Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,129,394 G→C 6.1% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,3940GC 6.1% 305.7 / 9.6 99intergenic (+20/+20)murJ/flgNputative lipid II flippase/export chaperone for FlgK and FlgL
Reads supporting (aligned to +/- strand):  ref base G (51/42);  new base C (3/3);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 121 total aligned reads displayed.

GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGT  >  NC_000913/1129245‑1129543
                                                                                                                                                      |                                                                                                                                                     
gTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcag                                                                                                                                                       >  1:740604/1‑150 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg                                                                                                                                                     >  1:340933/1‑150 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGACGGACGGTGTAAGAATGCATTCAGGCCtgcagtg                                                                                                                                                     >  2:1570871/1‑150 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAAGGCGGTCGTACTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTAAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcaggg                                                                                                                                                     >  1:1506043/1‑148 (MQ=255)
   aTTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc                                                                                                                                                    >  1:1419121/1‑150 (MQ=255)
   aTTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGGGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgc                                                                                                                                                    <  2:1673775/150‑1 (MQ=255)
     tGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                                                                  >  1:560985/1‑150 (MQ=255)
     tGGGTACCATGCCCTGGCGTTTACTGCGTGTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTACGCGGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGCGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                                                                  <  2:1140865/150‑1 (MQ=255)
        gTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGcc                                                                                                                                               <  1:921135/150‑1 (MQ=255)
          aCCATGCCCTGGCGGTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCgg                                                                                                                                             <  2:1109839/150‑1 (MQ=255)
          aCCATGCCCGGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg                                                                                                                                             <  1:624638/150‑1 (MQ=255)
            cATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGTGGATTGCCTCGTACTTAGATTCACAGGCGGTACTGGGATTCAAAGTTAAAGAATTTGCCCGCCGGACGGAGTAACAATGCATTCCTGCCTGCAGAGCAGGCCGgag                                                                                                                                           >  2:1686484/1‑150 (MQ=255)
              tGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCACTGCAGGCCGgaatg                                                                                                                                         <  2:161731/150‑4 (MQ=255)
               gCCCTGGCGTTTACTGCGTTTAATGGCGTTCGTGCTGGCGGGGATTTCAGCGTACTTCGCTGCACTGGCGGTACTTTTCTTCAAAGTTAAAGAATTTGCGCGGCGTACGGTGTAACATTGCATTCCGGCATTCAGTGCAGGCCGGAGATa                                                                                                                                        <  2:793264/150‑1 (MQ=255)
                  cTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCTGCCTGCAGTGCAGGCCGGAGATAATc                                                                                                                                     >  2:1389516/1‑150 (MQ=255)
                  cTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                                                                     >  2:1291481/1‑150 (MQ=255)
                    ggCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCtt                                                                                                                                   >  1:718831/1‑150 (MQ=255)
                     gCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                                                                  <  1:1600374/150‑1 (MQ=255)
                     gCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                                                                  <  2:1580152/150‑1 (MQ=255)
                      cGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGTGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                                                                                 <  1:841428/150‑1 (MQ=255)
                          tACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                                                                             >  1:1125981/1‑150 (MQ=255)
                              gCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGaaa                                                                                                                         >  1:1062116/1‑150 (MQ=255)
                                   tAATGGCGGTCGTTCTGGCGGTGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                                                                    <  2:548372/150‑1 (MQ=255)
                                   tAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCCCTGGCGGTACTGGGCTTCAAAGTTAAAGACTTTGCCCGACGGACGGTGTAACAATGCATTCCGGCCTGCAGTGAAGGCCGGAGATAATCTTCAGATAGAAATCttt                                                                                                                    >  1:97612/1‑150 (MQ=255)
                                    aaTGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgaga                                                                                                                                          <  1:1630249/127‑1 (MQ=255)
                                    aaTGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgaga                                                                                                                                          >  2:1630249/1‑127 (MQ=255)
                                       ggCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAc                                                                                                                >  1:797607/1‑150 (MQ=255)
                                                gCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAt                                                                                                       >  2:562800/1‑150 (MQ=255)
                                                gCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAt                                                                                                       <  1:1077509/150‑1 (MQ=255)
                                                   ggCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg                                                                                                    >  1:323287/1‑150 (MQ=255)
                                                    gCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGTATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgt                                                                                                   <  1:887502/150‑1 (MQ=255)
                                                        ggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGgttgtt                                                                                               >  2:1658383/1‑150 (MQ=255)
                                                         gATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGttgttg                                                                                              <  2:340933/150‑1 (MQ=255)
                                                          aTTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAAGAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGttgttgt                                                                                             >  1:177291/1‑149 (MQ=255)
                                                          aTTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGa                                                                                             >  1:574110/1‑150 (MQ=255)
                                                            tGCCGCGTACTTCGCTGCACTGGCTTTACTGGGATTCAAAGTTAAATAATTTGCCCGCCGGACGGTGTAACATTGCTTTCCGTCCTGCAGTGCAGGCCGGAGTTAATCTTCAGATCGATATCTTTTTACCGCCGCTATGTGTTGTTGAgg                                                                                           <  1:807537/150‑1 (MQ=255)
                                                             gCCGCGTTCTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                                                                          >  2:1363970/1‑150 (MQ=255)
                                                             gCCGCGTACTTCGCTGCACTGGCGGAACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                                                                          <  2:15174/150‑1 (MQ=255)
                                                                  gTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGGGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAc                                                                                     <  1:1224973/150‑1 (MQ=255)
                                                                   tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc                                                                                    <  2:1269341/150‑1 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                  >  1:1260948/1‑150 (MQ=255)
                                                                      ttCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAgg                                                                                           <  1:1643646/140‑1 (MQ=255)
                                                                         gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGc                                                                              <  2:578302/150‑1 (MQ=255)
                                                                          cTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                                                                             >  2:578863/1‑150 (MQ=255)
                                                                            gcacgcgCGGTACTGCGCCTCCTCGTTAATGAATTTGCCCGCTGGACGCTGTAACATTGCATTCCGGCCTGCAGCGCAGGCCGGAGTTAATCTTCATATCGAAATCTTTTTACCGCCGCGATGGGTGGTTGAGGTCTGACCGTTCGcccc                                                                           <  2:1075265/144‑1 (MQ=255)
                                                                            gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCGTCAGCTCGAAATCTTTTTACCGCCGCGATGGGGTGTTGCGGTCTGACCGTTCGcccc                                                                           >  2:959637/1‑150 (MQ=255)
                                                                            gCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCGGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcccc                                                                           <  2:1542668/150‑1 (MQ=255)
                                                                                    ggTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATATTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCg                                                                   >  2:1204158/1‑150 (MQ=255)
                                                                                        cTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCgg                                                               >  1:442782/1‑150 (MQ=255)
                                                                                        cTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCgg                                                               >  1:365845/1‑150 (MQ=255)
                                                                                             cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt                                                          <  1:381373/150‑1 (MQ=255)
                                                                                             cTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCt                                                          >  1:217389/1‑150 (MQ=255)
                                                                                              ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg                                                         <  1:809838/150‑1 (MQ=255)
                                                                                              ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg                                                         <  2:964426/150‑1 (MQ=255)
                                                                                              ttCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGACAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTg                                                         >  2:579656/1‑150 (MQ=255)
                                                                                                   aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACGCCCGCATTGGGTTGTTATGTGATCACCTTACGCCCAATATAGCTACGTTTCGTTATGCg                                                    >  2:1500035/1‑150 (MQ=255)
                                                                                                   aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTTGTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCg                                                    <  2:206119/150‑1 (MQ=255)
                                                                                                    gTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGAAGCgg                                                   >  1:317284/1‑150 (MQ=255)
                                                                                                    gTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCGTTTTACCGCCGCGAGGGGTTGTTGAGGTCTGACCGTTCGCCCCATATTGCGTCGGTTCCTGATGCgg                                                   >  2:894946/1‑150 (MQ=255)
                                                                                                      tAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGGTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGtt                                                 >  2:1299623/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                                >  1:988302/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                                >  1:1256958/1‑150 (MQ=255)
                                                                                                        aaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTc                                               >  2:1473943/1‑150 (MQ=255)
                                                                                                        aaGAATTTGCCCGCCGGAAGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCATCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttta                                               >  1:1249199/1‑149 (MQ=255)
                                                                                                           aaTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAAc                                            <  1:53476/150‑1 (MQ=255)
                                                                                                             tttCCCCGCCGTAGGGTGTAAAATGCCTTCCGGCCGTCCAGTGCTGGCTGTTTTTAACTTTAATTCCTAATTTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACAtt                                         <  1:1500035/150‑1 (MQ=255)
                                                                                                                gCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTc                                       <  2:1715004/150‑1 (MQ=255)
                                                                                                                gCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTc                                       >  2:325781/1‑150 (MQ=255)
                                                                                                                 cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTcc                                      >  1:1013448/1‑150 (MQ=255)
                                                                                                                       ggACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTc                                               >  1:946799/1‑135 (MQ=255)
                                                                                                                       ggACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTc                                               <  2:946799/135‑1 (MQ=255)
                                                                                                                         aCGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGATATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGCCCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTg                              >  2:1621600/1‑150 (MQ=255)
                                                                                                                             tgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGa                          >  1:913300/1‑150 (MQ=255)
                                                                                                                             tgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCAATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGa                          <  2:1419121/150‑1 (MQ=255)
                                                                                                                              gtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTACAGCGCCTGTTGAt                         >  2:1179018/1‑150 (MQ=255)
                                                                                                                              gtAACAAGGCATTCCGGCCTGCAGTGCAGGCCTGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGAt                         <  1:1516923/150‑1 (MQ=255)
                                                                                                                               aaaCAATGCATTCCGGCCTGCAGTTCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCTCCGCGATTGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACTTTTCCAGCGACTGTTGAtt                        <  1:707084/149‑1 (MQ=255)
                                                                                                                                 aCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGAGCGAAATCTTTTTACCGCCGCGATGGGTTGGTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTgc                      <  2:97612/150‑1 (MQ=255)
                                                                                                                                  cAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGATATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTgcg                     >  2:53016/1‑150 (MQ=255)
                                                                                                                                   aaTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATTTATGGTCGGGTACTGATGCGGTTTAAACATTTCAAGCGGCTGTTGATTgcgc                    >  2:865192/1‑150 (MQ=255)
                                                                                                                                    aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCt                   <  2:574110/150‑1 (MQ=255)
                                                                                                                                      gCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCa                 >  2:907100/1‑150 (MQ=255)
                                                                                                                                        tctCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCaaa               >  2:761378/3‑149 (MQ=255)
                                                                                                                                          tCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCt             >  2:1684178/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg            >  1:1491135/1‑150 (MQ=255)
                                                                                                                                             ggCCTGCAGTGCAGGCCGGAGATAATCTTCAGCTCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGCTGCGGTTTCAACATTTCCAGCGCCTGTTGAGTGCGCTCAATCTGTc          >  1:1620103/1‑150 (MQ=255)
                                                                                                                                             ggCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTc          <  1:372599/150‑1 (MQ=255)
                                                                                                                                               ccTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCt        >  1:1013314/1‑150 (MQ=255)
                                                                                                                                                   tcagtgcagGCCGGAGATAAGATTCAGATCGAAATCTTTTTACCGCCGCGATGGCTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     <  2:282976/149‑1 (MQ=255)
                                                                                                                                                   gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     >  2:1125990/1‑150 (MQ=255)
                                                                                                                                                   gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     >  2:1289568/1‑150 (MQ=255)
                                                                                                                                                   gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAGGGGTTGTTGAGGTCTGACCGTTCGCACCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     >  2:1514671/1‑150 (MQ=255)
                                                                                                                                                   gcagtgcagGCCGGAGATAAACTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     <  2:463012/150‑1 (MQ=255)
                                                                                                                                                     agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAg   <  2:365845/150‑1 (MQ=255)
                                                                                                                                                     agtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAg   <  1:1291481/150‑1 (MQ=255)
                                                                                                                                                     agtgcagGCCGGAGATAATCTTCAGAGCGAAATCTTATTCCCGCCGCGATGGGGTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTGCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAg   <  1:856719/150‑1 (MQ=255)
                                                                                                                                                      gtgctgGGCGGAGATTATCTTCAGATCGAATTCTTTTTACCGCCGCGATGGGTTGTTGTGGTCTGTTCGTTCGCCCCATTTAGCGTCTGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGt  <  1:1674156/150‑1 (MQ=255)
                                                                                                                                                      gtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGTGTTCAATCTGTCCTTCCAGt  >  2:461595/1‑150 (MQ=255)
                                                                                                                                                      gtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGt  >  1:586866/1‑150 (MQ=255)
                                                                                                                                                      gtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGt  >  2:1408429/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGT  >  NC_000913/1129245‑1129543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: