Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,107,660 T→C 5.8% W436R (TGG→CGG) ‡ clsC → stationary phase cardiolipin synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,107,6600TC 5.8% 358.8 / 9.6 120W436R (TGG→CGG) ‡clsCstationary phase cardiolipin synthase 3
Reads supporting (aligned to +/- strand):  ref base T (56/56);  new base C (5/2);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 123 total aligned reads displayed.

ATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCT  >  NC_000913/1107511‑1107809
                                                                                                                                                     |                                                                                                                                                     
aTTTCGATCCGCGTTCAACATTGCTCAATACTGAACTGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACt                                                                                                                                                       <  1:327313/150‑1 (MQ=255)
aTTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACt                                                                                                                                                       <  1:1519020/150‑1 (MQ=255)
 tttCGATCCGCGTTCAACATTGCTCAATACTGAACTGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTg                                                                                                                                                      <  1:349907/150‑1 (MQ=255)
    cGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGt                                                                                                                                                   <  1:1249492/150‑1 (MQ=255)
    cGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGGGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGt                                                                                                                                                   <  2:21449/150‑1 (MQ=255)
     gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                  >  1:17613/1‑150 (MQ=255)
     gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                  >  2:440024/1‑150 (MQ=255)
      aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                 <  2:382733/150‑1 (MQ=255)
      aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                 <  2:199609/150‑1 (MQ=255)
           ccttCAACATTGCTCAATACTGAAAGGGGCTGCGGGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTAGGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGt                                                                                                                                            <  2:1162592/148‑1 (MQ=255)
            gTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACCCAGTTGATccgt                                                                                                                                           <  2:1028600/150‑4 (MQ=255)
            gTTCAACATTGCTAATTACTGAAATGGGCGTCGGGATCGAGAGCGAAACGCTGGCTCAGTTAATTGATACTCGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACCCAGTTGATccgt                                                                                                                                           <  1:1044542/150‑4 (MQ=255)
               cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATg                                                                                                                                        <  1:1065445/150‑1 (MQ=255)
               cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGc                                                                                                                                       >  2:1473919/1‑150 (MQ=255)
                         cAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGAACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAaagaaaga                                                                                                                              <  1:936172/150‑1 (MQ=255)
                            tACTTAAATGGTATTCCGGATATAGAGGGAAACGCTGTCACAGTTAATTGATAAACGCTATATTCAGAGACAGTTTGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGat                                                                                                                           <  2:157367/150‑1 (MQ=255)
                              cTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGatta                                                                                                                         >  1:1732195/1‑150 (MQ=255)
                                gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                       >  2:1435337/1‑150 (MQ=255)
                                gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                       >  2:363247/1‑150 (MQ=255)
                                gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                       >  1:1720381/1‑150 (MQ=255)
                                   aTGGGCTTCGTTATAGAGAGCGAAACGCTGGCACAGTTAATTGATTAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATtctc                                                                                                                    <  1:1470164/150‑1 (MQ=255)
                                   aTGGGCATCGTTATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGTAAGAGATTATtctc                                                                                                                    <  1:308117/150‑1 (MQ=255)
                                    tGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCAAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCa                                                                                                                   <  2:280182/150‑1 (MQ=255)
                                      ggCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGGCTGGACAGGTGGGGCCGGATCAACTGGGGTGATCGTCATGCAAAGAAAGAGATTATTCTCaaa                                                                                                                 >  2:1676254/1‑150 (MQ=255)
                                          tCGTGATAGAGAGCGAAACGCTGGCAAAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGTTGCGTCCTGGCAGCTCCGTCTGGACATGTGGGGACTTATCAACTGGGTTGATCGTCATGCAAAGATAGAGATTATTCTCAAAAAAg                                                                                                             <  1:783414/150‑1 (MQ=255)
                                          gcGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAg                                                                                                             <  1:1652647/149‑1 (MQ=255)
                                             tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGCTCGTCATGCAAAGAAAGAGATTATTCTAAAAAAAGAAc                                                                                                          >  2:1568848/1‑150 (MQ=255)
                                             tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                          >  1:1707180/1‑150 (MQ=255)
                                             tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                          <  2:198580/150‑1 (MQ=255)
                                             tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                          >  1:1170547/1‑150 (MQ=255)
                                              gATAGAGAGCGAAACGCTGGCACAGGTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAcc                                                                                                         <  2:1521077/150‑1 (MQ=255)
                                                tAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCg                                                                                                       <  2:17613/150‑1 (MQ=255)
                                                 agagagCGAAACGCTGGCACAGTGAATTGATACACGCTTTATTCAGAGCCATTATGAAGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGc                                                                                                      <  2:452533/150‑1 (MQ=255)
                                                  gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                     <  2:455521/150‑1 (MQ=255)
                                                  gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                     >  1:875684/1‑150 (MQ=255)
                                                   agagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcca                                                                                                    <  2:726739/150‑1 (MQ=255)
                                                      gCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGccacca                                                                                                 >  1:889828/1‑150 (MQ=255)
                                                       cGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAg                                                                                                >  2:1190273/1‑150 (MQ=255)
                                                            cGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCt                                                                                           <  1:28256/150‑1 (MQ=255)
                                                              cTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTgg                                                                                         <  1:68819/150‑1 (MQ=255)
                                                               tGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGa                                                                                        <  1:335820/150‑1 (MQ=255)
                                                                ggCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGaa                                                                                       >  1:1603080/1‑150 (MQ=255)
                                                                 gCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAg                                                                                      <  2:34699/150‑1 (MQ=255)
                                                                  cacaGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGc                                                                                     >  2:904694/1‑150 (MQ=255)
                                                                    caGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCgg                                                                                   >  1:181110/1‑150 (MQ=255)
                                                                     aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                  >  1:549933/1‑150 (MQ=255)
                                                                     aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                  <  1:113313/150‑1 (MQ=255)
                                                                       ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGtt                                                                                <  1:1717321/150‑1 (MQ=255)
                                                                       ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGtt                                                                                <  1:1463990/150‑1 (MQ=255)
                                                                         aaTTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCTTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGAGTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTAt                                                                              <  1:260684/150‑1 (MQ=255)
                                                                          aTTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCGGGAAGCGGGGTATg                                                                             >  2:342224/1‑150 (MQ=255)
                                                                           ttGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATgg                                                                            <  2:1650389/150‑1 (MQ=255)
                                                                            tGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGt                                                                           >  2:545741/1‑150 (MQ=255)
                                                                             gATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGATTCTGGAAGCGGGTTATGGTc                                                                          >  1:189439/1‑150 (MQ=255)
                                                                              aTAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCa                                                                         >  2:1166998/1‑150 (MQ=255)
                                                                                    gcgtgaTTCAGAGCCAGGATGATGCGGCCTGGCAGCTCCGTCTGGACCGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTgg                                                                   <  1:163070/145‑1 (MQ=255)
                                                                                         aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                              >  1:631029/1‑150 (MQ=255)
                                                                                         aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                              >  2:1386046/1‑150 (MQ=255)
                                                                                             agagCCATTATGATGCGGCCTGGAAGCTCCTTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATTGTCAGACTGGCGTCGatat                                                          <  1:203401/150‑1 (MQ=255)
                                                                                              gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCTATAtt                                                         >  1:320380/1‑150 (MQ=255)
                                                                                              gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATAtt                                                         >  1:45249/1‑150 (MQ=255)
                                                                                                 ccAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCATACTGGCGTCGATATTGcc                                                      <  2:1634258/150‑1 (MQ=255)
                                                                                                      atgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTgg                                                 >  2:1477301/1‑150 (MQ=255)
                                                                                                      atgacgCGGCCTGGCAGCTCCGTTTGGACAGGGGGGGACGGCTCAACGCGGTTGATCGTCATGCAAAGAAAGAGCTTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATAGCCCGTgg                                                 <  2:102084/150‑1 (MQ=255)
                                                                                                       tgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCCCCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGa                                                >  1:197567/1‑150 (MQ=255)
                                                                                                           gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGCAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTTGAATgg                                            >  2:1177607/1‑150 (MQ=255)
                                                                                                           gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATgg                                            <  2:1603080/150‑1 (MQ=255)
                                                                                                            cGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGt                                           <  1:1435337/150‑1 (MQ=255)
                                                                                                              gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTa                                         >  2:1018036/1‑150 (MQ=255)
                                                                                                                cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                       >  1:293903/1‑150 (MQ=255)
                                                                                                                cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                       >  1:106340/1‑150 (MQ=255)
                                                                                                                 ggctggCTCCTTCTGGACAGGTGGGGACGGATCTACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGATGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTg                                      <  2:723356/145‑1 (MQ=255)
                                                                                                                     aGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTaaa                                  >  2:508817/1‑150 (MQ=255)
                                                                                                                      gCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAAt                                 <  2:432529/150‑1 (MQ=255)
                                                                                                                        tCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAAtgt                               >  1:297896/1‑150 (MQ=255)
                                                                                                                             cTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTATGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCtt                          >  1:1218049/1‑150 (MQ=255)
                                                                                                                                 aCAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACg                      <  1:958269/150‑1 (MQ=255)
                                                                                                                                       ggggACGGATCTACTTGGTTGATCGTGATGCAACGAAAGAGATTATTCTCAAAAAAGAACCCGCAACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                <  2:1080999/150‑1 (MQ=255)
                                                                                                                                       ggggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                >  2:869412/1‑150 (MQ=255)
                                                                                                                                        catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt               >  1:1361179/7‑150 (MQ=255)
                                                                                                                                        catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt               >  2:905482/7‑150 (MQ=255)
                                                                                                                                         ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg              <  2:1720381/150‑1 (MQ=255)
                                                                                                                                         ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg              <  1:1018036/150‑1 (MQ=255)
                                                                                                                                         atgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg              >  1:648406/6‑150 (MQ=255)
                                                                                                                                         atgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg              >  1:546939/6‑150 (MQ=255)
                                                                                                                                          gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc             <  1:540718/150‑1 (MQ=255)
                                                                                                                                          gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc             >  2:386894/1‑150 (MQ=255)
                                                                                                                                          gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc             >  2:164753/1‑150 (MQ=255)
                                                                                                                                            cGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCtt           <  1:1110541/150‑1 (MQ=255)
                                                                                                                                              gATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATTGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTa         >  2:331266/1‑150 (MQ=255)
                                                                                                                                              gATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTa         >  1:657509/1‑150 (MQ=255)
                                                                                                                                               aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt        <  1:386894/150‑1 (MQ=255)
                                                                                                                                               aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt        >  1:600087/1‑150 (MQ=255)
                                                                                                                                                 cAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCg      <  1:423696/150‑1 (MQ=255)
                                                                                                                                                 cAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCg      <  2:744679/150‑1 (MQ=255)
                                                                                                                                                  aaCTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAATAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGAGATTGCCCGTGGAATGGTTATTGTAAATGTGCCTTAACGGGCAGGTGATTTaactt     >  1:448576/1‑146 (MQ=255)
                                                                                                                                                    cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc   <  2:305436/150‑1 (MQ=255)
                                                                                                                                                    cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGGCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc   <  1:1558064/150‑1 (MQ=255)
                                                                                                                                                    cTGGGGTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc   <  2:875684/150‑1 (MQ=255)
                                                                                                                                                     tGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtct  <  2:16655/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCT  >  NC_000913/1107511‑1107809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: