Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,150,471 A→G 5.1% V248V (GTA→GTG fabD → malonyl‑CoA‑[acyl‑carrier‑protein] transacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,150,4710AG 5.1% 353.4 / 5.5 115V248V (GTA→GTGfabDmalonyl‑CoA‑[acyl‑carrier‑protein] transacylase
Reads supporting (aligned to +/- strand):  ref base A (48/61);  new base G (4/2);  total (52/63)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TCTCACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAA  >  NC_000913/1150325‑1150620
                                                                                                                                                  |                                                                                                                                                     
tctcACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGt                                                                                                                                                    >  1:599240/1‑150 (MQ=255)
gctcACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGt                                                                                                                                                    <  2:175957/149‑1 (MQ=255)
   cACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAg                                                                                                                                                 <  2:1158553/150‑1 (MQ=255)
      tgtgCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTg                                                                                                                                              <  2:847302/150‑1 (MQ=255)
      tgtgCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTg                                                                                                                                              <  2:695330/150‑1 (MQ=255)
      tgtgCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTg                                                                                                                                              <  2:495820/150‑1 (MQ=255)
      tgtgCGATGATGAAAAAAGCAGACGACAAACTTGAAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTACCTGTTGTGAAAAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTg                                                                                                                                              <  2:310609/150‑1 (MQ=255)
        tgCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGTACCAGTGCGTCacggatg                                                                                                                                            <  2:810600/150‑7 (MQ=255)
         gcgcTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGtat                                                                                                                                           >  1:451150/1‑150 (MQ=255)
          cgcTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTATCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGtata                                                                                                                                          <  1:320984/150‑1 (MQ=255)
          cgcTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGtata                                                                                                                                          >  1:1652671/1‑150 (MQ=255)
             tgatgaAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAAcc                                                                                                                                       >  1:1346135/1‑150 (MQ=255)
             tgatgaAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAAcc                                                                                                                                       <  1:1012027/150‑1 (MQ=255)
              gatgaAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGCCGCACTGGTACGTCAGTTGTATAAccc                                                                                                                                      >  2:1549749/1‑150 (MQ=255)
               atgaAACCAGCAGCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCgg                                                                                                                                    <  2:1405209/150‑1 (MQ=255)
                tgaAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCgg                                                                                                                                    >  2:1385867/1‑150 (MQ=255)
                   aaCCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCCAAACCAATCGAGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTc                                                                                                                                 >  1:1616642/1‑150 (MQ=255)
                   aaCCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTAGTGAATAACGTTGATGTGAAATGCGCAACCAAAGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTc                                                                                                                                 >  1:1564165/1‑150 (MQ=255)
                     ccAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGGGAATAACGTTGATGTGAAATGCTAAACCAATGGTGATGCGATCCGTGACGCACTGGTAGGTCAGTTGTATATCCCggggctg                                                                                                                               >  2:332830/1‑145 (MQ=255)
                      cagcagCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGGTGATGTGAAATGCGAAACCACTGGTGGTGGCATCCGTGGCGCGCTGGTGCGTCTGGTGTATAGCCCGGGTCAGt                                                                                                                              >  2:1608309/1‑150 (MQ=255)
                        gcagcCGACAATCTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTgg                                                                                                                            <  1:1366694/150‑1 (MQ=255)
                        gcagcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTgg                                                                                                                            <  2:1006449/150‑1 (MQ=255)
                        gcagcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTgg                                                                                                                            <  2:883133/150‑1 (MQ=255)
                        gcagcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTgg                                                                                                                            <  1:1419736/150‑1 (MQ=255)
                          agcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGAc                                                                                                                          <  1:152327/150‑1 (MQ=255)
                          agcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGAc                                                                                                                          >  1:1219919/1‑150 (MQ=255)
                           gcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACg                                                                                                                         >  2:382650/1‑150 (MQ=255)
                           gcCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACg                                                                                                                         >  1:12912/1‑150 (MQ=255)
                            ccGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCACCAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATCTTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGa                                                                                                                        >  1:229940/1‑150 (MQ=255)
                            ccGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGa                                                                                                                        <  1:67081/150‑1 (MQ=255)
                             cGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGaa                                                                                                                       >  1:112741/1‑150 (MQ=255)
                             cGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGaa                                                                                                                       >  2:557979/1‑150 (MQ=255)
                              gACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACAGGTACGTCAGTTGTAAAACCCGGTTCAGTGGACGaat                                                                                                                      >  2:947261/1‑149 (MQ=255)
                               aCAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGt                                                                                                                     <  2:944258/150‑1 (MQ=255)
                                cAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTc                                                                                                                    >  2:83821/1‑150 (MQ=255)
                                  aaCTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGAGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTg                                                                                                                  >  1:1467677/1‑150 (MQ=255)
                                    cTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGtt                                                                                                                <  1:485757/150‑1 (MQ=255)
                                     tGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTg                                                                                                               <  1:1434613/150‑1 (MQ=255)
                                       gCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAAAGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAg                                                                                                             <  2:281586/150‑1 (MQ=255)
                                             gAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATg                                                                                                       >  1:1212443/1‑150 (MQ=255)
                                               aTTAGCGAAAATCACCTTTAACGCACTAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGc                                                                                                     <  2:454066/150‑1 (MQ=255)
                                                 tAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAg                                                                                                   <  2:1705586/150‑1 (MQ=255)
                                                  aGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAgc                                                                                                  <  2:745778/150‑1 (MQ=255)
                                                   gCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAgcg                                                                                                 >  2:1301304/1‑150 (MQ=255)
                                                    cGAAAATCACCTTTAACGCACCAGCAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAgcgc                                                                                                >  2:30787/1‑150 (MQ=255)
                                                    cGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTTGACGAAGTCTGTTGAGTACATGGCAgcgc                                                                                                >  1:1048651/1‑150 (MQ=255)
                                                    cGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAgcgc                                                                                                >  1:17559/1‑150 (MQ=255)
                                                             ccTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAg                                                                                       <  2:457037/150‑1 (MQ=255)
                                                             ccTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAg                                                                                       <  2:451150/150‑1 (MQ=255)
                                                             ccTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAg                                                                                       <  1:802296/150‑1 (MQ=255)
                                                              cTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGa                                                                                      >  1:1617988/1‑150 (MQ=255)
                                                              cTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGa                                                                                      <  1:808102/150‑1 (MQ=255)
                                                                ttAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAAc                                                                                    <  2:365039/150‑1 (MQ=255)
                                                                    cGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGAACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACAtct                                                                                <  2:436797/150‑1 (MQ=255)
                                                                     gCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACAtctc                                                                               <  1:557979/150‑1 (MQ=255)
                                                                        ccAACTTTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCGATCCGTGACGCACTGTTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGATCATCTCTAt                                                                            <  1:52394/150‑1 (MQ=255)
                                                                        ccAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACACGGTTCAGCGGACGAAGTCTCTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTAt                                                                            >  1:344819/1‑150 (MQ=255)
                                                                         cAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATg                                                                           <  2:599240/150‑1 (MQ=255)
                                                                         cAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATg                                                                           <  1:1386856/150‑1 (MQ=255)
                                                                           aCAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGaa                                                                         >  2:1206316/1‑150 (MQ=255)
                                                                              ggtCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTc                                                                      <  1:46910/148‑1 (MQ=255)
                                                                              gTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTc                                                                      <  1:972778/150‑1 (MQ=255)
                                                                                 ccTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGc                                                                   >  2:1033768/1‑150 (MQ=255)
                                                                                     ttgtGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCgg                                                               >  2:1636018/1‑150 (MQ=255)
                                                                                      tgtgAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCggg                                                              >  1:827150/1‑150 (MQ=255)
                                                                                         gAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCaa                                                           >  2:434179/1‑150 (MQ=255)
                                                                                           aTAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGt                                                                                                            <  2:286078/99‑1 (MQ=255)
                                                                                                 ttGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTa                                                   <  1:1221100/150‑1 (MQ=255)
                                                                                                  tGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTAc                                                  <  1:1665093/150‑1 (MQ=255)
                                                                                                   gATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATACCCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACCTCTCTATGAAGTCGGCCCGGGCAAACTGCTTACt                                                 >  1:265949/1‑150 (MQ=255)
                                                                                                    aTGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTg                                                <  2:1581281/150‑1 (MQ=255)
                                                                                                       tgAAATGCTAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGcc                                             <  1:1173905/150‑1 (MQ=255)
                                                                                                       tgAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGcc                                             <  1:83821/150‑1 (MQ=255)
                                                                                                        gAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCt                                            >  1:1452280/1‑150 (MQ=255)
                                                                                                        gAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTAAGGGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCGCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCt                                            >  1:1246962/1‑150 (MQ=255)
                                                                                                          aaTGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGa                                          <  2:1212443/150‑1 (MQ=255)
                                                                                                          aaTGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGa                                          >  1:1728605/1‑150 (MQ=255)
                                                                                                            tGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTGTGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACg                                        <  1:1608309/150‑1 (MQ=255)
                                                                                                              cGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGaa                                      >  1:973266/1‑150 (MQ=255)
                                                                                                                 aaCCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACg                                   <  2:1471069/150‑1 (MQ=255)
                                                                                                                   ccAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCa                                 <  2:12912/150‑1 (MQ=255)
                                                                                                                    cAATGGTGATGCCATCCGTGACGCACTGGTGCGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCAt                                >  1:668302/1‑150 (MQ=255)
                                                                                                                    cAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGACCGGGCAAAGTGCTTACTGGCCTGACGAAACGCAt                                >  1:148752/1‑150 (MQ=255)
                                                                                                                       tGGTGATGCCATCCGTGACGCAATGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGt                             <  2:1048651/150‑1 (MQ=255)
                                                                                                                         gTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGACCATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGACTGACGAAACGCATTGTCg                           >  2:1517439/1‑150 (MQ=255)
                                                                                                                              gCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGTACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACAcc                      <  2:769084/150‑1 (MQ=255)
                                                                                                                                 acaactGACGTACCAGTGCGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTg                   >  1:828943/6‑150 (MQ=255)
                                                                                                                                 aTCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTg                   <  2:17559/150‑1 (MQ=255)
                                                                                                                                 aTCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTg                   >  1:1248507/1‑150 (MQ=255)
                                                                                                                                   ccGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGAAGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGACCATCTCTATCAAGTCGGCCCTGGCAAAGTGCTTACTGTCCTGACGAAACGCATTGTCGACACCCTGAc                 >  1:837806/1‑150 (MQ=255)
                                                                                                                                    actGACGTACCAGTGCGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGAcc                >  1:573898/3‑150 (MQ=255)
                                                                                                                                      tGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGc              >  1:998071/1‑150 (MQ=255)
                                                                                                                                       gccGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGTCAGCGCAAGGCGTATAACATCTCTATGAAGGCTGACCGGGCAAAGTGATTACTGGCCTGACGAAACGCATTGTCGAAACCCTGACCGcc             <  2:1494926/148‑1 (MQ=255)
                                                                                                                                           cACTGGTACGTCAGTTGTATAACACGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACAGGACTGACGAAAAGCATTGTCGACACCCTGACCGCCTCgg         >  2:1707415/1‑150 (MQ=255)
                                                                                                                                            tcTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTCAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCAGTGTCGACAGCCTGACCGCCTCGgc        >  1:280340/2‑150 (MQ=255)
                                                                                                                                             cTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGgcg       <  2:1655573/150‑1 (MQ=255)
                                                                                                                                               ggTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCt     <  1:1278471/150‑1 (MQ=255)
                                                                                                                                               ggTACGTAAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGAAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGAAACCCTTACAGCCTCGgcgat     >  1:306274/1‑148 (MQ=255)
                                                                                                                                                 tACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCGGTTGAGTACATGGCAGCGCAAGGCGGAGAACATCTCTAGGATGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCAGTGTCGCCACCCTGACCGCCTCGGCGCTga   >  2:69941/1‑150 (MQ=255)
                                                                                                                                                  aCGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTCTGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTgaa  <  1:1385867/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TCTCACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAA  >  NC_000913/1150325‑1150620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: