Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,052,264 A→G 9.7% intergenic (+24/+25) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,052,2640AG 9.7% 308.4 / 20.1 115intergenic (+24/+25)gnsA/yccMputative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base A (58/45);  new base G (6/5);  total (64/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

Only 100 of 115 total aligned reads displayed.

TATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAC  >  NC_000913/1052117‑1052408
                                                                                                                                                   |                                                                                                                                                
tATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACTGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAgg                                                                                                                                                >  2:1625207/1‑150 (MQ=255)
tATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAgg                                                                                                                                                <  1:1679870/150‑1 (MQ=255)
tATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAgg                                                                                                                                                <  1:785318/150‑1 (MQ=255)
 aTCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGt                                                                                                                                               <  1:216093/150‑1 (MQ=255)
  tCGCTCAAAAAATCGCCGAGCTGAACAAGAATACAGTGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTTATTTTGTTAACATCACCCCGTGCGAGGTg                                                                                                                                              <  2:1713388/150‑1 (MQ=255)
  tCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTg                                                                                                                                              <  1:564763/150‑1 (MQ=255)
     cgcAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATg                                                                                                                                           <  2:1460286/150‑1 (MQ=255)
     cgcAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATg                                                                                                                                           <  1:1055028/150‑1 (MQ=255)
      gcAAAAAATCGGCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTGGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGt                                                                                                                                          <  2:1358286/150‑1 (MQ=255)
       cAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGtt                                                                                                                                         <  2:678670/150‑1 (MQ=255)
       cAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGAGGtt                                                                                                                                         >  1:823249/1‑150 (MQ=255)
        aaaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTc                                                                                                                                        <  1:755415/150‑1 (MQ=255)
         aaaacTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTca                                                                                                                                       <  1:1112248/150‑2 (MQ=255)
         aaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGGGATGTTcc                                                                                                                                       <  1:853610/150‑1 (MQ=255)
           aaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGc                                                                                                                                     <  1:307940/150‑1 (MQ=255)
                gCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgat                                                                                                                                >  2:273949/1‑148 (MQ=255)
                 ccGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgttg                                                                                                                               <  1:908677/150‑1 (MQ=255)
                       tGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAAtt                                                                                                                         >  1:603271/1‑150 (MQ=255)
                        gAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTTTGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTAGTTGCTAAttt                                                                                                                        >  2:1089051/1‑150 (MQ=255)
                           cAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGCCCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCTCTTGTTGCTAATTTAGt                                                                                                                     >  2:887832/1‑150 (MQ=255)
                           cAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATTATGTCAAAATCAAAATAATGTCATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGt                                                                                                                     >  2:1187137/1‑150 (MQ=255)
                           cAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGt                                                                                                                     >  1:554156/1‑150 (MQ=255)
                            aaGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTg                                                                                                                    <  1:754067/150‑1 (MQ=255)
                            aaGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTg                                                                                                                    <  2:1464163/150‑1 (MQ=255)
                            aaGAATACAGGGAAAGAAGTCGCTGAAATTCGCTTCACCGCACGAGAAAAAATGCCCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTg                                                                                                                    <  1:827692/150‑1 (MQ=255)
                              gAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAc                                                                                                                  >  1:817549/1‑150 (MQ=255)
                              gAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAc                                                                                                                  <  2:1392847/150‑1 (MQ=255)
                               aaTACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAcc                                                                                                                 <  1:1104029/150‑1 (MQ=255)
                                   cAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                             <  1:1220732/150‑1 (MQ=255)
                                   cAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                             >  1:559606/1‑150 (MQ=255)
                                   aaGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACTATAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                             <  1:340559/149‑1 (MQ=255)
                                      ggAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCAtt                                                                                                          >  1:1437993/1‑150 (MQ=255)
                                           gaagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGgcgc                                                                                                     >  2:117653/1‑150 (MQ=255)
                                            aagGCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGCCCGGGCTGGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCt                                                                                                    <  1:1404794/150‑1 (MQ=255)
                                             agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATTATGTGATTTTGTGAACATCACCACGTGCGAGGTGATCTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                   >  2:603181/1‑150 (MQ=255)
                                             agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                   >  2:848169/1‑150 (MQ=255)
                                             agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCGTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                   >  2:1270570/1‑150 (MQ=255)
                                                ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                >  1:220682/1‑150 (MQ=255)
                                                  ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                              >  1:222579/1‑150 (MQ=255)
                                                  ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                              >  1:1277318/1‑150 (MQ=255)
                                                  ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                              >  1:88369/1‑150 (MQ=255)
                                                    gAAATTCGCTTCACCGCACGAGAAAATATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAAt                                                                                            <  1:1586894/150‑1 (MQ=255)
                                                         tCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGc                                                                                       <  1:1411997/150‑1 (MQ=255)
                                                          cGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCg                                                                                      <  2:424330/150‑1 (MQ=255)
                                                            cTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTc                                                                                    <  2:1516311/150‑1 (MQ=255)
                                                             ttCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAATTAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCg                                                                                   <  1:656358/150‑1 (MQ=255)
                                                                   gCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGGTAATTTAGTGACCAATCATTGGCGCTTGAGGAATTAAGCGTCGGTACaa                                                                             >  2:753362/1‑150 (MQ=255)
                                                                    cACGAGAAAAAATGACCGGGCTTGAAAGTAATGATGTCAAAATCAAAATAATGAGAATTTCTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCAATGCAGCTAGTGGAATTAAGAGTCGGTACaaa                                                                            >  2:521204/1‑149 (MQ=255)
                                                                        agaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCAGCTTGTTGCTAATTTAGTGACCAATCATTGGCACTTGAGGAATTAAGCGTCGGTACAATtcct                                                                        >  1:1686495/1‑150 (MQ=255)
                                                                         gaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctc                                                                       <  2:744230/150‑1 (MQ=255)
                                                                          aaaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctcc                                                                      <  1:361484/150‑1 (MQ=255)
                                                                          aaaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctcc                                                                      >  1:917558/1‑150 (MQ=255)
                                                                                  aCCGGGCGTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGCCCAATCATTGGCGCTTGTGGAATTAAGCTGCGGTACAATTCCTCCGGCACCgg                                                              >  1:163859/1‑150 (MQ=255)
                                                                                     gggCTTGTAAGTTTTGATGTCAAAATCAAAATTATGTTATTTTGTGAACAGCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCt                                                           <  1:151555/150‑1 (MQ=255)
                                                                                       gCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCttt                                                         <  1:1345994/150‑1 (MQ=255)
                                                                                        ctggAAAGTTATGATGTCAAAATGATAATAATGTGATTTTGTTAACATCACCCCGTGCGTGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAAGCATTGGCGCTTGTGGAATTCAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTg                                                        <  1:209601/147‑1 (MQ=255)
                                                                                        cTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTg                                                        >  2:1678405/1‑150 (MQ=255)
                                                                                        cTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTACTCCGGCACCGGGCTTTg                                                        >  2:1671863/1‑150 (MQ=255)
                                                                                          tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGcc                                                      >  1:738896/1‑150 (MQ=255)
                                                                                          tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGcc                                                      <  2:1109129/150‑1 (MQ=255)
                                                                                          tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAACAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGcc                                                      >  1:1704324/1‑150 (MQ=255)
                                                                                           gAAAGTTATGATGACAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAAGTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCa                                                     <  1:1260548/150‑1 (MQ=255)
                                                                                            aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCAt                                                    >  2:1239198/1‑150 (MQ=255)
                                                                                                 tATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTcc                                               <  2:63946/150‑1 (MQ=255)
                                                                                                 tATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACACCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTcc                                               >  1:651904/1‑150 (MQ=255)
                                                                                                  atgatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTccc                                              >  1:1332266/1‑150 (MQ=255)
                                                                                                    gatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGc                                            <  1:73052/150‑1 (MQ=255)
                                                                                                     atgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCa                                           >  2:314443/1‑150 (MQ=255)
                                                                                                      tgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATCCTCCCGCAt                                          >  2:87108/1‑150 (MQ=255)
                                                                                                       gTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATa                                         >  1:714348/1‑150 (MQ=255)
                                                                                                       cacaaaatcacattATTTTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATa                                         >  2:581656/3‑150 (MQ=255)
                                                                                                         caaaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTACCGCTAGTTGCTAATTTAGTGACCATACATTGGCGCTTGTGGAATTAAGCTACGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCAAACa                                       >  2:121918/1‑150 (MQ=255)
                                                                                                            aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                    >  1:1686604/1‑150 (MQ=255)
                                                                                                            aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGGGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                    <  1:1313989/150‑1 (MQ=255)
                                                                                                               caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGaa                                                                                             >  2:1494013/1‑90 (MQ=255)
                                                                                                               caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGaa                                                                                             <  1:1494013/90‑1 (MQ=255)
                                                                                                               aaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGt                                 <  1:1706507/149‑1 (MQ=255)
                                                                                                                     aatGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCaa                           <  2:1726258/150‑1 (MQ=255)
                                                                                                                       tgtgATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATc                         <  2:742259/150‑1 (MQ=255)
                                                                                                                       tgtgATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATc                         <  1:520835/150‑1 (MQ=255)
                                                                                                                       tgtgATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTACTGACCAATCATTGGAGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTACCCAATc                         >  2:467587/1‑150 (MQ=255)
                                                                                                                               gcggAACATCCCCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTCAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTTCGTAACCAATCACCGCaaa                 >  1:1189332/4‑150 (MQ=255)
                                                                                                                                gtgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTTACCAATCACCGCAAAc                >  2:184365/1‑150 (MQ=255)
                                                                                                                                gtgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAAc                >  1:807261/1‑150 (MQ=255)
                                                                                                                                gtgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAAc                >  2:276753/1‑150 (MQ=255)
                                                                                                                                 tgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACa               >  1:186857/1‑150 (MQ=255)
                                                                                                                                 gtaaCATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACa               >  1:527212/3‑150 (MQ=255)
                                                                                                                                 ggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACa               >  1:965431/2‑150 (MQ=255)
                                                                                                                                 ggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGAGATTGTGGAATTAAGCGTCGGTACAAATCCTCCGGCACCGCGCTTTGACATACTCCCGCATACATTGCGTAACCAATCACCGGAAACa               >  2:1513383/2‑150 (MQ=255)
                                                                                                                                   aaCATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATTCATTGCGTAACCAATCACCGCAAACATa             >  2:659534/1‑150 (MQ=255)
                                                                                                                                   aaCATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa             >  1:998278/1‑150 (MQ=255)
                                                                                                                                    aCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATaa            <  1:1187137/150‑1 (MQ=255)
                                                                                                                                    aCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATaa            >  1:1176560/1‑150 (MQ=255)
                                                                                                                                      aTCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATc          >  1:1281522/1‑150 (MQ=255)
                                                                                                                                       tCACCCCGTGCAAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTACCCAATCACCGCAAACATAATcc         >  2:191093/1‑150 (MQ=255)
                                                                                                                                        cACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGTAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTATCCAATCACCGCAAACATAATccc        >  2:1140387/1‑150 (MQ=255)
                                                                                                                                         aCCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTACCCAATCACGGCACGCATAATcccc       >  1:116551/1‑150 (MQ=255)
                                                                                                                                          ccccGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCa      <  2:603271/150‑1 (MQ=255)
                                                                                                                                              gTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAc  <  2:559606/150‑1 (MQ=255)
                                                                                                                                              gTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAc  >  1:828359/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAC  >  NC_000913/1052117‑1052408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: