Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,354,224 C→T 5.4% V99M (GTG→ATG) ‡ sapC ← antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,354,2240CT 5.4% 291.5 / 2.3 91V99M (GTG→ATG) ‡sapCantimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (47/39);  new base T (2/3);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGG  >  NC_000913/1354076‑1354368
                                                                                                                                                    |                                                                                                                                                
aTTGCCAACAGCAGCGAAGGGATCGCCAGTGAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACg                                                                                                                                                 >  2:1351199/1‑150 (MQ=255)
 tagCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACgg                                                                                                                                                >  1:342462/3‑150 (MQ=255)
   gCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGtt                                                                                                                                              <  1:191827/150‑1 (MQ=255)
   gCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGtt                                                                                                                                              >  2:780623/1‑150 (MQ=255)
    ccAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTg                                                                                                                                             >  1:1292852/1‑150 (MQ=255)
     cAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTgg                                                                                                                                            >  2:909532/1‑150 (MQ=255)
     cAACAGCAGCGAAGGGATCGCCAGTAAGGTATACAGAAAATGGTTAAGCACCGCTGACCGGAGGCCGTGTGTCGCCCCGGCCAAGGAGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCCCCCACggatcg                                                                                                                                            >  1:616565/1‑146 (MQ=255)
      aaCAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgc                                                                                                                                           <  1:1705674/150‑1 (MQ=255)
           cagcGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCAACCGTGGGTGGCGCatt                                                                                                                                      <  1:331540/150‑3 (MQ=255)
               gAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCTCCACCCACGGTTGGCGCAGCTCCg                                                                                                                                  >  1:671609/1‑150 (MQ=255)
               gAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCAACCGTGGGTGGCGCatttgtg                                                                                                                                  <  1:443655/150‑7 (MQ=255)
               gAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTACCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCAACCGTGGGTGGCGCatttgtg                                                                                                                                  <  1:1653835/150‑7 (MQ=255)
                aaGGGAGCTCCAGTAAGGGATCCAGATTATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGTCCACAGATCGTCGCGGCTAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGc                                                                                                                                 <  2:32824/150‑1 (MQ=255)
                  gggATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTc                                                                                                                               <  2:673456/150‑1 (MQ=255)
                   ggATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGCTGGTGCCCAGTACCTGGCCACAGCTCGTAGCGGCCAGCGTCACCACCAATGCGCCACCGACGGTTGGCGCAGCTCCGcgcc                                                                                                                              >  2:1055885/1‑147 (MQ=255)
                       cGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGCTCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTaa                                                                                                                          >  1:70645/1‑150 (MQ=255)
                       cGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTaa                                                                                                                          >  1:12374/1‑150 (MQ=255)
                        gCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTaaa                                                                                                                         <  1:377522/150‑1 (MQ=255)
                         ccAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAAc                                                                                                                        >  1:510184/1‑150 (MQ=255)
                            gTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGc                                                                                                                     <  1:1461228/150‑1 (MQ=255)
                             tAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCt                                                                                                                    >  1:1627706/1‑150 (MQ=255)
                               aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTa                                                                                                                  <  2:407024/150‑1 (MQ=255)
                               aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTa                                                                                                                  <  1:960177/150‑1 (MQ=255)
                               aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTa                                                                                                                  <  2:607167/150‑1 (MQ=255)
                                ggTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTaa                                                                                                                 >  2:1565162/1‑150 (MQ=255)
                                 gTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTaaa                                                                                                                >  2:913651/1‑150 (MQ=255)
                                 gTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGACGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACGCCAAATGCGCCCCCCACGGTTGGCGCAGCGCCGCTCAGTAGACGGCTTaaa                                                                                                                >  1:77436/1‑150 (MQ=255)
                                     ccAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACAt                                                                                                            <  2:342462/150‑1 (MQ=255)
                                       aGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATcg                                                                                                          <  2:128730/150‑1 (MQ=255)
                                             tGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACAGCGCGTCCg                                                                                                    >  1:1103520/1‑150 (MQ=255)
                                              ggTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCgg                                                                                                   >  2:343694/1‑149 (MQ=255)
                                               gTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg                                                                                                  >  1:1129358/1‑150 (MQ=255)
                                               gTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATAGCGTCCGAg                                                                                                  >  2:1589651/1‑150 (MQ=255)
                                               gTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCGTAAAACATCGCGTCCGAg                                                                                                  >  2:1149539/1‑150 (MQ=255)
                                                ttAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAgg                                                                                                 <  2:117658/150‑1 (MQ=255)
                                                   aGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcg                                                                                              <  2:1619725/150‑1 (MQ=255)
                                                   aGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcg                                                                                              >  2:925482/1‑150 (MQ=255)
                                                   aGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcg                                                                                              >  1:313687/1‑150 (MQ=255)
                                                    gCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCCCCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgt                                                                                             >  1:403793/1‑150 (MQ=255)
                                                    gCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgg                                                                                             >  1:1035374/1‑149 (MQ=255)
                                                     ctccGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgtc                                                                                            <  2:1265313/148‑1 (MQ=255)
                                                     cACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgtc                                                                                            <  2:12726/150‑1 (MQ=255)
                                                     cACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgtc                                                                                            <  2:295027/150‑1 (MQ=255)
                                                     cACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgtc                                                                                            <  2:1292852/150‑1 (MQ=255)
                                                      aCCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCa                                                                                           >  1:766060/1‑150 (MQ=255)
                                                      aCCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCa                                                                                           >  1:997254/1‑150 (MQ=255)
                                                                ggAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGga                                                                                 >  1:1033996/1‑150 (MQ=255)
                                                                 gAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCCCGGTGGGCGCAGCTCCGCTCAGGAAACGGCTTAAAACCTCGCGTCCGAGGTCGTCAGTCCCCAGgaa                                                                                >  1:259561/1‑150 (MQ=255)
                                                                   ggCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGGGCAGTAAACGGCGTAAATCAGCGCGGCCGAGGTCGTCGGGCCCCAGgtagt                                                                              >  1:660033/1‑149 (MQ=255)
                                                                       gtgtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGGCGTCAGTCCCCAGGaagaaagc                                                                          >  1:170948/1‑149 (MQ=255)
                                                                         gtgtCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAgaaagaaa                                                                        <  1:866631/150‑1 (MQ=255)
                                                                          tgtCGCCCCGGCGAATGTGCCCAGTACCAGGCCACAGATCGTCGCGTCAAGCGTCACCACAATTGCGCCACCCTCGGTTGGCGAAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAAc                                                                       <  2:1254949/150‑1 (MQ=255)
                                                                             cGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTc                                                                    <  1:753746/150‑1 (MQ=255)
                                                                              gCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCCGTCCCCAGGAAGAAAGAAACTTCg                                                                   >  2:1060326/1‑150 (MQ=255)
                                                                              gCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCg                                                                   >  2:609900/1‑150 (MQ=255)
                                                                                  cGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATTCGCCACCCACGGGTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCCGGAAGAAAGAAACTTCGCCAt                                                               <  1:620349/150‑1 (MQ=255)
                                                                                  cGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCAt                                                               <  2:458137/150‑1 (MQ=255)
                                                                                          gTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGAc                                                       >  1:69406/1‑150 (MQ=255)
                                                                                           tGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGAcc                                                      <  2:766060/150‑1 (MQ=255)
                                                                                           tGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGAcc                                                      <  2:1480615/150‑1 (MQ=255)
                                                                                           tGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGAcc                                                      <  2:997254/150‑1 (MQ=255)
                                                                                                 gTACCAGGCCACAGATCGTCGCGGCAAGCGTCCCCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGCcg                                                >  2:1080855/1‑150 (MQ=255)
                                                                                                 gTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcg                                                <  1:500843/150‑1 (MQ=255)
                                                                                                   aCCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggc                                              <  2:313687/150‑1 (MQ=255)
                                                                                                     cAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAACCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGCCCATGAcggcgg                                            >  1:1577270/1‑150 (MQ=255)
                                                                                                         ccACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaat                                        >  2:1657517/1‑150 (MQ=255)
                                                                                                            caGATCGTCGCGGCATGCGTCACCACAACTGCGCGACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaataat                                     <  2:1586065/150‑1 (MQ=255)
                                                                                                            caGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaataat                                     >  1:812055/1‑150 (MQ=255)
                                                                                                              gATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTg                                   <  1:909532/150‑1 (MQ=255)
                                                                                                                   tcgCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAAc                              <  1:815157/150‑1 (MQ=255)
                                                                                                                           aGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAACCTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAAt                      >  2:1272794/1‑150 (MQ=255)
                                                                                                                            gCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAAtt                     <  2:350493/150‑1 (MQ=255)
                                                                                                                              gTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGc                   >  1:704100/1‑150 (MQ=255)
                                                                                                                               tCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGTCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAATAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGAAATAATTGATAAGCGAGAAATTGCt                  <  1:134029/150‑1 (MQ=255)
                                                                                                                                caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTg                 >  2:1171246/1‑150 (MQ=255)
                                                                                                                                 accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCTATATTTGATAACCGAGAAATTGCTgg                >  2:1172507/1‑150 (MQ=255)
                                                                                                                                 accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACATCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                <  1:1512241/150‑1 (MQ=255)
                                                                                                                                 accacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCATCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                <  2:1008998/150‑1 (MQ=255)
                                                                                                                                  ccacAAACGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCATCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGt               <  1:1401037/150‑1 (MQ=255)
                                                                                                                                   cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc              <  2:196151/150‑1 (MQ=255)
                                                                                                                                   cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc              >  2:201173/1‑150 (MQ=255)
                                                                                                                                   cacaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTAAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGATAGCAACTTCGCCTTAGCGCGCCCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTc              >  1:831623/1‑150 (MQ=255)
                                                                                                                                      agctGCGCCAACCGTGGGTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCCGGAAGAACGAAACTTCGCCATCGCGCGACCATGCCGGCGGCAATAATTGCTAACCGAGAAATTGCTGGTCGAt           >  1:842584/4‑150 (MQ=255)
                                                                                                                                      aaaTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGAt           >  1:1013036/1‑150 (MQ=255)
                                                                                                                                       aaTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACCTCGCGTCCGAGGTCGTCAGTCACCAGGAAGAAAGAAACTTCGCCATAGCGCGCCCATGACGGCGGCATTAATTGATAACCCAGAAATTGCTGGTCGCTg          >  1:1339917/1‑150 (MQ=255)
                                                                                                                                       aaTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATg          <  2:70645/150‑1 (MQ=255)
                                                                                                                                       aaTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATg          <  2:12374/150‑1 (MQ=255)
                                                                                                                                        aTGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGc         >  1:185521/1‑150 (MQ=255)
                                                                                                                                         tGCGCCACCCACGTTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCTCGTCCGAGTTCGTCAGTCCCCATGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGcc        <  1:1027440/150‑1 (MQ=255)
                                                                                                                                         tGCGCCAACCGTGGGTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGcc        >  1:908090/1‑150 (MQ=255)
                                                                                                                                           cgcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGt      >  1:1614741/1‑150 (MQ=255)
                                                                                                                                             ccacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACCTCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTaa    >  1:109990/1‑150 (MQ=255)
                                                                                                                                               ccccacGGTGTGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg  <  1:1351199/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGG  >  NC_000913/1354076‑1354368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: