Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,226,114 C→G 6.2% intergenic (‑34/‑486) minC ← / → ycgJ inhibitor of FtsZ ring polymerization/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,226,1140CG 6.2% 288.3 / 9.9 97intergenic (‑34/‑486)minC/ycgJinhibitor of FtsZ ring polymerization/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (52/39);  new base G (4/2);  total (56/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

GCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAACC  >  NC_000913/1225965‑1226263
                                                                                                                                                     |                                                                                                                                                     
gCCTGTGCGATTTTGTCTTCCCTCTCCTGATGGATAACCTTAGGTTCTGCCTCAAGCAGATGAACCACAGAAAAAGTGAAGCTACTTCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGcc                                                                                                                                                       <  1:155217/150‑1 (MQ=255)
   tGAGCGATTTTGTCTTCCAGCGCCTGATGGATACCGTTAGGTTCTGCCTCAGGCAGATGAACCACAGTTAACGTGAAGCTAATGCATTTAAGCACGATTGGCGGTTTTGACAGCGTGGCGTTAGGAAATTAGCTTTTAATCTGCGCCCgc                                                                                                                                                    >  2:1258477/1‑150 (MQ=255)
        gATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGcgcgcg                                                                                                                                               >  1:601930/1‑150 (MQ=255)
        gATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGcgcgcg                                                                                                                                               >  1:1679805/1‑150 (MQ=255)
          ttttGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCgat                                                                                                                                             <  1:1010824/150‑1 (MQ=255)
             tGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatgat                                                                                                                                          <  1:736093/150‑1 (MQ=255)
              gTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCGCGCTGGCgatgatt                                                                                                                                         <  2:720191/150‑2 (MQ=255)
                cTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGtt                                                                                                                                       >  2:406129/1‑150 (MQ=255)
                cTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGAGCGCGATGATGtt                                                                                                                                       >  2:1638632/1‑150 (MQ=255)
                 ttCCAGCGCCTTATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                      <  1:623967/150‑1 (MQ=255)
                 ttCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                      <  2:466192/150‑1 (MQ=255)
                 ttCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTc                                                                                                                                      <  1:1734107/150‑1 (MQ=255)
                  tCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTcc                                                                                                                                     >  2:1029486/1‑150 (MQ=255)
                  tCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTGTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTcc                                                                                                                                     <  2:294063/150‑1 (MQ=255)
                   ccAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGAGTGTTTGACATCATGTCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCg                                                                                                                                    >  1:608516/1‑150 (MQ=255)
                    cAGCGCCTCATTGAAAACCTTCGGTTCTGCCTCATCCAGATGAACCACAGATAAACTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTGGACATCCTGGCCTTACTCAATTCGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGa                                                                                                                                   <  1:825667/150‑1 (MQ=255)
                     aGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaa                                                                                                                                  >  2:496888/1‑150 (MQ=255)
                     aGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGaa                                                                                                                                  >  1:454606/1‑150 (MQ=255)
                     aGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAAACACACATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATACTGGCCTAACTCAATTAGCTATTAATCATCGCCAGCGCGAGATGATGTTCCGaa                                                                                                                                  >  2:460082/1‑150 (MQ=255)
                        gcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGAc                                                                                                                               <  1:1611800/150‑1 (MQ=255)
                        gcCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGAc                                                                                                                               <  1:1254655/150‑1 (MQ=255)
                          cTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACGGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACta                                                                                                                             >  2:1137368/1‑150 (MQ=255)
                           tGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACtat                                                                                                                            >  2:1723469/1‑150 (MQ=255)
                           tGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACtat                                                                                                                            >  1:651935/1‑150 (MQ=255)
                             aTGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACGGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATaa                                                                                                                          <  1:1137368/150‑1 (MQ=255)
                             aTGGATAACCTTAGGTTCTGCCTCATGCAGATGAACAACAGATAAAGTTATGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATaa                                                                                                                          <  2:236685/150‑1 (MQ=255)
                              tGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAg                                                                                                                         <  1:594160/150‑1 (MQ=255)
                                gATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGc                                                                                                                       >  1:1301526/1‑150 (MQ=255)
                                 aTAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCa                                                                                                                      <  1:1486043/150‑1 (MQ=255)
                                 aTAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCa                                                                                                                      <  1:1406008/150‑1 (MQ=255)
                                    aCCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGt                                                                                                                   >  1:606931/1‑150 (MQ=255)
                                    aCCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGATTGACATCATGTCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGt                                                                                                                   >  1:1551483/1‑150 (MQ=255)
                                     ccTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGtt                                                                                                                  >  2:871478/1‑150 (MQ=255)
                                      cTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTta                                                                                                                 >  1:353125/1‑150 (MQ=255)
                                                gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                       >  2:987969/1‑150 (MQ=255)
                                                gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                       >  1:1582755/1‑150 (MQ=255)
                                                gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                       >  1:58773/1‑150 (MQ=255)
                                                gCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGAt                                                                                                       >  2:1718902/1‑150 (MQ=255)
                                                    cATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATtata                                                                                                   >  1:704371/1‑150 (MQ=255)
                                                      tGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTTATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATAtt                                                                                                 <  1:1550390/150‑1 (MQ=255)
                                                        cAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGAAGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGa                                                                                               <  2:601930/150‑1 (MQ=255)
                                                         aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAg                                                                                              <  2:1413490/150‑1 (MQ=255)
                                                         aGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAg                                                                                              >  1:491363/1‑150 (MQ=255)
                                                          gATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAgg                                                                                             <  2:39495/150‑1 (MQ=255)
                                                               aCCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGt                                                                                        >  2:430514/1‑150 (MQ=255)
                                                                  acaGATAAAGTGAAGCTACTGCCTTTACGCTCGATTGGCGTTTTTGACATCCTGGCCTTACTCAATTAGCTATTAATAATCGCCAGCGCGCGATGATGTTCCGAAGACTATAATGCATGTTATAGTCTGGATTATATTGAGGCAAGTCAc                                                                                     >  1:85641/1‑150 (MQ=255)
                                                                  acaGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCAc                                                                                     <  2:454606/150‑1 (MQ=255)
                                                                     gATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCATGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACACt                                                                                  >  1:1020764/1‑150 (MQ=255)
                                                                      aTAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTc                                                                                 <  1:1666728/150‑1 (MQ=255)
                                                                       tAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGAGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCTGCGCGCGATGATGTTCCGAAGACTTTAAGGCATGTTATAGTCTGGATTATTTTGAGGCAAGTCACCCTcc                                                                                <  2:755650/150‑1 (MQ=255)
                                                                         aaGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCa                                                                              >  1:406028/1‑150 (MQ=255)
                                                                         aaGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCa                                                                              >  1:1672949/1‑150 (MQ=255)
                                                                          aGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTTTAGTCTGGATTATATTGAGGCAAGTCGCCCTCCCAt                                                                             >  1:868321/1‑150 (MQ=255)
                                                                          aGTGAAGCAACTGCCTTTAAGCTCGATGGGCGTGTATGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGGCTGGATTATATTGCGGCAAGTCCCCCTCCCAt                                                                             >  2:1210522/1‑150 (MQ=255)
                                                                            tGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCAttt                                                                           <  2:754508/150‑1 (MQ=255)
                                                                             gAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTa                                                                          >  1:202233/1‑150 (MQ=255)
                                                                              aaGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAt                                                                         >  2:1625885/1‑150 (MQ=255)
                                                                               aGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTAtt                                                                        >  1:1402223/1‑150 (MQ=255)
                                                                                gCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTc                                                                       <  2:1413438/150‑1 (MQ=255)
                                                                                gCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTc                                                                       <  1:756822/150‑1 (MQ=255)
                                                                                  tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCATTTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCag                                                                     <  2:608516/150‑1 (MQ=255)
                                                                                  tACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGATCCGAAGACTATAAGGCAGGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCag                                                                     <  1:195466/150‑1 (MQ=255)
                                                                                   aCTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCaga                                                                    >  2:344462/1‑150 (MQ=255)
                                                                                    cTGCCTTTACGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCagag                                                                   <  2:828168/150‑1 (MQ=255)
                                                                                    cTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCagag                                                                   <  2:295409/150‑1 (MQ=255)
                                                                                        cTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTaaa                                                               >  2:1366417/1‑150 (MQ=255)
                                                                                           tAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAATTCACCCTCCCATTTATTCAGAGTAAAAGt                                                            >  1:1242946/1‑150 (MQ=255)
                                                                                            aaGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTc                                                           >  1:731272/1‑150 (MQ=255)
                                                                                             aGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCt                                                          >  1:860227/1‑150 (MQ=255)
                                                                                                      ggCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGAt                                                >  1:946840/1‑150 (MQ=255)
                                                                                                         gtgtTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTATGTGATaa                                              >  1:955154/1‑150 (MQ=255)
                                                                                                          tgtTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATaaa                                             >  1:354737/1‑150 (MQ=255)
                                                                                                            tttGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATg                                           <  1:4379/150‑1 (MQ=255)
                                                                                                              tGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgc                                         <  2:606931/150‑1 (MQ=255)
                                                                                                              tGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgc                                         <  1:146604/150‑1 (MQ=255)
                                                                                                               gACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgcg                                        <  2:873091/150‑1 (MQ=255)
                                                                                                                aCATCCTGGCCTTACTCAATTAGCAATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGgcgc                                       <  1:1546091/150‑1 (MQ=255)
                                                                                                                 cATCCTGGCCTTACTCAATAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTg                                     <  2:20111/150‑1 (MQ=255)
                                                                                                                    ccTGGCCTTACTCAATTATATATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGAt                                   <  1:708888/150‑1 (MQ=255)
                                                                                                                            tACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCt                           >  1:1418390/1‑150 (MQ=255)
                                                                                                                            tACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCt                           >  1:1336348/1‑150 (MQ=255)
                                                                                                                              cTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTa                         <  2:58773/150‑1 (MQ=255)
                                                                                                                                cAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaaa                       >  2:421086/1‑150 (MQ=255)
                                                                                                                                cAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTACCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaaa                       >  2:40604/1‑150 (MQ=255)
                                                                                                                                  aTTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTaaaaa                     >  2:1444288/1‑150 (MQ=255)
                                                                                                                                      gCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAc                 <  1:496888/150‑1 (MQ=255)
                                                                                                                                       cggaacatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAcc                >  2:669796/7‑150 (MQ=255)
                                                                                                                                        tATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAccc               >  2:576283/1‑150 (MQ=255)
                                                                                                                                           tAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTg            >  2:944529/1‑150 (MQ=255)
                                                                                                                                           acatcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTCTATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTg            >  2:1340615/3‑150 (MQ=255)
                                                                                                                                            catcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTATATTCTGTGATAAATGTCGCTGATTAATATCTTAAAAAATAACCTTGt           >  1:324503/2‑150 (MQ=255)
                                                                                                                                            aaTCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGt           <  2:1679805/150‑1 (MQ=255)
                                                                                                                                             atcatcGCGCGCTGGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGta          >  2:1690302/1‑149 (MQ=255)
                                                                                                                                             atcatcGCCAGCGCGCGATGATGTTACGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtc          <  1:611750/150‑1 (MQ=255)
                                                                                                                                                atcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaat       >  1:921468/1‑150 (MQ=255)
                                                                                                                                                atcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaat       >  1:794020/1‑150 (MQ=255)
                                                                                                                                                atcGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaat       >  2:693175/1‑150 (MQ=255)
                                                                                                                                                   gCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGtcaatcaa    <  1:1029486/150‑1 (MQ=255)
                                                                                                                                                    ccAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATAACCTTTTCAATAAAc   >  1:918692/1‑150 (MQ=255)
                                                                                                                                                     cAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAAcc  <  2:353125/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGAGCGATTTTGTCTTCCAGCGCCTGATGGATAACCTTAGGTTCTGCCTCATGCAGATGAACCACAGATAAAGTGAAGCTACTGCCTTTAAGCTCGATTGGCGTGTTTGACATCCTGGCCTTACTCAATTAGCTATTAATCATCGCCAGCGCGCGATGATGTTCCGAAGACTATAAGGCATGTTATAGTCTGGATTATATTGAGGCAAGTCACCCTCCCATTTATTCAGAGTAAAAGTCTATTCTGTGATAAATGGCGCTGATTCATAGCTTAAAAAATACCCTTGTCAATCAACC  >  NC_000913/1225965‑1226263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: