BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,064,160	T→C	6.9%	L42P (CTG→CCG) ‡	yccE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,160	0	T	C	6.9%	389.1 / 13.6	131	L42P (CTG→CCG) ‡	yccE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (67/55); new base C (5/4); total (72/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 131 total aligned reads displayed.

TATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACT  >  NC_000913/1064012‑1064308

tattaAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTg                                                                                                                                                     >  2:315000/1‑150 (MQ=255)

tattaAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCAATGCAAATAATTATTTGAAACATGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTAAATCAAGCTTTTTGAAAACACAATg                                                                                                                                                     >  1:247030/1‑150 (MQ=255)

 attaAATTTTATAGACTCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTgg                                                                                                                                                    <  1:657222/150‑1 (MQ=255)

  ttaAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgt                                                                                                                                                   >  1:826588/1‑150 (MQ=255)

   taAATTTTATAGACGCTATATATGTGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                  <  2:377469/150‑1 (MQ=255)

   taAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                  >  2:920170/1‑150 (MQ=255)

   taAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                  >  2:1509241/1‑150 (MQ=255)

   taAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                  >  1:431281/1‑150 (MQ=255)

   taAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                  >  2:128617/1‑150 (MQ=255)

    aaaTTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgt                                                                                                                                                 >  1:793499/1‑150 (MQ=255)

     aaTTTTATAGACGCTATATATTGGTAGTAATATACATGGATTTAGTTGCACTGAAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtg                                                                                                                                                <  1:1659169/150‑1 (MQ=255)

      aTTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                               >  2:1504638/1‑150 (MQ=255)

        tttATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                                                                                                                                             <  2:431708/150‑1 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                           <  1:466462/150‑1 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttc                                                                                                                                           <  2:1475728/150‑2 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttc                                                                                                                                           <  1:780801/150‑2 (MQ=255)

             aGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttcaaa                                                                                                                                        <  1:952150/150‑5 (MQ=255)

                cGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttcaaaaag                                                                                                                                     <  1:29921/150‑8 (MQ=255)

                          gggTAGTAATATACATGGAATTAGTTGCATTGCAAATAATTATTTGTAACAGGCCTGGAACGATATAATAAATGAGTACGAAAAAAATCATACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGa                                                                                                                           <  1:1582817/150‑1 (MQ=255)

                            gtagtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAAc                                                                                                                         <  2:502851/150‑1 (MQ=255)

                             tagtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACATGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGTTGTGTTTAATGCGGTTATACAATGAACt                                                                                                                        >  1:1449587/1‑150 (MQ=255)

                              agtaATATACATGTAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACTCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACta                                                                                                                       <  1:240502/150‑2 (MQ=255)

                               ttaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCa                                                                                                                      <  2:826588/149‑1 (MQ=255)

                                    ataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgt                                                                                                                 >  1:329187/1‑150 (MQ=255)

                                     taCATTGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCATGGAACGATATAAAAAATTAGTACGAAAAAAATCAAACATATTCAATCACGCTGTTTGAAAACACACTGTTGTTTTTTATGCTTTTATACAATGAACTAATACgta                                                                                                                >  2:1707861/1‑150 (MQ=255)

                                      aCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAATCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaa                                                                                                               >  1:217385/1‑150 (MQ=255)

                                       caaataaTTATTTGCAGTGCAACTAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaa                                                                                                              >  2:359307/6‑150 (MQ=255)

                                         tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACTAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagt                                                                                                            <  1:1155828/150‑1 (MQ=255)

                                          ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagta                                                                                                           <  2:1083008/150‑1 (MQ=255)

                                           gAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaa                                                                                                          >  2:1475425/1‑150 (MQ=255)

                                            aaTTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaaa                                                                                                         <  1:1502268/150‑1 (MQ=255)

                                             aTTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGTACTCAGACGTAAAGTAAAt                                                                                                        <  2:431281/150‑1 (MQ=255)

                                               tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGa                                                                                                      <  2:969234/150‑1 (MQ=255)

                                               tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGa                                                                                                      <  2:1174704/150‑1 (MQ=255)

                                               tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGa                                                                                                      <  2:1170939/150‑1 (MQ=255)

                                                aGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGaa                                                                                                     >  1:1039578/1‑150 (MQ=255)

                                                 gTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAag                                                                                                    >  2:541788/1‑150 (MQ=255)

                                                 gTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAag                                                                                                    <  1:1394191/150‑1 (MQ=255)

                                                   tGCACTTCAAATAAATATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTAAGAAAAAAATCAAACTTATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAagag                                                                                                  <  1:665768/150‑1 (MQ=255)

                                                   tGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGATTTTTTAAAACACACTGTTGTGTTTTATGAGTTTATACAATGAACTCATAAGTAAAGTAAATGAatag                                                                                                  >  1:231634/1‑150 (MQ=255)

                                                    gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAgg                                                                                                 <  2:971649/150‑1 (MQ=255)

                                                      aCTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGAt                                                                                               >  1:1391431/1‑150 (MQ=255)

                                                      aCTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGAt                                                                                               >  2:1355957/1‑150 (MQ=255)

                                                            aataatTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCa                                                                                         <  1:228721/150‑1 (MQ=255)

                                                              taatTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCTATg                                                                                       >  2:1670596/1‑150 (MQ=255)

                                                                   attTGAAACAGGTCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTAATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                  >  2:738244/1‑150 (MQ=255)

                                                                   attTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                  >  1:1277224/1‑150 (MQ=255)

                                                                   attTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                  >  2:619891/1‑150 (MQ=255)

                                                                         aaCAGGCCTGTAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAAtgtg                                                                            <  2:290022/150‑1 (MQ=255)

                                                                           cAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGaa                                                                          >  1:1687050/1‑150 (MQ=255)

                                                                            aGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                         <  2:1580697/150‑1 (MQ=255)

                                                                             ggCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAta                                                                        >  1:989874/1‑149 (MQ=255)

                                                                             ggCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATc                                                                        <  2:357801/150‑1 (MQ=255)

                                                                              gCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCa                                                                       <  1:31954/150‑1 (MQ=255)

                                                                                 tGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTa                                                                    <  2:685993/150‑1 (MQ=255)

                                                                                     aCGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaa                                                                >  2:1260244/1‑150 (MQ=255)

                                                                                          ataAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGaa                                                           >  2:1308651/1‑150 (MQ=255)

                                                                                           taAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCTGTTATACAAAGAACTCAGACGTAAAGTAAATTAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAt                                                          >  1:598371/1‑150 (MQ=255)

                                                                                            aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAtt                                                         >  2:573404/1‑150 (MQ=255)

                                                                                            aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAtt                                                         <  2:568124/150‑1 (MQ=255)

                                                                                            aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAtt                                                         <  1:315000/150‑1 (MQ=255)

                                                                                             aaataTGATTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTTTTTTATGCGTTTATAAAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAttt                                                        <  2:528660/150‑1 (MQ=255)

                                                                                             aaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAttt                                                        >  1:868500/1‑150 (MQ=255)

                                                                                                aaTGAGTACGAAAAAAATAAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATTAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAg                                                     <  1:328868/150‑1 (MQ=255)

                                                                                                     gTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                >  2:1097204/1‑150 (MQ=255)

                                                                                                     gTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                <  1:1462486/150‑1 (MQ=255)

                                                                                                        cGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                             <  2:446336/150‑1 (MQ=255)

                                                                                                        cGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                             >  2:391057/1‑150 (MQ=255)

                                                                                                        cGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                             >  1:872154/1‑150 (MQ=255)

                                                                                                        cGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGGGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                             <  1:252288/150‑1 (MQ=255)

                                                                                                         gAAAAAAATCAAACATATTCAATCACGCTTTTTGTAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                            <  1:1280706/150‑1 (MQ=255)

                                                                                                         gAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                            <  2:489104/150‑1 (MQ=255)

                                                                                                          aaaaaaaTCAAACATATTCAATCACTCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATTAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGa                                           <  1:1057773/150‑1 (MQ=255)

                                                                                                          aaaaaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGa                                           <  1:1063101/150‑1 (MQ=255)

                                                                                                              aaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatga                                       >  2:380021/1‑150 (MQ=255)

                                                                                                              aaaTCAAACATATTCAATCACGATTTTTGTAAACACACTTGTGTGTTTTATGCGGTTATAAAATGAACTCTGACGTAAAGTAAATTAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatga                                       <  2:1449587/150‑1 (MQ=255)

                                                                                                               aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgat                                      >  1:1670409/1‑150 (MQ=255)

                                                                                                                 tCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataa                                    <  1:38073/150‑1 (MQ=255)

                                                                                                                 tCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataa                                    <  2:329187/150‑1 (MQ=255)

                                                                                                                  cAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataat                                   <  2:115331/150‑1 (MQ=255)

                                                                                                                       atatTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCt                              >  1:975788/1‑150 (MQ=255)

                                                                                                                            cAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                         >  2:185880/1‑150 (MQ=255)

                                                                                                                              aTCTCGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGTCGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCatta                       <  2:326317/150‑1 (MQ=255)

                                                                                                                                cACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATAGATCattatc                     <  2:395863/150‑2 (MQ=255)

                                                                                                                                 aCGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTAt                            >  2:1486252/1‑142 (MQ=255)

                                                                                                                                    cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATg                 <  1:1481664/150‑1 (MQ=255)

                                                                                                                                    cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTATGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATTGAGAATGTTAATGATCTATCAttttttatt                 >  1:291788/1‑149 (MQ=255)

                                                                                                                                     tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATga                <  1:1690605/150‑1 (MQ=255)

                                                                                                                                      ttttGAAAACACTCTGTTTTGTTTTATGCGTTTATACATTTATCTCATAAGTAAATTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATgag               <  1:273302/150‑1 (MQ=255)

                                                                                                                                      cgcataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATAACTAGAAAAAGAATATGAGGAAATGCAGAATGATAATGATCTATCATTATTTATgag               >  1:1089702/6‑150 (MQ=255)

                                                                                                                                       gcataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATgaga              >  1:41128/5‑150 (MQ=255)

                                                                                                                                        cataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGaa             >  1:240632/4‑150 (MQ=255)

                                                                                                                                         tGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAat            >  1:777247/1‑150 (MQ=255)

                                                                                                                                            aaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATAtt         >  2:1720557/1‑150 (MQ=255)

                                                                                                                                             aaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTg        >  1:380342/1‑150 (MQ=255)

                                                                                                                                                 cacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGATTTTGAGGAAATGCAGAATGATAATGATCTATCTATATTTATGAGAATATTGAGTa    >  2:1074240/1‑150 (MQ=255)

                                                                                                                                                 cacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGAGTa    >  1:383848/1‑150 (MQ=255)

                                                                                                                                                  acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTAc   <  1:871323/150‑1 (MQ=255)

                                                                                                                                                  acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTAc   <  1:1355957/150‑1 (MQ=255)

                                                                                                                                                   cTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACt  >  2:1664126/1‑150 (MQ=255)

TATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACT  >  NC_000913/1064012‑1064308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`