Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,248,571 T→C 6.1% T182A (ACA→GCA)  dhaM ← putative dihydroxyacetone‑specific PTS enzymes: HPr, EI components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,248,5710TC 6.1% 285.3 / 6.9 99T182A (ACA→GCA) dhaMputative dihydroxyacetone‑specific PTS enzymes: HPr, EI components
Reads supporting (aligned to +/- strand):  ref base T (45/47);  new base C (2/4);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

AAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTC  >  NC_000913/1248423‑1248718
                                                                                                                                                    |                                                                                                                                                   
aaaGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTc                                                                                                                                                    <  1:437974/150‑1 (MQ=255)
   gCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGAt                                                                                                                                                 >  2:732464/1‑150 (MQ=255)
    cGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATa                                                                                                                                                <  2:588145/150‑1 (MQ=255)
     gATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATaa                                                                                                                                               >  1:1694804/1‑150 (MQ=255)
     gATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATat                                                                                                                                               <  1:760024/150‑2 (MQ=255)
      aTCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCAGCAACATATCGGCATTAAATGTCGATAAgg                                                                                                                                             <  1:13301/150‑1 (MQ=255)
       tCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATAATTCGCATCGTTTCGTTATAGCGAACTTGTAGTATAGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAgg                                                                                                                                             <  1:195983/150‑1 (MQ=255)
       tCAGTGCCTCATCAGAATCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGAGTAATTGTAGTAACTCAATCTGTTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTACCAGCAACATATCGACATTTAATGCCGATatgt                                                                                                                                             <  2:227050/150‑4 (MQ=255)
         aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtt                                                                                                                                           >  2:868878/1‑149 (MQ=255)
         aGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATatgttg                                                                                                                                           >  2:165193/1‑145 (MQ=255)
          gTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtat                                                                                                                                          >  2:1126718/1‑150 (MQ=255)
           tGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGtata                                                                                                                                         <  1:104219/150‑1 (MQ=255)
           tGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTTAATGCCGATatgttgct                                                                                                                                         <  2:936297/150‑8 (MQ=255)
              ctctTCAGCTTCTGGCGCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCACTGGTGTGACGCATTTGCCGTTTTTTTCCAGCATCATATCGGCATTAAATGTCGATAAGGTATAAAc                                                                                                                                      <  2:337293/150‑1 (MQ=255)
               tctTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCTAACTTGAAGTAACGCAATCTGGTTAATACTCTCTGGTGTTACGCATTTGCCGTTTTTTTCCAGCAACATCTCGGCATTAAATGTCGATAAGGTATAAAcc                                                                                                                                     <  1:337848/150‑1 (MQ=255)
                ctTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTACAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCa                                                                                                                                    >  2:1401461/1‑150 (MQ=255)
                 ttCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCac                                                                                                                                   >  2:1275236/1‑149 (MQ=255)
                   cAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGcc                                                                                                                                 >  2:821471/1‑150 (MQ=255)
                     gCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCgg                                                                                                                               <  1:623804/150‑1 (MQ=255)
                             cccTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCgg                                                                                                                       >  1:1120346/1‑150 (MQ=255)
                              ccTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGa                                                                                                                      <  1:732464/150‑1 (MQ=255)
                               cTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGAc                                                                                                                     <  2:1184119/150‑1 (MQ=255)
                               cTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGAc                                                                                                                     <  2:643552/150‑1 (MQ=255)
                                tttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACg                                                                                                                    <  1:55425/150‑1 (MQ=255)
                                 ttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGt                                                                                                                   >  2:1368447/1‑150 (MQ=255)
                                 ttCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGt                                                                                                                   >  2:1375175/1‑150 (MQ=255)
                                   cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGTCATTAAATGTCGATAAGGTTTAAAACATCCGGGAGGCCGGACGTaa                                                                                                                 >  1:1216326/1‑149 (MQ=255)
                                   cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGTCCGGACGTAc                                                                                                                 >  1:213357/1‑150 (MQ=255)
                                   cGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAAAACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTAAAACCCAGCCGGGAGGCCGGACGTAc                                                                                                                 >  1:156536/1‑150 (MQ=255)
                                       aTCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGc                                                                                                             >  1:428138/1‑150 (MQ=255)
                                           ggCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGc                                                                                                         <  1:1654223/150‑1 (MQ=255)
                                             cgcAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCg                                                                                                       >  2:1495569/1‑150 (MQ=255)
                                              gtaGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATAGTCATTAAATGTAGATAAGGTATAAACCAGCCGGGAGGCAGGACGTACATGCAGGCCGt                                                                                                      >  1:727184/3‑150 (MQ=255)
                                               cAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGtt                                                                                                     <  1:1265589/150‑1 (MQ=255)
                                               cAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGtt                                                                                                     >  2:210861/1‑150 (MQ=255)
                                                       cGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGtttt                                                                                             <  1:346129/150‑1 (MQ=255)
                                                        gTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTGACGGttttt                                                                                            >  2:1222824/1‑150 (MQ=255)
                                                         ttATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGtttttt                                                                                           <  2:1200753/150‑1 (MQ=255)
                                                          tataGCGAACTTGTAGTAACGCAATCTGGGTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTACATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTa                                                                                          <  1:1087139/150‑1 (MQ=255)
                                                              gCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATTTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGac                                                                                      >  2:1516895/1‑150 (MQ=255)
                                                              gCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGac                                                                                      >  2:1466378/1‑150 (MQ=255)
                                                               cGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGacc                                                                                     <  1:1691306/150‑1 (MQ=255)
                                                               cGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGacc                                                                                     <  2:1120346/150‑1 (MQ=255)
                                                                     tGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCAGTTACGGTTTTTTATGACCACCGcc                                                                               >  1:198882/1‑150 (MQ=255)
                                                                      gtagtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCa                                                                              >  2:1335571/1‑150 (MQ=255)
                                                                       tagtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCag                                                                             <  2:1168427/150‑1 (MQ=255)
                                                                        agtaACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATGTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCaga                                                                            >  2:593274/1‑150 (MQ=255)
                                                                         gtaACGCAATCTGGTTAATACTCTCTGGTGTGACTCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCTGGAGGCCGGCCGTACATGCAGGCCGTTACTGGTGTTTATGCCCACCGCCtgag                                                                           >  2:90172/1‑150 (MQ=255)
                                                                                 aTCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGct                                                                   <  1:1495569/150‑1 (MQ=255)
                                                                                  tCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGctt                                                                  >  1:515939/1‑150 (MQ=255)
                                                                                   cTGGTTAATACTCTCTGGTGTGACGCATTTGCAGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCTTTACGGTTTTTTATGACCACCGCCAGACAACGGGctta                                                                 >  1:1591330/1‑149 (MQ=255)
                                                                                    tGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGcttct                                                                <  1:405648/150‑1 (MQ=255)
                                                                                      gTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCttcttc                                                              >  2:1037716/1‑150 (MQ=255)
                                                                                         aaTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATc                                                           >  2:75493/1‑150 (MQ=255)
                                                                                          aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCCGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCCGCCGGGAGGCCGGACGTACATGCAGGCCGATACGGTTTTTTAAGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                          >  1:875278/1‑150 (MQ=255)
                                                                                          aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCATGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                          <  1:1470193/150‑1 (MQ=255)
                                                                                          aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                          <  1:545543/150‑1 (MQ=255)
                                                                                          aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                          <  1:1026255/150‑1 (MQ=255)
                                                                                          aTACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCg                                                          <  1:538650/150‑1 (MQ=255)
                                                                                             ctctctGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTAc                                                       <  2:6676/150‑1 (MQ=255)
                                                                                               ctctGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCATCCGGGAGTCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTACATCGTACGc                                                     >  1:658533/1‑150 (MQ=255)
                                                                                                   ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                                 >  2:243480/1‑150 (MQ=255)
                                                                                                   ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                                 <  1:1332222/150‑1 (MQ=255)
                                                                                                   ggTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAAAGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGATTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGa                                                 >  2:966585/1‑150 (MQ=255)
                                                                                                    gggtgACCCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGAc                                                <  1:286689/148‑1 (MQ=255)
                                                                                                     tgtgACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACa                                               <  2:22846/150‑1 (MQ=255)
                                                                                                       tgACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtg                                             >  1:1650243/1‑150 (MQ=255)
                                                                                                       tgACGCATTTGCCGTTTTTTTCCAGCAACATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtg                                             <  1:821471/150‑1 (MQ=255)
                                                                                                         aCGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAtgtg                                           <  1:1037716/150‑1 (MQ=255)
                                                                                                               ttGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCagag                                     >  1:576736/1‑150 (MQ=255)
                                                                                                                 gCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGAt                                   >  1:1290413/1‑150 (MQ=255)
                                                                                                                    gTTTTTTTCCAGCAACATGTCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTc                                <  1:593274/150‑1 (MQ=255)
                                                                                                                      ttttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                              <  1:939546/150‑1 (MQ=255)
                                                                                                                      ttttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGCCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                              <  2:253926/150‑1 (MQ=255)
                                                                                                                       tttttCCAGCAACTTATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAgt                             <  1:1368447/150‑1 (MQ=255)
                                                                                                                       tttttCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAgt                             <  1:1375175/150‑1 (MQ=255)
                                                                                                                            ccAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCATGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgg                        <  1:1126718/150‑1 (MQ=255)
                                                                                                                            ccAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgg                        >  1:785348/1‑150 (MQ=255)
                                                                                                                             cAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCgga                       >  2:121285/1‑150 (MQ=255)
                                                                                                                                  ccttatCGACATTTAATGCCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACg                  >  1:189394/4‑150 (MQ=255)
                                                                                                                                    atatCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTACAGTGTCGGAGGACGGt                >  2:777830/1‑150 (MQ=255)
                                                                                                                                     tatCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTa               <  2:1173367/150‑1 (MQ=255)
                                                                                                                                        cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTCACGGTTTGTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTCCGCAGGACATGTGGCAGAGCTGTCAGTGTCGGAGGACGGTaacc            >  1:12887/1‑148 (MQ=255)
                                                                                                                                         ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCTTACGCCGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc           >  1:152840/1‑150 (MQ=255)
                                                                                                                                         ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc           <  1:75493/150‑1 (MQ=255)
                                                                                                                                         ggCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAcc           >  1:526909/1‑150 (MQ=255)
                                                                                                                                          gCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGACCGTGCTTCTTCAACGTACGCAGGTCATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAAccc          >  2:1093201/1‑150 (MQ=255)
                                                                                                                                           cATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                              >  2:1408489/1‑129 (MQ=255)
                                                                                                                                           cATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAg                              <  1:1408489/129‑1 (MQ=255)
                                                                                                                                           cATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCa         >  1:1562538/1‑150 (MQ=255)
                                                                                                                                            aatAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTTAGTGTCGCAGGACGGTAAACCCAg        <  2:630179/148‑1 (MQ=255)
                                                                                                                                            aTTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCGTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAg        <  1:1275236/150‑1 (MQ=255)
                                                                                                                                            aTTAAATGGCGATAAGGTATAAACCAGCCGGGAGGCCGTACGTACATGCCGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAg        <  2:931301/150‑1 (MQ=255)
                                                                                                                                             ttAATTGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGTCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATTTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAgt       <  1:353837/150‑1 (MQ=255)
                                                                                                                                             ttAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAgt       <  2:1618565/150‑1 (MQ=255)
                                                                                                                                               aaaTGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCCCCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACAAGTGTCAGAGCTTTCAGTGTCGGAGGACGTTAACCCAggtga    >  2:495969/1‑149 (MQ=255)
                                                                                                                                               aaaTGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAgttg     <  1:1027804/150‑1 (MQ=255)
                                                                                                                                                 aTGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCCGGCCGTTACGGTTTTTTATGCCCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAgttgtt   <  2:694115/150‑1 (MQ=255)
                                                                                                                                                  tGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTc  <  2:1694804/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAAGCGATCAGTGCCTCTTCAGCTTCTGGCCCTTTCGCAATCAGGCGCAGCGTATCGTTATAGCGAACTTGTAGTAACGCAATCTGGTTAATACTCTCTGGTGTGACGCATTTGCCGTTTTTTTCCAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTC  >  NC_000913/1248423‑1248718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: