Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,146,739 A→T 5.5% noncoding (151/169 nt) psrD → novel Novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,146,7390AT 5.5% 325.4 / 6.6 109noncoding (151/169 nt)psrDnovel Novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (54/49);  new base T (2/4);  total (56/53)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

Only 100 of 114 total aligned reads displayed.

AGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTG  >  NC_000913/1146590‑1146887
                                                                                                                                                     |                                                                                                                                                    
aGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACca                                                                                                                                                      >  2:774192/1‑150 (MQ=255)
 ggCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACcac                                                                                                                                                     >  2:1265474/1‑150 (MQ=255)
  gCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACcaca                                                                                                                                                    <  1:766636/150‑1 (MQ=255)
   cATATTTTTTTCCATCAGATATAGCGTATTGATCATAGCCATTTTAAACTATGCGATTCGTTTTGCAGGTAGATTATTGTTATCAGCACTGAACGAAAATAAAGCAGTAGCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAg                                                                                                                                                   >  1:88578/1‑150 (MQ=255)
    atatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAgg                                                                                                                                                  >  1:1392394/1‑150 (MQ=255)
    atatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAgg                                                                                                                                                  >  2:24168/1‑150 (MQ=255)
     tatTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGc                                                                                                                                                 >  1:1651099/1‑150 (MQ=255)
         tttttCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACATGATGcc                                                                                                                                             >  1:45286/1‑150 (MQ=255)
            ttCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCttt                                                                                                                                          <  2:198887/150‑1 (MQ=255)
              ccATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCttttt                                                                                                                                        >  2:1587011/1‑150 (MQ=255)
              ccATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAAATAAAATAAAGCAGTAACCCGCAATGTGTGCTAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttg                                                                                                                                        <  2:778205/150‑2 (MQ=255)
              ccATCAGATATAGCGTATTGATGATAGCCATTTTAAACTAAGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTTACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttg                                                                                                                                        <  2:150876/150‑2 (MQ=255)
               cATCAGATATAGCGTATTGATGAAAGCCATTTTAAACTATGGGCTTCGTTTTGCAGGTTGATGTTTGTTATAAGCACTGAACGAAAATTAAGCAGAAACCCGCAATGTGTGCGAATAATTGGCAAAAGGCAACCACAGGCTGCCTTATTc                                                                                                                                       >  2:1173251/1‑150 (MQ=255)
                 tCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCtt                                                                                                                                     <  1:484728/150‑1 (MQ=255)
                  cAGATATATCGTATTGATGATAGACATTTTATACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTTAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAGCCTGTGGTTGCCttttgccaa                                                                                                                                    <  2:846295/150‑6 (MQ=255)
                   aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAATATATAGCTGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                   <  1:1487206/150‑1 (MQ=255)
                   aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                   <  2:882341/150‑1 (MQ=255)
                   aGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTg                                                                                                                                   <  1:593388/150‑1 (MQ=255)
                    gATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGa                                                                                                                                  <  2:1373302/150‑1 (MQ=255)
                     atatAGCGTATTGATGATAGCCATTTTAAACTATGCGCGTCGTTTTGCAGGGTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTATCCCGCAATGTGTGCGAATTATTTGCAAAAGGCAACCACAGTCTGCCTTTTTCTTTTAc                                                                                                                                 >  1:266460/1‑150 (MQ=255)
                       ataGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACATGCTGCCTTTTTCTATGActc                                                                                                                               >  2:305948/1‑150 (MQ=255)
                        taGCGTATTGATGATAGCCATTTTACACTATGCGCTTCGTTTTGCAGGTTGATGCTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCTCAGGCTGCCTTTTTCTTTGActct                                                                                                                              <  2:1196171/150‑1 (MQ=255)
                        taGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGActct                                                                                                                              <  2:650917/150‑1 (MQ=255)
                        taGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAAACACAGGCTGCCTTTTTCTTTGActct                                                                                                                              >  1:1247340/1‑150 (MQ=255)
                         aGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTa                                                                                                                             >  1:719772/1‑150 (MQ=255)
                           cGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATg                                                                                                                           <  1:1623918/150‑1 (MQ=255)
                                      tAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAg                                                                                                                >  2:639711/1‑150 (MQ=255)
                                       aGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGt                                                                                                               >  2:505292/1‑150 (MQ=255)
                                        gCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGtt                                                                                                              >  2:364741/1‑150 (MQ=255)
                                        gCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGtt                                                                                                              >  2:1110984/1‑150 (MQ=255)
                                        gCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGtt                                                                                                              >  1:1327610/1‑150 (MQ=255)
                                         ccATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTa                                                                                                             <  1:1074484/150‑1 (MQ=255)
                                           aTTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAat                                                                                                           <  1:364741/150‑1 (MQ=255)
                                            ttttAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAata                                                                                                          <  1:910806/150‑1 (MQ=255)
                                               tAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgc                                                                                                       >  1:600506/1‑150 (MQ=255)
                                                aaaCTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCTCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcg                                                                                                      <  2:1265981/150‑1 (MQ=255)
                                                aaaCTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACAACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcg                                                                                                      >  2:85453/1‑150 (MQ=255)
                                                  aCTACGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATgcgcg                                                                                                    <  2:1465807/150‑1 (MQ=255)
                                                     atccgcATCGTTTTGCAGGTAGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGGGAATTAATGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGccc                                                                                                 <  2:127013/147‑1 (MQ=255)
                                                     aTGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGccc                                                                                                 <  2:1470704/150‑1 (MQ=255)
                                                        cgcTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTATTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTAt                                                                                              >  1:1318071/1‑150 (MQ=255)
                                                             cGTTTTGCAGGTTGATGTTTGTTATCAGCAGTGAACGAAAATAAAGCAGTAACCAGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCAATGACGTTACAAAGTTAATATGCGCGCCCTATGCaaa                                                                                         <  1:301898/150‑1 (MQ=255)
                                                               ttttGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAg                                                                                       <  1:1368298/150‑1 (MQ=255)
                                                                   gCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTaa                                                                                   >  2:1318725/1‑150 (MQ=255)
                                                                   gCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTaa                                                                                   >  2:772015/1‑150 (MQ=255)
                                                                      ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCTAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                <  1:864093/150‑1 (MQ=255)
                                                                      ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                >  1:1224382/1‑150 (MQ=255)
                                                                      ggTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAt                                                                                <  1:408364/150‑1 (MQ=255)
                                                                       gTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAAtt                                                                               >  1:144709/1‑150 (MQ=255)
                                                                          gATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTAcc                                                                            <  2:1651099/150‑1 (MQ=255)
                                                                             gtttgttATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCt                                                                         >  2:671104/1‑150 (MQ=255)
                                                                                    aTCAGCACTGAACGAAAATAAAGCAGTAACCCGCATTGTTTTCGAATTAATGGCAAAAGGCAACAACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACtctc                                                                  <  2:1395904/150‑1 (MQ=255)
                                                                                    aTCAGCACTGAACGAAAATAAAGCAGTAACACGCAATTGGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGAAGTTACAAACTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACtctc                                                                  <  2:286855/150‑1 (MQ=255)
                                                                                     tCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCg                                                                 <  2:197017/150‑1 (MQ=255)
                                                                                      cAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTAATCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGa                                                                >  1:190276/1‑150 (MQ=255)
                                                                                           cTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCgg                                                           >  1:542942/1‑150 (MQ=255)
                                                                                            tGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGt                                                          <  1:505292/150‑1 (MQ=255)
                                                                                              aaCGAAAATAAAGCAGTAACCCGATATGTTTGATAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTc                                                        <  1:106658/150‑1 (MQ=255)
                                                                                               aCGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAATGTAAAATTACCCCTGACTCTCGATCCGGTTCg                                                       >  1:602681/1‑150 (MQ=255)
                                                                                                cgatattAAAGCAGTAACCCGCATTGTGTGCGAATTATTGGCAAAAGGCAACGACAGGCTGCGTTTTTCTTTGACTCTATGACGTTAGAAAGTTTATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGCCTCTCGGTCCGGTTCGt                                                      >  2:34705/7‑150 (MQ=255)
                                                                                                cGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                      >  2:214131/1‑150 (MQ=255)
                                                                                                cGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGt                                                      <  1:495819/150‑1 (MQ=255)
                                                                                                      tAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCt                                                >  2:1454743/1‑150 (MQ=255)
                                                                                                       aaaGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTc                                               <  1:1644009/150‑1 (MQ=255)
                                                                                                           cAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaaaa                                           <  1:1265474/150‑1 (MQ=255)
                                                                                                           cAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTAGACTCTATGACGTTACAAAGTGAATATGTGAGCACGATGCAAAAGGTAAAATTCCCCCAGACTCTCGATCCGGGGCGTACGCATCaaac                                           >  2:1094638/1‑149 (MQ=255)
                                                                                                            aGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaaaaa                                          >  2:15836/1‑150 (MQ=255)
                                                                                                            aGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCaaaaa                                          >  2:158519/1‑150 (MQ=255)
                                                                                                             gTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAGTTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAAc                                         >  2:781230/1‑150 (MQ=255)
                                                                                                             gTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAAc                                         <  1:879365/150‑1 (MQ=255)
                                                                                                                aCCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACTTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGcc                                      <  2:1273363/150‑1 (MQ=255)
                                                                                                                 cccGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCt                                     >  2:1319334/1‑150 (MQ=255)
                                                                                                                 cccGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCt                                     <  2:1392394/150‑1 (MQ=255)
                                                                                                                 cccGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCt                                     >  1:537047/1‑150 (MQ=255)
                                                                                                                  ccGCAATGTGTGCGAATTATTGGCAAAAGTCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCtt                                    <  1:881749/150‑1 (MQ=255)
                                                                                                                   cGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTg                                   <  1:1587011/150‑1 (MQ=255)
                                                                                                                          tgtgCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGTCTCAAAAACGCCTTGATTACCa                            <  2:600506/150‑1 (MQ=255)
                                                                                                                           gtgCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAg                           <  1:639711/150‑1 (MQ=255)
                                                                                                                             gCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAggg                         <  1:1225977/150‑1 (MQ=255)
                                                                                                                              cGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCATGACTCACGATCCGGTTCGTACGGCTCAAAAAGGCCTTGAGTACCAGGGt                        >  1:1554530/1‑150 (MQ=255)
                                                                                                                                 attattTGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACTAAGTTAATATGAGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATc                     <  1:85453/150‑1 (MQ=255)
                                                                                                                                 attattGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATc                     <  1:1500231/150‑1 (MQ=255)
                                                                                                                                 ataattGGCAAAAGGCAACCACAGGCTGGCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATc                     <  1:305948/147‑1 (MQ=255)
                                                                                                                                  ttattGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCt                    <  1:1454743/150‑1 (MQ=255)
                                                                                                                                    caaagaaaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAAAGCCTTGATTACCAGGGTATCtat                  >  2:439821/7‑150 (MQ=255)
                                                                                                                                    attGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCtat                  >  1:739719/1‑150 (MQ=255)
                                                                                                                                      tGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAc                >  1:404193/1‑150 (MQ=255)
                                                                                                                                         cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc             >  2:328176/1‑150 (MQ=255)
                                                                                                                                         cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATAcccc             >  1:846453/1‑150 (MQ=255)
                                                                                                                                         cAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACAAGGGTATCTATAcccc             >  2:551384/1‑150 (MQ=255)
                                                                                                                                          aaaaGGCAGCCTGTGGTTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCt            >  1:1337432/1‑150 (MQ=255)
                                                                                                                                          aaaaGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCt            >  1:805820/1‑150 (MQ=255)
                                                                                                                                           aaaGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTg           <  2:513125/150‑1 (MQ=255)
                                                                                                                                                cAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATTCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAg      <  1:1110984/150‑1 (MQ=255)
                                                                                                                                                cAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAg      >  1:627316/1‑150 (MQ=255)
                                                                                                                                                  aCCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAACTTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGt    <  1:1554079/150‑1 (MQ=255)
                                                                                                                                                   ccACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGtt   >  1:1308828/1‑150 (MQ=255)
                                                                                                                                                   ccAAAGGCTGCCTTTTTCTTTGAATCTATGACGTTAAAAAGTTAATATGCTCGCCCTATTAAAAAGTTAAAATTACGCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCATTGATTACAAGGGTATCTATACCCCTGATCAGGtt   <  1:1041258/150‑1 (MQ=255)
                                                                                                                                                    cacaGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAATAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTTTCTTTACCCCTGATCAGGTTg  <  1:24168/150‑1 (MQ=255)
                                                                                                                                                    cacaGGCTGCCTTTTTATTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTg  <  2:1318071/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGGCATATTTTTTTCCATCAGATATAGCGTATTGATGATAGCCATTTTAAACTATGCGCTTCGTTTTGCAGGTTGATGTTTGTTATCAGCACTGAACGAAAATAAAGCAGTAACCCGCAATGTGTGCGAATTATTGGCAAAAGGCAACCACAGGCTGCCTTTTTCTTTGACTCTATGACGTTACAAAGTTAATATGCGCGCCCTATGCAAAAGGTAAAATTACCCCTGACTCTCGATCCGGTTCGTACGGCTCAAAAACGCCTTGATTACCAGGGTATCTATACCCCTGATCAGGTTG  >  NC_000913/1146590‑1146887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: