Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,484 A→G 9.8% intergenic (+30/+16) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4840AG 9.8% 275.4 / 17.9 102intergenic (+30/+16)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base A (58/34);  new base G (7/3);  total (65/37)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01

Only 100 of 102 total aligned reads displayed.

CAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACT  >  NC_000913/1232340‑1232630
                                                                                                                                                |                                                                                                                                                   
cAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCCCCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgc                                                                                                                                                >  2:114338/1‑150 (MQ=255)
 agagGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAgcg                                                                                                                                               >  1:1437313/1‑150 (MQ=255)
   agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  2:1529213/1‑150 (MQ=255)
   agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  1:517513/1‑150 (MQ=255)
   agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                             >  2:912781/1‑150 (MQ=255)
    ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAACAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            >  2:1348959/1‑150 (MQ=255)
    ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            >  1:1601787/1‑150 (MQ=255)
    ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                                                                            <  2:229250/150‑1 (MQ=255)
      aaCACTCTATTTTGCCGGGCAGGGTATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCGGATTTACTGAGGGGCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttt                                                                                                                                          <  1:468657/150‑5 (MQ=255)
        cacTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgctttttt                                                                                                                                        <  1:308310/150‑7 (MQ=255)
         acTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATgg                                                                                                                                       <  1:555600/150‑1 (MQ=255)
          ctctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATggt                                                                                                                                      >  2:1099610/1‑149 (MQ=255)
          ctctATTTTGCCGGGCAGGGGATCCAGCAACAAAGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                      >  1:1025971/1‑150 (MQ=255)
            ctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCCCCACGGTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGCGCGCCGAATGGAtt                                                                                                                                    >  2:5326/1‑150 (MQ=255)
                  tGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGACGAATGGATTAGCGAc                                                                                                                              >  1:671491/1‑150 (MQ=255)
                    ccGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCCCGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCg                                                                                                                            >  1:1039690/1‑150 (MQ=255)
                     cGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa                                                                                                                           >  1:709737/1‑150 (MQ=255)
                          aGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGATAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                                                                      >  2:61232/1‑150 (MQ=255)
                          aGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGa                                                                                                                      <  1:1393163/150‑1 (MQ=255)
                           ggggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAt                                                                                                                     <  2:15796/150‑1 (MQ=255)
                            gggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATc                                                                                                                    <  2:633795/150‑1 (MQ=255)
                              gATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGTGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAc                                                                                                                  <  2:866015/150‑1 (MQ=255)
                                tCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGt                                                                                                               >  1:541979/1‑150 (MQ=255)
                                  cAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttt                                                                                                             >  2:783437/1‑150 (MQ=255)
                                    gCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTATCGACCGAACAGATCACGttttt                                                                                                           >  1:364619/1‑150 (MQ=255)
                                    gCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttttt                                                                                                           <  2:1109362/150‑1 (MQ=255)
                                    gCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttttt                                                                                                           >  2:792967/1‑150 (MQ=255)
                                      aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                         >  2:824935/1‑150 (MQ=255)
                                      aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                         >  1:1042557/1‑150 (MQ=255)
                                          aTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                           >  2:738274/1‑110 (MQ=255)
                                          aTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                           <  1:738274/113‑4 (MQ=255)
                                            ggCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTa                                                                                                   >  2:677815/1‑150 (MQ=255)
                                               ataTGAAGGGATCGATGCTTTCATAACGTTATAAAACGCTAAGTTCTGATTTACTGAGGGTCAAATAAATATAGGGGCAGGTAAAAAGCGCTCCCGCAGGTGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                                                                                <  2:1167445/148‑1 (MQ=255)
                                               aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAATGATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGCCCGAACAGATCACGTTTTTTCGCTTTAAAc                                                                                                >  2:1043495/1‑150 (MQ=255)
                                                 aTGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACgg                                                                                              >  1:1353158/1‑150 (MQ=255)
                                                   gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGATGGTCAAATAAATATAGCGGCAGGGAAAAAGCGCCCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCt                                                                                            <  2:980925/150‑1 (MQ=255)
                                                    aaGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                                                                           <  2:1437313/150‑1 (MQ=255)
                                                      gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTggg                                                                                         <  2:1482535/150‑1 (MQ=255)
                                                         aGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGaaa                                                                                      <  2:1601787/150‑1 (MQ=255)
                                                           ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTTCTGAGGGTCAAATAAATATAGCGGCAGGAAAACAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                                                                    <  2:1053576/150‑1 (MQ=255)
                                                           ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                                                                    <  2:218204/150‑1 (MQ=255)
                                                            cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGCACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAACTcc                                                                                   >  2:293878/1‑149 (MQ=255)
                                                              tGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATcacc                                                                                 <  1:677815/150‑1 (MQ=255)
                                                                  ttCACCACGTTATACAACGAGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGATCCCGCAGTAGCGCCGAATGGATTAGCGACAGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCAccacca                                                                             >  1:524600/1‑150 (MQ=255)
                                                                   tCACCACGTTCTACAACGCGACGTTCTGATTTACTGAGGGTCAACAAAATATAGCGGCAGGAAAAAAGCGCGCACGCAGGAGCGCCGAAGGGATTAGCGACCGAACAGCTCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAg                                                                            <  2:1102202/150‑1 (MQ=255)
                                                                     accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:822235/1‑150 (MQ=255)
                                                                     accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:1624606/1‑150 (MQ=255)
                                                                     accacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCa                                                                          >  1:658504/1‑150 (MQ=255)
                                                                      ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAc                                                                         <  1:1326136/150‑1 (MQ=255)
                                                                            tataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                                                                   <  2:1611922/150‑1 (MQ=255)
                                                                              taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa                                                                 >  2:1550346/1‑150 (MQ=255)
                                                                               aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCAGAATGGATTAGAGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt                                                                >  1:120036/1‑150 (MQ=255)
                                                                                caccgcgCAGGTCTGATTTACAGAGGGACAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                               <  1:1435522/147‑1 (MQ=255)
                                                                                 aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                              <  1:1230758/150‑1 (MQ=255)
                                                                                 aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                              >  1:464363/1‑150 (MQ=255)
                                                                                  aCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCCCTGCGACAATCAg                                                             >  1:1099837/1‑150 (MQ=255)
                                                                                   cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  1:714297/150‑1 (MQ=255)
                                                                                   cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  2:822235/150‑1 (MQ=255)
                                                                                   cgcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAgg                                                            <  2:517513/150‑1 (MQ=255)
                                                                                    gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt                                                           >  1:99255/1‑150 (MQ=255)
                                                                                    gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt                                                           >  1:1080930/1‑150 (MQ=255)
                                                                                       aaGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg                                                        <  1:1529213/150‑1 (MQ=255)
                                                                                        aGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGc                                                       >  1:15472/1‑150 (MQ=255)
                                                                                         gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg                                                      >  1:1341891/1‑150 (MQ=255)
                                                                                         gTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCg                                                      >  2:1286270/1‑150 (MQ=255)
                                                                                             tGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAAAGGATTAGCGACCGAACAGATCACGTTTTTTCTCTTTAAACGGCTGGGAAAACCCCACCCGCACTGCGACAATCAGGTAAGAGATaa                                                  >  1:1666308/1‑150 (MQ=255)
                                                                                                  tACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCCCCACCAGCACGGCGACAATCAGGTAAGCGATAAAAATa                                             >  2:859530/1‑150 (MQ=255)
                                                                                                   aCTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAc                                            >  2:623555/1‑150 (MQ=255)
                                                                                                     ggAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCg                                          <  1:293878/149‑1 (MQ=255)
                                                                                                          gTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagcag                                     <  2:1403992/150‑1 (MQ=255)
                                                                                                            cAAATAAATATAGCGGCAGGAAAAAAGCGTTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTACGCTTAAAACGCCTGGGAAATCACCACCAGCACTCCGACAATCAGGTAAGCGATAAAAATACCGacacccc                                   >  2:392789/1‑144 (MQ=255)
                                                                                                            cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                   <  2:682745/150‑1 (MQ=255)
                                                                                                            cAAATAAATATAGCGGCAGGAAAAAAGAGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCCCCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACAGAGCAGcc                                   >  1:526246/1‑150 (MQ=255)
                                                                                                              aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                                 >  1:489913/1‑150 (MQ=255)
                                                                                                              aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                                 <  1:1480522/150‑1 (MQ=255)
                                                                                                                taaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTg                               >  2:1131997/1‑150 (MQ=255)
                                                                                                                  aataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCg                             <  1:1725824/150‑1 (MQ=255)
                                                                                                                        gCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCAttt                       >  1:991707/1‑150 (MQ=255)
                                                                                                                         cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTc                      <  1:824935/150‑1 (MQ=255)
                                                                                                                          ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTGTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                     >  1:287942/1‑150 (MQ=255)
                                                                                                                            cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                   >  2:1525962/1‑150 (MQ=255)
                                                                                                                              ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTTCGCTTAAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGa                  >  2:1006327/1‑150 (MQ=255)
                                                                                                                              ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGTGGCATTTCCAGAc                 >  1:427350/1‑150 (MQ=255)
                                                                                                                              ggAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAc                 >  2:50489/1‑150 (MQ=255)
                                                                                                                               gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                <  1:783437/150‑1 (MQ=255)
                                                                                                                               gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                >  2:612512/1‑150 (MQ=255)
                                                                                                                               gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc                <  2:1328352/150‑1 (MQ=255)
                                                                                                                                cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGGCCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCCCCCGCACTGCGACAATCAGGTACGCGATAAAAATCCCGAGCAGCCACTGCGGCATGTCCAGACCt               >  1:1545160/7‑150 (MQ=255)
                                                                                                                                cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCCGCCACTGCGGCATTTCCAGACCt               >  2:1220154/7‑150 (MQ=255)
                                                                                                                                aaaaaaGCGCTCCCGCAGGAGCGCCGTATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGTATTGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCTGCATTTCCAGACCt               <  2:685149/150‑1 (MQ=255)
                                                                                                                                 attcggcgcTCCTGCGGGAGCGCTGAATGGATTAGCGACCGAACAGATCATGTTTTTTCGCTTTAAACGGCTGTGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAAAACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  1:913665/6‑150 (MQ=255)
                                                                                                                                 attcggcgcTCCTGCGGGAGCGCTGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  1:1435492/6‑150 (MQ=255)
                                                                                                                                 aaaaaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa              >  2:563526/1‑150 (MQ=255)
                                                                                                                                    cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  1:1466898/3‑150 (MQ=255)
                                                                                                                                    cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  2:1385801/3‑150 (MQ=255)
                                                                                                                                    aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  1:1648836/1‑150 (MQ=255)
                                                                                                                                    aaGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaaa           >  1:845946/1‑150 (MQ=255)
                                                                                                                                       cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAAt        >  1:1444633/1‑150 (MQ=255)
                                                                                                                                             cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGTTGGGAAATCACAACCAGCACTGCGACAAGCAGGTAAGCGATAAAAATACCGAGCCGACACTGCGGCATTTCCAGACCTAAAAAATCCCACt  >  2:827921/1‑150 (MQ=255)
                                                                                                                                             cGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACt  >  1:187/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCACT  >  NC_000913/1232340‑1232630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: