Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,357,224 G→A 6.3% intergenic (‑114/+199) sapA ← / ← ymjA antimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,357,2240GA 6.3% 350.2 / 7.9 110intergenic (‑114/+199)sapA/ymjAantimicrobial peptide transport ABC transporter periplasmic binding protein/DUF2543 family protein
Reads supporting (aligned to +/- strand):  ref base G (57/46);  new base A (3/4);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 114 total aligned reads displayed.

CAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGT  >  NC_000913/1357077‑1357372
                                                                                                                                                   |                                                                                                                                                    
cAATCACCAAATGAGACGATAAAACCTGGCGCATAATCTTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGGCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTg                                                                                                                                                    <  2:101419/150‑1 (MQ=255)
   tCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACg                                                                                                                                                 >  2:1420787/1‑150 (MQ=255)
    cACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGt                                                                                                                                                >  1:1696279/1‑150 (MQ=255)
      ccAAAATAGAGTATAATCCATGGCGCTTAATATTAAGGTATTTTCTGTAAAGCGGCTATCTTACTAATAGTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCGGTGTGGTTGGTCGTTGAACAGGTGacgacg                                                                                                                                              <  2:314043/150‑1 (MQ=255)
      ccAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCg                                                                                                                                              >  1:396105/1‑150 (MQ=255)
         aaaGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAAAATTTAATGCCATTTGCCATTACCGTTAGTGTTCAGGGGTCGTAATCAGGGCCGTGTGGTTGGTCGTAGAACAGGTGACGTCGACa                                                                                                                                           >  2:221810/1‑150 (MQ=255)
          aaccgaCGATAATACCTGGCGCATACTATTCAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATAACCGTTTGTGGGCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCAt                                                                                                                                          <  1:84860/146‑1 (MQ=255)
              gaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGt                                                                                                                                      >  1:719692/1‑150 (MQ=255)
              gaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGt                                                                                                                                      >  1:389085/1‑150 (MQ=255)
              gaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGACCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGt                                                                                                                                      >  1:1220988/1‑150 (MQ=255)
                 gATAATACCTGGCTCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                   <  2:614896/150‑1 (MQ=255)
                 gATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTCATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                   <  2:149633/150‑1 (MQ=255)
                 gATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                   <  2:1353768/150‑1 (MQ=255)
                 gATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCa                                                                                                                                   <  2:1372469/150‑1 (MQ=255)
                 gATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGATGGCGACGTCACCTGTTCa                                                                                                                                   <  2:185057/150‑1 (MQ=255)
                   taataCCTGGCGCATAAAATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGATGGCGACGTCACCTGTTCAAc                                                                                                                                 <  1:308303/150‑1 (MQ=255)
                   aaataCCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGATGGCGACGTCACCTGTTCAAc                                                                                                                                 <  1:1468602/149‑1 (MQ=255)
                        ccTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCg                                                                                                                            <  1:600178/150‑1 (MQ=255)
                              gcATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTTTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCg                                                                                                                      <  1:1159451/150‑1 (MQ=255)
                                 taataTTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGa                                                                                                                   >  1:1353454/1‑150 (MQ=255)
                                  aataTTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGAt                                                                                                                  >  2:1631977/1‑150 (MQ=255)
                                      ttAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGATGACGTCGCCATCTGTTCAACATTCGTCCCCGTGATTtct                                                                                                              >  2:1435503/1‑150 (MQ=255)
                                        aaGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTctct                                                                                                            >  2:1438763/1‑150 (MQ=255)
                                         aGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTcta                                                                                                           <  2:1633540/150‑1 (MQ=255)
                                          gggATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGGGTGGTGGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctat                                                                                                          <  2:1290298/150‑1 (MQ=255)
                                           ggATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctata                                                                                                         <  1:1072301/150‑1 (MQ=255)
                                             aTTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCTATCTGTTCAACATTCGTACCCGTGATTTCTctataaa                                                                                                       >  2:1295202/1‑149 (MQ=255)
                                             aTTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataac                                                                                                       >  1:1318069/1‑150 (MQ=255)
                                             aTTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataac                                                                                                       >  2:90790/1‑150 (MQ=255)
                                              ttttATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTCCCGTTTGTGTTCAGGGGGCGTAATGAGGGCCGTGGGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataact                                                                                                      <  2:1189650/150‑1 (MQ=255)
                                              ttttATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataact                                                                                                      >  2:580618/1‑150 (MQ=255)
                                                ttATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataactat                                                                                                    <  2:518878/150‑1 (MQ=255)
                                                 tATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataactata                                                                                                   <  1:970511/150‑1 (MQ=255)
                                                 tATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTctataactata                                                                                                   >  1:1214584/1‑150 (MQ=255)
                                                    gTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTataactataact                                                                                                <  2:187465/150‑1 (MQ=255)
                                                      aaaGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTca                                                                                              >  2:1218614/1‑150 (MQ=255)
                                                      aaaGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTca                                                                                              >  2:318071/1‑150 (MQ=255)
                                                        agagGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTcaca                                                                                            <  1:1602991/150‑1 (MQ=255)
                                                          agGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGa                                                                                          <  1:800236/150‑1 (MQ=255)
                                                           ggCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGaa                                                                                         >  2:307434/1‑150 (MQ=255)
                                                              tATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGaacaa                                                                                      >  1:1126601/1‑150 (MQ=255)
                                                              tATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACAATCGTACCCGTGATTTCTCTATAACTATAACTAACAGaacaa                                                                                      >  2:100786/1‑150 (MQ=255)
                                                               aTCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGTTCTTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGaacaac                                                                                     <  2:483194/150‑1 (MQ=255)
                                                                   tACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAg                                                                                 >  1:220484/1‑150 (MQ=255)
                                                                    aCTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGc                                                                                <  1:478699/150‑1 (MQ=255)
                                                                     cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCg                                                                               >  1:1725683/1‑150 (MQ=255)
                                                                     cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCg                                                                               >  2:845482/1‑150 (MQ=255)
                                                                     cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGAGTCGTAAAGAGGGCCGTGTGGTTGGTCGTTGAACAGGCGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCg                                                                               >  2:752458/1‑150 (MQ=255)
                                                                     cTAATATTTAATGACATTTGCCATTACAGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAgag                                                                               >  2:645588/1‑148 (MQ=255)
                                                                      tAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCga                                                                              >  1:1575675/1‑150 (MQ=255)
                                                                      tAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCga                                                                              <  1:807977/150‑1 (MQ=255)
                                                                      tAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCga                                                                              <  1:559280/150‑1 (MQ=255)
                                                                      caaacggtAATGGCAAATGTCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCga                                                                              >  2:984076/8‑150 (MQ=255)
                                                                          atTTAATGACATTTGCCATTGCCGTTTGTGTTCAGGGGTGGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACAATCGTACCAGTGATTTCTCTATAACTATAGCTCACAGACCAAGTTAGCgaggag                                                                          >  2:287134/1‑150 (MQ=255)
                                                                           tttACTGACATTTGCCCTTACCGGTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGGCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGc                                                                         <  2:462312/150‑1 (MQ=255)
                                                                           ggtAATGGCAAATGTCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGc                                                                         >  1:539439/3‑150 (MQ=255)
                                                                            ttAATGACATTTGCCTTTACCGTTTGTGTTCAGGGGTCGTTATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCa                                                                        <  1:214957/150‑1 (MQ=255)
                                                                               aTGACATTTGCCATTACCGTTTGTGTACAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTAAAACTATAACTCACAGAACAACTTAGCGAGGAGCagac                                                                     >  1:1273095/1‑149 (MQ=255)
                                                                                   cATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGt                                                                 >  2:1311688/1‑150 (MQ=255)
                                                                                     tttGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGGCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTAc                                                               <  2:782305/150‑1 (MQ=255)
                                                                                         ccATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATc                                                           <  2:438606/150‑1 (MQ=255)
                                                                                         ccATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATc                                                           >  1:979803/1‑150 (MQ=255)
                                                                                           aTTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGt                                                         >  1:1128991/1‑150 (MQ=255)
                                                                                            ttACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTg                                                        <  1:1600150/150‑1 (MQ=255)
                                                                                             tACCGTTTGTGTGCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTga                                                       <  1:874915/150‑1 (MQ=255)
                                                                                               ccGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTgaga                                                     >  1:1070911/1‑150 (MQ=255)
                                                                                                cGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGaa                                                    >  1:966880/1‑150 (MQ=255)
                                                                                                cGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGaa                                                    >  1:1004233/1‑150 (MQ=255)
                                                                                                        ttCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTa                                            <  2:903173/150‑1 (MQ=255)
                                                                                                           aGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAg                                         <  2:1126601/150‑1 (MQ=255)
                                                                                                             gggTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAGCAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCa                                       >  2:1381427/1‑150 (MQ=255)
                                                                                                                 cGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCt                                   >  1:1259406/1‑150 (MQ=255)
                                                                                                                  gTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAACCCCATTAAATCACGCTg                                  >  2:881675/1‑150 (MQ=255)
                                                                                                                     aTGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCgagg                                                                            <  1:386072/105‑1 (MQ=255)
                                                                                                                     aTGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCgagg                                                                            >  2:386072/1‑105 (MQ=255)
                                                                                                                      tGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGt                              >  2:17067/1‑150 (MQ=255)
                                                                                                                       gAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGAGCGTGAGAAACCCATTTAAGCACGCTGCAGTg                             >  2:401370/1‑150 (MQ=255)
                                                                                                                            ccGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAGCTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCgtgt                        >  1:791916/1‑150 (MQ=255)
                                                                                                                             cGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTc                       >  2:956140/1‑150 (MQ=255)
                                                                                                                             cGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTc                       <  1:1168965/150‑1 (MQ=255)
                                                                                                                                 ttgttggtCGTTGAACAGGTGACGTCGCCATCTGTTAAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAACCATTAAAGCACTCTTCAGTGCGTGTCATTg                   <  1:842901/148‑1 (MQ=255)
                                                                                                                                  ggttggtCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGt                  >  1:1654201/1‑150 (MQ=255)
                                                                                                                                  ggttggtCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGt                  <  2:786310/150‑1 (MQ=255)
                                                                                                                                  ggttggtCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAACCATTAAAGCACTCTTCAGTTCGTGTCATTGt                  >  2:325459/1‑150 (MQ=255)
                                                                                                                                   gttggtAGTTGAACAGGTGACGGCGCCATCTGTTCAACAGTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGAAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGtt                 <  2:1054881/150‑1 (MQ=255)
                                                                                                                                   gttgggCGTTGAACAGGTGACGTCACCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGtt                 <  2:1207239/150‑1 (MQ=255)
                                                                                                                                      ggtCGTTGAACAGGTGACGTCACCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGc              <  1:1313275/150‑1 (MQ=255)
                                                                                                                                       gtCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGTACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGcc             <  1:193606/150‑1 (MQ=255)
                                                                                                                                        atgTTGAACAGATGGCGACGTCACCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCa            >  1:28720/3‑150 (MQ=255)
                                                                                                                                         cGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATCACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAg           <  2:598924/150‑1 (MQ=255)
                                                                                                                                            tGAACAGATGGCGACGTCACCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATg        >  2:1687360/1‑150 (MQ=255)
                                                                                                                                             gAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgc       >  2:1173030/1‑150 (MQ=255)
                                                                                                                                             gAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgc       >  1:239368/1‑150 (MQ=255)
                                                                                                                                              aaCAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTAAGCGAGGAGCAGATCGGTACGATCGAGAGAAACCCATTAAAGAACACTGCAGTGCGTATCATTGTTAGCAAGATgcg      >  2:711963/1‑150 (MQ=255)
                                                                                                                                               aCAGGTGACGTCGCTATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgcgg     >  2:1629485/1‑150 (MQ=255)
                                                                                                                                                 aGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgcggcg   <  2:1400030/150‑1 (MQ=255)
                                                                                                                                                  ggTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGACCAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGt  >  1:283449/1‑150 (MQ=255)
                                                                                                                                                  ggTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGt  <  2:539439/150‑1 (MQ=255)
                                                                                                                                                  ggTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGt  >  2:890653/1‑150 (MQ=255)
                                                                                                                                                  ggTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGt  >  1:106695/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGT  >  NC_000913/1357077‑1357372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: