Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,248,561 G→A 12.1% A185V (GCC→GTC)  dhaM ← putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,248,5610GA12.1% 241.2 / 17.4 92A185V (GCC→GTC) dhaMputative dihydroxyacetone‑specific PTS enzymes: HPr, EI components
Reads supporting (aligned to +/- strand):  ref base G (40/39);  new base A (4/7);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCC  >  NC_000913/1248413‑1248710
                                                                                                                                                    |                                                                                                                                                     
cagcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGc                                                                                                                                                      <  1:716593/150‑1 (MQ=255)
  gcTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGACGTTTTTTTCCAGCAACATATCGGCAt                                                                                                                                                    >  1:1173367/1‑150 (MQ=255)
    tGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTa                                                                                                                                                  >  2:346129/1‑150 (MQ=255)
    tGACGGAAAGCGAGCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTa                                                                                                                                                  <  1:1266223/150‑1 (MQ=255)
     gACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTaa                                                                                                                                                 >  2:120157/1‑150 (MQ=255)
      aCGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTaaa                                                                                                                                                <  1:155294/150‑1 (MQ=255)
       cGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATTCTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATAGGCATTAAAt                                                                                                                                               >  2:286689/1‑150 (MQ=255)
       cGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACACATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAAt                                                                                                                                               >  1:241846/1‑150 (MQ=255)
         gAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATgc                                                                                                                                             <  1:1726298/150‑2 (MQ=255)
          aaaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTc                                                                                                                                            <  1:437974/150‑1 (MQ=255)
             gCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGAt                                                                                                                                         >  2:732464/1‑150 (MQ=255)
              cGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATa                                                                                                                                        <  2:588145/150‑1 (MQ=255)
               gATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATaa                                                                                                                                       >  1:1694804/1‑150 (MQ=255)
               gATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATat                                                                                                                                       <  1:760024/150‑2 (MQ=255)
                aTCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCAGCAACATATCGGCATTAAATGTCGATAAgg                                                                                                                                     <  1:13301/150‑1 (MQ=255)
                 tCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATAATTCGCATCGTTTCGTTATAGCGAACTTGTAGTATAGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAgg                                                                                                                                     <  1:195983/150‑1 (MQ=255)
                 tCAGTGCCTCATCAGAATCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGAGTAATTGTAGTAACTCAATCTGTTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTACCAGCAACATATCGACATTTAATGCCGATatgt                                                                                                                                     <  2:227050/150‑4 (MQ=255)
                   aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtt                                                                                                                                   >  2:868878/1‑149 (MQ=255)
                   aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATatgttg                                                                                                                                   >  2:165193/1‑145 (MQ=255)
                    gTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtat                                                                                                                                  >  2:1126718/1‑150 (MQ=255)
                     tGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtata                                                                                                                                 <  1:104219/150‑1 (MQ=255)
                     tGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATatgttgct                                                                                                                                 <  2:936297/150‑8 (MQ=255)
                        ctctTCAGCTTCTGGCGCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCACTGGTGTGACGCATTTGCCGTTTTTTTCCAGCATCATATCGGCATTAAATGTCGATAAGGTATAAAc                                                                                                                              <  2:337293/150‑1 (MQ=255)
                         tctTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCTAACTTGAAGTAACGCAATCTGGTTAATACTCTCTGGTGTTACGCATTTGCCGTTTTTTTCCAGCAACATCTCGGCATTAAATGTCGATAAGGTATAAAcc                                                                                                                             <  1:337848/150‑1 (MQ=255)
                          ctTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTACAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCa                                                                                                                            >  2:1401461/1‑150 (MQ=255)
                           ttCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCac                                                                                                                           >  2:1275236/1‑149 (MQ=255)
                             cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGcc                                                                                                                         >  2:821471/1‑150 (MQ=255)
                               gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCgg                                                                                                                       <  1:623804/150‑1 (MQ=255)
                                       cccTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCgg                                                                                                               >  1:1120346/1‑150 (MQ=255)
                                        ccTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGa                                                                                                              <  1:732464/150‑1 (MQ=255)
                                         cTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGAc                                                                                                             <  2:1184119/150‑1 (MQ=255)
                                         cTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGAc                                                                                                             <  2:643552/150‑1 (MQ=255)
                                          tttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACg                                                                                                            <  1:55425/150‑1 (MQ=255)
                                           ttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGt                                                                                                           >  2:1375175/1‑150 (MQ=255)
                                           ttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGt                                                                                                           >  2:1368447/1‑150 (MQ=255)
                                             cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGTCATTAAATGTCGATAAGGTTTAAAACATCCGGGAGGCCGGACGTaa                                                                                                         >  1:1216326/1‑149 (MQ=255)
                                             cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGTCCGGACGTAc                                                                                                         >  1:213357/1‑150 (MQ=255)
                                             cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAAAACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTAAAACCCAGCCGGGAGGCCGGACGTAc                                                                                                         >  1:156536/1‑150 (MQ=255)
                                                 aTCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGc                                                                                                     >  1:428138/1‑150 (MQ=255)
                                                     ggCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGc                                                                                                 <  1:1654223/150‑1 (MQ=255)
                                                       cgcAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCg                                                                                               >  2:1495569/1‑150 (MQ=255)
                                                        gtaGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATAGTCATTAAATGTAGATAAGGTATAAACCAGCCGGGAGGCAGGACGTACATGCAGGCCGt                                                                                              >  1:727184/3‑150 (MQ=255)
                                                         cAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGtt                                                                                             >  2:210861/1‑150 (MQ=255)
                                                         cAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGtt                                                                                             <  1:1265589/150‑1 (MQ=255)
                                                                 cGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGtttt                                                                                     <  1:346129/150‑1 (MQ=255)
                                                                  gTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTGACGGttttt                                                                                    >  2:1222824/1‑150 (MQ=255)
                                                                   ttATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGtttttt                                                                                   <  2:1200753/150‑1 (MQ=255)
                                                                    tataGCGAACTTGTAGTAACGCAATCTGGGTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTACATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTa                                                                                  <  1:1087139/150‑1 (MQ=255)
                                                                        gCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATTTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGac                                                                              >  2:1516895/1‑150 (MQ=255)
                                                                        gCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGac                                                                              >  2:1466378/1‑150 (MQ=255)
                                                                         cGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGacc                                                                             <  1:1691306/150‑1 (MQ=255)
                                                                         cGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGacc                                                                             <  2:1120346/150‑1 (MQ=255)
                                                                               tGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCAGTTACGGTTTTTTATGACCACCGcc                                                                       >  1:198882/1‑150 (MQ=255)
                                                                                gtagtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCa                                                                      >  2:1335571/1‑150 (MQ=255)
                                                                                 tagtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCag                                                                     <  2:1168427/150‑1 (MQ=255)
                                                                                  agtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATGTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCaga                                                                    >  2:593274/1‑150 (MQ=255)
                                                                                   gtaACGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCTGGAGGCCGGCCGTACATGCAGGCCGTTACTGGTGTTTATGCCCACCGCCtgag                                                                   >  2:90172/1‑150 (MQ=255)
                                                                                           aTCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGct                                                           <  1:1495569/150‑1 (MQ=255)
                                                                                            tCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGctt                                                          >  1:515939/1‑150 (MQ=255)
                                                                                             cTGGTTAATACTCTCTGGTGTGACGCATTTGCAGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCTTTACGGTTTTTTATGACCACCGCCAGACAACGGGctta                                                         >  1:1591330/1‑149 (MQ=255)
                                                                                              tGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGcttct                                                        <  1:405648/150‑1 (MQ=255)
                                                                                                gTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCttcttc                                                      >  2:1037716/1‑150 (MQ=255)
                                                                                                   aaTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATc                                                   >  2:75493/1‑150 (MQ=255)
                                                                                                    aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCCGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCCGCCGGGAGGCCGGACGTACATGCAGGCCGATACGGTTTTTTAAGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                  >  1:875278/1‑150 (MQ=255)
                                                                                                    aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCATGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                  <  1:1470193/150‑1 (MQ=255)
                                                                                                    aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                  <  1:1026255/150‑1 (MQ=255)
                                                                                                    aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                  <  1:545543/150‑1 (MQ=255)
                                                                                                    aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                  <  1:538650/150‑1 (MQ=255)
                                                                                                       ctctctGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTAc                                               <  2:6676/150‑1 (MQ=255)
                                                                                                         ctctGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCATCCGGGAGTCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTACATCGTACGc                                             >  1:658533/1‑150 (MQ=255)
                                                                                                             ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                         <  1:1332222/150‑1 (MQ=255)
                                                                                                             ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                         >  2:243480/1‑150 (MQ=255)
                                                                                                             ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAAAGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGATTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                         >  2:966585/1‑150 (MQ=255)
                                                                                                              gggtgACCCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGAc                                        <  1:286689/148‑1 (MQ=255)
                                                                                                               tgtgACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACa                                       <  2:22846/150‑1 (MQ=255)
                                                                                                                 tgACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtg                                     >  1:1650243/1‑150 (MQ=255)
                                                                                                                 tgACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtg                                     <  1:821471/150‑1 (MQ=255)
                                                                                                                   aCGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtgtg                                   <  1:1037716/150‑1 (MQ=255)
                                                                                                                         ttGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCagag                             >  1:576736/1‑150 (MQ=255)
                                                                                                                           gCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGAt                           >  1:1290413/1‑150 (MQ=255)
                                                                                                                              gTTTTTTTCCAGCAACATGTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTc                        <  1:593274/150‑1 (MQ=255)
                                                                                                                                ttttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                      <  1:939546/150‑1 (MQ=255)
                                                                                                                                ttttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGCCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                      <  2:253926/150‑1 (MQ=255)
                                                                                                                                 tttttCCAGCAACTTATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAgt                     <  1:1368447/150‑1 (MQ=255)
                                                                                                                                 tttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAgt                     <  1:1375175/150‑1 (MQ=255)
                                                                                                                                      ccAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCATGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgg                <  1:1126718/150‑1 (MQ=255)
                                                                                                                                      ccAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgg                >  1:785348/1‑150 (MQ=255)
                                                                                                                                       cAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgga               >  2:121285/1‑150 (MQ=255)
                                                                                                                                            ccttatCGACATTTAATGCCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACg          >  1:189394/4‑150 (MQ=255)
                                                                                                                                              atatCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTACAGTGTCGGAGGACGGt        >  2:777830/1‑150 (MQ=255)
                                                                                                                                               tatCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTa       <  2:1173367/150‑1 (MQ=255)
                                                                                                                                                  cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTCACGGTTTGTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTCCGCAGGACATGTGGCAGAGCTGTCAGTGTCGGAGGACGGTaacc    >  1:12887/1‑148 (MQ=255)
                                                                                                                                                   ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCTTACGCCGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc   >  1:152840/1‑150 (MQ=255)
                                                                                                                                                   ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc   >  1:526909/1‑150 (MQ=255)
                                                                                                                                                   ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc   <  1:75493/150‑1 (MQ=255)
                                                                                                                                                    gCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGACCGTGCTTCTTCAACGTACGCAGGTCATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAccc  >  2:1093201/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCTGACGGAAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCC  >  NC_000913/1248413‑1248710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: