Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,386,750 C→G 7.7% L11V (CTC→GTC)  tyrR → aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,386,7500CG 7.7% 264.2 / 7.7 88L11V (CTC→GTC) tyrRaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase
Reads supporting (aligned to +/- strand):  ref base C (34/46);  new base G (3/2);  total (37/51)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

ACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACGCC  >  NC_000913/1386603‑1386897
                                                                                                                                                   |                                                                                                                                                    
aCGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGAGTc                                                                                                                                                    <  2:969478/150‑1 (MQ=255)
 cGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCg                                                                                                                                                   >  2:155701/1‑150 (MQ=255)
 cGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCg                                                                                                                                                   >  2:1345439/1‑150 (MQ=255)
 cGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCg                                                                                                                                                   >  2:1272333/1‑150 (MQ=255)
  gCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGGGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCgg                                                                                                                                                  <  1:1192317/150‑1 (MQ=255)
    cGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGTAAGTCTTTTGTGAAGACCGACTCGGTc                                                                                                                                                <  2:1331418/150‑1 (MQ=255)
        gTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGAc                                                                                                                                            <  1:1653020/150‑1 (MQ=255)
        gTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGAc                                                                                                                                            <  2:687100/150‑1 (MQ=255)
         tGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGAGTCGGTCttcac                                                                                                                                           <  1:1559937/150‑5 (MQ=255)
          gCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTTTGAAGACCGACTCGGTCTGAccc                                                                                                                                          >  1:1027824/1‑150 (MQ=255)
          gCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGAccc                                                                                                                                          >  2:1089412/1‑150 (MQ=255)
                 tttCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAAtt                                                                                                                                   <  2:987784/150‑1 (MQ=255)
                    ccGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACt                                                                                                                                <  1:203780/150‑1 (MQ=255)
                     cGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTc                                                                                                                               <  1:473787/150‑1 (MQ=255)
                     cGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTc                                                                                                                               <  1:433375/150‑1 (MQ=255)
                      gTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCg                                                                                                                              <  1:1647741/150‑1 (MQ=255)
                        cTTTGTGTCAATGATGGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGAt                                                                                                                            <  1:106860/150‑1 (MQ=255)
                          ttGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCt                                                                                                                          <  2:1340525/150‑1 (MQ=255)
                          ttGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCt                                                                                                                          <  1:1272333/150‑1 (MQ=255)
                          ttGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCt                                                                                                                          <  2:79797/150‑1 (MQ=255)
                               tCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCg                                                                                                                     >  2:941100/1‑150 (MQ=255)
                                cAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGt                                                                                                                    >  1:902534/1‑150 (MQ=255)
                                   tGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGACTGACCCGCGAATTACTCGATCTACTCGTGCt                                                                                                                 >  1:1696673/1‑150 (MQ=255)
                                    gATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTa                                                                                                                <  1:14158/150‑1 (MQ=255)
                                    gATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTa                                                                                                                <  1:1262906/150‑1 (MQ=255)
                                      ttgttgACAGAAACCTTCCTGCTATCAAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGTAGTCTTTTGTGATGACCGACTCTGTCTGACCAGCGATTTACTCGATCTACTCGTGCTaat                                                                                                              >  1:235116/1‑149 (MQ=255)
                                         ttgACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAgg                                                                                                           <  1:1544398/150‑1 (MQ=255)
                                         ttgACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGGTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAgg                                                                                                           <  1:187749/150‑1 (MQ=255)
                                          tgACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGCCCGACTCGGTCTGACCCGCGAATTACTAGATCTACTCGTGCTAAGAGGc                                                                                                          >  2:76839/1‑150 (MQ=255)
                                           gACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCa                                                                                                         >  2:844420/1‑150 (MQ=255)
                                           gACAGAAACCTTCCTGCTATACAAATAGTGTCATATCATCAAATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTTATTTTTTTTTTTTTTTTTACGGCGAATTACTAGATCTACTCGTGCTAAGAGGCa                                                                                                         <  2:968063/150‑1 (MQ=255)
                                             cAGAAAGCTTCCTGCTATCCAAATAGTGTCATATCATCATATTATTTGTTCTTTTTTCAGGTGAAGGTTCGCATGCTTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCAtt                                                                                                       <  1:20885/150‑1 (MQ=255)
                                             cAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCAtt                                                                                                       <  1:1393048/150‑1 (MQ=255)
                                              aGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTg                                                                                                      <  2:1009951/150‑1 (MQ=255)
                                               gAAACCTTCCTGCTATCCAAATAGTGTCATATAATCATATTAATTGTTCTTTTTTAAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGAACGACTCGGTCTGAACCGCGAATTACTCGATATACTCGTGCTAATAGGAATTGa                                                                                                     >  1:180258/1‑150 (MQ=255)
                                                 aaCCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGAtt                                                                                                   >  2:945634/1‑150 (MQ=255)
                                                       ccTGCTATCCAAATAGAGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTAcgcg                                                                                             <  1:210901/150‑1 (MQ=255)
                                                          gCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTa                                                                                          >  2:1596571/1‑150 (MQ=255)
                                                          gCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATATACTAGTGCTAAGAGGCATTGATTTACGCGGTa                                                                                          >  1:41749/1‑150 (MQ=255)
                                                              accAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTga                                                                                      <  2:660760/149‑1 (MQ=255)
                                                               acAACTAGTGTCATTTCATCATATTAATTGTTCTTTTTTCAGGGGAAGGTTCACATGCGTCTGGAAGTGTTATGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTgtg                                                                                     <  2:1540638/149‑3 (MQ=255)
                                                                cAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTgaga                                                                                    >  2:1107773/1‑150 (MQ=255)
                                                                    tAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGACTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGa                                                                                <  2:1696673/150‑1 (MQ=255)
                                                                     aGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCCTGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGAt                                                                               >  2:255732/1‑150 (MQ=255)
                                                                     aGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGAt                                                                               <  1:155701/150‑1 (MQ=255)
                                                                      gtgtCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATc                                                                              <  1:945634/150‑1 (MQ=255)
                                                                        gtCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATccc                                                                            <  1:1663315/150‑1 (MQ=255)
                                                                        gtCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATcca                                                                            >  1:1078158/1‑149 (MQ=255)
                                                                            tatCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTTAAGATATATTATTTTTTAACCGCGAATTACTAGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTg                                                                        <  2:1291323/150‑1 (MQ=255)
                                                                                   atatTACTTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGGCTGGAAGTCTTTTCTGAAGACCGACTCGGGCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAAt                                                                 <  2:92979/150‑1 (MQ=255)
                                                                                   atatTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACAGACTCGGTCTGAACCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAAt                                                                 <  2:1310904/150‑1 (MQ=255)
                                                                                    tatTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCTGTATTGAGATTGATCCCATTGGGCGAATc                                                                >  2:943644/1‑150 (MQ=255)
                                                                                            gTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCaa                                                        >  2:327390/1‑150 (MQ=255)
                                                                                              tCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAAtt                                                     <  1:1422257/150‑1 (MQ=255)
                                                                                               cTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGTCGAATCTACCTCAAttt                                                    >  2:1153555/1‑150 (MQ=255)
                                                                                               cTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAAttt                                                    >  2:686852/1‑150 (MQ=255)
                                                                                                  ttttCAGGTTAAGGGTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGTTCTGACCCGAGAATTACTCGATCTACTCGTGCTAACAGGCATTGATTTACGCGGTATTTAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGc                                                 <  2:116771/150‑1 (MQ=255)
                                                                                                  ttttCAGGTGAAGGTTCCCAAGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGc                                                 <  2:519327/150‑1 (MQ=255)
                                                                                                   tttCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCt                                                >  2:871493/1‑150 (MQ=255)
                                                                                                     tCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGa                                              >  2:356756/1‑150 (MQ=255)
                                                                                                          tGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGAGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTgg                                         >  1:1693552/1‑150 (MQ=255)
                                                                                                            aaGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAg                                       <  1:941100/150‑1 (MQ=255)
                                                                                                            aaGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAg                                       <  1:1345439/150‑1 (MQ=255)
                                                                                                               gTTCCCATGCGTCTGGAAGTCTTTTGTGAAGTTTTTTTTTTTTTTAACGGCGAATTACTAGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGttt                                    <  2:574604/150‑1 (MQ=255)
                                                                                                               gTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGttt                                    <  2:689693/150‑1 (MQ=255)
                                                                                                                ttCCCATGCGTCTGGAAGTCTGTTGTGAAGACCGACTCGGGCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTg                                   <  1:1354901/150‑1 (MQ=255)
                                                                                                                       gCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCTAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGttt                            <  2:835797/150‑1 (MQ=255)
                                                                                                                        cGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAATAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTc                           <  1:1089412/150‑1 (MQ=255)
                                                                                                                        cGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGATGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTc                           <  2:1027824/150‑1 (MQ=255)
                                                                                                                         gTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTca                          >  2:677748/1‑150 (MQ=255)
                                                                                                                           cTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTcagc                        <  1:844420/150‑1 (MQ=255)
                                                                                                                              gAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGt                     >  2:361078/1‑150 (MQ=255)
                                                                                                                              gAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGt                     >  1:90470/1‑150 (MQ=255)
                                                                                                                                aGTCTTTTGTGAAGACCGACTCGGTCTGTGCCGCTAATTACTCGATCTTCTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTTCCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCt                   <  1:76839/150‑1 (MQ=255)
                                                                                                                                   cTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGa                 <  2:618389/150‑1 (MQ=255)
                                                                                                                                       tgtgAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGcc            <  2:1489794/150‑1 (MQ=255)
                                                                                                                                       gggtcaGACCGAGTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGcc            >  2:1389707/6‑150 (MQ=255)
                                                                                                                                       gggtcaGACCGAGTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGcc            >  2:1259418/6‑150 (MQ=255)
                                                                                                                                        gtgAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCg           >  2:1375224/1‑150 (MQ=255)
                                                                                                                                        ggtcaGACCGAGTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCg           >  1:232636/5‑150 (MQ=255)
                                                                                                                                          gAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGCGTTTCAGCAGTCTGATGGCCGaa         >  2:152372/1‑150 (MQ=255)
                                                                                                                                          gAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGaa         >  2:1028295/1‑150 (MQ=255)
                                                                                                                                            aGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAAt       <  2:902534/150‑1 (MQ=255)
                                                                                                                                             gACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATa      <  2:1492904/150‑1 (MQ=255)
                                                                                                                                               ccGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACg    <  1:1493259/150‑1 (MQ=255)
                                                                                                                                                 gACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTAGGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACgac  >  1:429303/1‑148 (MQ=255)
                                                                                                                                                 gACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACGcc  >  2:386322/1‑150 (MQ=255)
                                                                                                                                                 gACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCAACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACGcc  >  1:872742/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCCGAAGTGCCCGTTTTTCCGTCTTTGTGTCAATGATTGTTGACAGAAACCTTCCTGCTATCCAAATAGTGTCATATCATCATATTAATTGTTCTTTTTTCAGGTGAAGGTTCCCATGCGTCTGGAAGTCTTTTGTGAAGACCGACTCGGTCTGACCCGCGAATTACTCGATCTACTCGTGCTAAGAGGCATTGATTTACGCGGTATTGAGATTGATCCCATTGGGCGAATCTACCTCAATTTTGCTGAACTGGAGTTTGAGAGTTTCAGCAGTCTGATGGCCGAAATACGCC  >  NC_000913/1386603‑1386897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: