Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,445,621 G→C 6.7% W857C (TGG→TGC ydbH → putative membrane‑anchored protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,445,6210GC 6.7% 356.2 / 11.0 114W857C (TGG→TGCydbHputative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base G (56/48);  new base C (2/5);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 114 total aligned reads displayed.

CTCGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCTAAGG  >  NC_000913/1445475‑1445768
                                                                                                                                                  |                                                                                                                                                   
ctcGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTg                                                                                                                                                  <  1:1701988/150‑1 (MQ=255)
  cGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCAAGGCATGGCTCGa                                                                                                                                                >  1:226123/1‑150 (MQ=255)
   ggTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAg                                                                                                                                               <  1:678134/150‑1 (MQ=255)
     tttATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                             <  1:996583/150‑3 (MQ=255)
     tttATTAACCATGCAGGCCAAAATTACATGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                             <  1:1159354/150‑3 (MQ=255)
     tttAATAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCa                                                                                                                                             <  1:1123545/150‑1 (MQ=255)
     ttaattaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgat                                                                                                                                             <  1:1052714/147‑3 (MQ=255)
      ttattaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATAATGAAGAGTATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAg                                                                                                                                            >  1:1419488/1‑150 (MQ=255)
        attaACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCCGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTATTACGCTGTGGCGCAGTTTACCCTTTGGCGATAATCTCAAGGCATGGCTGGAGCAGaa                                                                                                                                          >  2:784680/1‑150 (MQ=255)
         ttaACCATGCAGTCAAACATTACAGTTACCAGTCGAGTTGATGTTAAAAGCGGTACGGTAAACCTTAATTACCATGATGAAGAGAATATTTTTACGCTGGGGCGCAGTTTACGCTTTGGCGATAATCTCCAGACATGCCTGTAgattatc                                                                                                                                         <  2:805916/150‑7 (MQ=255)
          taagcctGCAGGCCAACATTACAGGTACCTGTCGCGTTGATGGTAAAAGCGGTACGGTAAAACTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACg                                                                                                                                        <  2:340314/144‑1 (MQ=255)
          taACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGCCATGCCTGGAgattatcg                                                                                                                                        <  1:946801/150‑8 (MQ=255)
           aaCCATGCAGGCCAACATTACAGTTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                       <  2:369014/150‑1 (MQ=255)
           aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCATGCATGGCTGGAGCAGAACGc                                                                                                                                       >  1:177739/1‑150 (MQ=255)
           aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                       >  1:23968/1‑150 (MQ=255)
           aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCGTTGGCGATAATCTCCAGGCATGGCTGGAGCATACCGc                                                                                                                                       >  1:1543742/1‑150 (MQ=255)
           aaCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGCAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGc                                                                                                                                       >  2:1398976/1‑150 (MQ=255)
            aCCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCa                                                                                                                                      >  1:1180798/1‑150 (MQ=255)
                tGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACATTAATTACCATAATGAATAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCAAGGCATGGATCGAGCAGAACGCACGta                                                                                                                                  >  2:929172/1‑149 (MQ=255)
                  cAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGAGGTACGGTAAACCTTAATTACCATCAAGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTATGGCGATAATCTCCATGCATGGATGGAGCAGAACGCACGTCTg                                                                                                                                >  1:280875/1‑150 (MQ=255)
                     gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGTGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                             <  2:1575303/150‑1 (MQ=255)
                     gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                             <  1:1716599/150‑1 (MQ=255)
                     gCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCg                                                                                                                             >  1:204515/1‑150 (MQ=255)
                      ccAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCgg                                                                                                                            >  2:679983/1‑150 (MQ=255)
                       cAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCggg                                                                                                                           <  1:841507/150‑1 (MQ=255)
                           aTTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTCTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAAt                                                                                                                       >  2:553194/1‑150 (MQ=255)
                             tACAGGTACCAGTCGCGTTGAGGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGCGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCCGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGa                                                                                                                     <  2:76226/150‑1 (MQ=255)
                             tACAGGGACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGa                                                                                                                     <  2:741540/150‑1 (MQ=255)
                              aCAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTATGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGAc                                                                                                                    <  1:14069/150‑1 (MQ=255)
                               cAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACt                                                                                                                   >  2:1175794/1‑150 (MQ=255)
                                     ccAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGc                                                                                                             <  1:425647/150‑1 (MQ=255)
                                       aGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCaa                                                                                                           <  1:659642/150‑1 (MQ=255)
                                        gTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAg                                                                                                          >  2:902220/1‑150 (MQ=255)
                                           gcgTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGaa                                                                                                       >  1:845968/1‑150 (MQ=255)
                                            cgTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGaaa                                                                                                      >  1:1193405/1‑150 (MQ=255)
                                              ttGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAag                                                                                                    <  2:1490873/150‑1 (MQ=255)
                                              ttGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAag                                                                                                    <  1:212444/150‑1 (MQ=255)
                                              ttGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGAGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAag                                                                                                    <  2:188054/150‑1 (MQ=255)
                                                  tGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTACCGGGAAATGACTGTCCGCAAGGAAAAGAgtg                                                                                                >  1:911773/1‑150 (MQ=255)
                                                   ggTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAgtgt                                                                                               <  2:425665/150‑1 (MQ=255)
                                                    gTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGtgtg                                                                                              >  1:1318825/1‑150 (MQ=255)
                                                       aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                                                                           <  2:1180798/150‑1 (MQ=255)
                                                       aaaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAgg                                                                                           >  2:1727281/1‑150 (MQ=255)
                                                        aaGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGa                                                                                          >  1:541641/1‑150 (MQ=255)
                                                          gCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTAGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCAGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaa                                                                                        >  1:1627006/1‑150 (MQ=255)
                                                            ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGTCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                      >  1:117282/1‑150 (MQ=255)
                                                            ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                      >  1:402125/1‑150 (MQ=255)
                                                            ggtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGaaaaa                                                                                      <  2:691570/150‑1 (MQ=255)
                                                             gtacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAAc                                                                                     >  2:1554774/1‑150 (MQ=255)
                                                              tacggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGTAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACa                                                                                    <  2:1569455/150‑1 (MQ=255)
                                                               acggtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCATGGAAAAGAGTGTGAGGAAAAACaa                                                                                   <  1:507590/150‑1 (MQ=255)
                                                                  gtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGa                                                                                >  2:51701/1‑150 (MQ=255)
                                                                  gtaAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGa                                                                                >  2:461743/1‑150 (MQ=255)
                                                                    aaaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaaa                                                                              >  1:1077307/1‑150 (MQ=255)
                                                                    aaaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGGTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGGGTGAGGAAAAACAATGaaa                                                                              <  2:931516/150‑1 (MQ=255)
                                                                     aaCCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGaaaa                                                                             >  2:1320559/1‑150 (MQ=255)
                                                                        cTTAATTACCATCAAGAAGAGAATATTTTTACGCTGAGGCGAAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACTCACGTCTGCCGTGAAATGACTTTCCGCAAGGAAAAGAGTGTCAGGAAAAACAATGAAAAttt                                                                          >  1:276994/1‑150 (MQ=255)
                                                                           aaTTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTAc                                                                       <  1:901381/150‑1 (MQ=255)
                                                                                ccATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCt                                                                  >  1:397135/1‑150 (MQ=255)
                                                                                 cagcatGAAGAGAATATTTTTACGCTGTGGCGCTGTGTACGCTTAGGCGATAATCTACAGGCATGGCTGGAGCAGAACGCTCGTCTGCCGGGAAATGACTGTACGCAAGGAAATGAGTGTGAGGAAAAACAATGAAAATTATACTGGATg                                                                 >  1:137204/4‑150 (MQ=255)
                                                                                    catGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGt                                                              <  1:679983/150‑1 (MQ=255)
                                                                                     atGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGtt                                                             >  1:724860/1‑150 (MQ=255)
                                                                                      tGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGATCGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTg                                                            <  1:397223/150‑1 (MQ=255)
                                                                                        aaGAGTATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGACTGGAGCTGAACTCACGTCTGCAGGGAAATGACTGTCCGCAAGCAAAAGAGTGTGATAAAAAACAATGATTATTTTACTGGCTGCGTTGAc                                                          >  1:264366/1‑150 (MQ=255)
                                                                                        aaGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc                                                          >  1:588952/1‑150 (MQ=255)
                                                                                        aaGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGAc                                                          >  2:1714622/1‑150 (MQ=255)
                                                                                         agagAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg                                                         <  2:944141/150‑1 (MQ=255)
                                                                                         agagAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACg                                                         >  2:1190456/1‑150 (MQ=255)
                                                                                            gaATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCa                                                      >  2:473391/1‑150 (MQ=255)
                                                                                             aaTATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCAt                                                     >  2:1298413/1‑150 (MQ=255)
                                                                                                atTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCtt                                                  >  1:562914/1‑150 (MQ=255)
                                                                                                    ttccGCTGTGGCGCAGTTTACGCTTTGGCGATACTCTCCAGGCATGTCTGGAGCAGAACGCACGTCTGCCGGGAAATTACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTAt                                              <  2:276082/147‑1 (MQ=255)
                                                                                                     tACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATg                                             >  2:278132/1‑150 (MQ=255)
                                                                                                      actcTGTTGCGAAGTTTACGCTTTGGCGATAATCTCAAGGCATTGCTGGAGCTGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGACGAAAAACTATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGc                                            <  1:929172/147‑1 (MQ=255)
                                                                                                       cGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCt                                           <  2:833341/150‑1 (MQ=255)
                                                                                                          tgtgGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGGCATCTTTTATGCTGGt                                        <  1:293602/150‑1 (MQ=255)
                                                                                                                  aGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTa                                <  2:1532848/150‑1 (MQ=255)
                                                                                                                     ttACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGc                             <  1:698206/150‑1 (MQ=255)
                                                                                                                          cTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGc                        >  2:401586/1‑150 (MQ=255)
                                                                                                                           tttGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCa                       >  1:745397/1‑150 (MQ=255)
                                                                                                                             tGGCGATAATCTCCAGGCATGGCTGGAGCAGCACGCTCGTCTGCCGGGAAATGACTGGCCGCAAGGAAAAGAGTGTGCGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCAtt                     >  1:1381070/1‑150 (MQ=255)
                                                                                                                             tGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCGTTTATGCTGGTTGGCTGTCCGCCTCGCAtt                     >  1:1053263/1‑150 (MQ=255)
                                                                                                                             tGGCCATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACAGGCTGCGTTGACGTCATCTTTTATGATGGTTGGCTGTACGCCTCGCAtt                     >  1:1435941/1‑150 (MQ=255)
                                                                                                                              ggCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTg                    >  1:518284/1‑150 (MQ=255)
                                                                                                                               gCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGa                   <  1:830365/150‑1 (MQ=255)
                                                                                                                                 gATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAg                 <  2:397135/150‑1 (MQ=255)
                                                                                                                                 gATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAg                 <  1:431579/150‑1 (MQ=255)
                                                                                                                                    ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGCCGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCg              >  1:1226829/4‑150 (MQ=255)
                                                                                                                                    ctgctcCAGCCATGCCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCg              >  1:353481/4‑150 (MQ=255)
                                                                                                                                     aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGc             >  2:1347643/1‑150 (MQ=255)
                                                                                                                                     aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGc             <  1:998436/150‑1 (MQ=255)
                                                                                                                                     aTCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCAGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCTTTGAAGTCGc             >  1:254314/1‑150 (MQ=255)
                                                                                                                                      tctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCt            <  2:204515/150‑1 (MQ=255)
                                                                                                                                       ctcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTg           >  1:466212/1‑150 (MQ=255)
                                                                                                                                        tcCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAACAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGAGTTGACGTCATCTTATATGCTGGTTGGCTGTACGCCTGGCATTGAAGTCGcgtc          >  2:67888/1‑147 (MQ=255)
                                                                                                                                         ccAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCa         >  2:281763/1‑150 (MQ=255)
                                                                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAATACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCAcc       <  1:691497/150‑1 (MQ=255)
                                                                                                                                           aGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCAcc       <  2:918218/150‑1 (MQ=255)
                                                                                                                                            ggCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCt      <  2:902101/150‑1 (MQ=255)
                                                                                                                                                tGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCTAAgg  >  1:635743/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCGGTTTATTAACCATGCAGGCCAACATTACAGGTACCAGTCGCGTTGATGGTAAAAGCGGTACGGTAAACCTTAATTACCATCATGAAGAGAATATTTTTACGCTGTGGCGCAGTTTACGCTTTGGCGATAATCTCCAGGCATGGCTGGAGCAGAACGCACGTCTGCCGGGAAATGACTGTCCGCAAGGAAAAGAGTGTGAGGAAAAACAATGAAAATTTTACTGGCTGCGTTGACGTCATCTTTTATGCTGGTTGGCTGTACGCCTCGCATTGAAGTCGCTGCACCTAAGG  >  NC_000913/1445475‑1445768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: