Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,107,661 G→C 6.5% W436S (TGG→TCG) ‡ clsC → stationary phase cardiolipin synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,107,6610GC 6.5% 373.4 / 12.9 120W436S (TGG→TCG) ‡clsCstationary phase cardiolipin synthase 3
Reads supporting (aligned to +/- strand):  ref base G (56/55);  new base C (5/3);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 123 total aligned reads displayed.

TTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTC  >  NC_000913/1107512‑1107810
                                                                                                                                                     |                                                                                                                                                     
tttCGATCCGCGTTCAACATTGCTCAATACTGAACTGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTg                                                                                                                                                       <  1:349907/150‑1 (MQ=255)
   cGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGGGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGt                                                                                                                                                    <  2:21449/150‑1 (MQ=255)
    gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                   >  1:17613/1‑150 (MQ=255)
    gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                   >  2:440024/1‑150 (MQ=255)
     aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                  <  2:199609/150‑1 (MQ=255)
     aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                  <  2:382733/150‑1 (MQ=255)
          ccttCAACATTGCTCAATACTGAAAGGGGCTGCGGGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTAGGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGt                                                                                                                                             <  2:1162592/148‑1 (MQ=255)
           gTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACCCAGTTGATccgt                                                                                                                                            <  2:1028600/150‑4 (MQ=255)
           gTTCAACATTGCTAATTACTGAAATGGGCGTCGGGATCGAGAGCGAAACGCTGGCTCAGTTAATTGATACTCGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACCCAGTTGATccgt                                                                                                                                            <  1:1044542/150‑4 (MQ=255)
              cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATg                                                                                                                                         <  1:1065445/150‑1 (MQ=255)
              cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGc                                                                                                                                        >  2:1473919/1‑150 (MQ=255)
                        cAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGAACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAaagaaaga                                                                                                                               <  1:936172/150‑1 (MQ=255)
                           tACTTAAATGGTATTCCGGATATAGAGGGAAACGCTGTCACAGTTAATTGATAAACGCTATATTCAGAGACAGTTTGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGat                                                                                                                            <  2:157367/150‑1 (MQ=255)
                               gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  2:363247/1‑150 (MQ=255)
                               gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  2:1435337/1‑150 (MQ=255)
                               gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  1:1720381/1‑150 (MQ=255)
                                  aTGGGCATCGTTATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGTAAGAGATTATtctc                                                                                                                     <  1:308117/150‑1 (MQ=255)
                                   tGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCAAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCa                                                                                                                    <  2:280182/150‑1 (MQ=255)
                                     ggCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGGCTGGACAGGTGGGGCCGGATCAACTGGGGTGATCGTCATGCAAAGAAAGAGATTATTCTCaaa                                                                                                                  >  2:1676254/1‑150 (MQ=255)
                                         tCGTGATAGAGAGCGAAACGCTGGCAAAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGTTGCGTCCTGGCAGCTCCGTCTGGACATGTGGGGACTTATCAACTGGGTTGATCGTCATGCAAAGATAGAGATTATTCTCAAAAAAg                                                                                                              <  1:783414/150‑1 (MQ=255)
                                            tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGCTCGTCATGCAAAGAAAGAGATTATTCTAAAAAAAGAAc                                                                                                           >  2:1568848/1‑150 (MQ=255)
                                            tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           >  1:1707180/1‑150 (MQ=255)
                                            tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           <  2:198580/150‑1 (MQ=255)
                                            tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           >  1:1170547/1‑150 (MQ=255)
                                             gATAGAGAGCGAAACGCTGGCACAGGTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAcc                                                                                                          <  2:1521077/150‑1 (MQ=255)
                                               tAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCg                                                                                                        <  2:17613/150‑1 (MQ=255)
                                                agagagCGAAACGCTGGCACAGTGAATTGATACACGCTTTATTCAGAGCCATTATGAAGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGc                                                                                                       <  2:452533/150‑1 (MQ=255)
                                                 gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                      <  2:455521/150‑1 (MQ=255)
                                                 gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                      >  1:875684/1‑150 (MQ=255)
                                                  agagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcca                                                                                                     <  2:726739/150‑1 (MQ=255)
                                                     gCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGccacca                                                                                                  >  1:889828/1‑150 (MQ=255)
                                                      cGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAg                                                                                                 >  1:16655/1‑150 (MQ=255)
                                                      cGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAg                                                                                                 >  2:1190273/1‑150 (MQ=255)
                                                           cGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCt                                                                                            <  1:28256/150‑1 (MQ=255)
                                                             cTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTgg                                                                                          <  1:68819/150‑1 (MQ=255)
                                                              tGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGa                                                                                         <  1:335820/150‑1 (MQ=255)
                                                               ggCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGaa                                                                                        >  1:1603080/1‑150 (MQ=255)
                                                                gCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAg                                                                                       <  2:34699/150‑1 (MQ=255)
                                                                 cacaGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGc                                                                                      >  2:904694/1‑150 (MQ=255)
                                                                   caGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCgg                                                                                    >  1:181110/1‑150 (MQ=255)
                                                                    aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                   <  1:113313/150‑1 (MQ=255)
                                                                    aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                   >  1:549933/1‑150 (MQ=255)
                                                                      ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGtt                                                                                 <  1:1717321/150‑1 (MQ=255)
                                                                      ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGtt                                                                                 <  1:1463990/150‑1 (MQ=255)
                                                                        aaTTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCTTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGAGTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTAt                                                                               <  1:260684/150‑1 (MQ=255)
                                                                         aTTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCGGGAAGCGGGGTATg                                                                              >  2:342224/1‑150 (MQ=255)
                                                                          ttGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATgg                                                                             <  2:1650389/150‑1 (MQ=255)
                                                                          ttGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATgg                                                                             <  1:1733108/150‑1 (MQ=255)
                                                                           tGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGt                                                                            >  2:545741/1‑150 (MQ=255)
                                                                            gATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGATTCTGGAAGCGGGTTATGGTc                                                                           >  1:189439/1‑150 (MQ=255)
                                                                             aTAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCa                                                                          >  2:1166998/1‑150 (MQ=255)
                                                                                   gcgtgaTTCAGAGCCAGGATGATGCGGCCTGGCAGCTCCGTCTGGACCGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTgg                                                                    <  1:163070/145‑1 (MQ=255)
                                                                                        aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                               >  1:631029/1‑150 (MQ=255)
                                                                                        aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                               >  2:1386046/1‑150 (MQ=255)
                                                                                             gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCTATAtt                                                          >  1:320380/1‑150 (MQ=255)
                                                                                             gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATAtt                                                          >  1:1283091/1‑150 (MQ=255)
                                                                                             gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATAtt                                                          >  1:45249/1‑150 (MQ=255)
                                                                                                ccAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCATACTGGCGTCGATATTGcc                                                       <  2:1634258/150‑1 (MQ=255)
                                                                                                     atgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTgg                                                  >  2:1477301/1‑150 (MQ=255)
                                                                                                     atgacgCGGCCTGGCAGCTCCGTTTGGACAGGGGGGGACGGCTCAACGCGGTTGATCGTCATGCAAAGAAAGAGCTTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATAGCCCGTgg                                                  <  2:102084/150‑1 (MQ=255)
                                                                                                      tgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCCCCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGa                                                 >  1:197567/1‑150 (MQ=255)
                                                                                                          gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGCAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTTGAATgg                                             >  2:1177607/1‑150 (MQ=255)
                                                                                                          gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATgg                                             <  2:1603080/150‑1 (MQ=255)
                                                                                                           cGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGt                                            <  1:1532735/150‑1 (MQ=255)
                                                                                                           cGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGt                                            <  1:1435337/150‑1 (MQ=255)
                                                                                                             gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTa                                          >  2:1018036/1‑150 (MQ=255)
                                                                                                               cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                        >  1:106340/1‑150 (MQ=255)
                                                                                                               cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                        >  1:293903/1‑150 (MQ=255)
                                                                                                                ggctggCTCCTTCTGGACAGGTGGGGACGGATCTACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGATGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTg                                       <  2:723356/145‑1 (MQ=255)
                                                                                                                    aGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTaaa                                   >  2:508817/1‑150 (MQ=255)
                                                                                                                     gCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAAt                                  <  2:432529/150‑1 (MQ=255)
                                                                                                                            cTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTATGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCtt                           >  1:1218049/1‑150 (MQ=255)
                                                                                                                              ggACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTaa                         >  1:305436/1‑150 (MQ=255)
                                                                                                                                aCAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACg                       <  1:958269/150‑1 (MQ=255)
                                                                                                                                      ggggACGGATCTACTTGGTTGATCGTGATGCAACGAAAGAGATTATTCTCAAAAAAGAACCCGCAACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                 <  2:1080999/150‑1 (MQ=255)
                                                                                                                                      ggggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                 >  2:869412/1‑150 (MQ=255)
                                                                                                                                       catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt                >  2:905482/7‑150 (MQ=255)
                                                                                                                                       catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt                >  1:1361179/7‑150 (MQ=255)
                                                                                                                                        ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               <  1:1018036/150‑1 (MQ=255)
                                                                                                                                        ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               <  2:1720381/150‑1 (MQ=255)
                                                                                                                                        atgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               >  1:546939/6‑150 (MQ=255)
                                                                                                                                        atgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               >  1:648406/6‑150 (MQ=255)
                                                                                                                                         gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              >  2:164753/1‑150 (MQ=255)
                                                                                                                                         gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              <  1:540718/150‑1 (MQ=255)
                                                                                                                                         gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              >  2:386894/1‑150 (MQ=255)
                                                                                                                                           cGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCtt            <  1:1110541/150‑1 (MQ=255)
                                                                                                                                             gATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATTGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTa          >  2:331266/1‑150 (MQ=255)
                                                                                                                                             gATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTa          >  1:657509/1‑150 (MQ=255)
                                                                                                                                              aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt         >  1:600087/1‑150 (MQ=255)
                                                                                                                                              aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt         <  1:386894/150‑1 (MQ=255)
                                                                                                                                                cAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCg       <  2:744679/150‑1 (MQ=255)
                                                                                                                                                cAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCg       <  1:423696/150‑1 (MQ=255)
                                                                                                                                                 aaCTGGTTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCAGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGt      >  1:254717/1‑150 (MQ=255)
                                                                                                                                                 aaCTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAATAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGAGATTGCCCGTGGAATGGTTATTGTAAATGTGCCTTAACGGGCAGGTGATTTaactt      >  1:448576/1‑146 (MQ=255)
                                                                                                                                                   cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  2:305436/150‑1 (MQ=255)
                                                                                                                                                   cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGGCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  1:1558064/150‑1 (MQ=255)
                                                                                                                                                   cTGGGGTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  2:875684/150‑1 (MQ=255)
                                                                                                                                                    tGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtct   <  2:16655/150‑1 (MQ=255)
                                                                                                                                                     gggTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtctc  >  1:1209933/1‑150 (MQ=255)
                                                                                                                                                     gggTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtctc  >  1:1448336/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTC  >  NC_000913/1107512‑1107810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: