Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,187,092 G→C 6.7% intergenic (+22/+27) pepT → / ← roxA peptidase T/50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,187,0920GC 6.7% 342.6 / 11.8 105intergenic (+22/+27)pepT/roxApeptidase T/50S ribosomal protein L16 arginine hydroxylase; 2‑oxoglutarate oxygenase
Reads supporting (aligned to +/- strand):  ref base G (48/50);  new base C (4/3);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 109 total aligned reads displayed.

CGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGC  >  NC_000913/1186943‑1187238
                                                                                                                                                     |                                                                                                                                                  
cGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTTCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCAtgtg                                                                                                                                                    >  2:334017/1‑150 (MQ=255)
cGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCAtgtg                                                                                                                                                    >  2:1720797/1‑150 (MQ=255)
cGTGACCGAACCTGTTCACTGTCGGTTAGAACTATCATGTGAAGCATGAGTTTGTGACTCTGGAATGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGAGGCGGCAtgtg                                                                                                                                                    <  2:1533520/150‑1 (MQ=255)
  tGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGAt                                                                                                                                                  <  1:168071/150‑1 (MQ=255)
      cGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATTGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCg                                                                                                                                              <  1:1327572/150‑1 (MQ=255)
      cGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGTATGTGATGCCg                                                                                                                                              <  2:630420/150‑1 (MQ=255)
       gAACCTTTTCACTGGCGGTTAAAACTTTCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCTACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGc                                                                                                                                             <  2:741245/150‑1 (MQ=255)
       gAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGc                                                                                                                                             >  2:40121/1‑150 (MQ=255)
          ccTGTTCACTGGCGGTTACAACTATCATGGTAATCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCATc                                                                                                                                          <  2:1290277/150‑1 (MQ=255)
          ccTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATc                                                                                                                                          >  2:1380270/1‑150 (MQ=255)
          ccTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCATc                                                                                                                                          <  2:1664204/150‑1 (MQ=255)
           cggtgcccTGGCGGTGACCACTCTCCTGGGACGCATGCGTTTGTGCCTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGAGCGTCCGTATTGCCGAGTTAACTGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATCACATGCCGCATcc                                                                                                                                         <  1:151904/143‑1 (MQ=255)
           cTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATcc                                                                                                                                         >  1:659931/1‑150 (MQ=255)
            tGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCg                                                                                                                                        >  1:585863/1‑150 (MQ=255)
             gTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCgg                                                                                                                                       >  1:538678/1‑150 (MQ=255)
              ttCACTGTCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGACGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGc                                                                                                                                      <  2:1054229/150‑1 (MQ=255)
              ttCACTGGCGGTTACAACTATGATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGGCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGc                                                                                                                                      <  2:356833/150‑1 (MQ=255)
              ttCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGc                                                                                                                                      <  1:1288346/150‑1 (MQ=255)
              ttCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGc                                                                                                                                      <  1:1207659/150‑1 (MQ=255)
                     gCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATc                                                                                                                               <  1:545259/150‑1 (MQ=255)
                     gCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATc                                                                                                                               >  1:1176421/1‑150 (MQ=255)
                     gCGGTAACAACTATCATGGTATGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATc                                                                                                                               <  2:283906/150‑1 (MQ=255)
                       ggTTACAACTATCATGGTAAGCATGAGATTGTGACTCTGGAAGGTATGGAAAAAGCGGGGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGTATGCGGCATGTGATGCCGCATCCGGCTTAAATCCa                                                                                                                             <  2:887894/150‑1 (MQ=255)
                        gTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAACGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCaa                                                                                                                            <  1:1230071/150‑1 (MQ=255)
                          tACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAAc                                                                                                                          >  1:911675/1‑150 (MQ=255)
                          tACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAAc                                                                                                                          >  2:1271768/1‑150 (MQ=255)
                            cAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACtt                                                                                                                        >  2:460527/1‑150 (MQ=255)
                               cTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTTCAGGTGATCGTCCGTATTGCCGAGATAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTAcc                                                                                                                     <  1:335787/150‑1 (MQ=255)
                                    aTGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCg                                                                                                                <  1:897129/150‑1 (MQ=255)
                                    aTGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCg                                                                                                                <  1:1113351/150‑1 (MQ=255)
                                     tGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCga                                                                                                               >  1:394986/1‑150 (MQ=255)
                                     tGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCga                                                                                                               >  1:1680825/1‑150 (MQ=255)
                                           gCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAAcc                                                                                                         <  1:1045457/150‑1 (MQ=255)
                                           gCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAAcc                                                                                                         <  1:1383686/150‑1 (MQ=255)
                                              tGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAAt                                                                                                      >  1:40684/1‑150 (MQ=255)
                                               gAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATa                                                                                                     >  1:399777/1‑150 (MQ=255)
                                                 gTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATAcc                                                                                                   >  1:96588/1‑150 (MQ=255)
                                                     gtgACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCt                                                                                               >  1:1715127/1‑150 (MQ=255)
                                                      tgACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAAGCCAAACTTACCCTTCGAAGAACCAAGACCCGCTa                                                                                              >  2:318855/1‑150 (MQ=255)
                                                        aCTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGTAGAACCAATACCCGCTAtt                                                                                            <  2:1540018/150‑1 (MQ=255)
                                                         ctctGGAAGCTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTg                                                                                           <  2:1453893/150‑1 (MQ=255)
                                                          tctGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGa                                                                                          <  2:1731148/150‑1 (MQ=255)
                                                            tGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGATAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGAcc                                                                                        >  1:82894/1‑150 (MQ=255)
                                                             ggAAGGTTTGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCa                                                                                       <  1:633767/150‑1 (MQ=255)
                                                             ggAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCa                                                                                       >  1:612012/1‑150 (MQ=255)
                                                                 ggTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAgcgc                                                                                   >  1:288406/1‑150 (MQ=255)
                                                                 ggTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATATGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGAACAgcgc                                                                                   >  2:361347/1‑150 (MQ=255)
                                                                   tATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGcgccg                                                                                 >  2:1109754/1‑150 (MQ=255)
                                                                    aTGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGcgccgc                                                                                >  2:1695116/1‑150 (MQ=255)
                                                                     tGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGAATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCcgcg                                                                               >  2:727542/1‑150 (MQ=255)
                                                                      ggAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGa                                                                              >  1:387501/1‑150 (MQ=255)
                                                                       gAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCTAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAg                                                                             >  2:1298500/1‑150 (MQ=255)
                                                                           aaGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATc                                                                         >  1:27329/1‑150 (MQ=255)
                                                                            aGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTAACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcg                                                                        <  1:1083917/150‑1 (MQ=255)
                                                                              cGGTTCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATcgcg                                                                      <  2:1294171/150‑1 (MQ=255)
                                                                                gTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTAGCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAg                                                                    <  1:334017/150‑1 (MQ=255)
                                                                                gTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAg                                                                    >  1:104205/1‑150 (MQ=255)
                                                                                 tGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAgg                                                                   <  2:659931/150‑1 (MQ=255)
                                                                                  gCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGa                                                                  <  1:503046/150‑1 (MQ=255)
                                                                                   cAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGaa                                                                 <  2:82617/150‑1 (MQ=255)
                                                                                     ggTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAAtc                                                               >  1:649874/1‑149 (MQ=255)
                                                                                        gATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACg                                                            >  1:1446568/1‑150 (MQ=255)
                                                                                        gATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACg                                                            <  1:1380270/150‑1 (MQ=255)
                                                                                         aTCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGCCCAGCGCCGCGAGCATCGCGAGGAATGACgg                                                           >  1:593718/1‑150 (MQ=255)
                                                                                         aTCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACgg                                                           <  2:1709748/150‑1 (MQ=255)
                                                                                              ccGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCtt                                                      >  1:1064139/1‑150 (MQ=255)
                                                                                                gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGCCTGATCTTcc                                                    >  2:1234747/1‑150 (MQ=255)
                                                                                                  aTTGCCGAGTTAACGGCGCAACGGAAGTAAGCGATAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAg                                                  <  2:82894/150‑1 (MQ=255)
                                                                                                   ttGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAgc                                                 <  2:1413032/150‑1 (MQ=255)
                                                                                                    tGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAgcg                                                <  2:1205261/150‑1 (MQ=255)
                                                                                                    tGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAgcg                                                <  1:40121/150‑1 (MQ=255)
                                                                                                      ccGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCa                                              >  2:484249/1‑150 (MQ=255)
                                                                                                       cGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGAATCCGGCTTAAATCCAACATTACCATTCGAAGAACAAATACCCGCTATTGCCCAGCGCCGCGAGCAACGCGAGGAATGACGGATATACCAGCGCaa                                             >  1:757466/1‑149 (MQ=255)
                                                                                                             aaCGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGCCCAGCGCCGCGAGCATCGCGAGTAATGACGGATCTTCAAGCGCATCGCCaa                                       >  2:725525/1‑150 (MQ=255)
                                                                                                             aaCGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGATTGACGGAGCTTCCAGCGCATCGCCaa                                       >  2:116775/1‑150 (MQ=255)
                                                                                                             aaCGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCaa                                       >  2:1347526/1‑150 (MQ=255)
                                                                                                              aCGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCaaa                                      <  1:1109754/150‑1 (MQ=255)
                                                                                                               cGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCGTCCGGCTTAAATCCAAACTTACCATTCGAAAAACCACTACCCGCTATTGACCAGCGCCGCGAGATACCCGAGTAATGACGTATCTTCCAGCGCATAGCCaaaa                                     >  2:93531/1‑150 (MQ=255)
                                                                                                                ggCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAAt                                    <  2:109004/150‑1 (MQ=255)
                                                                                                                 gcgcAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAAtt                                   >  1:1592121/1‑150 (MQ=255)
                                                                                                                   gcAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATtct                                 <  2:399777/150‑1 (MQ=255)
                                                                                                                   gcAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATtct                                 <  2:245427/150‑1 (MQ=255)
                                                                                                                    cAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATtctc                                >  2:1251636/1‑150 (MQ=255)
                                                                                                                       cGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGc                             <  1:460527/150‑1 (MQ=255)
                                                                                                                        ggAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCa                            <  2:911675/150‑1 (MQ=255)
                                                                                                                           aGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTc                         <  1:993170/150‑1 (MQ=255)
                                                                                                                             tAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAg                       >  2:664119/1‑150 (MQ=255)
                                                                                                                             tAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAg                       <  2:14849/150‑1 (MQ=255)
                                                                                                                               aGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAgcg                     <  1:878838/150‑1 (MQ=255)
                                                                                                                               aGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAgcg                     <  1:1113605/150‑1 (MQ=255)
                                                                                                                                   aaaaGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAAt                 >  2:1225266/1‑150 (MQ=255)
                                                                                                                                    aaaGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATg                >  1:1424949/1‑150 (MQ=255)
                                                                                                                                     gccggATGCGGCATCACATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGt               >  1:880552/4‑150 (MQ=255)
                                                                                                                                     gccggATGCGGCATCACATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGt               >  2:440509/4‑150 (MQ=255)
                                                                                                                                     gccggATGCGGCATCACATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGt               >  1:1089857/4‑150 (MQ=255)
                                                                                                                                     aaGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGt               <  1:727542/150‑1 (MQ=255)
                                                                                                                                        ggATGCGGCATCACATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGc            >  2:1371577/1‑150 (MQ=255)
                                                                                                                                         gATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCt           >  1:1029990/1‑150 (MQ=255)
                                                                                                                                           tGCGGCATGGGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg         <  2:1635470/150‑1 (MQ=255)
                                                                                                                                                cATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGt    >  1:376380/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGTGCCCGAACCTGTTCACTGGCGGTTACAACTATCATGGTAAGCATGAGTTTGTGACTCTGGAAGGTATGGAAAAAGCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGC  >  NC_000913/1186943‑1187238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: