Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,026,541 T→C 9.2% intergenic (+16/+16) helD → / ← mgsA DNA helicase IV/methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,026,5410TC 9.2% 235.1 / 11.8 86intergenic (+16/+16)helD/mgsADNA helicase IV/methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base T (38/40);  new base C (4/4);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

AAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGTCG  >  NC_000913/1026392‑1026690
                                                                                                                                                     |                                                                                                                                                       
aagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAATGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGttt                                                                                                                                                         >  1:819399/1‑150 (MQ=255)
  gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                       <  2:1119851/150‑1 (MQ=255)
  gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                       <  2:287149/150‑1 (MQ=255)
   aaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGcc                                                                                                                                                      <  2:518228/150‑1 (MQ=255)
   aaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGcc                                                                                                                                                      <  2:1429277/150‑1 (MQ=255)
   aaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGcc                                                                                                                                                      <  1:1233383/150‑1 (MQ=255)
        cggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtg                                                                                                                                                 <  1:1667944/150‑1 (MQ=255)
         ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAGTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgt                                                                                                                                                <  2:867965/150‑1 (MQ=255)
             taTGTAGGTGGCGCTGAGCCGGGCAGGCCTTCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGTAAATTCTGGATGTGCCGGTGGCGAGATAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttct                                                                                                                                            <  2:1660982/149‑5 (MQ=255)
                gTACGTGGCGCTGACCCGGGCTCGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttcttac                                                                                                                                         <  1:380987/150‑8 (MQ=255)
                 tACGTGGCGCTGACCCGTGCACGCCAGCGCGTAGGGGCACTGTTTAACAAAGAGCATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAACCCGTAAGCAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                        <  2:1147368/150‑1 (MQ=255)
                 tACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                        <  2:816370/150‑1 (MQ=255)
                   cGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAGTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTAc                                                                                                                                      >  1:667703/1‑150 (MQ=255)
                   cGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTAc                                                                                                                                      >  2:721448/1‑150 (MQ=255)
                   cGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTAc                                                                                                                                      <  2:1239397/150‑1 (MQ=255)
                        ggcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAg                                                                                                                                 <  2:1334883/149‑1 (MQ=255)
                        cggtGACGCGGGCACGCCATCGTGTATGGGCACTGTTTAACAAAGAGAATCGCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAg                                                                                                                                 <  2:116492/147‑1 (MQ=255)
                        cgcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAg                                                                                                                                 <  1:889042/150‑1 (MQ=255)
                        cgcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAg                                                                                                                                 >  1:365092/1‑150 (MQ=255)
                           tGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACg                                                                                                                              >  1:56090/1‑150 (MQ=255)
                              cccGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAACCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTc                                                                                                                           >  1:1316324/1‑150 (MQ=255)
                              cccGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTc                                                                                                                           >  1:661232/1‑150 (MQ=255)
                               ccGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTcc                                                                                                                          <  1:134359/150‑1 (MQ=255)
                                cGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAAAATTACTTCAGACGGTCcg                                                                                                                         >  2:200711/1‑150 (MQ=255)
                                  ggCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGAGGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCcgcg                                                                                                                       <  1:1668983/150‑1 (MQ=255)
                                         cATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCGGGATGTGCCGGTTGCGAGAAGACCGTAAGAAACAGGTGGCGATTGCCACCTGTGCAATATTACATCATACGGTCCGCGAGATAAc                                                                                                                >  2:313720/1‑150 (MQ=255)
                                            cGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCt                                                                                                             <  1:516851/150‑1 (MQ=255)
                                             gggTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTg                                                                                                            <  2:1378116/150‑1 (MQ=255)
                                                    ggCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCg                                                                                                   >  2:764084/1‑150 (MQ=255)
                                                     gCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCgg                                                                                                  <  2:1252856/150‑1 (MQ=255)
                                                      cACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCggg                                                                                                 >  1:1053358/1‑150 (MQ=255)
                                                       aCTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCgggg                                                                                                <  2:869614/150‑1 (MQ=255)
                                                          gTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATc                                                                                             >  1:1139461/1‑150 (MQ=255)
                                                            ttAAGAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAGATCggaagagc                                                                                            <  2:301713/150‑7 (MQ=255)
                                                            ttAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAg                                                                                           <  2:1629748/150‑1 (MQ=255)
                                                             tAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACTGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGa                                                                                          >  2:702745/1‑150 (MQ=255)
                                                              aaCAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGACGGTGGCGAGAAAACCGTAAGAACCAGGTGGCGTTTGCCACATGTGCCATATTACTTCAGACGCTCCGCGAGAAAACGCTGATCATCGCGGAGCAGaa                                                                                         >  1:279924/1‑150 (MQ=255)
                                                                 aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                      >  1:426487/1‑150 (MQ=255)
                                                                  aaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCAGCGAGATAACGCTGATAATCGTGGATCAGAatata                                                                                     >  1:1084248/1‑149 (MQ=255)
                                                                   agagAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCTCGATATAACGCTGATAATCGGGGCTCTGAATATCg                                                                                    >  1:148503/1‑150 (MQ=255)
                                                                   agagAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCg                                                                                    >  2:1677890/1‑150 (MQ=255)
                                                                    gagaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGa                                                                                   <  1:1426507/150‑1 (MQ=255)
                                                                    cagaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGa                                                                                   >  1:172908/2‑150 (MQ=255)
                                                                      gaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGAcc                                                                                 <  2:1478101/150‑1 (MQ=255)
                                                                      gaATCCCTCTACCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGAAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGAcc                                                                                 <  2:1688547/150‑1 (MQ=255)
                                                                           ccTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtc                                                                            >  2:808472/1‑150 (MQ=255)
                                                                            ctctCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtcg                                                                           >  2:456280/1‑150 (MQ=255)
                                                                              ctcCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGtt                                                                         <  2:1712658/150‑1 (MQ=255)
                                                                                cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGCATATCGACCGCGTCGTTGa                                                                       >  1:1089035/1‑150 (MQ=255)
                                                                                cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                       <  1:772805/150‑1 (MQ=255)
                                                                                       tgGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATgcgg                                                                <  1:738385/150‑1 (MQ=255)
                                                                                        ggAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTGGAAATgcggc                                                               >  1:666834/1‑150 (MQ=255)
                                                                                           aaTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGCTCAGAATATCGACCGCGTCGTTGAAATGCGGCGAc                                                            >  1:1159039/1‑150 (MQ=255)
                                                                                            aTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTAAGACGGTCCGCGAGATTACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGATGAAAAGCGGCGACt                                                           >  2:635820/1‑150 (MQ=255)
                                                                                              aCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTgg                                                         <  1:759662/150‑1 (MQ=255)
                                                                                                tGAAAAATCTGGATGTTCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                       <  2:1476686/150‑1 (MQ=255)
                                                                                                tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                       >  1:1192972/1‑150 (MQ=255)
                                                                                                    aaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATg                                                   <  2:207735/150‑1 (MQ=255)
                                                                                                    aaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATg                                                   <  2:172908/150‑1 (MQ=255)
                                                                                                    aaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATg                                                   >  2:691435/1‑150 (MQ=255)
                                                                                                     aaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGa                                                  <  1:1430034/150‑1 (MQ=255)
                                                                                                      aTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGaa                                                 >  2:855050/1‑150 (MQ=255)
                                                                                                              gtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCg                                         >  1:592746/1‑150 (MQ=255)
                                                                                                              gtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCg                                         <  2:1316324/150‑1 (MQ=255)
                                                                                                               tgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGt                                        >  1:1384972/1‑150 (MQ=255)
                                                                                                                      ggCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACg                                 >  2:1541534/1‑150 (MQ=255)
                                                                                                                        cGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCAGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGt                                >  1:64102/1‑150 (MQ=255)
                                                                                                                        cGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACAGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGtt                               >  1:1228818/1‑150 (MQ=255)
                                                                                                                         gagaAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGAGCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtg                              >  1:333340/1‑150 (MQ=255)
                                                                                                                           gaAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGACCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGAGGAGACTGGATTATGAAGTCTGCAGTTGCCACCTtgct                            >  2:785513/1‑148 (MQ=255)
                                                                                                                            aaaaCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtggtg                           <  1:1709802/150‑1 (MQ=255)
                                                                                                                            aaaaCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtggtg                           <  2:64304/150‑1 (MQ=255)
                                                                                                                            aaaaCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtggtg                           <  1:862216/150‑1 (MQ=255)
                                                                                                                               aCCGTAAGAAACAGGTGGAGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCg                        <  2:968405/150‑1 (MQ=255)
                                                                                                                                  gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                     >  1:1139541/1‑150 (MQ=255)
                                                                                                                                  gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                     <  2:770107/150‑1 (MQ=255)
                                                                                                                                  atattgcaCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                     >  1:399042/8‑150 (MQ=255)
                                                                                                                                      gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCAGTTGCCACGTTGGTGGCGACCGGaa                 >  1:1603639/1‑150 (MQ=255)
                                                                                                                                      gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa                 <  2:1192972/150‑1 (MQ=255)
                                                                                                                                        caCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATg               >  1:1605825/2‑150 (MQ=255)
                                                                                                                                         aCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt              >  1:1326324/1‑150 (MQ=255)
                                                                                                                                         aCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt              >  2:381995/1‑150 (MQ=255)
                                                                                                                                          cAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGtt             >  2:565751/1‑150 (MQ=255)
                                                                                                                                           cggTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGGCCGCGAGATAACGCTGATAAGCGGGGAGCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGGCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTc            <  1:1054978/149‑1 (MQ=255)
                                                                                                                                            ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTcc           >  1:929887/1‑150 (MQ=255)
                                                                                                                                               ggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATa        <  1:721448/150‑1 (MQ=255)
                                                                                                                                                 cGTTTCCCACCTGTGCAATATTACTTCAGACCGTCCGCGAGATAACGCTGAAACTCGCGGACCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATAcc      <  1:1069076/150‑1 (MQ=255)
                                                                                                                                                     tGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCTGAATATCGACCGCGTCGTTGAAATGCGGCGAGTGGATTATGATGTCTTCCGTTGCCACGTTGGTGGCGTCCGGTATTTTACATACCGTCg  >  2:121638/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
AAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGTCG  >  NC_000913/1026392‑1026690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: