Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,330,399 A→T 6.9% intergenic (+18/+18) sohB → / ← yciN inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,330,3990AT 6.9% 329.0 / 10.3 115intergenic (+18/+18)sohB/yciNinner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
Reads supporting (aligned to +/- strand):  ref base A (49/58);  new base T (3/5);  total (52/63)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 117 total aligned reads displayed.

GGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACG  >  NC_000913/1330250‑1330546
                                                                                                                                                      |                                                                                                                                                   
ggCCGTGAAGTGGTCAATGTTCGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTa                                                                                                                                                     <  1:1388860/150‑1 (MQ=255)
  ccGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAg                                                                                                                                                   >  1:385085/1‑150 (MQ=255)
  ccGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAg                                                                                                                                                   <  1:1240530/150‑1 (MQ=255)
    gTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGAc                                                                                                                                                 <  1:620061/150‑1 (MQ=255)
        aGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcg                                                                                                                                             <  2:1599940/150‑1 (MQ=255)
        aGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcg                                                                                                                                             <  1:737949/150‑1 (MQ=255)
         gTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgc                                                                                                                                            <  2:1248345/150‑1 (MQ=255)
         gTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgc                                                                                                                                            >  2:620761/1‑150 (MQ=255)
         gTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGAATCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTTATGTAAAAGACAAACGCGAGGCTAAGACCTcgc                                                                                                                                            <  2:1112603/150‑1 (MQ=255)
          tGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgcg                                                                                                                                           <  1:715555/150‑1 (MQ=255)
          gggTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGTTCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTcgcg                                                                                                                                           <  2:1048232/149‑1 (MQ=255)
           ggTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGt                                                                                                                                          >  1:673029/1‑150 (MQ=255)
           ggTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGt                                                                                                                                          >  1:856459/1‑150 (MQ=255)
           ggTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGt                                                                                                                                          >  1:599822/1‑150 (MQ=255)
            ggcAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtt                                                                                                                                         <  1:955923/148‑1 (MQ=255)
            gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtt                                                                                                                                         <  1:1287702/150‑1 (MQ=255)
              cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGtttg                                                                                                                                       <  1:904962/150‑2 (MQ=255)
              cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGtttt                                                                                                                                       >  2:959694/1‑150 (MQ=255)
                       cTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATc                                                                                                                              <  2:359134/150‑1 (MQ=255)
                       cTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATc                                                                                                                              >  1:779857/1‑150 (MQ=255)
                       cTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGGCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATc                                                                                                                              <  1:837658/150‑1 (MQ=255)
                        tataTGCAGCGTAAACGACTCATTGACCGTTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTAGGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGCAAAACGCGAGGTCTTAGCCTCGCGTTTGTCttttacat                                                                                                                             <  1:469387/150‑6 (MQ=37)
                        tataTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGTGGCTAAGACCTCGCGTTTTGCTTTAATCa                                                                                                                             >  1:1003320/1‑150 (MQ=255)
                        tataTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGTGGCTAAGACCTCGCGTTTTGCTTTAATCa                                                                                                                             >  1:996833/1‑150 (MQ=255)
                         atatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAGCCTCGCGTTTGTCttttacatc                                                                                                                            <  1:1406626/150‑7 (MQ=38)
                           atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAAcc                                                                                                                          <  1:1242854/150‑1 (MQ=255)
                                 cGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAgatga                                                                                                                    >  2:381401/1‑150 (MQ=255)
                                   tAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGata                                                                                                                  <  2:833843/150‑1 (MQ=255)
                                    aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGatat                                                                                                                 <  1:1108553/150‑1 (MQ=255)
                                    aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGatat                                                                                                                 >  2:55614/1‑150 (MQ=255)
                                      aCGACTCATTGACCGAATCACCGGCAGCGCGGCAGCGAGCGCCGATCGAATGTTGCTACGCTGGGGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGCGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAttt                                                                                                               <  2:307565/150‑1 (MQ=255)
                                         aCTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc                                                                                                            >  2:97308/1‑150 (MQ=255)
                                              ttGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa                                                                                                       <  2:977407/150‑1 (MQ=255)
                                                gACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGc                                                                                                     >  1:1421890/1‑150 (MQ=255)
                                                 cccGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGca                                                                                                    <  1:1559250/149‑1 (MQ=255)
                                                  ccGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTATGACCTCTCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac                                                                                                   >  2:1072428/1‑150 (MQ=255)
                                                   cGATTCACCTGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCATATGATATTTTTCTGAAAGcaca                                                                                                  >  2:599270/1‑150 (MQ=255)
                                                        cACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGc                                                                                             >  2:1239537/1‑150 (MQ=255)
                                                           cGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAg                                                                                          >  2:155233/1‑150 (MQ=255)
                                                            ggCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAgg                                                                                         >  1:1285795/1‑150 (MQ=255)
                                                              cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtg                                                                                       <  1:1601941/150‑1 (MQ=255)
                                                              cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgtg                                                                                       >  2:1100195/1‑150 (MQ=255)
                                                                gcgcGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGtt                                                                                     <  2:599822/150‑1 (MQ=255)
                                                                  gcgGCAGTGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTg                                                                                   <  2:1552623/150‑1 (MQ=255)
                                                                  gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTg                                                                                   <  2:856459/150‑1 (MQ=255)
                                                                   cgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAGCCATTGATGTAAACGACAAACGCGAGGCTAAGACCTCGCGTGTTGCTTTAATCAACCAGATGATAGTTTTCTGAAAGCACAAGGGCCAGGTGTTTgca                                                                                 >  1:320283/1‑148 (MQ=255)
                                                                     gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                <  2:779857/150‑1 (MQ=255)
                                                                     gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                <  2:1639612/150‑1 (MQ=255)
                                                                     gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                <  1:417634/150‑1 (MQ=255)
                                                                      cAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACa                                                                               >  1:1299595/1‑150 (MQ=255)
                                                                         agagCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACatat                                                                            >  2:1100059/1‑150 (MQ=255)
                                                                         agagCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACatat                                                                            <  1:1430605/150‑1 (MQ=255)
                                                                              gcCGATCGATCGCGGCTACGCTGGTGGCAGCGGGGGCCAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAac                                                                       <  2:320210/150‑1 (MQ=255)
                                                                               ccGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAaca                                                                      >  1:42327/1‑150 (MQ=255)
                                                                                  atcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACcg                                                                   >  1:225281/1‑150 (MQ=255)
                                                                                          ttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCtt                                                           <  1:1119767/150‑1 (MQ=255)
                                                                                           tgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCttt                                                          >  2:1099077/1‑150 (MQ=255)
                                                                                            gCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCtttt                                                         >  1:1064930/1‑150 (MQ=255)
                                                                                                  cTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGt                                                   <  1:1024025/150‑1 (MQ=255)
                                                                                                    ggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTg                                                 <  2:1230112/150‑1 (MQ=255)
                                                                                                      tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                               <  2:658410/150‑1 (MQ=255)
                                                                                                      tggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                               <  1:1732644/150‑1 (MQ=255)
                                                                                                      tgccAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACata                                                                             >  1:773862/4‑120 (MQ=255)
                                                                                                      tgccAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACata                                                                             <  2:773862/117‑1 (MQ=255)
                                                                                                      gggaaGCGGGGTCAAAAGCAATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCATGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGc                                               <  2:838539/146‑1 (MQ=255)
                                                                                                       ggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCa                                              >  2:395941/1‑150 (MQ=255)
                                                                                                         cAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCCCATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAAt                                            >  2:327482/1‑150 (MQ=255)
                                                                                                           gCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATcc                                          <  1:644838/150‑1 (MQ=255)
                                                                                                            cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCt                                         <  1:959694/150‑1 (MQ=255)
                                                                                                            cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCt                                         <  1:1296659/150‑1 (MQ=255)
                                                                                                            cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCt                                         <  1:1497702/150‑1 (MQ=255)
                                                                                                                gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                     >  2:1141953/1‑150 (MQ=255)
                                                                                                                 tCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                    <  2:360364/150‑1 (MQ=255)
                                                                                                                  cAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTATTGCTTTAATCAACCAGATGATAATATTCTGAAAGCACATGGGACAGCTGTTTGAAAATAATAAACACCGAGGTTCTTTAGGCTGTTGGCAATCgttgttaa                                   >  1:719407/1‑148 (MQ=255)
                                                                                                                    aaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc                                 <  1:713603/150‑1 (MQ=255)
                                                                                                                    aaaGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATc                                 <  1:837982/150‑1 (MQ=255)
                                                                                                                       gCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaa                              >  1:829817/1‑150 (MQ=255)
                                                                                                                       gCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaa                              >  1:739546/1‑150 (MQ=255)
                                                                                                                         cATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAAACAACCAGATGATATTTTTCTGAAAGCACATGTTACAGGTGATTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGTCAATCGGTGTTCATATaaaa                            >  2:93717/1‑150 (MQ=255)
                                                                                                                           ttGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAag                          <  1:1513750/150‑1 (MQ=255)
                                                                                                                            tGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagt                         <  1:685628/150‑1 (MQ=255)
                                                                                                                             gATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagta                        >  2:705373/1‑150 (MQ=255)
                                                                                                                              aTGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                       <  1:97308/150‑1 (MQ=255)
                                                                                                                              aTGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtag                       >  1:1596661/1‑150 (MQ=255)
                                                                                                                                gTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTc                     >  1:391600/1‑150 (MQ=255)
                                                                                                                                gTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTCTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAagtagta                     >  2:1307745/1‑149 (MQ=255)
                                                                                                                                   aaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc                  >  1:185289/1‑150 (MQ=255)
                                                                                                                                   aaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc                  <  1:1601532/150‑1 (MQ=255)
                                                                                                                                   aaaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGcc                  <  1:399435/150‑1 (MQ=255)
                                                                                                                                    aaGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAGCCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACCCCGCGGTGCTTTTGGCTGTTGGCAATCCTAGTTCATCTAAAAAGTAGTCGCCg                 >  1:330377/1‑150 (MQ=255)
                                                                                                                                      gcaaaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt               >  2:594128/4‑150 (MQ=255)
                                                                                                                                      gcaaaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt               >  2:1325395/4‑150 (MQ=255)
                                                                                                                                      gACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGt               <  1:155233/150‑1 (MQ=255)
                                                                                                                                       aCAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTa              <  1:1202290/150‑1 (MQ=255)
                                                                                                                                         aaaCGCGAGGTCTTAGCCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaaa            >  2:990491/1‑150 (MQ=255)
                                                                                                                                             gcgAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAcc        <  2:517436/150‑1 (MQ=255)
                                                                                                                                             gcgAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATAcc        <  1:543719/150‑1 (MQ=255)
                                                                                                                                                 ggCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTATAAAGTAGTCGCCGGTAAATACCAGCa    >  2:1339227/1‑150 (MQ=255)
                                                                                                                                                 ggCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa    >  1:1410766/1‑150 (MQ=255)
                                                                                                                                                    cTAAGACCTCTCGTTTTGCTTTTATCAACCTGATGATAATTTTGTTAAAGCTCATTGGTCAGGTGTTTGTACATTTTAAACTCCGCGGTGCTTTTGGCTGTTGGCATTCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACg  <  1:25365/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAACACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACG  >  NC_000913/1330250‑1330546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: