Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,113,544 G→A 7.6% intergenic (+138/‑35) opgH → / → yceK OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,113,5440GA 7.6% 361.7 / 10.3 117intergenic (+138/‑35)opgH/yceKOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (50/58);  new base A (5/4);  total (55/62)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTG  >  NC_000913/1113395‑1113687
                                                                                                                                                     |                                                                                                                                               
gCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCg                                                                                                                                                 <  2:1528112/150‑1 (MQ=255)
gCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCg                                                                                                                                                 >  2:701703/1‑150 (MQ=255)
 cTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTAGCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGt                                                                                                                                                <  1:726843/150‑1 (MQ=255)
  ttCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGtt                                                                                                                                               <  1:1517455/150‑1 (MQ=255)
     gCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATACGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATg                                                                                                                                            >  1:343360/1‑150 (MQ=255)
      cAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                           >  2:393082/1‑150 (MQ=255)
      cAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                           >  2:286719/1‑150 (MQ=255)
       aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCTTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGcc                                                                                                                                          <  1:787226/150‑1 (MQ=255)
       aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGcc                                                                                                                                          <  2:1266676/150‑1 (MQ=255)
       aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACAGGCGTTATGcc                                                                                                                                          >  1:1602132/1‑150 (MQ=255)
         tAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                                                                        <  2:569038/150‑1 (MQ=255)
         aaaaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                                                                        <  1:614248/149‑1 (MQ=255)
          aaaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACACCGAGCCAATAAAAATACCGGCGTTATGCCGGt                                                                                                                                       >  2:907578/1‑150 (MQ=255)
          aaaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGt                                                                                                                                       >  2:590282/1‑150 (MQ=255)
           aaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTa                                                                                                                                      >  1:570433/1‑150 (MQ=255)
           aaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTa                                                                                                                                      <  2:724791/150‑1 (MQ=255)
               cGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAtttt                                                                                                                                  <  1:152755/150‑1 (MQ=255)
               cGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttt                                                                                                                                  <  2:448884/150‑1 (MQ=255)
                gTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAttttt                                                                                                                                 <  1:1013008/150‑1 (MQ=255)
                  aGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttttt                                                                                                                               <  2:1637348/150‑1 (MQ=255)
                  aGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAttttttt                                                                                                                               >  1:1529077/1‑150 (MQ=255)
                   gTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                              <  2:16078/150‑1 (MQ=255)
                    ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTAc                                                                                                                             <  2:1339598/150‑1 (MQ=255)
                    ttGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGTAtttttattg                                                                                                                             <  2:567974/150‑5 (MQ=255)
                      gCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGa                                                                                                                           >  1:1730766/1‑150 (MQ=255)
                      gCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGa                                                                                                                           >  1:1616140/1‑150 (MQ=255)
                            tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAgg                                                                                                                     <  1:997369/150‑1 (MQ=255)
                                     cTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATg                                                                                                            <  1:286719/150‑1 (MQ=255)
                                       tATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGATGTATCAAATGCg                                                                                                          <  1:752357/150‑1 (MQ=255)
                                         tCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                                                                        <  1:645935/150‑1 (MQ=255)
                                          cAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCAGGTATTTTTTTACGAAAGAGGTATCAAATGCGttt                                                                                                       >  1:1476508/1‑150 (MQ=255)
                                            ggCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAACCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                     >  1:1417689/1‑150 (MQ=255)
                                            ggCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                     >  2:190714/1‑150 (MQ=255)
                                                    tCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtggtg                                                                                             <  2:1029799/150‑1 (MQ=255)
                                                          cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc                                                                                       >  1:840936/1‑150 (MQ=255)
                                                          cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatc                                                                                       >  2:1672282/1‑150 (MQ=255)
                                                           tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatca                                                                                      >  2:179068/1‑150 (MQ=255)
                                                            gCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGcatcat                                                                                     >  1:829159/1‑150 (MQ=255)
                                                            gCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTATAATTGTGGTGAGcatcat                                                                                     >  1:76789/1‑150 (MQ=255)
                                                                   aTTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAcc                                                                              <  2:541391/150‑1 (MQ=255)
                                                                       aTTTTGCAAGACTTTGGGGGTCGGATAAGGCGTTCAAGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                          <  2:1355887/150‑1 (MQ=255)
                                                                         tttgataGACTTTGTGGGTCGGATAAGGCGTTCACGACGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTg                                                                        <  1:1081887/144‑1 (MQ=255)
                                                                         tttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTg                                                                        <  2:62338/150‑1 (MQ=255)
                                                                         tttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTg                                                                        <  2:1661715/150‑1 (MQ=255)
                                                                         tttGCAAGACTTTGTGGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTg                                                                        <  2:389736/150‑1 (MQ=255)
                                                                            gCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                              >  1:1396118/1‑93 (MQ=255)
                                                                            gCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTa                                                                                                                              <  2:1396118/93‑1 (MQ=255)
                                                                            gCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTTATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                     >  2:1058689/1‑150 (MQ=255)
                                                                             cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                                                                    <  1:683237/150‑1 (MQ=255)
                                                                             cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                                                                    >  1:368528/1‑150 (MQ=255)
                                                                               aGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTggt                                                                  <  2:704312/150‑2 (MQ=255)
                                                                                   tttGTGGGTCGGATAAGGCGTTCATGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCtgtg                                                              <  1:849845/150‑1 (MQ=255)
                                                                                       tgGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcag                                                          >  1:318689/1‑150 (MQ=255)
                                                                                        gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCTTCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                         >  1:1655795/1‑150 (MQ=255)
                                                                                        gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                         >  2:379053/1‑150 (MQ=255)
                                                                                        gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagc                                                         >  1:180834/1‑150 (MQ=255)
                                                                                        gggTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTACTGAGTGGCTGTGgcagc                                                         <  1:43947/150‑1 (MQ=255)
                                                                                              gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt                                                   >  2:383378/1‑150 (MQ=255)
                                                                                              gATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCattatt                                                   >  2:1363774/1‑150 (MQ=255)
                                                                                               aTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCAttatta                                                  >  1:58000/1‑150 (MQ=255)
                                                                                               aTAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTAGTTTTTTACGATAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGTCAGCAttatta                                                  >  1:852559/1‑150 (MQ=255)
                                                                                                tAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAg                                                 >  1:1405686/1‑150 (MQ=255)
                                                                                                   ggCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTTTTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCg                                              >  2:598809/1‑150 (MQ=255)
                                                                                                    gCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGCGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGc                                             >  2:1217671/1‑150 (MQ=255)
                                                                                                      gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATTAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGATGTATCAAATGAGGTTAATTGTGGTGAGCATCTTGGTGACCTTTCTGAGTGGCTGTGGCTGCATTTTTAGTCGGAc                                           >  1:1118623/1‑150 (MQ=255)
                                                                                                       ttCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACt                                          <  2:1711141/150‑1 (MQ=255)
                                                                                                           cgccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAc                                      >  1:1116810/1‑150 (MQ=255)
                                                                                                            gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAcc                                     <  1:564495/150‑1 (MQ=255)
                                                                                                            gccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAcc                                     <  1:1708198/150‑1 (MQ=255)
                                                                                                             ccgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCg                                    <  1:433127/150‑1 (MQ=255)
                                                                                                              cgcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCgg                                   <  1:57037/150‑1 (MQ=255)
                                                                                                               gcATCCGGCAAATACATCGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGTTGAGCATCATGGTGAGCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCgcg                                  <  2:774869/150‑3 (MQ=255)
                                                                                                               gcATCCGGCAAAAACAACGAGCCAAAAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGGGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggg                                  <  2:129206/150‑1 (MQ=255)
                                                                                                                   ccGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcag                              >  1:1493619/1‑150 (MQ=255)
                                                                                                                   ccGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcag                              <  2:180834/150‑1 (MQ=255)
                                                                                                                      gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcagggg                           <  2:239764/150‑1 (MQ=255)
                                                                                                                       cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                          >  1:416887/1‑150 (MQ=255)
                                                                                                                        aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggca                         >  1:1305873/1‑150 (MQ=255)
                                                                                                                        aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggca                         <  2:1315967/150‑1 (MQ=255)
                                                                                                                        aaaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTAACAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGCCAGCATTATTAGTCGAACTATACCggggcaggggca                         >  2:1037965/1‑150 (MQ=255)
                                                                                                                         aaaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCAGCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCAt                        <  1:1223994/150‑1 (MQ=255)
                                                                                                                          aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                       <  2:541474/150‑1 (MQ=255)
                                                                                                                          aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                       <  2:1529077/150‑1 (MQ=255)
                                                                                                                          aaaCAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATg                       <  1:701703/150‑1 (MQ=255)
                                                                                                                             caacGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTTTTAGTCGCACTATACCGGGGCAGGGGCATGGCa                    >  1:779123/1‑150 (MQ=255)
                                                                                                                               acGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGGGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGACTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAAc                  >  2:947477/1‑150 (MQ=255)
                                                                                                                                cGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAAcc                 <  2:840936/150‑1 (MQ=255)
                                                                                                                                    ccAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAata             <  2:1730766/150‑1 (MQ=255)
                                                                                                                                     gtaaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat            >  1:1174306/5‑150 (MQ=255)
                                                                                                                                     cAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTAACAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat            >  2:1043492/1‑150 (MQ=255)
                                                                                                                                       aaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtattt          >  1:706200/3‑149 (MQ=255)
                                                                                                                                       aaaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatta          >  1:449779/3‑150 (MQ=255)
                                                                                                                                       aTAAATATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatta          <  1:1657033/150‑1 (MQ=255)
                                                                                                                                       aTAAAAATACCGGCGTTATGCCGGTATTTTTGTACGAATGAGGTATCAAATGAGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGATGTGGCAGCATTATTAGTCGCGCTATACAGGCGCAGGGGCATGGCCACCAttatta          >  1:180025/1‑150 (MQ=255)
                                                                                                                                        aaaaaaTACCGGCATAACGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtattat         >  2:119386/2‑150 (MQ=255)
                                                                                                                                            aaTACCGGCGTTATGCCGGTAATTTTTTACGAAAGAGGTATCAAATGCGTGTAATGCTGGGGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGCGCAGGGGCATGGCAACCAATATTTTCCCg     <  1:674561/150‑1 (MQ=255)
                                                                                                                                              tACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCACATGCGTTTAATTCTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTCGCAGCATTCTTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGgt   <  1:69085/150‑1 (MQ=255)
                                                                                                                                              tACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGgt   <  2:1602132/150‑1 (MQ=255)
                                                                                                                                               aCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGgtg  <  1:1037037/150‑1 (MQ=255)
                                                                                                                                               aCCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGGTTAATTGTGGTGAGCATCATGGTGAGCTTGCTGAGGGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACGAATATTATCCCGgtg  <  1:897940/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GCTTCGCAATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGTATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTG  >  NC_000913/1113395‑1113687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: