Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,408,537 A→T 7.8% E163V (GAG→GTG)  ydaN → putative Zn(II) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,408,5370AT 7.8% 189.2 / 7.5 77E163V (GAG→GTG) ydaNputative Zn(II) transporter
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base T (2/4);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-02

CGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCA  >  NC_000913/1408389‑1408686
                                                                                                                                                    |                                                                                                                                                     
cGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTCGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGag                                                                                                                                                      <  2:1645866/150‑1 (MQ=255)
  gTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagca                                                                                                                                                    >  2:1648165/1‑150 (MQ=255)
  gTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACAGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagca                                                                                                                                                    >  1:1609243/1‑150 (MQ=255)
    tAATTGTTTCGACCCGACAACGCAAAGTGCTCGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGagcagc                                                                                                                                                  <  1:615315/150‑1 (MQ=255)
      aTTGATTCTACCCGACAACGCAAAGTGCGGGCGCTGGACGATGAGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGTCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTg                                                                                                                                                <  2:861140/150‑1 (MQ=255)
          ttgcgacccgaccACGCAAAGTGCTGGCGCTGGACGATGTGGGGAGCGATCTGGAAGCGGGCACGGGTCCGACCGATTGCGGGGGATGGCGGGGGGATGTGGGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACg                                                                                                                                            <  1:1580382/147‑1 (MQ=255)
           ttCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGa                                                                                                                                           >  1:540813/1‑150 (MQ=255)
             cGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCCCGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATa                                                                                                                                         >  2:755598/1‑150 (MQ=255)
              gACCCGACAACGCACAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                        <  1:1478172/150‑1 (MQ=255)
              gACCCGACAACGCAAAGGCCTGCCGCTGGACGATGTGGCTAGCGTTCTGGAAGCTGGCACGGGTCCTACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATaa                                                                                                                                        <  2:1106476/150‑1 (MQ=255)
                cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaat                                                                                                                                      <  1:1031618/150‑2 (MQ=255)
                cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaat                                                                                                                                      <  2:1443760/150‑2 (MQ=255)
                cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaat                                                                                                                                      <  2:1694365/150‑2 (MQ=255)
                cccGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGAGCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGTGCAGCTGCTCGATaaat                                                                                                                                      <  2:1481265/150‑2 (MQ=255)
                     cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATc                                                                                                                                 <  2:1438888/150‑1 (MQ=255)
                     cAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATc                                                                                                                                 <  1:421897/150‑1 (MQ=255)
                     aaaCGCAAAGTGCTGGCGATGGACGATGTGGTGAGCTATCTGTAAGAGGGCACGGGTCCGACCGATTGCGGGTGATGGCTGGTGGATGTGTTCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATc                                                                                                                                 <  2:1191716/149‑1 (MQ=255)
                      aaCGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGAGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCg                                                                                                                                >  1:1471090/1‑150 (MQ=255)
                        cGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAc                                                                                                                              >  1:499620/1‑150 (MQ=255)
                        cGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAc                                                                                                                              >  1:1386818/1‑150 (MQ=255)
                        cGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAc                                                                                                                              >  2:65308/1‑150 (MQ=255)
                         gCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGAcc                                                                                                                             <  2:1394610/150‑1 (MQ=255)
                           aaaGTGCTGGCGCTGGACGATGTGGTGACCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCTAGCAGCTGCACGATAAAATTATCGACAtt                                                                                                                           >  2:1068421/1‑150 (MQ=255)
                            aaGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTg                                                                                                                          <  1:1387304/150‑1 (MQ=255)
                                gCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGa                                                                                                                      >  1:1071767/1‑150 (MQ=255)
                                  tGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGCCCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATa                                                                                                                    >  1:175977/1‑150 (MQ=255)
                                    gctcTGGACGATGTGGTGAGCGATCTGGAAGAGGGAACGGTTACGACCGATTGCGGGGGATGGATTATGTATGTGTGCGATGCGTTGAACGATCAAACCAGAGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAt                                                                                                                  >  2:228006/4‑150 (MQ=255)
                                      gcTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAtct                                                                                                                >  1:1461734/1‑150 (MQ=255)
                                      gcTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAAtat                                                                                                                >  1:1064795/1‑148 (MQ=255)
                                           aCGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCTAGCAGCTGCACGATAAAATTATCGAACTTGAAGATAATCTCCttt                                                                                                           >  1:758264/1‑149 (MQ=255)
                                            cGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGa                                                                                                          >  1:1382615/1‑150 (MQ=255)
                                                   gtgAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCaa                                                                                                   <  1:1150616/150‑1 (MQ=255)
                                                           tCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCAcc                                                                                           >  1:1695244/1‑150 (MQ=255)
                                                            cTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACcg                                                                                          >  1:1275838/1‑150 (MQ=255)
                                                                 agagGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGTGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTgg                                                                                     <  2:1650596/150‑1 (MQ=255)
                                                                     ggCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTc                                                                                 >  1:1446346/1‑150 (MQ=255)
                                                                      ccACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTcc                                                                                >  1:386064/2‑150 (MQ=255)
                                                                               cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgc                                                                       <  1:1611887/150‑1 (MQ=255)
                                                                               cgaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATATATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgc                                                                       >  1:689686/1‑150 (MQ=255)
                                                                                gaccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgct                                                                      >  2:187753/1‑150 (MQ=255)
                                                                                 accgaTTGCGGGGGATGGCTGGGGGATGTGTGCGATGCGATGACCGATCATTCCAGTTAATTTATCGAGCAGCTGCACGATAAAATTATCGACCATGAAGATAATCTCATTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTctgctg                                                                     <  2:349169/150‑1 (MQ=255)
                                                                                  ccgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCtgctgc                                                                    <  1:596376/150‑1 (MQ=255)
                                                                                   cgaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTgcg                                                                   >  2:1211320/1‑150 (MQ=255)
                                                                                    gaTTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTgcgc                                                                  >  1:302220/1‑150 (MQ=255)
                                                                                        gCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGATCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAAc                                                              <  1:774610/150‑1 (MQ=255)
                                                                                        gCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAAc                                                              >  1:518609/1‑150 (MQ=255)
                                                                                            gggATGGCTGGTTGATGTGTGCGATGCGTTGACCGATCATTCCAGTCAATTTCTCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaatt                                                          <  2:1167116/150‑1 (MQ=255)
                                                                                             ggATGGCTGGTGGATGGGTGCGATGCGTTGACCGATCAATCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaatta                                                         <  2:892269/150‑1 (MQ=255)
                                                                                              gATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaa                                                        >  2:1413162/1‑150 (MQ=255)
                                                                                                tGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACaattaatt                                                      <  2:1461734/150‑1 (MQ=255)
                                                                                                   cTGGTGGATGTGTGCGATGCGTTGTACGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgtg                                                   <  2:919291/150‑1 (MQ=255)
                                                                                                   cTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATtgtt                                                   >  2:1356159/1‑149 (MQ=255)
                                                                                                    tggtggATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCTCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGa                                                  <  1:1032273/150‑1 (MQ=255)
                                                                                                    tggtggATGGGTGCGATTCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTAGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGa                                                  <  1:1523697/150‑1 (MQ=255)
                                                                                                           tgtgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCg                                           <  2:518609/150‑1 (MQ=255)
                                                                                                             tgtgCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCt                                         >  1:648278/1‑150 (MQ=255)
                                                                                                                  gATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATg                                    >  2:748000/1‑150 (MQ=255)
                                                                                                                  gATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATg                                    <  1:417110/150‑1 (MQ=255)
                                                                                                                  gATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATg                                    >  2:741731/1‑150 (MQ=255)
                                                                                                                    tGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGc                                  <  1:1356269/150‑1 (MQ=255)
                                                                                                                    tGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGc                                  <  2:436858/150‑1 (MQ=255)
                                                                                                                      cGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCAc                                >  2:531526/1‑150 (MQ=255)
                                                                                                                          gACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCa                            >  2:461405/1‑150 (MQ=255)
                                                                                                                           aCCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTACACAGCGTGGATTCATGGCTCTGCTGCTCAAACAATTAATTCTGCTGCGACGCTATATGGCACAGCaa                           >  1:153626/1‑150 (MQ=255)
                                                                                                                            ccGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATATTCTCCTTGATCAGCAAAATCCACCGCGTGGATTGCTGGCTCTGCTGAGCAATGTATTAATTGTGATGCGTCGCTGTATGGCAGCggacc                          >  2:1053660/1‑146 (MQ=255)
                                                                                                                            ccGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAAc                          >  2:699238/1‑150 (MQ=255)
                                                                                                                                  aTTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATg                    <  2:1476504/150‑1 (MQ=255)
                                                                                                                                    tCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGtt                  <  2:1071767/150‑1 (MQ=255)
                                                                                                                                     ccAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGttt                 <  2:1723629/150‑1 (MQ=255)
                                                                                                                                      cAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATAAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTa                <  2:175977/150‑1 (MQ=255)
                                                                                                                                         taattttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGc             >  2:1310824/5‑150 (MQ=255)
                                                                                                                                           attttATCGTGCAGCTGCTCGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTc           >  1:628928/3‑150 (MQ=255)
                                                                                                                                           aaTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCATATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCtg           <  1:228006/150‑2 (MQ=255)
                                                                                                                                           aaTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTc           >  2:1268451/1‑150 (MQ=255)
                                                                                                                                            aTTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGAAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCg          <  2:625510/150‑1 (MQ=255)
                                                                                                                                               tATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCt       <  2:1471090/150‑1 (MQ=255)
                                                                                                                                                aTCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCtt      >  2:818863/1‑150 (MQ=255)
                                                                                                                                                 tCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTg     <  2:194152/150‑1 (MQ=255)
                                                                                                                                                  cGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGc    >  1:850232/1‑150 (MQ=255)
                                                                                                                                                   gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGcc   >  1:198955/1‑150 (MQ=255)
                                                                                                                                                   gAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGcc   <  2:1695244/150‑1 (MQ=255)
                                                                                                                                                    agcagcTGCACGATAAAATTATCGACCTTGAAGATAATCTACTTGATCTGCAAATTCCACCGCGTTGATTCCTGGCTCTGCTGCGCATACAATTAATTGTGATGCTTCGCTATATGGCACCTCAACGTGATGTTTATGCTCGTCTTGCCa  <  1:1624051/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGTTAATTGTTTCGACCCGACAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCGACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAATTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAAATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTATATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCA  >  NC_000913/1408389‑1408686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: