Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,444,039 T→G 13.0% L330R (CTG→CGG) ‡ ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,444,0390TG13.0% 245.4 / 25.0 99L330R (CTG→CGG) ‡ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base T (46/40);  new base G (7/6);  total (53/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 104 total aligned reads displayed.

TGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGACA  >  NC_000913/1443890‑1444188
                                                                                                                                                     |                                                                                                                                                     
tgtCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGCCCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCctct                                                                                                                                                       >  2:135756/1‑150 (MQ=255)
    aaaGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCATGGGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAATTTAAGCATGGATAACAGTCAGCTGCCTCTGCAg                                                                                                                                                   >  2:168915/1‑150 (MQ=255)
      aGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCt                                                                                                                                                 <  2:1051749/150‑1 (MQ=255)
       gTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTg                                                                                                                                                <  2:541991/150‑1 (MQ=255)
         cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGAc                                                                                                                                              >  2:925217/1‑150 (MQ=255)
         cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGAc                                                                                                                                              >  2:914113/1‑150 (MQ=255)
         cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCAGAGGCAGCTGAc                                                                                                                                              <  2:419616/150‑1 (MQ=255)
         cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCAGAGGCAGCTGAc                                                                                                                                              <  2:1182167/150‑1 (MQ=255)
         cGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCAGAGGCAGCTGAc                                                                                                                                              <  1:1531050/150‑1 (MQ=255)
          gACAACTGGCAGGCAGGGCTTGAGCACGCTCGGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAATTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGAcc                                                                                                                                             <  1:907574/150‑1 (MQ=255)
          gACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCAGAGGCAGCTGAct                                                                                                                                             <  2:245023/150‑2 (MQ=255)
            cAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACGGGCAGCTGCAGAGGCAGCTGActgt                                                                                                                                           <  2:1392819/150‑4 (MQ=255)
              aCTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTTCTGACCCAGGGGCAAGCGGGTTAGGGAATCGCGGTGCTTAATTTTGGCCAAGGAAAATTAAGCATGGATAACAGTCTGCTGCCTCTGCAGCGGACCGGTg                                                                                                                                         >  1:702909/1‑150 (MQ=255)
               cTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGa                                                                                                                                        <  2:1651625/150‑1 (MQ=255)
                 ggcaggcaGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAg                                                                                                                                      >  1:1300083/1‑150 (MQ=255)
                 ggcaggcaGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAg                                                                                                                                      >  2:1068194/1‑150 (MQ=255)
                 ggcaggcaGGGCTCGAGCACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAg                                                                                                                                      <  1:1532487/150‑1 (MQ=255)
                  gcaggcagGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGc                                                                                                                                     >  1:1030697/1‑150 (MQ=255)
                   caggcaggGCTTGAGAACGCTCTGGTCAGCGGACGCCTGAGTGTGCTGACCCAGGGGCAAGCGGGTATGGGCAACGCGGTGCTTAATTTTGGCACAGGAAAATTAGGCATGGATAACCGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCg                                                                                                                                    >  1:263158/1‑150 (MQ=255)
                     ggcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                                                                                                                  <  1:436338/150‑1 (MQ=255)
                          ggCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGGGAAGCGATACAgg                                                                                                                             >  2:324413/1‑150 (MQ=255)
                          ggCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAAGTAAGCATGGTTTACAGTCAGCTGCCTCTGCCGCTGCCCGGTGAAGCGAAACAgg                                                                                                                             >  1:130731/1‑150 (MQ=255)
                                 gaACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGCCCt                                                                                                                      >  1:1317575/1‑150 (MQ=255)
                                 caaCGCTCGGGTCAGTGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGTGCAACGCGGTGCTTAATTTTGGCCCAGTAAAATTAAGCATGGATAACAGTCAGCTGCCGCTGCAGCTGACCGGTGAAGCGAAACAGTCGGACCt                                                                                                                      <  1:836539/149‑1 (MQ=255)
                                     gCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCAtt                                                                                                                  >  2:764454/1‑150 (MQ=255)
                                      ctctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCAttt                                                                                                                 >  2:605872/1‑150 (MQ=255)
                                      ctctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGAGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCAttt                                                                                                                 <  2:1625621/150‑1 (MQ=255)
                                       tctGGTCAGCGGAAGACTGATTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTTCCTCTGGAGCTGACCGGTGAAGCGATACAGGCGGACCTCAtttt                                                                                                                <  1:169884/150‑1 (MQ=255)
                                         tGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTtat                                                                                                              <  1:460523/150‑1 (MQ=255)
                                          ggTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTtata                                                                                                             >  1:1423186/1‑150 (MQ=255)
                                          ggTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTtata                                                                                                             >  1:533440/1‑150 (MQ=255)
                                          ggTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTta                                                                                                               <  1:1403623/148‑1 (MQ=255)
                                          ggTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTta                                                                                                               >  2:1403623/1‑148 (MQ=255)
                                             cAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGc                                                                                                          >  1:966135/1‑150 (MQ=255)
                                              aGCGGACGACTGAGTGTGCTGACCCATGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGcc                                                                                                         <  1:914113/150‑1 (MQ=255)
                                                 ggACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGTCGGACCTCATTTTATATGCCCGt                                                                                                      >  2:94222/1‑150 (MQ=255)
                                                 ggACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGt                                                                                                      >  2:382773/1‑150 (MQ=255)
                                                 ggACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGt                                                                                                      >  2:335632/1‑150 (MQ=255)
                                                     gacTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTAc                                                                                                  <  1:206519/150‑1 (MQ=255)
                                                      acTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTAcc                                                                                                 <  1:1245389/150‑1 (MQ=255)
                                                      acTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTATGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTAcc                                                                                                 >  1:502108/1‑150 (MQ=255)
                                                           gtgtGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgcgc                                                                                            <  1:1685137/150‑1 (MQ=255)
                                                           gtgtGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgcgc                                                                                            <  1:61085/150‑1 (MQ=255)
                                                             gtgCTGACCGAGGGGCAAGCTGGTAAGGGCATCGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCATCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAg                                                                                          <  1:1127129/150‑1 (MQ=255)
                                                             gtgCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAg                                                                                          >  1:148778/1‑150 (MQ=255)
                                                             gtgCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAg                                                                                          <  1:75275/150‑1 (MQ=255)
                                                                cTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGCCCGGTGCAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTa                                                                                       >  2:1117731/1‑150 (MQ=255)
                                                                    cccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTTCGCAGCTAAGTg                                                                                   >  2:1112717/1‑150 (MQ=255)
                                                                    cccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTg                                                                                   >  2:1458628/1‑150 (MQ=255)
                                                                     ccAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTgg                                                                                  >  1:623741/1‑150 (MQ=255)
                                                                      cAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGa                                                                                 <  1:785387/150‑1 (MQ=255)
                                                                       aGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGaa                                                                                >  2:1302317/1‑150 (MQ=255)
                                                                        ggggCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAg                                                                               >  2:1013530/1‑150 (MQ=255)
                                                                            cAAGCGGGTAAGGGCAACGCGGTGATTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGACCGATTACCTGCGCAGCTAAGTGGAAgtctg                                                                           >  1:61613/1‑150 (MQ=255)
                                                                              aGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgtctgtc                                                                         >  2:1211678/1‑150 (MQ=255)
                                                                               gCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAgtctgtct                                                                        <  1:166072/150‑1 (MQ=255)
                                                                                    tAAGGGCAACGCGGGGCTTAATTTTGTACCAGGATAATTAAGCATGGTTAACAGTCAGCTGCCGCTGCAGCTGACCGGTGATGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGGCTGAccc                                                                   <  1:900192/150‑1 (MQ=255)
                                                                                      aGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTCACCCaa                                                                 >  1:113804/1‑150 (MQ=255)
                                                                                           aaCGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTg                                                            <  2:1407467/150‑1 (MQ=255)
                                                                                              gcgGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGAcc                                                         <  1:1181770/150‑1 (MQ=255)
                                                                                              gcgGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGAcc                                                         <  2:418439/150‑1 (MQ=255)
                                                                                                ggTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCtt                                                       <  2:1030697/150‑1 (MQ=255)
                                                                                                 gTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCttt                                                      >  2:638729/1‑150 (MQ=255)
                                                                                                   gCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGa                                                    >  1:318851/1‑150 (MQ=255)
                                                                                                    cTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAg                                                   <  2:318851/150‑1 (MQ=255)
                                                                                                    attAATTTTGGCCCAGTAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCACTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAg                                                   <  2:1356526/149‑1 (MQ=255)
                                                                                                     ttAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGc                                                  <  1:1056630/150‑1 (MQ=255)
                                                                                                     ttAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGc                                                  <  1:546521/150‑1 (MQ=255)
                                                                                                           ttGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGgcg                                            <  1:925217/150‑1 (MQ=255)
                                                                                                                ccAGGAAAATTAAGCATGGATAACAGACAGATGCCTCGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTCTCTGACCCAACGCTGACCTTTGAGACAGGCGCGTTa                                       >  1:909159/1‑150 (MQ=255)
                                                                                                                    gAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGCAACAGGCGGACCTCATATTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGCCCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTc                                   >  1:251951/1‑150 (MQ=255)
                                                                                                                     aaacTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATAGTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCg                                  <  1:168915/150‑1 (MQ=255)
                                                                                                                       aaTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGtt                                >  2:1101989/1‑150 (MQ=255)
                                                                                                                       aaTTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGtt                                >  1:1432526/1‑150 (MQ=255)
                                                                                                                         ttAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGACGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCa                              >  1:1033800/1‑150 (MQ=255)
                                                                                                                            aGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAg                           <  1:519958/150‑1 (MQ=255)
                                                                                                                            aGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAg                           <  2:1423186/150‑1 (MQ=255)
                                                                                                                            aGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAg                           >  2:347801/1‑150 (MQ=255)
                                                                                                                              cATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTCACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAggg                         >  1:1349941/1‑150 (MQ=255)
                                                                                                                               aTGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGa                        <  1:1068194/150‑1 (MQ=255)
                                                                                                                                tGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACACAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGaa                       >  1:107943/1‑150 (MQ=255)
                                                                                                                                 ggATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAag                      >  1:458197/1‑150 (MQ=255)
                                                                                                                                   tcaccggTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCGCAGTGTATCTGCCCGTTTACAGGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAagag                    >  1:254689/7‑150 (MQ=255)
                                                                                                                                       ctgTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGAAGCTAAGTGGAAGTCTGTCTGACCCAACGATGAGCTTTGAGCCAGGCTCGTTAATTGGTTCAAAGGGAAGAGTCAt                >  2:825589/3‑150 (MQ=255)
                                                                                                                                       cggTCAGCTGCAGAGGCGGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCAt                >  1:1717479/3‑150 (MQ=255)
                                                                                                                                       cggTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCaa                >  1:138030/3‑149 (MQ=255)
                                                                                                                                       cggTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCAt                >  1:462698/3‑150 (MQ=255)
                                                                                                                                       cggTCAGCTGCAGAGGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGGTACTTCGTTCAAAGGGAAGAGTCAt                >  1:1160101/3‑150 (MQ=255)
                                                                                                                                       cAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCAt                >  1:1643364/1‑150 (MQ=255)
                                                                                                                                        aGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATc               >  2:234019/1‑150 (MQ=255)
                                                                                                                                         gTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCg              <  2:1540440/150‑1 (MQ=255)
                                                                                                                                            aGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGAtt           >  1:767299/1‑150 (MQ=255)
                                                                                                                                               tGCCTCTGCAGCTGCCCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCGCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGCCCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTAGGTCAAAGGGTAGAGTCATCGATTCGc        >  2:152934/1‑150 (MQ=255)
                                                                                                                                                gCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCt       <  2:502108/150‑1 (MQ=255)
                                                                                                                                                 ccTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTg      <  1:335632/150‑1 (MQ=255)
                                                                                                                                                 ccTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTg      <  1:94222/150‑1 (MQ=255)
                                                                                                                                                  ctgtGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTAATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTgg     <  2:799246/147‑1 (MQ=255)
                                                                                                                                                  ctctGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTTGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGATTCATCGATTCGCTgg     <  1:983597/150‑1 (MQ=255)
                                                                                                                                                     tGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGACa  >  1:1011811/1‑150 (MQ=255)
                                                                                                                                                     tGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGACa  >  1:1005294/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGCGCAGCTAAGTGGAAGTCTGTCTGACCCAACGCTGACCTTTGAGCCAGGCGCGTTACTTCGTTCAAAGGGAAGAGTCATCGATTCGCTGGACA  >  NC_000913/1443890‑1444188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: