Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,441,837 G→C 7.5% intergenic (‑94/+17) hslJ ← / ← ldhA heat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,441,8370GC 7.5% 359.9 / 17.6 120intergenic (‑94/+17)hslJ/ldhAheat‑inducible lipoprotein involved in novobiocin resistance/fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (58/52);  new base C (4/5);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

Only 100 of 120 total aligned reads displayed.

TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAC  >  NC_000913/1441690‑1441976
                                                                                                                                                   |                                                                                                                                           
tacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTATGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCGGGAATGCa                                                                                                                                           >  2:82650/1‑150 (MQ=255)
tacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCa                                                                                                                                           >  1:428110/1‑150 (MQ=255)
tacACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCa                                                                                                                                           >  1:369611/1‑150 (MQ=255)
 acacATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAg                                                                                                                                          <  1:1545001/150‑1 (MQ=255)
  cacaTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAgg                                                                                                                                         <  2:482212/150‑1 (MQ=255)
    caTCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAgggg                                                                                                                                       >  2:840060/1‑150 (MQ=255)
       cccGCCATCAGCAGGCTTTGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTAGTTTAAGAATAGAGGATGAAAGGTCTTTGGGGTTTATCTGAATCAGCTCACAGGGAATGCAGGGGAGc                                                                                                                                    >  2:1642975/1‑150 (MQ=255)
       cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACTGTTTCTGACAAAGAACTATTTTAAGAATAGAGGATGAAAGGTAATTGTGGATTAACTGAATCAGCTCCCCTGGAATGCAGGGgcgc                                                                                                                                    >  2:825571/1‑150 (MQ=255)
       cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                                                                    <  2:947093/150‑1 (MQ=255)
       cccGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGCATTCCAGGGGAGc                                                                                                                                    <  1:1645523/150‑1 (MQ=255)
         cGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCACGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                                                                  <  1:1537749/150‑1 (MQ=255)
         cGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCAGTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCgg                                                                                                                                  >  1:1153939/1‑150 (MQ=255)
             aTCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAg                                                                                                                              <  1:1009128/150‑1 (MQ=255)
               cagcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAt                                                                                                                            >  2:551035/1‑150 (MQ=255)
                agcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGAtt                                                                                                                           <  1:701677/150‑1 (MQ=255)
                 gccggCTTAGCTCAACAAACGAGGCTACTTTCTTCATTGTGGAGCTCAATTAAATTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCCTTGGGGATTATCTGACTCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTa                                                                                                                          <  2:346988/147‑1 (MQ=255)
                 gcagGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTa                                                                                                                          >  2:1215484/1‑150 (MQ=255)
                   agGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGGGCGGCAAGATTaaa                                                                                                                        <  1:1171488/150‑1 (MQ=255)
                    ggCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTAATTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAAc                                                                                                                       <  1:1233132/150‑1 (MQ=255)
                       ttAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAg                                                                                                                    <  1:511111/150‑1 (MQ=255)
                         aGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                                                                  <  2:909983/150‑1 (MQ=255)
                         aGCGCAACAAACGCGGCTACTTTCATCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgtt                                                                                                                  >  1:662735/1‑150 (MQ=255)
                          gcgcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGTGAGCGGCAAGATTAAACCAgttc                                                                                                                 >  2:856521/1‑150 (MQ=255)
                          gcgcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttc                                                                                                                 >  2:1518601/1‑150 (MQ=255)
                           cgcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTTAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAggtcg                                                                                                                >  2:1504506/1‑150 (MQ=255)
                           cgcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcg                                                                                                                <  1:133623/150‑1 (MQ=255)
                            gcAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAgttcgt                                                                                                               >  1:1299219/1‑150 (MQ=255)
                                    cgcgGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAg                                                                                                       <  2:1094621/150‑1 (MQ=255)
                                       ggCTACTTTCTTCATTGTGGTTATCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGtt                                                                                                    >  1:1037889/1‑150 (MQ=255)
                                            cTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGcc                                                                                               >  2:1103690/1‑150 (MQ=255)
                                             tttCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTATAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGTGCAGGTTTCGCCt                                                                                              <  2:1295955/150‑1 (MQ=255)
                                             tttCTTCATTGTGGTTCACAATTACAGATTCTGACTCAGGACTATTTTAAGAATAGAGGATGCAAGGTCATTGGGGATTATCTGAAGCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCt                                                                                              <  2:1295958/150‑1 (MQ=255)
                                                cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCtttt                                                                                           >  1:402749/1‑150 (MQ=255)
                                                cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCtttt                                                                                           <  1:1655908/150‑1 (MQ=255)
                                                cttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGGCATTGGGGATTATCTGAATCAGCTCCCCTGGAATACAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCtttt                                                                                           <  1:332043/150‑1 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                                                        <  2:1589497/150‑1 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATGGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                                                        <  1:707710/150‑1 (MQ=255)
                                                   cATTGTGGTTCTCAATTACAGTTTCTGACTCACGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGACTCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTcc                                                                                        <  1:1199604/150‑1 (MQ=255)
                                                     ttGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                      <  2:1708026/150‑1 (MQ=255)
                                                     atgtgGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAg                                                                                      <  2:1245767/149‑1 (MQ=255)
                                                        tgGTTCTCAATTACCGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGAtt                                                                                   <  2:715633/150‑1 (MQ=255)
                                                          gtgctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGc                                                                                 <  2:457255/147‑1 (MQ=255)
                                                          gTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGc                                                                                 <  2:428110/150‑1 (MQ=255)
                                                            tctcAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCtt                                                                               >  1:1259083/1‑150 (MQ=255)
                                                               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                                                            <  1:819038/150‑1 (MQ=255)
                                                               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGAAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                                                            >  2:1064768/1‑150 (MQ=255)
                                                               cAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGAGGACAGGGCATTTGGGATTAGCTGAATCAGCTCCCCTGGAATGCTGGGGAGCGGCAAGATTAACCCAGGTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAg                                                                            <  1:215020/150‑1 (MQ=255)
                                                                 aTTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAACCCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGtt                                                                          >  2:234505/1‑150 (MQ=255)
                                                                     cAGTTTCTGACTCAGGACTATTTTAATAATAGAGGATGAAAGGTCATTGGTGTTTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGc                                                                      <  1:1573386/150‑1 (MQ=255)
                                                                       gTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAg                                                                    >  2:416719/1‑150 (MQ=255)
                                                                         ttCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCg                                                                  <  1:463222/150‑1 (MQ=255)
                                                                                tCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                           <  2:29143/150‑1 (MQ=255)
                                                                                    gACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGaa                                                       >  1:1010602/1‑150 (MQ=255)
                                                                                      cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGATCGGCAAGATTAAACCAGTTAGTTCGGGCAGGATTCGCCTTTTACAAGATTGATAAAGTTTTGCAGCGTAGTCTGAGAAAt                                                     >  2:320811/1‑150 (MQ=255)
                                                                                      cTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAt                                                     >  2:672522/1‑150 (MQ=255)
                                                                                        aTTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCACCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTTAGTTTTGCAGCGTAGTCTGAGAAAttc                                                   >  1:258293/1‑148 (MQ=255)
                                                                                         ttttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACt                                                  >  2:1165069/1‑150 (MQ=255)
                                                                                           ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg                                                >  1:1092923/1‑150 (MQ=255)
                                                                                           ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTgg                                                >  2:1646052/1‑150 (MQ=255)
                                                                                             aaGATTAGACGATGAAATGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTc                                              <  1:955407/150‑1 (MQ=255)
                                                                                              aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa                                             >  2:1263065/1‑150 (MQ=255)
                                                                                              aGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCa                                             <  1:551035/150‑1 (MQ=255)
                                                                                                  tAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTGCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGc                                         <  1:502052/150‑1 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTATGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                        >  1:901306/1‑150 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                        <  2:662735/150‑1 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGGCTGAGAAATACTGGTCAGAGCt                                        <  1:916437/150‑1 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGATTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCt                                        >  2:82541/1‑150 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGATTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                           <  1:299162/131‑1 (MQ=255)
                                                                                                   agagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGATTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTg                                                           >  2:299162/1‑131 (MQ=255)
                                                                                                      ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTct                                     <  1:642494/150‑1 (MQ=255)
                                                                                                        aTGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTctgc                                   <  1:1263065/150‑1 (MQ=255)
                                                                                                             aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCa                              >  1:1409436/1‑150 (MQ=255)
                                                                                                             aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCa                              <  1:1720078/150‑1 (MQ=255)
                                                                                                             aGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGATTAATTTTTGCAGAGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCa                              >  1:1053261/1‑150 (MQ=255)
                                                                                                               gTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAgg                            >  2:1728584/1‑150 (MQ=255)
                                                                                                                      gggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCt                     <  1:972974/150‑1 (MQ=255)
                                                                                                                       ggATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTg                    >  2:1377926/1‑150 (MQ=255)
                                                                                                                        gATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTgg                   <  1:1728584/150‑1 (MQ=255)
                                                                                                                          ttATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTg                 <  2:775210/150‑1 (MQ=255)
                                                                                                                          taaTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTg                 <  1:180736/148‑1 (MQ=255)
                                                                                                                            aTCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGATTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGcc               <  1:82541/150‑1 (MQ=255)
                                                                                                                             tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGccc              <  2:709087/150‑1 (MQ=255)
                                                                                                                             tCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGccc              >  1:1390121/1‑150 (MQ=255)
                                                                                                                              cTGAATCAGCTCCCCTGGAATGCAGGGGACCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTATGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGACCg             >  2:799161/1‑150 (MQ=255)
                                                                                                                               tGAATCCGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCgg            >  1:1175824/1‑150 (MQ=255)
                                                                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCGTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCgg            >  1:1512874/1‑150 (MQ=255)
                                                                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCgg            >  1:1238609/1‑150 (MQ=255)
                                                                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCgg            >  1:1519443/1‑150 (MQ=255)
                                                                                                                               tGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAACATTAAACCAGATCGTTCGGGCAGGTTTCGCCTATTTCCAGATTGCTTAAGTATAGCAGCGTAGTCTGAGATATACTGGTCAGAGCTTCTGCTGTCAGGAATGACTGGTGCACgg            >  2:231489/1‑150 (MQ=255)
                                                                                                                                  tgccgCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGTGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaa         >  1:753319/5‑150 (MQ=255)
                                                                                                                                  aTCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTaa         >  1:755597/1‑150 (MQ=255)
                                                                                                                                    ccgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGCCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGACAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc       >  1:1214632/3‑150 (MQ=255)
                                                                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc       >  2:244305/1‑150 (MQ=255)
                                                                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc       <  2:402749/150‑1 (MQ=255)
                                                                                                                                    cAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGAAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAc       >  2:889715/1‑150 (MQ=255)
                                                                                                                                     cgCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGTTAAACa      >  1:935272/2‑150 (MQ=255)
                                                                                                                                      gCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTGGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAg     >  1:483533/1‑150 (MQ=255)
                                                                                                                                      gCTCCCCTGCATTCCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTCGTGCCCGGTAAACAg     >  1:203440/1‑150 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTGCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAc  <  1:840060/150‑1 (MQ=255)
                                                                                                                                         ccccTGGAATGCAGGGGAGCGGCAAGAGTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAc  <  2:350426/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
TACACATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGACTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCAC  >  NC_000913/1441690‑1441976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: