Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,376,207 C→A 7.0% A58A (GCC→GCA ycjR → putative TIM alpha/beta barrel enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,2070CA 7.0% 309.9 / 9.3 107A58A (GCC→GCAycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base C (44/55);  new base A (4/4);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 107 total aligned reads displayed.

TCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTC  >  NC_000913/1376062‑1376354
                                                                                                                                                 |                                                                                                                                                   
tCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCg                                                                                                                                                 <  1:218694/150‑1 (MQ=255)
 cTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCgg                                                                                                                                                <  1:463129/150‑1 (MQ=255)
   ttCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACAAACGCCTGCGGTg                                                                                                                                              >  1:308804/1‑150 (MQ=255)
   ttCCGGAAAACATTCTGGAGAAATTTCGTAATATCCAAGAGATGGGCTTCGATGGTTTTGAGATTGCCGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGAGCAAAGAAACCGGCTTACCGGTGACCACCGCCTGCGGTg                                                                                                                                              <  1:44697/150‑1 (MQ=255)
     ccGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGTTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAATAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGc                                                                                                                                            <  2:740018/150‑1 (MQ=255)
     ccGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGCTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAACCGGCGAGCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGc                                                                                                                                            <  2:1364400/150‑1 (MQ=255)
      cGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCt                                                                                                                                           <  2:388815/150‑1 (MQ=255)
      cGGAAAACATTCTGGAGAAATTTCGTAATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCt                                                                                                                                           <  1:1112014/150‑1 (MQ=255)
      cGGAAAACATTATGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAATGAAACCGGTTTACGCGTGACCACCGCCTGCCGTGGCt                                                                                                                                           >  2:1081943/1‑150 (MQ=255)
       ggAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGCCGGCAAACTGCTGGTTAACAACATCGAAGAAGTAAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGACGTGGCTa                                                                                                                                          >  2:77058/1‑150 (MQ=255)
        gAAAACATTCTGGAGAATTTTCGTTATATCAAAGAGAGGGGCTTCGATGGGTTTGAGATTGACGTCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTAt                                                                                                                                         <  1:139335/150‑1 (MQ=255)
         aaaaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGTCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCAGGCGGTggtcacc                                                                                                                                        <  1:517552/150‑6 (MQ=255)
         aaaaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCAGGCGGTggtcacc                                                                                                                                        <  1:929219/150‑6 (MQ=255)
         aaaaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCAGGCGGTggtcacc                                                                                                                                        <  2:1561851/150‑6 (MQ=255)
           aaCATTCTGGAGAAATTTCTTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGAc                                                                                                                                      <  2:811490/150‑1 (MQ=255)
           aaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCAGGCGGTggtcaccgg                                                                                                                                      <  2:608658/150‑8 (MQ=255)
              aTTCTGGAGAAATTTCGTTATATCAAATCGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACgga                                                                                                                                   <  2:1556758/150‑1 (MQ=255)
               ttCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAAAAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACAGGTTTACAGGTGACCACCGCCTTCGGTGGCTATGACggat                                                                                                                                  >  1:1222024/1‑150 (MQ=255)
                    gagaAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGAtt                                                                                                                             <  2:1520304/150‑1 (MQ=255)
                      gaAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTgg                                                                                                                           <  1:1158094/150‑1 (MQ=255)
                        aaTTTCGTTCTATAAATGTGTTGGTCGTCGATGTTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGTAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCg                                                                                                                         <  2:296879/150‑1 (MQ=255)
                        aaTTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGTCAAACTGCTGGTTAACAACATCGAAGAAGTCGAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCg                                                                                                                         <  1:455784/150‑1 (MQ=255)
                        aaTTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACGACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCg                                                                                                                         <  1:1391673/150‑1 (MQ=255)
                        aaTTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCg                                                                                                                         <  2:1160988/150‑1 (MQ=255)
                                tataTCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGAAGGATTGGCGACTTTATc                                                                                                                 >  2:338657/1‑150 (MQ=255)
                                 atatCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGGTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCg                                                                                                                <  2:195193/150‑1 (MQ=255)
                                   atCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGCTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCCACTTTATCGaa                                                                                                              >  2:797641/1‑150 (MQ=255)
                                     cAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAaga                                                                                                            >  2:1389714/1‑150 (MQ=255)
                                       aaGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGc                                                                                                          <  2:1574856/150‑1 (MQ=255)
                                        agagATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcg                                                                                                         <  1:182718/150‑1 (MQ=255)
                                         gagaTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcgt                                                                                                        >  1:1722533/1‑150 (MQ=255)
                                           taTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcgtcg                                                                                                      <  1:1515180/149‑1 (MQ=255)
                                            aTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcgtcgt                                                                                                     >  2:1222631/1‑150 (MQ=255)
                                            aTGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcgtcgt                                                                                                     >  2:1432611/1‑150 (MQ=255)
                                             tGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCTAACAAAGAAACCGGTTTACCGGTGACCACCCCCTGCGGTGGCAAACACGGATGCATTCGCGACTTTATCGAAGAGCgtcttc                                                                                                    >  2:465044/1‑150 (MQ=255)
                                               ggCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCtt                                                                                                  <  2:674304/150‑1 (MQ=255)
                                                gCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTa                                                                                                 >  2:1409387/1‑150 (MQ=255)
                                                   tCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGCCGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATg                                                                                              >  1:1469598/1‑150 (MQ=255)
                                                   tCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATg                                                                                              <  1:956680/150‑1 (MQ=255)
                                                         gTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTaa                                                                                        >  1:1571277/1‑150 (MQ=255)
                                                         gTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTaa                                                                                        >  1:1171941/1‑150 (MQ=255)
                                                            ttGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGc                                                                                     >  1:695614/1‑150 (MQ=255)
                                                             tGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCa                                                                                    >  2:536472/1‑150 (MQ=255)
                                                              gagaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAg                                                                                   <  1:1634190/150‑1 (MQ=255)
                                                                gaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGAt                                                                                 <  1:1646174/150‑1 (MQ=255)
                                                                 aTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATc                                                                                >  2:1060021/1‑150 (MQ=255)
                                                                   tGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGa                                                                              <  2:811700/150‑1 (MQ=255)
                                                                    gACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAg                                                                             <  2:1571277/150‑1 (MQ=255)
                                                                    gACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAg                                                                             <  2:1171941/150‑1 (MQ=255)
                                                                         cAAAATGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATAGAGCGAAt                                                                        >  2:943666/1‑150 (MQ=255)
                                                                           aaCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCATATCGAGCGCATtc                                                                      >  2:2386/1‑150 (MQ=255)
                                                                            aCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATtct                                                                     <  1:448264/150‑1 (MQ=255)
                                                                                  ggTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAgc                                                               >  1:9082/1‑150 (MQ=255)
                                                                                    ttAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAgcgc                                                             >  1:579543/1‑150 (MQ=255)
                                                                                     tAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCt                                                            <  1:1670018/150‑1 (MQ=255)
                                                                                     tAACAACATCGAAGAAGGCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCt                                                            <  1:298378/150‑1 (MQ=255)
                                                                                      aacaacATCGAAGAAGTCAACGCGGCGATCAAAGAAAACGGTATACCGCTGACCACCGCCTTCGGAGGCTATGACGTATGGATTGGCGCCTTTATCGAAGAGCGTCGTCTGAATGGCTTAAAGCAGAACGAGCCCATTCTCGAAGCGCTg                                                           >  2:1513220/1‑150 (MQ=255)
                                                                                      aacaacATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATAGAAGAGCGTCGTCTTAATGGCTAAAAGCAGATCGAGCGCATTCTCGAAGCGCTg                                                           >  2:108616/1‑150 (MQ=255)
                                                                                      aacaacATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTCGCGACTTTATCGAAGAGCGTCGTCTTAATAGAATAAACAATAACTAACTCATTCTCGAAGCGCTg                                                           >  2:335522/1‑150 (MQ=255)
                                                                                        caacaTCGAAGAAGTCAAAGCGGCGATCAAATAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGc                                                         <  1:1409387/150‑1 (MQ=255)
                                                                                           cctCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGa                                                      <  1:150490/148‑1 (MQ=255)
                                                                                           caTCGAAGAAGTCATAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGa                                                      <  1:1017446/150‑1 (MQ=255)
                                                                                            aTCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGaa                                                     >  1:1561491/1‑150 (MQ=255)
                                                                                             tCGAAGAAGTCACAGCTGCGATCAAAGAAACCGGTGTACCGGTGACCACCGCCTGCGGTGGCCATGACGGATGGAGTGGCGACTTTATCGAAGAGCGGCGTCTTAATGTCGTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAg                                                    <  1:1066450/150‑1 (MQ=255)
                                                                                             tCGAAGAAGTCAAAGCGGCGATCAAAGAATCCGGTTTACCGTTGACCACCGCCTGCGGTGGCTATGACGGATGTATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAg                                                    <  1:1274622/150‑1 (MQ=255)
                                                                                               gaagaaGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTg                                                  <  1:869046/150‑1 (MQ=255)
                                                                                               gaagaaGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACGTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTg                                                  >  2:729652/1‑150 (MQ=255)
                                                                                                  gaagTCATCGCCGCGATCGAATAATCCGGTTTACCGGTGACCACCGCCTGCGTTGGTTATGACGGATGGTTTGGCGACATTATCGAAGAGGGGCGTCTTAATGGCATAAAGCAGAGCGACGGCTTTCTCGATTCTCGGGCAGatgctggc                                               <  2:470834/150‑8 (MQ=255)
                                                                                                   aagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCg                                              <  1:7357/150‑1 (MQ=255)
                                                                                                    agTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCgg                                             <  1:22176/150‑1 (MQ=255)
                                                                                                    agTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCgg                                             <  2:662688/150‑1 (MQ=255)
                                                                                                    agTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAAAGGCTTAAAGAAGATCGAGCGCATTCTCGAAGCGCGGGCAGAAGTGGGCgg                                             >  2:893612/1‑150 (MQ=255)
                                                                                                     gTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGt                                            >  1:1201542/1‑150 (MQ=255)
                                                                                                       cAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGACTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAAAGGCTTAAAGCAGATCGAGCGCAATCTCGAAGCGCTGGCAGAAGTCGCCGGTaa                                          >  2:362874/1‑150 (MQ=255)
                                                                                                           gcggcgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGc                                      >  1:833866/1‑150 (MQ=255)
                                                                                                           gcggcgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCGTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGc                                      >  2:342105/1‑150 (MQ=255)
                                                                                                               cgctCAAAGAAACCGGTGTCCCGGTGACCCCCGCCTGCGGTGGCTATGACGGATGGAGTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCGGGCAGAAGTGGGCGGTAAAGGCAtcg                                  <  1:279385/147‑1 (MQ=255)
                                                                                                               cgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtcg                                  <  1:283217/150‑1 (MQ=255)
                                                                                                               cgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtcg                                  >  1:1394678/1‑150 (MQ=255)
                                                                                                                  tCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtcgtcg                               >  1:513465/1‑150 (MQ=255)
                                                                                                                   cAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCGTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATcgtcgt                              >  1:1268864/1‑150 (MQ=255)
                                                                                                                    aaaGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGtt                             <  2:932447/150‑1 (MQ=255)
                                                                                                                     aaGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGCAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTc                            >  1:1225288/1‑150 (MQ=255)
                                                                                                                       gtcaCCGGTAAACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCg                          >  1:1129324/4‑150 (MQ=255)
                                                                                                                       gAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCg                          >  1:12634/1‑150 (MQ=255)
                                                                                                                               tttACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTg                  >  2:774328/1‑150 (MQ=255)
                                                                                                                                 tACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTggg                <  1:1060021/150‑1 (MQ=255)
                                                                                                                                  aCCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg               <  2:43210/150‑1 (MQ=255)
                                                                                                                                   ccGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCTTCGTCGTTCCGGCTGCGTGGGGc              >  1:1583593/1‑150 (MQ=255)
                                                                                                                                    cGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCa             <  2:1354035/150‑1 (MQ=255)
                                                                                                                                      atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGCGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGGAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATg           >  1:1406888/5‑150 (MQ=255)
                                                                                                                                      atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTGGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATg           >  1:1654838/5‑150 (MQ=255)
                                                                                                                                      atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTTATGGGTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATg           >  1:120669/5‑150 (MQ=255)
                                                                                                                                      atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGTCTTAAAGCATATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTTGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATg           >  2:1096854/5‑150 (MQ=255)
                                                                                                                                        agccaccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGtt         >  2:1660191/3‑150 (MQ=255)
                                                                                                                                         accaccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGttt        <  2:1722533/150‑1 (MQ=255)
                                                                                                                                          cgaccGCCTGCGGTGGATATGACGGATGGATTGGCGACTTTATCGTAGAGAGTGGTCTTAATGGCTTAAAGCAGAGCGAGCGCATTCTCGAAGCGCTGGGAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTa       <  1:327740/148‑1 (MQ=255)
                                                                                                                                            accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTAcc     >  1:1352855/1‑150 (MQ=255)
                                                                                                                                            accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTAcc     >  1:147346/1‑150 (MQ=255)
                                                                                                                                               gCCTGCGGTGGATATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTc  <  2:48304/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTC  >  NC_000913/1376062‑1376354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: