MQ=255)
                                            gAAAAATGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGGTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                                                                           <  1:293717/150‑1 (MQ=255)
                                              aaaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                                                                         >  2:1021088/1‑150 (MQ=255)
                                              aaaTTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAg                                                                                                         <  1:493634/150‑1 (MQ=255)
                                                   gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                                    <  2:549081/150‑1 (MQ=255)
                                                   gtgGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                                                                    >  2:897851/1‑150 (MQ=255)
                                                        ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                                                                               >  1:807016/1‑150 (MQ=255)
                                                         gTTGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGtt                                                                                              <  2:852236/150‑1 (MQ=255)
                                                           tGCTCGCTCTGGTGTGGGCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTaa                                                                                            <  1:943306/150‑1 (MQ=255)
                                                            gctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAt                                                                                           >  2:558082/1‑150 (MQ=255)
                                                             ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAtt                                                                                          <  2:1558020/150‑1 (MQ=255)
                                                                gctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGGTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTc                                                                                       >  1:271580/1‑150 (MQ=255)
                                                                 ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCt                                                                                      >  2:1202751/1‑150 (MQ=255)
                                                                  tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAAGATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                                     <  2:1202584/150‑1 (MQ=255)
                                                                  tctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                                                                     <  2:868034/150‑1 (MQ=255)
                                                                          ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                                                                             <  2:1171598/150‑1 (MQ=255)
                                                                           gTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGAt                                                                            <  1:310007/150‑1 (MQ=255)
                                                                           gTCGGACTTACGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTAGGGCTTTATTTGCGAAATCAGTTGGAACCTGGAATAAAAGCAGTTCACGCAGTTAAATTTCTGCGCTTAGAt                                                                            >  1:548166/1‑150 (MQ=255)
                                                                              gtaCTTACGCGCGGCGATAAATTAATGCGTTGAGATTGATCTCTATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCATTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                         <  2:1422810/148‑1 (MQ=255)
                                                                              ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                                                                         <  1:1345588/150‑1 (MQ=255)
                                                                               gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGAAGATAATTTTCTGCGCTTAGATGTTa                                                                        >  2:431500/1‑150 (MQ=255)
                                                                               gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGAGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                                                                        >  1:331334/1‑150 (MQ=255)
                                                                                   ttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACGTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGa                                                                    <  2:881139/150‑1 (MQ=255)
                                                                                     cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                                                                  <  1:851602/150‑1 (MQ=255)
                                                                                     cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                                                                  <  1:1680839/150‑1 (MQ=255)
                                                                                           gcgATAAAATAATGCGTTGAGTATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGGGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTTGATGTTAATGAATTTAAcc                                                            <  2:561950/150‑1 (MQ=255)
                                                                                            cgATTAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc                                                           <  2:1072340/150‑1 (MQ=255)
                                                                                              aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCAt                                                         >  1:707416/1‑150 (MQ=255)
                                                                                               tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATa                                                        >  2:1308255/1‑150 (MQ=255)
                                                                                                 aaaTAATGCGTTGAGAATGATCTCAATGCGCATTTTACAGCCCAAAATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAcc                                                      <  1:641109/150‑1 (MQ=255)
                                                                                                 aaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATAcc                                                      <  1:893486/150‑1 (MQ=255)
                                                                                                  aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCa                                                     <  2:67543/150‑1 (MQ=255)
                                                                                                  aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCa                                                     >  2:1246225/1‑150 (MQ=255)
                                                                                                   ataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACAAg                                                    >  1:521966/1‑150 (MQ=255)
                                                                                                     aatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTa                                                  >  1:373369/1‑150 (MQ=255)
                                                                                                           ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATgg                                            >  1:1081376/1‑150 (MQ=255)
                                                                                                             gagaATGATCTCAATGCGCAATTTACAGCACAACATGTCACGTTGGTCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCt                                          <  2:51826/150‑1 (MQ=255)
                                                                                                             gagaATGATCTAAATGCGCAATTTACAGCCCAACATTTGACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAATCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCt                                          <  2:129141/150‑1 (MQ=255)
                                                                                                             gagaATCATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTATGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCt                                          <  2:46501/150‑1 (MQ=255)
                                                                                                               taaTGATCTCAATGCGCAATTTACAGCCCAACCTGTCAAGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTTCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTAt                                        <  2:1210155/149‑1 (MQ=255)
                                                                                                               gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTAt                                        <  2:734905/150‑1 (MQ=255)
                                                                                                               gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCAAGTTGGGGTTTTTTTGCGAAATCAGTGGGCACCTGGAATAAAAGCAGGTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACaaagtcgat                                        >  2:894294/1‑143 (MQ=255)
                                                                                                                aaTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATg                                       <  1:1603246/150‑1 (MQ=255)
                                                                                                                 aTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATgg                                      <  1:200048/150‑1 (MQ=255)
                                                                                                                    aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttt                                   >  1:1601464/1‑150 (MQ=255)
                                                                                                                     tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtttt                                  >  1:1398406/1‑150 (MQ=255)
                                                                                                                     tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGtttt                                  <  2:1511801/150‑1 (MQ=255)
                                                                                                                      ctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGttttt                                 >  2:428703/1‑150 (MQ=255)
                                                                                                                        cAATGCGCAATTTACAGCCCACCATGTCACGTGGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTGAATGAATTTAACCCATACCAGTACAAGGGCTAGGGTGTTTaa                               >  2:180501/1‑149 (MQ=255)
                                                                                                                        cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                               <  1:700515/150‑1 (MQ=255)
                                                                                                                        cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                               >  2:15217/1‑150 (MQ=255)
                                                                                                                        cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAc                               >  2:180498/1‑150 (MQ=255)
                                                                                                                         aaTGCTCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACa                              <  1:721558/150‑1 (MQ=255)
                                                                                                                         aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACa                              <  1:726983/150‑1 (MQ=255)
                                                                                                                            gcgcAATTTACAGCCCAACATGTCACGTTGGGGGTTTATTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGGTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACGAGTACAATGGCTATGGTTTTTACAttt                           >  1:1065698/1‑150 (MQ=255)
                                                                                                                            gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAttt                           <  2:1372506/150‑1 (MQ=255)
                                                                                                                            gagcAATTTTCAGCCCAACATTTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAACTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACAttt                           <  1:1134367/148‑1 (MQ=255)
                                                                                                                               cAATTTACAGCCCAACATGTCACGTTTGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTAc                        <  1:1005950/150‑1 (MQ=255)
                                                                                                                               cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCATTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTATGCGATTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTaa                        >  2:1543278/1‑149 (MQ=255)
                                                                                                                               cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTAc                        <  1:1426233/150‑1 (MQ=255)
                                                                                                                               cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTAc                        <  1:1586024/150‑1 (MQ=255)
                                                                                                                                aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACg                       <  1:642894/150‑1 (MQ=255)
                                                                                                                                 aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGc                      <  2:1164519/150‑1 (MQ=255)
                                                                                                                                    aaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAg                   >  2:459095/4‑150 (MQ=255)
                                                                                                                                    aaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAg                   >  2:762534/4‑150 (MQ=255)
                                                                                                                                     aaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAgg                  >  1:501899/3‑150 (MQ=255)
                                                                                                                                      cAGCCCAACATGTCACGTTTGGCTTTTTTTGCTAAATCAGTTGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg                 <  1:461404/150‑1 (MQ=255)
                                                                                                                                      aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAggg                 >  1:1447185/2‑150 (MQ=255)
                                                                                                                                        gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTAAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGc               >  1:292434/1‑150 (MQ=255)
                                                                                                                                          ccAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCaa             >  2:1380324/1‑150 (MQ=255)
                                                                                                                                           cAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAAt            >  1:407545/1‑150 (MQ=255)
                                                                                                                                                tGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGa       <  1:520104/150‑1 (MQ=255)
                                                                                                                                                tGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGa       >  1:1510611/1‑150 (MQ=255)
                                                                                                                                                 gTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTTGATGTTAATGAATTTAACACATACAAGTAAAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGaa      >  2:142194/1‑150 (MQ=255)
                                                                                                                                                 gTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGaa      >  2:641157/1‑150 (MQ=255)
                                                                                                                                                   cACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAAc    >  1:992305/1‑150 (MQ=255)
                                                                                                                                                   cACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAAc    >  2:1335457/1‑150 (MQ=255)
                                                                                                                                                   cACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAAc    <  2:1367786/150‑1 (MQ=255)
                                                                                                                                                    aCGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAACt   >  1:802522/1‑150 (MQ=255)
                                                                                                                                                     cGTTGGGAGTTTTTTGCGAAATCAGTTGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAACTg  <  1:184029/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGATGTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTACATTTTACGCAAGGGGCAATTGTGAAACTG  >  NC_000913/87738‑88036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: