Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 95,070 G→A 6.0% V141I (GTA→ATA) ‡ murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091395,0700GA 6.0% 363.6 / 5.5 114V141I (GTA→ATA) ‡murFUDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base G (68/39);  new base A (3/4);  total (71/43)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

CTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGA  >  NC_000913/94921‑95218
                                                                                                                                                     |                                                                                                                                                    
cTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGgtg                                                                                                                                                      >  2:626727/1‑150 (MQ=255)
cTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGgtg                                                                                                                                                      >  2:54150/1‑150 (MQ=255)
    aTGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAcc                                                                                                                                                  <  1:1444006/150‑1 (MQ=255)
      gggTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGa                                                                                                                                                >  1:69951/1‑150 (MQ=255)
        gTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATg                                                                                                                                              <  1:962836/150‑1 (MQ=255)
        gTTCGCCAGCAAGTTCCGGCGCGAGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTACGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATg                                                                                                                                              >  1:214406/1‑150 (MQ=255)
         ttCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGa                                                                                                                                             >  1:1703518/1‑150 (MQ=255)
             ccAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCt                                                                                                                                         >  2:710855/1‑150 (MQ=255)
             ccAGCAAGTTCCGGCGCGCGTGGTTGATCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAAAGACATCGGTGTACCGATGACGCt                                                                                                                                         >  2:509545/1‑150 (MQ=255)
                gCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGtt                                                                                                                                      >  2:580481/1‑150 (MQ=255)
                 cAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAGCGTCATCGGTACACCGATGTCGttgttg                                                                                                                                     <  2:518796/150‑1 (MQ=255)
                  aaGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTgc                                                                                                                                    <  1:1439127/150‑1 (MQ=255)
                   aGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTgcg                                                                                                                                   >  2:500949/1‑150 (MQ=255)
                    gTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGTCATCGGTACACCGATGTCGttgttgaga                                                                                                                                  <  2:1453755/150‑4 (MQ=255)
                     ttCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAGCGTCATCGGTACACCGATGTCGttgttgagat                                                                                                                                 <  2:447111/150‑5 (MQ=38)
                        cGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTaa                                                                                                                              >  1:1194439/1‑150 (MQ=255)
                            gcgcgTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGcc                                                                                                                          <  1:844935/150‑1 (MQ=255)
                             cgcgTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCACCACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCg                                                                                                                         >  2:1500141/1‑150 (MQ=255)
                                gTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGaa                                                                                                                      >  2:542797/1‑150 (MQ=255)
                                   gTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTACACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATAc                                                                                                                   <  2:338032/150‑1 (MQ=255)
                                      gCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAt                                                                                                                <  1:674547/150‑1 (MQ=255)
                                       ctctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAtt                                                                                                               >  1:1618042/1‑150 (MQ=255)
                                         ctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTAc                                                                                                             >  2:758672/1‑150 (MQ=255)
                                         ctGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTAc                                                                                                             >  1:806838/1‑150 (MQ=255)
                                         cagACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGCCATCGGTGTACCGATGACGCTGTTGCGCTGAACGCCGGAATACGATTAc                                                                                                             >  2:1497778/3‑150 (MQ=255)
                                          tGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACg                                                                                                            >  1:747494/1‑150 (MQ=255)
                                           gACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGATGCTGTTGCGCTTAACGCCGGAATACGATTACGc                                                                                                           >  1:331994/1‑150 (MQ=255)
                                           gACGGGGTCCTCCGGCAAAACCGCCGATAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGc                                                                                                           <  2:327675/150‑1 (MQ=255)
                                                 gTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATATGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAt                                                                                                     >  1:1303789/1‑150 (MQ=255)
                                                   cctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTg                                                                                                   <  2:69951/150‑1 (MQ=255)
                                                    ctccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACACCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGa                                                                                                  >  2:640340/1‑150 (MQ=255)
                                                     tccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGaa                                                                                                 <  1:1310251/150‑1 (MQ=255)
                                                      ccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAAc                                                                                                <  2:1261766/150‑1 (MQ=255)
                                                      ccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAAc                                                                                                >  2:618938/1‑150 (MQ=255)
                                                      ccGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAAc                                                                                                <  2:1446729/150‑1 (MQ=255)
                                                        ggCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACtt                                                                                              <  1:1437362/150‑1 (MQ=255)
                                                           aaaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgc                                                                                           <  1:1079210/150‑1 (MQ=255)
                                                           aaaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgc                                                                                           <  1:811839/150‑1 (MQ=255)
                                                            aaaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgcg                                                                                          <  1:1147386/150‑1 (MQ=255)
                                                            aaaCCTCAGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgcg                                                                                          >  1:646150/1‑150 (MQ=255)
                                                             aaCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgcgc                                                                                         <  2:468132/150‑1 (MQ=255)
                                                              aCCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGcgcg                                                                                        >  2:935108/1‑150 (MQ=255)
                                                                   cGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCa                                                                                   >  1:616970/1‑150 (MQ=255)
                                                                    gTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCAt                                                                                  >  1:1026971/1‑150 (MQ=255)
                                                                       aaaGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAg                                                                               >  2:1378090/1‑150 (MQ=255)
                                                                       aaaGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAg                                                                               >  2:1216094/1‑150 (MQ=255)
                                                                           agaTGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCg                                                                           >  1:216501/1‑150 (MQ=255)
                                                                            gaTGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGa                                                                          >  1:1016231/1‑150 (MQ=255)
                                                                              tGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGaaa                                                                        >  1:1250966/1‑150 (MQ=255)
                                                                                 cggcggCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAg                                                                     <  2:1703518/150‑1 (MQ=255)
                                                                                  ggcggcGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGc                                                                    <  2:1194439/150‑1 (MQ=255)
                                                                                     ggcgATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTg                                                                 >  2:410244/1‑150 (MQ=255)
                                                                                            ttAAGCCAGTGCGTCAACAAGCTTTATACGGCAGGCAATCTCAACAACTACATCGTTGTACCTATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACtgtg                                                          <  2:1173461/150‑1 (MQ=255)
                                                                                             tAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGa                                                         >  2:460388/1‑150 (MQ=255)
                                                                                              aaGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAg                                                        <  1:906251/150‑1 (MQ=255)
                                                                                              aaGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAg                                                        <  1:161536/150‑1 (MQ=255)
                                                                                               aGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                       >  1:1001741/1‑150 (MQ=255)
                                                                                               aGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGt                                                       <  2:1538144/150‑1 (MQ=255)
                                                                                               aGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACATGGCGCGAACCATCAGGGCGAAATAGCCTGGACTTTGAGt                                                       >  1:302528/1‑150 (MQ=255)
                                                                                                gCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTc                                                      >  2:360308/1‑150 (MQ=255)
                                                                                                 ccAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGCCGCTGTTGCGCGTAACGCCGGAATACGATTACGCTGTTTTTGAACTTGGCGCGAACCCTCAGGGCGAAATCGCCTGGACTGTGAGTCt                                                     >  2:298274/1‑150 (MQ=255)
                                                                                                 ccAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCt                                                     <  1:82674/150‑1 (MQ=255)
                                                                                                       cGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGGACCGATGACGCTGTTGCGCTTAACGCCGGATTACGATTACGCTGTTATTGAACTTGGCTCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCg                                               >  2:1449183/1‑150 (MQ=255)
                                                                                                          cAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGccc                                            >  2:426913/1‑150 (MQ=255)
                                                                                                             cacGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGa                                         >  1:632853/1‑150 (MQ=255)
                                                                                                               cGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAg                                       <  1:542797/150‑1 (MQ=255)
                                                                                                                 cTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGATCCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCt                                     >  1:418028/1‑150 (MQ=255)
                                                                                                                  tttATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTg                                    <  2:418633/150‑1 (MQ=255)
                                                                                                                     ataCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTgcgc                                 <  1:357458/150‑1 (MQ=255)
                                                                                                                     ataCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTgcgc                                 <  1:758672/150‑1 (MQ=255)
                                                                                                                       aCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTg                               <  1:626727/150‑1 (MQ=255)
                                                                                                                       aCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTg                               >  1:216399/1‑150 (MQ=255)
                                                                                                                       aCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTg                               >  2:626396/1‑150 (MQ=255)
                                                                                                                       aCGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAATACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTg                               >  1:916521/1‑150 (MQ=255)
                                                                                                                        cGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTgg                              >  2:1624639/1‑150 (MQ=255)
                                                                                                                         ggcaggcaATCTCAACAACGACTTCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGt                             <  2:729178/150‑1 (MQ=255)
                                                                                                                          gcaggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTc                            <  1:1553350/150‑1 (MQ=255)
                                                                                                                          gcaggcaAGCTCAACAACGACCTCGGTGTACCGATGACGCTGTTGCGCTTACCGACGGAATACGATTACGCAGTTATTGAACTTGGCGCTAACCATCAGGGCGAAATAGCCTGGACTGTGAGTATGACTCGCCCGGAAGCTGCGCTGGTc                            <  2:832844/150‑1 (MQ=255)
                                                                                                                             ggcaATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTcaac                         >  1:1476141/1‑150 (MQ=255)
                                                                                                                               caATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCGTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAACTAGCCTGAACTGTGCGTCTGACTCGCCCGGAAGCTGCGCTGGTcaacaa                       >  1:350438/1‑150 (MQ=255)
                                                                                                                                aagcgcaacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCaacaac                      >  1:1246384/6‑150 (MQ=38)
                                                                                                                                aaTCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCaacaac                      >  2:1243401/1‑150 (MQ=255)
                                                                                                                                 aTCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAAAACGATTACGCAGTTATTGAAATTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAAcc                     >  2:481314/1‑150 (MQ=255)
                                                                                                                                  tctcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCt                    <  1:500949/150‑1 (MQ=255)
                                                                                                                                   cgcaacagCGTCATCGGTACACCGATGTCGTTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTg                   >  1:979978/3‑150 (MQ=39)
                                                                                                                                    tcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACACCATgg                  >  1:450628/1‑150 (MQ=255)
                                                                                                                                    tcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTgg                  <  1:426913/150‑1 (MQ=255)
                                                                                                                                    tcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGAGAACCATCAGGGCGAAATAGCATGGACTGTGAGTCTGACTCGCACGGAAGCTGCGCTGGTCAACAACCTgg                  >  1:444184/1‑150 (MQ=255)
                                                                                                                                    tcAACAACGACATCGGTGTACCGATGACGCTGTTGCGCATAACGCCGGAATACGATTACGCAGTTATTGAACTTTGCGCGAACCATCAGCGCGAAATACCCTGGACTGTGAGTATGACTCGCCAGGAAGCTGCGCTGGTCAACAACCTgg                  >  1:1049257/1‑150 (MQ=255)
                                                                                                                                    tcAACAACGACATCGGGGTACCGATGACGCTGTTGCGCTTAACGCCTGAATACGATTACGCAGTTATTGAACTTGGCGAGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAGCAACCTgg                  >  2:263044/1‑150 (MQ=255)
                                                                                                                                     caacaaCGACATCGGTGTACCGATGACGCTGTTGCGCTTACCGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                 >  2:920157/1‑150 (MQ=255)
                                                                                                                                     caacaaCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                 >  2:926171/1‑150 (MQ=255)
                                                                                                                                     caacaaCGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGc                 >  2:430019/1‑150 (MQ=255)
                                                                                                                                      aacagcGTCATCGGTACACCGATGTCGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCa                >  1:1041689/1‑150 (MQ=255)
                                                                                                                                        caacGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgc              >  1:554231/1‑150 (MQ=255)
                                                                                                                                        caacGACATCGGTGTACCGATGACGCTGTTGCGCATAACGCCGGAATACGATTACGCAGTTATTGAAGTTGGCGCGAACCATCATGGCGAAATATCCTGGACTGTTAGTATGACTCTCGCGGAAGCATCGCTTGTCAACAAACTGGCAgc              >  2:865587/1‑150 (MQ=255)
                                                                                                                                            gACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAgcggcg          >  2:663953/1‑150 (MQ=255)
                                                                                                                                             tcATCGGTGAACCGATGACGCTGTTGCGCTAAAGGCCTGAATACTATTACGCAGATATTGAACTAGGCGCGAACCATCAGGGCGAAATCGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGgcgc         <  1:1497778/149‑1 (MQ=255)
                                                                                                                                                tCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATATGATTACGCAGTTATTGAACTCGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATc      <  1:580481/150‑1 (MQ=255)
                                                                                                                                                    tgtACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGa  <  1:360308/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACCATCAGGGCGAAATAGCCTGGACTGTGAGTCTGACTCGCCCGGAAGCTGCGCTGGTCAACAACCTGGCAGCGGCGCATCTGGA  >  NC_000913/94921‑95218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: