Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,107,662 G→A 5.8% W436* (TGG→TGA) ‡ clsC → stationary phase cardiolipin synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,107,6620GA 5.8% 360.2 / 7.9 120W436* (TGG→TGA) ‡clsCstationary phase cardiolipin synthase 3
Reads supporting (aligned to +/- strand):  ref base G (56/57);  new base A (5/2);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

Only 100 of 122 total aligned reads displayed.

CGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTC  >  NC_000913/1107515‑1107810
                                                                                                                                                   |                                                                                                                                                    
cGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGt                                                                                                                                                    <  1:1249492/150‑1 (MQ=255)
 gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                   >  2:440024/1‑150 (MQ=255)
 gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                   >  2:1558064/1‑150 (MQ=255)
 gATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGtt                                                                                                                                                   >  1:17613/1‑150 (MQ=255)
  aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                  <  2:382733/150‑1 (MQ=255)
  aTCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTg                                                                                                                                                  <  2:199609/150‑1 (MQ=255)
     cgcgTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATc                                                                                                                                               >  2:1159562/1‑150 (MQ=255)
       ccttCAACATTGCTCAATACTGAAAGGGGCTGCGGGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTAGGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGt                                                                                                                                             <  2:1162592/148‑1 (MQ=255)
        gTTCAACATTGCTAATTACTGAAATGGGCGTCGGGATCGAGAGCGAAACGCTGGCTCAGTTAATTGATACTCGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACCCAGTTGATccgt                                                                                                                                            <  1:1044542/150‑4 (MQ=255)
           cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATg                                                                                                                                         <  1:1065445/150‑1 (MQ=255)
           cAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGc                                                                                                                                        >  2:1473919/1‑150 (MQ=255)
                    tCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCaaagaaag                                                                                                                                >  1:34699/1‑150 (MQ=255)
                     cAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGAACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAaagaaaga                                                                                                                               <  1:936172/150‑1 (MQ=255)
                        tACTTAAATGGTATTCCGGATATAGAGGGAAACGCTGTCACAGTTAATTGATAAACGCTATATTCAGAGACAGTTTGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGat                                                                                                                            <  2:157367/150‑1 (MQ=255)
                          cTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGatta                                                                                                                          >  1:1732195/1‑150 (MQ=255)
                            gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  2:363247/1‑150 (MQ=255)
                            gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  1:1720381/1‑150 (MQ=255)
                            gAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGattatt                                                                                                                        >  2:1435337/1‑150 (MQ=255)
                               aTGGGCATCGTTATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGTAAGAGATTATtctc                                                                                                                     <  1:308117/150‑1 (MQ=255)
                                tGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCAAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCa                                                                                                                    <  2:280182/150‑1 (MQ=255)
                                      tCGTGATAGAGAGCGAAACGCTGGCAAAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGTTGCGTCCTGGCAGCTCCGTCTGGACATGTGGGGACTTATCAACTGGGTTGATCGTCATGCAAAGATAGAGATTATTCTCAAAAAAg                                                                                                              <  1:783414/150‑1 (MQ=255)
                                      gcGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAg                                                                                                              <  1:1652647/149‑1 (MQ=255)
                                       cGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGa                                                                                                             <  1:978609/150‑1 (MQ=255)
                                        gTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGaa                                                                                                            >  1:602170/1‑150 (MQ=255)
                                         tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGCTCGTCATGCAAAGAAAGAGATTATTCTAAAAAAAGAAc                                                                                                           >  2:1568848/1‑150 (MQ=255)
                                         tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           >  1:1170547/1‑150 (MQ=255)
                                         tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           >  1:1707180/1‑150 (MQ=255)
                                         tGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAc                                                                                                           <  2:198580/150‑1 (MQ=255)
                                          taTAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTTGCAGCTCCGTCTGGACAGGGGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAcc                                                                                                          <  2:345416/149‑1 (MQ=255)
                                          gATAGAGAGCGAAACGCTGGCACAGGTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAAcc                                                                                                          <  2:1521077/150‑1 (MQ=255)
                                            tAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCg                                                                                                        <  2:17613/150‑1 (MQ=255)
                                             agagagCGAAACGCTGGCACAGTGAATTGATACACGCTTTATTCAGAGCCATTATGAAGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGc                                                                                                       <  2:452533/150‑1 (MQ=255)
                                              gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                      <  2:455521/150‑1 (MQ=255)
                                              gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                      >  1:875684/1‑150 (MQ=255)
                                              gagagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGAAAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcc                                                                                                      >  1:744679/1‑150 (MQ=255)
                                               agagCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGcca                                                                                                     <  2:726739/150‑1 (MQ=255)
                                                        cGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCt                                                                                            <  1:28256/150‑1 (MQ=255)
                                                          cTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTgg                                                                                          <  1:68819/150‑1 (MQ=255)
                                                           tGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGa                                                                                         <  1:335820/150‑1 (MQ=255)
                                                            ggCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGaa                                                                                        >  1:1603080/1‑150 (MQ=255)
                                                             gCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAg                                                                                       <  2:34699/150‑1 (MQ=255)
                                                              cacaGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGc                                                                                      >  2:904694/1‑150 (MQ=255)
                                                                caGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCgg                                                                                    >  1:181110/1‑150 (MQ=255)
                                                                 aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                   <  1:113313/150‑1 (MQ=255)
                                                                 aGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCggg                                                                                   >  1:549933/1‑150 (MQ=255)
                                                                   ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCTTCCAGAAACTGGTGGCGGGtt                                                                                 <  1:1717321/150‑1 (MQ=255)
                                                                   ttAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGtt                                                                                 <  1:1463990/150‑1 (MQ=255)
                                                                     aaTTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCTTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGAGTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTAt                                                                               <  1:260684/150‑1 (MQ=255)
                                                                       ttGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATgg                                                                             <  2:1650389/150‑1 (MQ=255)
                                                                        tGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGt                                                                            >  2:545741/1‑150 (MQ=255)
                                                                         gATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGATTCTGGAAGCGGGTTATGGTc                                                                           >  1:189439/1‑150 (MQ=255)
                                                                          aTAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCa                                                                          >  2:1166998/1‑150 (MQ=255)
                                                                             aaCGCTGTATTCCGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCTAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGAc                                                                       <  2:136270/150‑1 (MQ=255)
                                                                               cGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTg                                                                     >  2:540718/1‑150 (MQ=255)
                                                                                gcgtgaTTCAGAGCCAGGATGATGCGGCCTGGCAGCTCCGTCTGGACCGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTgg                                                                    <  1:163070/145‑1 (MQ=255)
                                                                                gCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTgg                                                                    <  2:1712718/150‑1 (MQ=255)
                                                                                  tttATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCg                                                                  <  2:1637515/150‑1 (MQ=255)
                                                                                     aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                               >  2:1386046/1‑150 (MQ=255)
                                                                                     aTTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCg                                                               >  1:631029/1‑150 (MQ=255)
                                                                                         agagCCATTATGATGCGGCCTGGAAGCTCCTTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATTGTCAGACTGGCGTCGatat                                                           <  1:203401/150‑1 (MQ=255)
                                                                                          gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCTATAtt                                                          >  1:320380/1‑150 (MQ=255)
                                                                                          gagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATAtt                                                          >  1:45249/1‑150 (MQ=255)
                                                                                                  atgacgCGGCCTGGCAGCTCCGTTTGGACAGGGGGGGACGGCTCAACGCGGTTGATCGTCATGCAAAGAAAGAGCTTATACTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATAGCCCGTgg                                                  <  2:102084/150‑1 (MQ=255)
                                                                                                   tgatgCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCCCCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGa                                                 >  1:197567/1‑150 (MQ=255)
                                                                                                       gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGCAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTACTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTTGAATgg                                             >  2:1177607/1‑150 (MQ=255)
                                                                                                       gCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATgg                                             <  2:1603080/150‑1 (MQ=255)
                                                                                                        cGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGt                                            <  1:1532735/150‑1 (MQ=255)
                                                                                                        cGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGt                                            <  1:1435337/150‑1 (MQ=255)
                                                                                                         ggCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGAATAATCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGTAATGGtt                                           >  1:47563/1‑150 (MQ=255)
                                                                                                          gCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTa                                          >  2:1018036/1‑150 (MQ=255)
                                                                                                            cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                        >  1:106340/1‑150 (MQ=255)
                                                                                                            cTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTAtt                                        >  1:293903/1‑150 (MQ=255)
                                                                                                             ggctggCTCCTTCTGGACAGGTGGGGACGGATCTACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGATGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTg                                       <  2:723356/145‑1 (MQ=255)
                                                                                                                  gCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAAt                                  <  2:432529/150‑1 (MQ=255)
                                                                                                                    tCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAAtgt                                >  1:297896/1‑150 (MQ=255)
                                                                                                                         cTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTATGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCtt                           >  1:1218049/1‑150 (MQ=255)
                                                                                                                           ggACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTaa                         >  2:229951/1‑150 (MQ=255)
                                                                                                                             aCAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACg                       <  1:958269/150‑1 (MQ=255)
                                                                                                                                   ggggACGGATCTACTTGGTTGATCGTGATGCAACGAAAGAGATTATTCTCAAAAAAGAACCCGCAACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                 <  2:1080999/150‑1 (MQ=255)
                                                                                                                                   ggggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTgg                 >  2:869412/1‑150 (MQ=255)
                                                                                                                                    catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt                >  2:905482/7‑150 (MQ=255)
                                                                                                                                    catgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGt                >  1:1361179/7‑150 (MQ=255)
                                                                                                                                     ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               <  2:1720381/150‑1 (MQ=255)
                                                                                                                                     ggACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               <  1:1018036/150‑1 (MQ=255)
                                                                                                                                     atgacgATCAACCCAGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTg               >  1:648406/6‑150 (MQ=255)
                                                                                                                                      gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              >  2:386894/1‑150 (MQ=255)
                                                                                                                                      gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              >  2:164753/1‑150 (MQ=255)
                                                                                                                                      gACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGc              <  1:540718/150‑1 (MQ=255)
                                                                                                                                        cGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCtt            <  1:1110541/150‑1 (MQ=255)
                                                                                                                                          gATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATTGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTa          >  2:331266/1‑150 (MQ=255)
                                                                                                                                           aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt         <  1:386894/150‑1 (MQ=255)
                                                                                                                                           aTCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTAt         >  1:600087/1‑150 (MQ=255)
                                                                                                                                             cAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCg       <  2:744679/150‑1 (MQ=255)
                                                                                                                                              aaCTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAATAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGAGATTGCCCGTGGAATGGTTATTGTAAATGTGCCTTAACGGGCAGGTGATTTaactt      >  1:448576/1‑146 (MQ=255)
                                                                                                                                                cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  2:305436/150‑1 (MQ=255)
                                                                                                                                                cTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGGCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  1:1558064/150‑1 (MQ=255)
                                                                                                                                                cTGGGGTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtc    <  2:875684/150‑1 (MQ=255)
                                                                                                                                                 tGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtct   <  2:16655/150‑1 (MQ=255)
                                                                                                                                                  gggTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtctc  >  1:1209933/1‑150 (MQ=255)
                                                                                                                                                  gggTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTtctc  >  1:1448336/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTC  >  NC_000913/1107515‑1107810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: