Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 9.6% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT 9.6% 297.9 / 13.6 103intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (58/35);  new base T (7/3);  total (65/38)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 107 total aligned reads displayed.

AAAGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACT  >  NC_000913/1232332‑1232630
                                                                                                                                                     |                                                                                                                                                      
aaaGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAATAAAGCGCTccc                                                                                                                                                        >  2:1104595/1‑150 (MQ=255)
 aaGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCg                                                                                                                                                       <  2:1104458/150‑1 (MQ=255)
  agagGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGc                                                                                                                                                      >  1:1469773/1‑150 (MQ=255)
  agagGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGc                                                                                                                                                      >  2:1480522/1‑150 (MQ=255)
        cAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCCCCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgc                                                                                                                                                >  2:114338/1‑150 (MQ=255)
         agagGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                                                                               >  1:1437313/1‑150 (MQ=255)
           agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  2:1529213/1‑150 (MQ=255)
           agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  2:912781/1‑150 (MQ=255)
           agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  1:517513/1‑150 (MQ=255)
            ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAACAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            >  2:1348959/1‑150 (MQ=255)
            ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            >  1:1601787/1‑150 (MQ=255)
            ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            <  2:229250/150‑1 (MQ=255)
              aaCACTCTATTTTGCCGGGCAGGGTATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCGGATTTACTGAGGGGCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                                                                          <  1:468657/150‑5 (MQ=255)
                cacTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttt                                                                                                                                        <  1:308310/150‑7 (MQ=255)
                 acTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                                                                       <  1:555600/150‑1 (MQ=255)
                  ctctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATggt                                                                                                                                      >  2:1099610/1‑149 (MQ=255)
                  ctctATTTTGCCGGGCAGGGGATCCAGCAACAAAGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                      >  1:1025971/1‑150 (MQ=255)
                    ctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCCCCACGGTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGCGCGCCGAATGGAtt                                                                                                                                    >  2:5326/1‑150 (MQ=255)
                          tGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGACGAATGGATTAGCGAc                                                                                                                              >  1:671491/1‑150 (MQ=255)
                            ccGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCCCGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCg                                                                                                                            >  1:1039690/1‑150 (MQ=255)
                             cGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa                                                                                                                           >  1:709737/1‑150 (MQ=255)
                                  aGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGATAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                                                                      >  2:61232/1‑150 (MQ=255)
                                  aGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                                                                      <  1:1393163/150‑1 (MQ=255)
                                   ggggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAt                                                                                                                     <  2:15796/150‑1 (MQ=255)
                                    gggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                                                                                    <  2:633795/150‑1 (MQ=255)
                                      gATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGTGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAc                                                                                                                  <  2:866015/150‑1 (MQ=255)
                                        tCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGt                                                                                                               >  1:541979/1‑150 (MQ=255)
                                          cAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttt                                                                                                             >  2:783437/1‑150 (MQ=255)
                                            gCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttttt                                                                                                           >  2:792967/1‑150 (MQ=255)
                                              aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                         >  2:824935/1‑150 (MQ=255)
                                              aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                         >  1:1042557/1‑150 (MQ=255)
                                                  aTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                           >  2:738274/1‑110 (MQ=255)
                                                    ggCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                                                                   >  2:677815/1‑150 (MQ=255)
                                                       ataTGAAGGGATCGATGCTTTCATAACGTTATAAAACGCTAAGTTCTGATTTACTGAGGGTCAAATAAATATAGGGGCAGGTAAAAAGCGCTCCCGCAGGTGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                                                                                <  2:1167445/148‑1 (MQ=255)
                                                       aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAATGATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGCCCGAACAGATCACGTTTTTTCGCTTTAAAc                                                                                                >  2:1043495/1‑150 (MQ=255)
                                                         aTGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACgg                                                                                              >  1:1353158/1‑150 (MQ=255)
                                                           gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGATGGTCAAATAAATATAGCGGCAGGGAAAAAGCGCCCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCt                                                                                            <  2:980925/150‑1 (MQ=255)
                                                            aaGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                                                                           <  2:1437313/150‑1 (MQ=255)
                                                              gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                                                                         <  2:1482535/150‑1 (MQ=255)
                                                                 aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa                                                                                      <  2:1601787/150‑1 (MQ=255)
                                                                   ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTTCTGAGGGTCAAATAAATATAGCGGCAGGAAAACAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                                                                    <  2:1053576/150‑1 (MQ=255)
                                                                   ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                                                                    <  2:218204/150‑1 (MQ=255)
                                                                    cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGCACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAACTcc                                                                                   >  2:293878/1‑149 (MQ=255)
                                                                      tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                                                                                 <  1:677815/150‑1 (MQ=255)
                                                                          ttCACCACGTTATACAACGAGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGATCCCGCAGTAGCGCCGAATGGATTAGCGACAGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                                                                             >  1:524600/1‑150 (MQ=255)
                                                                           tCACCACGTTCTACAACGCGACGTTCTGATTTACTGAGGGTCAACAAAATATAGCGGCAGGAAAAAAGCGCGCACGCAGGAGCGCCGAAGGGATTAGCGACCGAACAGCTCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAg                                                                            <  2:1102202/150‑1 (MQ=255)
                                                                             accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:658504/1‑150 (MQ=255)
                                                                             accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:1624606/1‑150 (MQ=255)
                                                                             accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:822235/1‑150 (MQ=255)
                                                                              ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAc                                                                         <  1:1326136/150‑1 (MQ=255)
                                                                                    tataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                                                                   <  2:1611922/150‑1 (MQ=255)
                                                                                      taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa                                                                 >  2:1550346/1‑150 (MQ=255)
                                                                                       aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCAGAATGGATTAGAGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt                                                                >  1:120036/1‑150 (MQ=255)
                                                                                        caccgcgCAGGTCTGATTTACAGAGGGACAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                               <  1:1435522/147‑1 (MQ=255)
                                                                                         aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                              >  1:464363/1‑150 (MQ=255)
                                                                                         aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                              <  1:1230758/150‑1 (MQ=255)
                                                                                          aCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCCCTGCGACAATCAg                                                             >  1:1099837/1‑150 (MQ=255)
                                                                                           cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  2:517513/150‑1 (MQ=255)
                                                                                           cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  2:822235/150‑1 (MQ=255)
                                                                                           cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  1:714297/150‑1 (MQ=255)
                                                                                            gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt                                                           >  1:1080930/1‑150 (MQ=255)
                                                                                            gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt                                                           >  1:99255/1‑150 (MQ=255)
                                                                                               aaGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg                                                        <  1:1529213/150‑1 (MQ=255)
                                                                                                aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc                                                       >  1:15472/1‑150 (MQ=255)
                                                                                                 gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg                                                      >  2:1286270/1‑150 (MQ=255)
                                                                                                 gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg                                                      >  1:1341891/1‑150 (MQ=255)
                                                                                                     tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAAAGGATTAGCGACCGAACAGATCACGTTTTTTCTCTTTAAACGGCTGGGAAAACCCCACCCGCACTGCGACAATCAGGTAAGAGATaa                                                  >  1:1666308/1‑150 (MQ=255)
                                                                                                          tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCCCCACCAGCACGGCGACAATCAGGTAAGCGATAAAAATa                                             >  2:859530/1‑150 (MQ=255)
                                                                                                             ggAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCg                                          <  1:293878/149‑1 (MQ=255)
                                                                                                                  gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagcag                                     <  2:1403992/150‑1 (MQ=255)
                                                                                                                  gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagcag                                     >  1:95331/1‑150 (MQ=255)
                                                                                                                    cAAATAAATATAGCGGCAGGAAAAAAGCGTTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTACGCTTAAAACGCCTGGGAAATCACCACCAGCACTCCGACAATCAGGTAAGCGATAAAAATACCGacacccc                                   >  2:392789/1‑144 (MQ=255)
                                                                                                                    cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                   <  2:682745/150‑1 (MQ=255)
                                                                                                                    cAAATAAATATAGCGGCAGGAAAAAAGAGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCCCCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACAGAGCAGcc                                   >  1:526246/1‑150 (MQ=255)
                                                                                                                      aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                                 >  1:489913/1‑150 (MQ=255)
                                                                                                                      aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                                 <  1:1480522/150‑1 (MQ=255)
                                                                                                                        taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTg                               >  2:1131997/1‑150 (MQ=255)
                                                                                                                          aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCg                             <  1:1725824/150‑1 (MQ=255)
                                                                                                                                gCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCAttt                       >  1:991707/1‑150 (MQ=255)
                                                                                                                                 cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTc                      <  1:824935/150‑1 (MQ=255)
                                                                                                                                  ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTGTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                     >  1:287942/1‑150 (MQ=255)
                                                                                                                                    cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                   >  2:1525962/1‑150 (MQ=255)
                                                                                                                                      ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTCGCTTAAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGa                  >  2:1006327/1‑150 (MQ=255)
                                                                                                                                      ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGTGGCATTTCCAGAc                 >  1:427350/1‑150 (MQ=255)
                                                                                                                                      ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAc                 >  2:50489/1‑150 (MQ=255)
                                                                                                                                       gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                >  2:612512/1‑150 (MQ=255)
                                                                                                                                       gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                <  1:783437/150‑1 (MQ=255)
                                                                                                                                       gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                <  2:1328352/150‑1 (MQ=255)
                                                                                                                                        cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGGCCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCCCCCGCACTGCGACAATCAGGTACGCGATAAAAATCCCGAGCAGCCACTGCGGCATGTCCAGACCt               >  1:1545160/7‑150 (MQ=255)
                                                                                                                                        cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCCGCCACTGCGGCATTTCCAGACCt               >  2:1220154/7‑150 (MQ=255)
                                                                                                                                        aaaaaaGCGCTCCCGCAGGAGCGCCGTATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGTATTGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCTGCATTTCCAGACCt               <  2:685149/150‑1 (MQ=255)
                                                                                                                                         attcggcgcTCCTGCGGGAGCGCTGAATGGATTAGCGACCGAACAGATCATGTTTTTTCGCTTTAAACGGCTGTGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAAACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  1:913665/6‑150 (MQ=255)
                                                                                                                                         attcggcgcTCCTGCGGGAGCGCTGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  1:1435492/6‑150 (MQ=255)
                                                                                                                                         aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  2:563526/1‑150 (MQ=255)
                                                                                                                                            cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  2:1385801/3‑150 (MQ=255)
                                                                                                                                            aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  1:845946/1‑150 (MQ=255)
                                                                                                                                            aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  1:1648836/1‑150 (MQ=255)
                                                                                                                                               cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAAt        >  1:1444633/1‑150 (MQ=255)
                                                                                                                                                     cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGTTGGGAAATCACAACCAGCACTGCGACAAGCAGGTAAGCGATAAAAATACCGAGCCGACACTGCGGCATTTCCAGACCTAAAAAATCCCACt  >  2:827921/1‑150 (MQ=255)
                                                                                                                                                     cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACt  >  1:187/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAAGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACT  >  NC_000913/1232332‑1232630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: