Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,157,633 A→T 11.5% intergenic (+59/‑236) ycfH → / → ptsG putative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,157,6330AT11.5% 290.7 / 20.2 111intergenic (+59/‑236)ycfH/ptsGputative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (43/55);  new base T (6/7);  total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GTTGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGC  >  NC_000913/1157485‑1157778
                                                                                                                                                    |                                                                                                                                                 
gtTGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAAt                                                                                                                                                  <  1:54457/150‑1 (MQ=255)
  tGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTg                                                                                                                                                <  2:420817/150‑1 (MQ=255)
     cGTTGAAGAACTGGCTCAGTTAACCAACGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTTAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGc                                                                                                                                             <  1:905252/150‑1 (MQ=255)
     cGTTGAAGAACTGGCGCAGGAAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCATCCCGCCTTCAATCCATCCGTTGACTGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGc                                                                                                                                             <  2:1111576/150‑1 (MQ=255)
      gTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCg                                                                                                                                            >  2:521586/1‑150 (MQ=255)
       ttGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCgg                                                                                                                                           <  1:594778/150‑1 (MQ=255)
        tGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGt                                                                                                                                          <  1:1076387/150‑1 (MQ=255)
        tGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGt                                                                                                                                          <  1:1177581/150‑1 (MQ=255)
        tGAAGAACTGGCGCAGGTAACCACCGATAACTTAGCAAGTCTGTTTCACATCGACGCATCCCGCATTCAATCCATCCGGTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAAACAGTAAAGTTCACCGACGATAATTGGGCTGt                                                                                                                                          >  1:281869/1‑150 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTg                                                                                                                                         >  1:985948/1‑150 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTg                                                                                                                                         <  2:642585/150‑1 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTg                                                                                                                                         <  2:568888/150‑1 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTg                                                                                                                                         <  2:828678/150‑1 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGACGAAAATTGGGCGGTg                                                                                                                                         >  2:1335430/1‑150 (MQ=255)
         gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACAGCGGAAAATTGGGCGGTg                                                                                                                                         >  1:172806/1‑150 (MQ=255)
           agaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGaa                                                                                                                                       >  1:330978/1‑150 (MQ=255)
            gaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAAt                                                                                                                                      >  1:825057/1‑150 (MQ=255)
            gaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTACCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTGaac                                                                                                                                      <  1:1490045/150‑2 (MQ=255)
             aaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATa                                                                                                                                     <  2:607177/150‑1 (MQ=255)
             aaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTGaact                                                                                                                                     <  1:1541875/150‑3 (MQ=255)
                 ggCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCa                                                                                                                                 >  1:444108/1‑150 (MQ=255)
                    gcAGGTAACCACCGATAACTTCGCCCGGCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGt                                                                                                                              <  2:1556922/150‑1 (MQ=255)
                     cAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGtt                                                                                                                             <  2:705023/150‑1 (MQ=255)
                     cAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGtt                                                                                                                             >  2:732146/1‑150 (MQ=255)
                           accaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAat                                                                                                                       >  2:1350083/1‑150 (MQ=255)
                           accaccGAAAACTTCGCCCGTCTGTTTCACTTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAat                                                                                                                       <  1:731551/150‑1 (MQ=255)
                            ccaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAata                                                                                                                      >  1:916697/1‑150 (MQ=255)
                              accGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATAtt                                                                                                                    <  1:732146/150‑1 (MQ=255)
                               ccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATtg                                                                                                                   <  2:863269/150‑1 (MQ=255)
                                 gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCTGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATtgtg                                                                                                                 <  1:1413315/150‑1 (MQ=255)
                                 gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATtgtg                                                                                                                 <  1:216658/150‑1 (MQ=255)
                                 gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATtgtg                                                                                                                 <  2:1655579/150‑1 (MQ=255)
                                 gATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATtgtg                                                                                                                 <  2:1703871/150‑1 (MQ=255)
                                   tAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGAc                                                                                                               <  2:934893/150‑1 (MQ=255)
                                   tAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGAc                                                                                                               <  1:1090152/150‑1 (MQ=255)
                                   tAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGAc                                                                                                               <  1:1436475/150‑1 (MQ=255)
                                        tCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATg                                                                                                          <  1:1514173/150‑1 (MQ=255)
                                             cGTCTGTTTCACATCGACGCTACCCGCCATCAATCAATCCGTTGAATGATTTTTTTTAAAGCTCTTAATTAATTGCTAAAACGAGTAAAGTTAAACGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTg                                                                                                     <  1:865685/150‑1 (MQ=255)
                                              gTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGt                                                                                                    <  2:852374/150‑1 (MQ=255)
                                                    ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                              >  1:354933/1‑150 (MQ=255)
                                                       acaTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAtgtg                                                                                           <  2:593992/150‑1 (MQ=255)
                                                       acaTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAtgtg                                                                                           >  1:1069407/1‑150 (MQ=255)
                                                        caTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAATGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGc                                                                                          <  1:425340/150‑1 (MQ=255)
                                                        caTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGc                                                                                          <  1:632317/150‑1 (MQ=255)
                                                          tCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCaa                                                                                        >  2:937373/1‑150 (MQ=255)
                                                            gACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACt                                                                                      >  1:541024/1‑150 (MQ=255)
                                                             aCGCTTCCCGCCTTCTATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAACTTAACCGCCGATATTTGGGCGGTGAATAACCACGTTTGAAATATTGAGAAATATGTTTTGTGAAAATGTTCAACtt                                                                                     >  1:278033/1‑150 (MQ=255)
                                                              cGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACtta                                                                                    >  2:1636111/1‑149 (MQ=255)
                                                               gCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTtct                                                                                   <  1:1525335/150‑1 (MQ=255)
                                                                   cccGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCaa                                                                               >  1:1695498/1‑150 (MQ=255)
                                                                   cccGCCTTCAATCCATACGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCTCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCaa                                                                               >  2:658448/1‑150 (MQ=255)
                                                                        cTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATc                                                                          <  2:1218525/150‑1 (MQ=255)
                                                                          tCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTg                                                                        >  2:1697361/1‑150 (MQ=255)
                                                                           cAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGa                                                                       >  2:1244725/1‑150 (MQ=255)
                                                                            aaTCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGaa                                                                      <  2:825057/150‑1 (MQ=255)
                                                                             atccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAg                                                                     <  1:1376558/150‑1 (MQ=255)
                                                                               ccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGtt                                                                   <  1:529868/150‑1 (MQ=255)
                                                                                   ccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTATCCAATGATCTGAAGTTGaaa                                                               >  1:138654/1‑150 (MQ=255)
                                                                                     gTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACg                                                             <  1:767355/150‑1 (MQ=255)
                                                                                        gAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTAGTCAAAATGTGCAACATCTCCAATGATCTGAAGTTGAAACTTGa                                                          >  2:401845/1‑150 (MQ=255)
                                                                                             aGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGcc                                                     <  2:541024/150‑1 (MQ=255)
                                                                                             aGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTCGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGcc                                                     >  1:690005/1‑150 (MQ=255)
                                                                                              gTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCg                                                    <  2:880401/150‑1 (MQ=255)
                                                                                              gTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCg                                                    <  1:493827/150‑1 (MQ=255)
                                                                                               tttttttAAATCTCGTAATTAATGGATAAAACGAGTAAAGTTCACCGCCGAAAATTGGGAGGTTAATAACCTCGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGt                                                   <  2:813273/150‑1 (MQ=255)
                                                                                                ttttttAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTc                                                  <  1:937489/150‑1 (MQ=255)
                                                                                                  ttttAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaa                                                >  1:1065714/1‑150 (MQ=255)
                                                                                                         gCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAAtt                                         <  1:1350083/150‑1 (MQ=255)
                                                                                                          cTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAAAGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTg                                        >  2:1239936/1‑150 (MQ=255)
                                                                                                           tCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTgg                                       >  1:1421140/1‑150 (MQ=255)
                                                                                                           tCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTgg                                       <  2:488656/150‑1 (MQ=255)
                                                                                                            cGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTTGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGc                                      <  1:1513422/150‑1 (MQ=255)
                                                                                                             gTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTCAAATATTGTGACAAATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGTCa                                     >  1:72343/1‑150 (MQ=255)
                                                                                                             gTAATTAATGGCTAAAACGAGTAAAGTTCACAGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCa                                     <  2:1288716/150‑1 (MQ=255)
                                                                                                               aattaatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACt                                   <  2:126377/150‑1 (MQ=255)
                                                                                                                       gCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAattatt                           >  1:1733292/1‑150 (MQ=255)
                                                                                                                        cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                          >  2:1144241/1‑150 (MQ=255)
                                                                                                                        cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                          >  1:1661870/1‑150 (MQ=255)
                                                                                                                        cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                          <  2:1069407/150‑1 (MQ=255)
                                                                                                                          aaaaCGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTa                        >  1:1429297/1‑150 (MQ=255)
                                                                                                                          aaaaCGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTa                        >  1:766467/1‑150 (MQ=255)
                                                                                                                           aaaCGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTAc                       <  1:960934/150‑1 (MQ=255)
                                                                                                                             aCGAGTACAGTTCACCGCCTAAAATAGGGCGGGGAATAACCCCGTTTGAAAAATTGTGTCATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTActc                     <  2:262561/150‑1 (MQ=255)
                                                                                                                              cGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTActct                    >  1:790973/1‑150 (MQ=255)
                                                                                                                                  tAAAGTTCACGGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGGCAAACAAATTGGCACTGAATTATTTTACTCTgtgt                <  2:904299/150‑1 (MQ=255)
                                                                                                                                    aatttCACCTCCGAAATTTTTTCGGTGAATAACCACTTTTGAAATATTGTGACATATGTTTTGTCAAAATGTTCAACTTCTCTAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaa              <  2:3577/147‑1 (MQ=255)
                                                                                                                                    aaGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGTCACTGAATTATTTTACTCTGTGtaa              <  2:343502/150‑1 (MQ=255)
                                                                                                                                     tattCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaat             >  2:173150/3‑150 (MQ=255)
                                                                                                                                       ttCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaataa           >  2:1609929/1‑150 (MQ=255)
                                                                                                                                        tCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAATCGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaa          >  2:734387/1‑150 (MQ=255)
                                                                                                                                         cACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCATTGATCTGAAGTTGAAACGTGATAGCCGTCAAACTAATTGGCACTGTATTATTTTAGTCTGTGTaataaat         >  2:1123394/1‑150 (MQ=255)
                                                                                                                                         cACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaat         >  2:540242/1‑150 (MQ=255)
                                                                                                                                         cACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaat         >  1:1382506/1‑150 (MQ=255)
                                                                                                                                         cACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGAACTGAATTATTTTACTCTGTGTaataaat         >  2:1325822/1‑150 (MQ=255)
                                                                                                                                         cACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaat         >  2:1675949/1‑150 (MQ=255)
                                                                                                                                           ccgccgAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTATGTGTAataaataa       >  1:936742/1‑150 (MQ=255)
                                                                                                                                             gccgAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAg     >  1:645396/1‑150 (MQ=255)
                                                                                                                                              ccgAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAgg    <  2:674382/150‑1 (MQ=255)
                                                                                                                                               cgAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAggg   <  1:1244725/150‑1 (MQ=255)
                                                                                                                                                gAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAATTGTGCAACTTCTCCAATGATCTCAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGgc  <  2:172806/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTTGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGC  >  NC_000913/1157485‑1157778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: