Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,445,616 G→C 6.0% A856P (GCA→CCA)  ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,445,6160GC 6.0% 360.3 / 9.5 113A856P (GCA→CCA) ydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (58/48);  new base C (2/4);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 114 total aligned reads displayed.

AGATAATCTCGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCT  >  NC_000913/1445468‑1445764
                                                                                                                                                    |                                                                                                                                                    
aGATAATCTCGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGc                                                                                                                                                     >  2:1643651/1‑150 (MQ=255)
       ctcGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTg                                                                                                                                              <  1:1701988/150‑1 (MQ=255)
         cGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCAAGGCATGGCTCGa                                                                                                                                            >  1:226123/1‑150 (MQ=255)
            tttATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                         <  1:996583/150‑3 (MQ=255)
            tttATTAACCATGCAGGCCAAAATTACATGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                         <  1:1159354/150‑3 (MQ=255)
            tttAATAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCa                                                                                                                                         <  1:1123545/150‑1 (MQ=255)
            ttaattaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                         <  1:1052714/147‑3 (MQ=255)
             ttattaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATAATGAAGAGTATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAg                                                                                                                                        >  1:1419488/1‑150 (MQ=255)
               attaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGaa                                                                                                                                      >  1:833341/1‑150 (MQ=255)
               attaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCCGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTATTACGCTGTGGCGCAGTTTACCCTTTGGCGATAATCTCAAGGCATGGCTGGAGCAGaa                                                                                                                                      >  2:784680/1‑150 (MQ=255)
                ttaACCATGCAGTCAAACATTACAGTTACCAGTCGAGTTGATGTTAAAAGCGGTACGGTAAACCTTAATTACCATGATGAAGAGAATATTTTTACGCTGGGGCGCAGTTTACGCTTTGGCGATAATCTCCAGACATGCCTGTAgattatc                                                                                                                                     <  2:805916/150‑7 (MQ=255)
                 taagcctGCAGGCCAACATTACAGGTACCTGTCGCGTTGATGGTAAAAGCGGTACGGTAAAACTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACg                                                                                                                                    <  2:340314/144‑1 (MQ=255)
                 taACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcg                                                                                                                                    <  1:946801/150‑8 (MQ=255)
                  aaCCATGCAGGCCAACATTACAGTTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                   <  2:369014/150‑1 (MQ=255)
                  aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCATGCATGGCTGGAGCAGAACGc                                                                                                                                   >  1:177739/1‑150 (MQ=255)
                  aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                   >  1:23968/1‑150 (MQ=255)
                  aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCGTTGGCGATAATCTCCAGGCATGGCTGGAGCATACCGc                                                                                                                                   >  1:1543742/1‑150 (MQ=255)
                  aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGCAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                   >  2:1398976/1‑150 (MQ=255)
                   aCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCa                                                                                                                                  >  1:1180798/1‑150 (MQ=255)
                       tGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACATTAATTACCATAATGAATAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCAAGGCATGGATCGAGCAGAACGCACGta                                                                                                                              >  2:929172/1‑149 (MQ=255)
                         cAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGAGGTACGGTAAACCTTAATTACCATCAAGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTATGGCGATAATCTCCATGCATGGATGGAGCAGAACGCACGTCTg                                                                                                                            >  1:280875/1‑150 (MQ=255)
                            gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGTGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                         <  2:1575303/150‑1 (MQ=255)
                            gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                         <  1:1716599/150‑1 (MQ=255)
                            gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                         >  1:204515/1‑150 (MQ=255)
                             ccAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCgg                                                                                                                        >  2:679983/1‑150 (MQ=255)
                                  aTTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTCTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAAt                                                                                                                   >  2:553194/1‑150 (MQ=255)
                                    tACAGGTACCAGTCGCGTTGAGGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGCGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCCGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGa                                                                                                                 <  2:76226/150‑1 (MQ=255)
                                    tACAGGGACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGa                                                                                                                 <  2:741540/150‑1 (MQ=255)
                                     aCAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTATGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                                                                <  1:14069/150‑1 (MQ=255)
                                            ccAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGc                                                                                                         <  1:425647/150‑1 (MQ=255)
                                              aGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCaa                                                                                                       <  1:659642/150‑1 (MQ=255)
                                               gTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAg                                                                                                      >  2:902220/1‑150 (MQ=255)
                                                  gcgTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGaa                                                                                                   >  1:845968/1‑150 (MQ=255)
                                                   cgTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGaaa                                                                                                  >  1:1193405/1‑150 (MQ=255)
                                                     ttGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAag                                                                                                <  1:212444/150‑1 (MQ=255)
                                                     ttGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGAGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAag                                                                                                <  2:188054/150‑1 (MQ=255)
                                                         tGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTACCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                                                            >  1:911773/1‑150 (MQ=255)
                                                          ggTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtgt                                                                                           <  2:425665/150‑1 (MQ=255)
                                                           gTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGtgtg                                                                                          >  1:1318825/1‑150 (MQ=255)
                                                             aaaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAg                                                                                        <  1:661119/150‑1 (MQ=255)
                                                              aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                                                                       >  2:1727281/1‑150 (MQ=255)
                                                              aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                                                                       <  2:1180798/150‑1 (MQ=255)
                                                               aaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGa                                                                                      >  1:541641/1‑150 (MQ=255)
                                                                 gCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTAGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCAGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaa                                                                                    >  1:1627006/1‑150 (MQ=255)
                                                                   ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGTCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                  >  1:117282/1‑150 (MQ=255)
                                                                   ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                  <  2:691570/150‑1 (MQ=255)
                                                                   ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                  >  1:402125/1‑150 (MQ=255)
                                                                    gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAAc                                                                                 >  2:1554774/1‑150 (MQ=255)
                                                                     tacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGTAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACa                                                                                <  2:1569455/150‑1 (MQ=255)
                                                                      acggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCATGGAAAAGAGTGTGAGGAAAAACaa                                                                               <  1:507590/150‑1 (MQ=255)
                                                                         gtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGa                                                                            >  2:461743/1‑150 (MQ=255)
                                                                         gtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGa                                                                            >  2:51701/1‑150 (MQ=255)
                                                                           aaaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaaa                                                                          >  1:1077307/1‑150 (MQ=255)
                                                                           aaaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGGTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGGGTGAGGAAAAACAATGaaa                                                                          <  2:931516/150‑1 (MQ=255)
                                                                            aaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaaaa                                                                         >  2:1320559/1‑150 (MQ=255)
                                                                               cTTAATTACCATCAAGAAGAGAATATTTTTACGCTGAGGCGAAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACTCACGTCTGCCGTGAAATGACTTTCCGCAAGGAAAAGAGTGTCAGGAAAAACAATGAAAAttt                                                                      >  1:276994/1‑150 (MQ=255)
                                                                                  aaTTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTAc                                                                   <  1:901381/150‑1 (MQ=255)
                                                                                       ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCt                                                              >  1:397135/1‑150 (MQ=255)
                                                                                        cagcatGAAGAGAATATTTTTACGCTGTGGCGCTGTGTACGCTTAGGCGATAATCTACAGGCATGGCTGGAGCAGAACGCTCGTCTGCCGGGAAATGACTGTACGCAAGGAAATGAGTGTGAGGAAAAACAATGAAAATTATACTGGATg                                                             >  1:137204/4‑150 (MQ=255)
                                                                                           catGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGt                                                          <  1:679983/150‑1 (MQ=255)
                                                                                            atGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt                                                         >  1:724860/1‑150 (MQ=255)
                                                                                             tGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGATCGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg                                                        <  1:397223/150‑1 (MQ=255)
                                                                                               aaGAGTATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGACTGGAGCTGAACTCACGTCTGCAGGGAAATGACTGTCCGCAAGCAAAAGAGTGTGATAAAAAACAATGATTATTTTACTGGCTGCGTTGAc                                                      >  1:264366/1‑150 (MQ=255)
                                                                                               aaGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc                                                      >  1:588952/1‑150 (MQ=255)
                                                                                               aaGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc                                                      >  2:1714622/1‑150 (MQ=255)
                                                                                                agagAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg                                                     <  2:944141/150‑1 (MQ=255)
                                                                                                agagAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg                                                     >  2:1190456/1‑150 (MQ=255)
                                                                                                   gaATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCa                                                  >  2:473391/1‑150 (MQ=255)
                                                                                                    aaTATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCAt                                                 >  2:1298413/1‑150 (MQ=255)
                                                                                                       atTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCtt                                              >  1:562914/1‑150 (MQ=255)
                                                                                                           ttccGCTGTGGCGCAGTTTACGCTTTGGCGATACTCTCCAGGCATGTCTGGAGCAGAACGCACGTCTGCCGGGAAATTACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTAt                                          <  2:276082/147‑1 (MQ=255)
                                                                                                            tACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATg                                         >  2:278132/1‑150 (MQ=255)
                                                                                                             actcTGTTGCGAAGTTTACGCTTTGGCGATAATCTCAAGGCATTGCTGGAGCTGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGACGAAAAACTATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGc                                        <  1:929172/147‑1 (MQ=255)
                                                                                                              cGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCt                                       <  2:833341/150‑1 (MQ=255)
                                                                                                                 tgtgGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGGCATCTTTTATGCTGGt                                    <  1:293602/150‑1 (MQ=255)
                                                                                                                         aGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTa                            <  2:1532848/150‑1 (MQ=255)
                                                                                                                            ttACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGc                         <  1:698206/150‑1 (MQ=255)
                                                                                                                                 cTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGc                    >  2:401586/1‑150 (MQ=255)
                                                                                                                                  tttGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCa                   >  1:745397/1‑150 (MQ=255)
                                                                                                                                    tGGCGATAATCTCCAGGCATGGCTGGAGCAGCACGCTCGTCTGCCGGGAAATGACTGGCCGCAAGGAAAAGAGTGTGCGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCAtt                 >  1:1381070/1‑150 (MQ=255)
                                                                                                                                    tGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCGTTTATGCTGGTTGGCTGTCCGCCTCGCAtt                 >  1:1053263/1‑150 (MQ=255)
                                                                                                                                    tGGCCATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACAGGCTGCGTTGACGTCATCTTTTATGATGGTTGGCTGTACGCCTCGCAtt                 >  1:1435941/1‑150 (MQ=255)
                                                                                                                                     ggCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTg                >  1:518284/1‑150 (MQ=255)
                                                                                                                                      gCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGa               <  1:830365/150‑1 (MQ=255)
                                                                                                                                        gATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAg             <  2:397135/150‑1 (MQ=255)
                                                                                                                                        gATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAg             <  1:431579/150‑1 (MQ=255)
                                                                                                                                           ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGCCGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCg          >  1:1226829/4‑150 (MQ=255)
                                                                                                                                           ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCg          >  1:353481/4‑150 (MQ=255)
                                                                                                                                            aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGc         <  1:998436/150‑1 (MQ=255)
                                                                                                                                            aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGc         >  2:1347643/1‑150 (MQ=255)
                                                                                                                                            aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCAGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCTTTGAAGTCGc         >  1:254314/1‑150 (MQ=255)
                                                                                                                                             tctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCt        <  2:204515/150‑1 (MQ=255)
                                                                                                                                              ctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTg       >  1:466212/1‑150 (MQ=255)
                                                                                                                                               tcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAACAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGAGTTGACGTCATCTTATATGCTGGTTGGCTGTACGCCTGGCATTGAAGTCGcgtc      >  2:67888/1‑147 (MQ=255)
                                                                                                                                                ccAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTTCGTTGACGTCATCTTTTATGCTGGTTGGCGGTACGACTCGCATTGAAGTCGCTGCa     >  2:108171/1‑150 (MQ=255)
                                                                                                                                                ccAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCa     >  2:281763/1‑150 (MQ=255)
                                                                                                                                                  aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAATACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCAcc   <  1:691497/150‑1 (MQ=255)
                                                                                                                                                  aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCAcc   <  2:918218/150‑1 (MQ=255)
                                                                                                                                                  aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCAcc   <  2:1419774/150‑1 (MQ=255)
                                                                                                                                                   ggCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCt  <  2:902101/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGATAATCTCGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCT  >  NC_000913/1445468‑1445764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: