| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 134,166 | A→G | 5.0% | Q851R (CAG→CGG) | acnB → | aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 134,166 | 0 | A | G | 5.0% | 308.6 / 4.7 | 101 | Q851R (CAG→CGG) | acnB | aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (49/46); new base G (3/2); total (52/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
GAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCC > NC_000913/134017‑134315 | gAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCa > 1:378734/1‑150 (MQ=255) aTGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGCCGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACATACCGTTATCTGAACTTCAACCATCTTTCTCAGt > 2:758980/1‑150 (MQ=255) tGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTa > 1:1679836/1‑150 (MQ=255) gTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTac > 2:1149609/1‑150 (MQ=255) tCCTGGCTTCTGCGGAACTGGCGGCTGGTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg < 1:1450911/150‑1 (MQ=255) ccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCga > 1:1382364/1‑150 (MQ=255) ccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCga > 1:1383372/1‑150 (MQ=255) cTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgag > 1:748707/1‑150 (MQ=255) gctgctGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCAGTAAGAGTACCATACATAAGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGaaa < 1:28263/150‑1 (MQ=255) cTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAg > 2:1561917/1‑150 (MQ=255) tCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCTGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGcc < 2:1676086/150‑1 (MQ=255) tCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGct < 1:1462913/150‑2 (MQ=255) cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGctg < 1:701284/150‑3 (MQ=255) cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg > 2:1718569/1‑150 (MQ=255) gAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATggg < 2:1079966/150‑1 (MQ=255) gAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATggg > 1:810105/1‑150 (MQ=255) aCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGt < 1:1110621/150‑1 (MQ=255) cTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTg > 1:1183386/1‑150 (MQ=255) cTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTg < 1:891506/150‑1 (MQ=255) tGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACCCCGAGAAAGCCGATGGGGTGa > 2:1577093/1‑150 (MQ=255) tGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGa > 1:647247/1‑150 (MQ=255) ggcggcTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGAt > 1:670900/1‑150 (MQ=255) tGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCa < 2:588421/150‑1 (MQ=255) gcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGt < 2:1374776/150‑1 (MQ=255) gcTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGt > 2:927260/1‑150 (MQ=255) cTGATTGGCAAACTGCCGACCCCGGAAGAGTACCAGACCTACGTGGCGCAGCTAGATAAAACAGCCGTAGATACTTCACGATATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAAGTGGTGATTTTCCAGAATGCGGtt > 1:284426/1‑150 (MQ=255) ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGACGGTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaa < 1:97642/150‑1 (MQ=255) ggCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaaa < 1:29524/150‑1 (MQ=255) ggCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaaa < 1:61322/150‑1 (MQ=255) gCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGGGGCGCAGGTAGATAAAACAGCCGGTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAg < 2:826171/150‑1 (MQ=255) cTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgc > 1:578372/1‑150 (MQ=255) gCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgcgc > 1:1249267/1‑150 (MQ=255) gACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCTTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAcg < 2:161087/150‑1 (MQ=255) cGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGAGCAcgag > 1:13162/1‑148 (MQ=255) cGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGACAGCGCAAGCGCTg > 1:93628/1‑150 (MQ=255) ggAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgc > 1:1052829/1‑150 (MQ=255) ggAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgc < 1:1208136/150‑1 (MQ=255) ggAAGAGTACCAGACCAACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTATACAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgc > 1:96980/1‑150 (MQ=255) agagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCa > 2:1472789/1‑150 (MQ=255) gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCCGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt < 1:958181/150‑1 (MQ=255) gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt < 1:1561917/150‑1 (MQ=255) gagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCAt < 1:913735/150‑1 (MQ=255) aCCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCATCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAAc < 1:17352/150‑1 (MQ=255) aCCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAAc > 1:1326567/1‑150 (MQ=255) aCCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACATCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATTGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATATAc > 2:927156/1‑150 (MQ=255) ccTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGAcaca > 2:171090/1‑150 (MQ=255) cGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGc > 1:381058/1‑150 (MQ=255) ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg < 1:75611/150‑1 (MQ=255) gcgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCgg < 2:1195466/150‑1 (MQ=255) cgcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGt < 1:111072/150‑1 (MQ=255) gcAGGTAGATAAAAGAGCCGTTGATAATTACCGTTATGTGAACTTCTACCAGGTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAATTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTg < 2:593325/150‑1 (MQ=255) ggTAGATAAAACAGCCGTTTATACTTACCGTTATCTGAACTTCAAACAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt < 1:374589/150‑1 (MQ=255) gTAGATAAAACAGCCGTTGATACTTACCGTTATCTGACCTTCAACCAGCATTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATg < 2:906735/150‑1 (MQ=255) gTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATg < 1:14364/150‑1 (MQ=255) aTAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAt > 1:1463818/1‑150 (MQ=255) aaaaCAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATTCCCGGTGAATGAGATTc > 2:876053/1‑150 (MQ=255) cAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACAGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCgg > 1:223837/1‑150 (MQ=255) aGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCggg > 2:1488110/1‑150 (MQ=255) aGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCggg < 2:985910/150‑1 (MQ=255) gCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGc > 2:521381/1‑150 (MQ=255) cGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGGCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAt < 1:1304589/150‑1 (MQ=255) tggATACTTACCGTTATCTGAACTTCACCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAATGGCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttg < 1:302483/148‑2 (MQ=255) tGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAtttt > 2:1602064/1‑150 (MQ=255) tGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAtttt < 2:748707/150‑1 (MQ=255) taTACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAttttt < 2:1383372/149‑1 (MQ=255) tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CAGGGCAttttttt > 1:1074934/1‑150 (MQ=255) tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTAttt > 1:964381/1‑150 (MQ=255) aCCGTTATCTGAACTTGAACCAGCTTGCTCAGTACCCCGCGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTATAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGATATTC‑CCGGGCATTTTTTTATTTc < 2:1194605/150‑1 (MQ=255) aCCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc > 1:115805/1‑150 (MQ=255) aCCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc < 2:134238/150‑1 (MQ=255) aCCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTc < 2:466988/150‑1 (MQ=255) ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgtcg < 2:1374425/145‑7 (MQ=38) ccGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGG‑GAATCTCATTCACCGGGCAttgtgtcg > 1:1374425/1‑139 (MQ=38) gTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTaa < 2:1554272/150‑1 (MQ=255) gTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTaa > 2:932825/1‑150 (MQ=255) ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTaaa < 2:459673/150‑1 (MQ=255) aTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGAATTTTTTTATTTCTAAAcc > 2:1258367/1‑150 (MQ=255) aaCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGc < 1:171090/150‑1 (MQ=255) aCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGcc < 2:855937/150‑1 (MQ=255) cTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCg > 1:313670/1‑150 (MQ=255) aaCCAGCTTTCTCAGTACACCGAGAAAGGCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGATGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcc < 2:1183386/150‑1 (MQ=255) aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcc < 2:1249267/150‑1 (MQ=255) ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcctc > 1:638461/1‑148 (MQ=255) ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGc < 1:1472789/150‑1 (MQ=255) ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGAGTTAACGACACTATGAGGGTTGAATGAGGTTA‑CCTGGCATTTTTGTAGTTCTAAAGCATCGCCGTTcggg > 1:31775/1‑147 (MQ=255) cggCTTTCTCGGTGTACTGAGAAAGCTGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCt > 2:713577/3‑150 (MQ=255) aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg > 1:837182/1‑150 (MQ=255) aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg < 2:1006791/150‑1 (MQ=255) aGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg > 1:486243/1‑150 (MQ=255) cTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACAATCGCCGTTCCGCTGtt > 2:310634/1‑150 (MQ=255) ttCTCTGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGTTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt < 2:1031348/150‑1 (MQ=255) ttCTCAGTACACCGAGAAAGCTGAAGGGGCGATTTCCCAGACTGCGGTGTAAAAAGTCAGCGCACGCGCTGCGCATAAACGAAACAATGCCCGGTGAATGAGATTC‑ACGTGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt < 2:906137/150‑1 (MQ=255) ttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt < 2:1380354/150‑1 (MQ=255) ttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCAGTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt > 1:1458694/1‑150 (MQ=255) tcAGTACACAGAGAAAGACGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGACATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtct < 1:758980/150‑1 (MQ=255) cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc > 2:652169/1‑150 (MQ=255) cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtctc > 2:879696/1‑150 (MQ=255) aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc > 2:1626145/1‑150 (MQ=255) aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc > 2:1664294/1‑150 (MQ=255) aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTAAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc < 2:670900/150‑1 (MQ=255) | GAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTC‑CCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCC > NC_000913/134017‑134315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |