Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,212,482 G→T 5.6% intergenic (‑479/+221) iraM ← / ← ycgX RpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,212,4820GT 5.6% 380.9 / 7.3 133intergenic (‑479/+221)iraM/ycgXRpoS stabilzer during Mg starvation, anti‑RssB factor/DUF1398 family protein
Reads supporting (aligned to +/- strand):  ref base G (55/69);  new base T (3/6);  total (58/75)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 133 total aligned reads displayed.

ATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAG  >  NC_000913/1212334‑1212629
                                                                                                                                                    |                                                                                                                                                   
atGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGc                                                                                                                                                    <  1:1590233/150‑1 (MQ=255)
 tGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGcc                                                                                                                                                   >  1:334537/1‑150 (MQ=255)
   aaTATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgt                                                                                                                                                 <  1:1129662/150‑1 (MQ=255)
     tataAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCgttg                                                                                                                                               <  1:366014/150‑1 (MQ=255)
     aataAGCATGACATTAGAATAAGGTTTCAATTTTTGATTTATATAGTAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACTTGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgttt                                                                                                                                               <  1:739882/149‑2 (MQ=255)
      ataAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtttc                                                                                                                                              <  1:1239905/150‑3 (MQ=255)
      ataAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGCCGTGCCACACGTCCgtttc                                                                                                                                              <  1:1298165/150‑3 (MQ=255)
         aGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                           <  2:424731/150‑1 (MQ=255)
         aGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                           <  2:1671742/150‑1 (MQ=255)
         aGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGtttt                                                                                                                                           <  2:148749/150‑1 (MQ=255)
           catgacatgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTta                                                                                                                                         >  1:426177/1‑150 (MQ=255)
            atgacatgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTtat                                                                                                                                        <  1:1407472/150‑1 (MQ=255)
              gacatgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATaa                                                                                                                                      <  2:1351908/150‑1 (MQ=255)
               acatgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATaaa                                                                                                                                     >  2:1606241/1‑150 (MQ=255)
                  tgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAatat                                                                                                                                  >  2:1104452/1‑150 (MQ=255)
                  tgaGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATAAAACCGGATTATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACTGACGTGTGGCACGGCCGTTGTTTTTTTAAatat                                                                                                                                  <  1:1021756/150‑1 (MQ=255)
                   gagaATAATGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATCCATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATg                                                                                                                                 <  2:1732716/150‑1 (MQ=255)
                     gaATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGTACGTGTGGCACGGCCGTTGTTTTTATAAATATGtt                                                                                                                               >  1:1668566/1‑150 (MQ=255)
                             tttCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTta                                                                                                                       <  1:1445143/150‑1 (MQ=255)
                              ttCAATTTTTTATTTATATATGAATTTTTTAACCTGTTCCTGGCTAAAATACATTTAACCGGATGATGACTAAACCATAATACATGTGCGTTATGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTTACCGTtat                                                                                                                      <  1:517560/150‑1 (MQ=255)
                                cAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATaa                                                                                                                    <  2:303573/150‑1 (MQ=255)
                                    ttttGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATa                                                                                                                >  1:650885/1‑150 (MQ=255)
                                    ttttGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATa                                                                                                                >  1:1648943/1‑150 (MQ=255)
                                       tGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCTGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACg                                                                                                             <  2:232452/150‑1 (MQ=255)
                                       tGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGTATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACg                                                                                                             <  1:987372/150‑1 (MQ=255)
                                           ttATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATc                                                                                                         <  1:704611/150‑1 (MQ=255)
                                             atataGGAATGATTTAACCTGTTCCTGGCTAAAATAAATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATTTTATAACggttagt                                                                                                       <  1:1639973/150‑7 (MQ=255)
                                                taGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAg                                                                                                    >  2:1609081/1‑150 (MQ=255)
                                                taGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAg                                                                                                    <  2:371274/150‑1 (MQ=255)
                                                 aGGAATGATTTAACCTGTTCCTGGCTTAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAATAGt                                                                                                   >  2:1362761/1‑150 (MQ=255)
                                                 aGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGt                                                                                                   >  2:1322632/1‑150 (MQ=255)
                                                  ggAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTc                                                                                                  <  2:804645/150‑1 (MQ=255)
                                                  ggAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTc                                                                                                  >  2:552036/1‑150 (MQ=255)
                                                  ggAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTc                                                                                                  >  2:1053186/1‑150 (MQ=255)
                                                   gAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCa                                                                                                 >  1:100799/1‑150 (MQ=255)
                                                    aaTGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCaa                                                                                                >  2:768438/1‑150 (MQ=255)
                                                       gATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTg                                                                                             >  2:1229663/1‑150 (MQ=255)
                                                       gATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTg                                                                                             >  1:645023/1‑150 (MQ=255)
                                                             aCCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATcaca                                                                                       <  1:1571306/150‑1 (MQ=255)
                                                                  ttCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCa                                                                                  <  2:1235791/150‑1 (MQ=255)
                                                                  ttCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCa                                                                                  <  2:845123/150‑1 (MQ=255)
                                                                   tCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaa                                                                                 >  2:1187367/1‑150 (MQ=255)
                                                                    ccTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCaaa                                                                                <  2:1722368/150‑1 (MQ=255)
                                                                      tGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAata                                                                              <  1:1436257/150‑1 (MQ=255)
                                                                         cTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATGTGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCa                                                                           <  2:581612/150‑1 (MQ=255)
                                                                         cTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCa                                                                           <  2:150516/150‑1 (MQ=255)
                                                                           aaaaTACATATAACCGTATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAg                                                                         <  1:599392/150‑1 (MQ=255)
                                                                            aaaTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGt                                                                        <  2:426177/150‑1 (MQ=255)
                                                                             aaTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGtt                                                                       <  1:1672319/150‑1 (MQ=255)
                                                                              aTACATATAAGCGGATGATGACTAATCCATAATACATGTGCGTTTAGTATTGTATCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATATAATAACGTATCAAAAGTCTAGTGATCACATTTCAAATATCAAGTTg                                                                      <  2:446399/150‑1 (MQ=255)
                                                                              aTACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTATTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTAAAGTTATCACATTTCAAATATCAAGttt                                                                      >  1:1068166/1‑149 (MQ=255)
                                                                                     taACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTAt                                                               >  1:1640106/1‑150 (MQ=255)
                                                                                        ccGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTa                                                                <  1:502649/146‑1 (MQ=255)
                                                                                        ccGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTa                                                                >  2:502649/1‑146 (MQ=255)
                                                                                          ggATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTc                                                          <  2:8745/150‑1 (MQ=255)
                                                                                              gatgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGt                                                      <  1:908151/150‑1 (MQ=255)
                                                                                              gatgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGt                                                      <  1:618473/150‑1 (MQ=255)
                                                                                               atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTg                                                     <  2:319807/150‑1 (MQ=255)
                                                                                               atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTg                                                     >  1:298584/1‑150 (MQ=255)
                                                                                               atgaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATAAAGTTGATAGTATTAGTATGGTg                                                     >  1:448218/1‑150 (MQ=255)
                                                                                                 gaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGat                                                   <  2:393949/150‑1 (MQ=255)
                                                                                                 gaCTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGat                                                   >  2:399476/1‑150 (MQ=255)
                                                                                                     aaaCCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGattatt                                               >  2:1574383/1‑150 (MQ=255)
                                                                                                        ccAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGAttatttat                                            >  2:455291/1‑150 (MQ=255)
                                                                                                          aaaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATgg                                          >  2:667366/1‑150 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                         <  1:1104452/150‑1 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                         <  1:1053186/150‑1 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                         <  1:552036/150‑1 (MQ=255)
                                                                                                           aaaTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATggg                                         <  1:646548/150‑1 (MQ=255)
                                                                                                             aTACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTg                                       <  2:98464/150‑1 (MQ=255)
                                                                                                              tACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGa                                      <  2:431083/150‑1 (MQ=255)
                                                                                                               aCATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGAc                                     <  1:368635/150‑1 (MQ=255)
                                                                                                                   gtgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGACTGGTGATTATATATGTGAGACAATa                                 >  2:1643713/1‑150 (MQ=255)
                                                                                                                    tgCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaa                                >  1:300775/1‑150 (MQ=255)
                                                                                                                    tgCGTTAAGTATTGAAACGGACGTGTGGCAAGGCCGTTGTTATTATATATATGTTAACCGTTATAAAATAAAGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTTATAGTATTAGTCTGGTGATTATATATGGGTTACAATaa                                >  1:662966/1‑150 (MQ=255)
                                                                                                                     gCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaa                               >  1:366207/1‑150 (MQ=255)
                                                                                                                      cGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaaa                              >  1:919184/1‑150 (MQ=255)
                                                                                                                       gTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTAAAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATAAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATaaaaa                             >  2:235693/1‑150 (MQ=255)
                                                                                                                        ttAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAg                            <  2:951189/150‑1 (MQ=255)
                                                                                                                          aaGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGAc                          <  1:1391701/150‑1 (MQ=255)
                                                                                                                          aaGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGAc                          <  2:650885/150‑1 (MQ=255)
                                                                                                                          aaGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGAc                          <  2:1648943/150‑1 (MQ=255)
                                                                                                                            taaaaacaaCGGCCGTGCCACACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                        >  2:552636/8‑150 (MQ=255)
                                                                                                                            gTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                        <  2:645023/150‑1 (MQ=255)
                                                                                                                            gTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAg                        <  1:1606241/150‑1 (MQ=255)
                                                                                                                             aaaaacaaCGGCCGTGCCACACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGt                       >  2:990594/7‑150 (MQ=255)
                                                                                                                              aTTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                      >  2:1079016/1‑150 (MQ=255)
                                                                                                                              aTTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTa                      <  1:1148202/150‑1 (MQ=255)
                                                                                                                               ttGAAACGTACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAAGCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTAt                     <  2:855136/150‑1 (MQ=255)
                                                                                                                                tGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTTACCGTTATAAAATAACGTATAAAAAGTAAATTGATCACATTTCAAATATAAAGTTTATATTATTTTTATTGTGATTATTTATGGGTGACAATAAAAACACattatt                    >  2:1550921/1‑150 (MQ=255)
                                                                                                                                 gAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTa                   >  2:1204515/1‑150 (MQ=255)
                                                                                                                                  aaaCGGACGAGTGGCACGGCCGTTGATTTTATAAATATGTAAACCGTTATAAAATAACGAATCAAAAGTCAAGTGATCACATATCAAATATAAAGATGAATCTATTAGTCTCTTGATTATATATGGGTGACAATAAAAAGAAAGTATTaa                  >  1:952/1‑150 (MQ=255)
                                                                                                                                    aCGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAatc                >  2:110173/1‑150 (MQ=255)
                                                                                                                                           tgtgGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGCTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATa         >  2:244544/1‑150 (MQ=255)
                                                                                                                                             tgGCACGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATaga       <  2:727524/150‑1 (MQ=255)
                                                                                                                                              ggCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATagat      >  2:1710200/1‑149 (MQ=255)
                                                                                                                                              ggCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATagag      >  1:469664/1‑150 (MQ=255)
                                                                                                                                               gCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAgaga     >  1:259943/1‑150 (MQ=255)
                                                                                                                                                 aCGTCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGTTGATTATTTATGTGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATa   <  1:48247/150‑1 (MQ=255)
                                                                                                                                                  cGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATACTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAg  <  2:308502/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTATATAGGAATGATTTAACCTGTTCCTGGCTAAAATACATATAACCGGATGATGACTAAACCAAAATACATGTGCGTTAAGTATTGAAACGGACGTGTGGCACGGCCGTTGTTTTTATAAATATGTTAACCGTTATAAAATAACGTATCAAAAGTCAAGTGATCACATTTCAAATATCAAGTTGATAGTATTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAG  >  NC_000913/1212334‑1212629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: