BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,174,501	A→T	6.9%	L222Q (CTG→CAG)	ycfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,501	0	A	T	6.9%	274.9 / 6.4	99	L222Q (CTG→CAG)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (53/39); new base T (2/5); total (55/44)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 103 total aligned reads displayed.

CATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAG  >  NC_000913/1174352‑1174649

caTTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCACGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATca                                                                                                                                                      >  1:735308/1‑150 (MQ=255)

   tCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATcagca                                                                                                                                                   >  2:208967/1‑150 (MQ=255)

      gcagGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACACCCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGAt                                                                                                                                                >  2:910897/1‑150 (MQ=255)

           cTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATggcgg                                                                                                                                           <  1:1009410/150‑1 (MQ=255)

              gtggCGCGCATACCGAATCGTTGCTCGTATTGCTAAAACAGCTTCATGATCACCACAATCGCTACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGCCCGGTAAGCAAAGAAGCCATCAGCAGATGGCGgcgc                                                                                                                                        >  2:1003125/1‑150 (MQ=255)

              gtggAGCGCATACCGAATCGTTGCTCGTATTGATAAAAGAGCTTCATGATCACCACAATCGATACGAGCGATAACAGCAGCGAGATAGTAAACAACCAGGCACAGACCGCCAGAACGGTAAGCAAAGAAGCCATCTGCTGATggcttctt                                                                                                                                        <  2:201958/150‑6 (MQ=255)

              gtggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCTGCTGATggcttctt                                                                                                                                        <  1:1693902/150‑6 (MQ=255)

              gtggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGCAAGCAAAGAAGCCATCTGCTGATggcttctt                                                                                                                                        <  2:515250/150‑6 (MQ=255)

               tggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAATGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCa                                                                                                                                       <  1:1352002/150‑1 (MQ=255)

               tggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCTGCTGATggcttcttt                                                                                                                                       <  2:1733777/150‑7 (MQ=255)

                ggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCTGCTGATggcttctttg                                                                                                                                      <  1:562155/150‑8 (MQ=255)

                  aGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACg                                                                                                                                    <  1:1732805/150‑1 (MQ=255)

                    cgcATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACgga                                                                                                                                  <  1:1027504/150‑2 (MQ=255)

                    cgcATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGAACCGACCGCCAGAACGGTAAGCAAAGAAGCCAACAGCAGATGGCGGCGCAACGGc                                                                                                                                  <  1:302128/150‑1 (MQ=255)

                    cgcATACCGAATCGTTGCGCGTATTGATAAAACAGCTTCATGATCACCACAATCGAGACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGc                                                                                                                                  <  1:959995/150‑1 (MQ=255)

                      cATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGCCGGCGCAACGGCAc                                                                                                                                >  1:389633/1‑150 (MQ=255)

                      cATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGACATCAGCAGATGGCGGCGCAACGGCAc                                                                                                                                >  1:438340/1‑150 (MQ=255)

                        tACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGAGACAACAGCAGCGAGATAGTAAACAACCAGGCCCCGACCGCCAGAGCGGTAAGCCAAGACGCCATCAGCAGATGGCGGCGCAACGGCACct                                                                                                                              >  2:273217/1‑150 (MQ=255)

                          ccGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACctct                                                                                                                            >  2:1535598/1‑150 (MQ=255)

                            gAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCttt                                                                                                                          <  2:607038/150‑1 (MQ=255)

                               tCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAac                                                                                                                       <  1:709830/150‑1 (MQ=255)

                               tCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAac                                                                                                                       <  2:467051/150‑1 (MQ=255)

                                 gTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAacac                                                                                                                     >  1:533054/1‑150 (MQ=255)

                                    gCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAgg                                                                                                                  <  1:1127487/150‑1 (MQ=255)

                                      tCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTc                                                                                                                <  2:1402564/150‑1 (MQ=255)

                                       cGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCTATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCa                                                                                                               >  1:438711/1‑150 (MQ=255)

                                       cGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCATCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCa                                                                                                               >  1:340504/1‑150 (MQ=255)

                                            tGATAAAACAGCTTCATGATCACCACAATAGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCattatt                                                                                                          >  2:946739/1‑150 (MQ=255)

                                             gATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACACCCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTAttg                                                                                                         <  1:675826/150‑1 (MQ=255)

                                               tAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTAttgtt                                                                                                       <  2:448422/150‑1 (MQ=255)

                                                aaaaCAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCCGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACAGTAAGCAAAGAAGCCATCAGCAGATGGCGCCGCAACGGCACCTCTATAACCCAGGTCATTAttgtac                                                                                                      >  1:80340/1‑148 (MQ=255)

                                                 aaaCAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTgc                                                                                                     >  2:653980/1‑150 (MQ=255)

                                                        ttCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAAc                                                                                              >  1:1430189/1‑150 (MQ=255)

                                                        ttCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAAc                                                                                              >  1:452876/1‑150 (MQ=255)

                                                         tCATGATCACCACAATCGATACCAGCGACAACCGCAGCGAGATAGTACACAACCAGGCACCGAACGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAAcc                                                                                             <  1:1448985/150‑1 (MQ=255)

                                                         tCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAAcc                                                                                             <  1:1126317/150‑1 (MQ=255)

                                                           aTGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAt                                                                                           >  1:1269636/1‑150 (MQ=255)

                                                            tGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCTCCACGGCACCTCTTTAACACAGGTCATTATTTTTGCGCCAAACCATg                                                                                          >  2:1064103/1‑150 (MQ=255)

                                                              aTCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATgcg                                                                                        <  2:1269636/150‑1 (MQ=255)

                                                              aTCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATgcg                                                                                        <  1:1020111/150‑1 (MQ=255)

                                                                  ccacAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCACACCATGCGCCaa                                                                                    >  1:466752/1‑150 (MQ=255)

                                                                   cacaATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAg                                                                                   <  1:208967/150‑1 (MQ=255)

                                                                    acaATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCCCCGACCGCCAGAACGGTAAGCAAAGCAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAgg                                                                                  >  2:1512298/1‑150 (MQ=255)

                                                                    acaATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAgg                                                                                  >  2:1706065/1‑150 (MQ=255)

                                                                     caATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGc                                                                                 <  1:461591/150‑1 (MQ=255)

                                                                     caATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGc                                                                                 >  2:748481/1‑150 (MQ=255)

                                                                        acGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGt                                                                              <  1:1579353/149‑1 (MQ=255)

                                                                              ccAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCATCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAAGCGCCAAGGCTGTAATAAg                                                                        >  2:453085/1‑150 (MQ=255)

                                                                              ccAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAg                                                                        >  1:983311/1‑150 (MQ=255)

                                                                                aGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGc                                                                      >  2:605330/1‑150 (MQ=255)

                                                                                   gACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCATCGGCACCTCTTTAACACAGGTCATTATTGTTACGCCAAACCATGCGCCAAGGCTGTAATAAGGCaaa                                                                   >  2:733353/1‑150 (MQ=255)

                                                                                      aacaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTg                                                                >  2:1445700/1‑150 (MQ=255)

                                                                                      aacaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTg                                                                >  1:478054/1‑150 (MQ=255)

                                                                                       acaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCTTTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGc                                                               >  1:176911/1‑150 (MQ=255)

                                                                                       acaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGc                                                               >  2:665783/1‑150 (MQ=255)

                                                                                          gcagcGAGATAGTAAACAACCAGGCACCGACCGCCAGATCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGa                                                            <  1:287507/150‑1 (MQ=255)

                                                                                          gcagcGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGa                                                            <  2:1162799/150‑1 (MQ=255)

                                                                                             gcGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATca                                                         >  1:517284/1‑150 (MQ=255)

                                                                                               gagaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCTATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATcaca                                                       <  2:438711/150‑1 (MQ=255)

                                                                                               gagaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATcaca                                                       <  2:340504/150‑1 (MQ=255)

                                                                                                agaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCacac                                                      >  2:1115316/1‑150 (MQ=255)

                                                                                                 gaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACt                                                     <  1:1119283/150‑1 (MQ=255)

                                                                                                  aTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTg                                                    >  2:939398/1‑150 (MQ=255)

                                                                                                    aGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGtt                                                  >  1:1182286/1‑150 (MQ=255)

                                                                                                        aacaacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCAtt                                              >  1:1681360/1‑150 (MQ=255)

                                                                                                          caacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGGCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTcc                                            <  1:1216109/150‑1 (MQ=255)

                                                                                                           aacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAACCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTccc                                           >  1:1061904/1‑150 (MQ=255)

                                                                                                           aacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTccc                                           >  1:1056560/1‑150 (MQ=255)

                                                                                                              cAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAACCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCccac                                        >  1:387011/1‑150 (MQ=255)

                                                                                                              cAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCccac                                        >  2:620602/1‑150 (MQ=255)

                                                                                                              cAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCccac                                        >  1:1566791/1‑150 (MQ=255)

                                                                                                               aGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCccacc                                       <  2:631097/150‑1 (MQ=255)

                                                                                                                     cgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCg                                 <  1:1344657/150‑1 (MQ=255)

                                                                                                                      gaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGt                                >  2:1045732/1‑150 (MQ=255)

                                                                                                                        ccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTgg                              <  2:533054/150‑1 (MQ=255)

                                                                                                                         cgCCAGCTCGGTATGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCCCGGCGTggg                             >  2:616476/1‑150 (MQ=255)

                                                                                                                         cgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGTGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTggg                             <  2:1599768/150‑1 (MQ=255)

                                                                                                                         cgCCAGAACGGTAAGAAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACTCAGGTCAGTATTGTTGCGCAAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCCGATCACACTGATCATTCCCCCCCACGGCGTggg                             >  2:847639/1‑150 (MQ=255)

                                                                                                                            cAGAACGGTAAGCAATGAAGCCATCAGCAGATGGCGGCGCAACGGCACCGCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAAc                          <  1:1165213/150‑1 (MQ=255)

                                                                                                                             aGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACg                         >  1:600278/1‑150 (MQ=255)

                                                                                                                             aGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGAGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACg                         <  2:1625337/150‑1 (MQ=255)

                                                                                                                                 cGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAAt                     <  1:946739/150‑1 (MQ=255)

                                                                                                                                 cGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAAt                     >  2:1568286/1‑150 (MQ=255)

                                                                                                                                     aaGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAACCCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                 >  1:925484/1‑150 (MQ=255)

                                                                                                                                     aaGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGCTCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                 >  2:450722/1‑150 (MQ=255)

                                                                                                                                     aaGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                 >  1:1175699/1‑150 (MQ=255)

                                                                                                                                     aaGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGAAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAAc                 >  1:1628177/1‑150 (MQ=255)

                                                                                                                                     aaGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACAATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGTAACGAAATTaaa                 >  2:1399817/1‑149 (MQ=255)

                                                                                                                                            gccgCCATCTGCTGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca          >  2:382263/4‑150 (MQ=255)

                                                                                                                                            gccgCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcaca          >  1:1388372/4‑150 (MQ=255)

                                                                                                                                             ccgCCATCTGCTGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCAGTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCacac         >  1:1113973/3‑150 (MQ=255)

                                                                                                                                             aagCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCacac         <  2:735308/150‑1 (MQ=255)

                                                                                                                                                ccATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTca      <  1:748481/150‑1 (MQ=255)

                                                                                                                                                ccATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTca      >  1:791301/1‑150 (MQ=255)

                                                                                                                                                ccATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTca      >  1:1006919/1‑150 (MQ=255)

                                                                                                                                                 cATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCcagcaccag     >  2:248940/1‑143 (MQ=255)

                                                                                                                                                 cATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcag     >  1:589880/1‑150 (MQ=255)

                                                                                                                                                  aTCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagc    <  1:939398/150‑1 (MQ=255)

                                                                                                                                                    cagcagATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCCCCACGGCGTGGGCACGAAATTAACCGCCACACTcagcag  >  1:720126/1‑150 (MQ=255)

                                                                                                                                                    cagcagATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTcagcag  >  1:1651341/1‑150 (MQ=255)

CATTCAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAG  >  NC_000913/1174352‑1174649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`