Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,212,476 G→C 5.5% intergenic (‑473/+227) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,4760GC 5.5% 400.0 / 11.8 128intergenic (‑473/+227)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base G (51/70);  new base C (3/4);  total (54/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

Only 100 of 133 total aligned reads displayed.

AATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAG  >  NC_000913/1212330‑1212625
                                                                                                                                                  |                                                                                                                                                     
aataatGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCa                                                                                                                                                    <  2:342412/150‑1 (MQ=255)
  taatGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTTCGTTAAGTATTGAAACGTACGTGTGGCACg                                                                                                                                                  >  2:48247/1‑150 (MQ=255)
    atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGc                                                                                                                                                >  2:1679134/1‑150 (MQ=255)
    atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGc                                                                                                                                                <  1:1590233/150‑1 (MQ=255)
    atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGc                                                                                                                                                <  1:62656/150‑1 (MQ=255)
     tGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGcc                                                                                                                                               >  1:334537/1‑150 (MQ=255)
       aaTATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgt                                                                                                                                             <  1:1129662/150‑1 (MQ=255)
         aataAGCATGACATTAGAATAAGGTTTCAATTTTTGATTTATATAGTAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACTTGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgttt                                                                                                                                           <  1:739882/149‑2 (MQ=255)
          ataAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtttc                                                                                                                                          <  1:1239905/150‑3 (MQ=255)
          ataAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtttc                                                                                                                                          <  1:1298165/150‑3 (MQ=255)
           taAGCATGACATGAGAATAAGGATTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCgttgtt                                                                                                                                         <  2:409907/150‑1 (MQ=255)
             aGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                       <  2:148749/150‑1 (MQ=255)
             aGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                       <  2:1671742/150‑1 (MQ=255)
              gCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGTAATGATTTAACATGTTCCTGGCTAAAATACATATACCCGGATGATTACTAAACCAAAATACATGTTCGTTAAGTATTTAAACGTACGTGTGGCACGGCCGTTGttttt                                                                                                                                      <  1:527986/150‑1 (MQ=255)
              gCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGttttt                                                                                                                                      <  2:1366222/150‑1 (MQ=255)
                atgccatgaCAATAAGGTTTCAATTTTTGAGTTATATAGGAAAGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTtat                                                                                                                                    <  1:335272/150‑1 (MQ=255)
                atgacatgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTtat                                                                                                                                    <  1:1407472/150‑1 (MQ=255)
                      tgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAatat                                                                                                                              <  1:481254/150‑1 (MQ=255)
                      tgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATAAAACCGGATTATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACTGACGTGTGGCACGGCCGTTGTTTTTTTAAatat                                                                                                                              <  1:1021756/150‑1 (MQ=255)
                       gagaATAATGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATCCATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATg                                                                                                                             <  2:1732716/150‑1 (MQ=255)
                         gaATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGTACGTGTGGCACGGCCGTTGTTTTTATAAATATGtt                                                                                                                           >  1:1668566/1‑150 (MQ=255)
                               ggTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACAGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGt                                                                                                                     >  2:1639973/1‑150 (MQ=255)
                                gTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGtt                                                                                                                    <  2:441508/150‑1 (MQ=255)
                                 tttCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                   <  1:1445143/150‑1 (MQ=255)
                                  ttCAATTTTTTATTTATATATGAATTTTTTAACCTGTTCCTGGCTAAAATACATTTAACCGGATGATGACTAAACCATAATACATGTGCGTTATGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTTACCGTtat                                                                                                                  <  1:517560/150‑1 (MQ=255)
                                    cAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATaa                                                                                                                >  2:368635/1‑150 (MQ=255)
                                    cAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATaa                                                                                                                <  2:303573/150‑1 (MQ=255)
                                       tttttGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAAt                                                                                                             <  2:1090682/150‑1 (MQ=255)
                                        ttttGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATa                                                                                                            >  1:1648943/1‑150 (MQ=255)
                                        ttttGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATa                                                                                                            >  1:650885/1‑150 (MQ=255)
                                           tGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCTGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACg                                                                                                         <  2:232452/150‑1 (MQ=255)
                                           tGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGTATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACg                                                                                                         <  1:987372/150‑1 (MQ=255)
                                              gTTATATAGTAATGATTTAAACTGTTCCTGGCTAAAATACATATAACCGTATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAAATGACGTGTGTCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTAt                                                                                                      <  1:656812/150‑1 (MQ=255)
                                                 atataGGAATGATTTAACCTGTTCCTGGCTAAAATAAATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATTTTATAACggttagt                                                                                                   <  1:1639973/150‑7 (MQ=255)
                                                    taGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAg                                                                                                >  2:1609081/1‑150 (MQ=255)
                                                     aGGAATGATTTAACCTGTTCCTGGCTTAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAATAGt                                                                                               >  2:1362761/1‑150 (MQ=255)
                                                     aGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGt                                                                                               >  2:1322632/1‑150 (MQ=255)
                                                      ggAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTc                                                                                              >  2:552036/1‑150 (MQ=255)
                                                      ggAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTc                                                                                              <  2:804645/150‑1 (MQ=255)
                                                       gAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCa                                                                                             >  1:100799/1‑150 (MQ=255)
                                                        aaTGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCaa                                                                                            >  2:768438/1‑150 (MQ=255)
                                                           gATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTg                                                                                         >  1:645023/1‑150 (MQ=255)
                                                                 aCCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATcaca                                                                                   <  1:1571306/150‑1 (MQ=255)
                                                                      ttCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCa                                                                              <  2:845123/150‑1 (MQ=255)
                                                                      ttCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCa                                                                              <  2:1235791/150‑1 (MQ=255)
                                                                       tCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaa                                                                             >  2:1187367/1‑150 (MQ=255)
                                                                          tGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAata                                                                          <  1:1436257/150‑1 (MQ=255)
                                                                             cTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATGTGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCa                                                                       <  2:581612/150‑1 (MQ=255)
                                                                                aaaTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGt                                                                    <  2:426177/150‑1 (MQ=255)
                                                                                 aaTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGtt                                                                   <  1:1672319/150‑1 (MQ=255)
                                                                                  aTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTATTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTAAAGTTATCACATTTCAAATATCAAGttt                                                                  >  1:1068166/1‑149 (MQ=255)
                                                                                         taACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTAt                                                           >  1:1640106/1‑150 (MQ=255)
                                                                                            ccGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTa                                                            >  2:502649/1‑146 (MQ=255)
                                                                                              ggATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTc                                                      <  2:8745/150‑1 (MQ=255)
                                                                                                  gatgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGt                                                  <  1:618473/150‑1 (MQ=255)
                                                                                                  gatgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGt                                                  <  1:908151/150‑1 (MQ=255)
                                                                                                   atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTg                                                 <  2:319807/150‑1 (MQ=255)
                                                                                                   atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATAAAGTTGATAGTATTAGTATGGTg                                                 >  1:448218/1‑150 (MQ=255)
                                                                                                     gaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGat                                               <  2:393949/150‑1 (MQ=255)
                                                                                                     gaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGat                                               >  2:399476/1‑150 (MQ=255)
                                                                                                         aaaCCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGattatt                                           >  2:1574383/1‑150 (MQ=255)
                                                                                                         aaaCCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACAATTCAAATATCAAGTTGATATGATTAGTATGGTGattatt                                           >  2:186096/1‑150 (MQ=255)
                                                                                                            ccAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGAttatttat                                        >  2:455291/1‑150 (MQ=255)
                                                                                                              aaaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATgg                                      >  2:667366/1‑150 (MQ=255)
                                                                                                               aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                     <  1:1053186/150‑1 (MQ=255)
                                                                                                               aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                     <  1:552036/150‑1 (MQ=255)
                                                                                                               aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                     <  1:1104452/150‑1 (MQ=255)
                                                                                                                 aTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTg                                   <  2:98464/150‑1 (MQ=255)
                                                                                                                  tACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGa                                  <  2:431083/150‑1 (MQ=255)
                                                                                                                   aCATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGAc                                 <  1:368635/150‑1 (MQ=255)
                                                                                                                       gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGACTGGTGATTATATATGTGAGACAATa                             >  2:1643713/1‑150 (MQ=255)
                                                                                                                        tgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaa                            >  1:300775/1‑150 (MQ=255)
                                                                                                                        tgCGTTAAGTATTGAAACGGACGTGTGGCAAGGCCGTTGTTATTATATATATGTTAACCGTTATAAAATAAAGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTTATAGTATTAGTCTGGTGATTATATATGGGTTACAATaa                            >  1:662966/1‑150 (MQ=255)
                                                                                                                         gCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATTGGTGACAATaaa                           >  2:1307103/1‑150 (MQ=255)
                                                                                                                         gCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaa                           >  1:366207/1‑150 (MQ=255)
                                                                                                                            ttAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAg                        <  2:951189/150‑1 (MQ=255)
                                                                                                                              aaGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGAc                      <  1:1391701/150‑1 (MQ=255)
                                                                                                                              aaGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGAc                      <  2:650885/150‑1 (MQ=255)
                                                                                                                                taaaaacaaCGGCCGTGCCACACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                    >  2:552636/8‑150 (MQ=255)
                                                                                                                                gTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                    <  2:645023/150‑1 (MQ=255)
                                                                                                                                gTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                    <  1:1606241/150‑1 (MQ=255)
                                                                                                                                 aaaaacaaCGGCCGTGCCACACGTCCGTTGTTTTTATAAATATGTTACCCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGt                   >  1:954919/7‑150 (MQ=255)
                                                                                                                                 aaaaacaaCGGCCGTGCCACACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGt                   >  2:990594/7‑150 (MQ=255)
                                                                                                                                  aTTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                  <  1:1148202/150‑1 (MQ=255)
                                                                                                                                  aTTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                  >  1:415679/1‑150 (MQ=255)
                                                                                                                                  aTTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                  >  2:1079016/1‑150 (MQ=255)
                                                                                                                                   ttGAAACGTACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAAGCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTAt                 <  2:855136/150‑1 (MQ=255)
                                                                                                                                    tGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTTACCGTTATAAAATAACGTATAAAAAGTAAATTGATCACATTTCAAATATAAAGTTTATATTATTTTTATTGTGATTATTTATGGGTGACAATAAAAACACattatt                >  2:1550921/1‑150 (MQ=255)
                                                                                                                                    tGAAACGGACGTGTGGCACGGCCGTTGGTTTTAAAAATATGTTTACCGGTTTAAAAGTACGTTTCAAAAGTCAAGGGTAGACATTTGAAATTTGAAGTTTAAAGTTTTATTGTTGTGCTTTTTTTAGGGTGTCAATAAAAAGACAGTAtt                >  2:1564584/1‑150 (MQ=255)
                                                                                                                                     gAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTa               >  2:269055/1‑150 (MQ=255)
                                                                                                                                     gAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTa               >  2:1204515/1‑150 (MQ=255)
                                                                                                                                      aaaCGGACGAGTGGCACGGCCGTTGATTTTATAAATATGTAAACCGTTATAAAATAACGAATCAAAAGTCAAGTGATCACATATCAAATATAAAGATGAATCTATTAGTCTCTTGATTATATATGGGTGACAATAAAAAGAAAGTATTaa              >  1:952/1‑150 (MQ=255)
                                                                                                                                       aaCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAat             <  1:768438/150‑1 (MQ=255)
                                                                                                                                        aCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAatc            >  2:110173/1‑150 (MQ=255)
                                                                                                                                           gACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAatcatc         >  1:29303/1‑150 (MQ=255)
                                                                                                                                               tgtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGCTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATa     >  2:244544/1‑150 (MQ=255)
                                                                                                                                                 tgGCACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATaga   <  2:727524/150‑1 (MQ=255)
                                                                                                                                                  ggCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATagat  >  2:1710200/1‑149 (MQ=255)
                                                                                                                                                  ggCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATagag  >  1:469664/1‑150 (MQ=255)
                                                                                                                                                  ggCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATagag  >  2:1203540/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAG  >  NC_000913/1212330‑1212625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: