Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,449,807 C→G 9.2% G515R (GGT→CGT)  tynA ← tyramine oxidase, copper‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,449,8070CG 9.2% 307.5 / 18.2 107G515R (GGT→CGT) tynAtyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand):  ref base C (43/54);  new base G (8/2);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 4.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

Only 100 of 110 total aligned reads displayed.

CAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC  >  NC_000913/1449658‑1449955
                                                                                                                                                     |                                                                                                                                                    
cAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAcc                                                                                                                                                      >  1:1479702/1‑150 (MQ=255)
cAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCAcc                                                                                                                                                      >  1:1221236/1‑150 (MQ=255)
 aGATCGATGCGGAAATTATTAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCTTGGCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTTACCGATTCGATGCGCGTAGCACCg                                                                                                                                                     <  2:329031/150‑1 (MQ=255)
      gAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATc                                                                                                                                                <  1:233124/150‑1 (MQ=255)
        ggCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGa                                                                                                                                              >  1:40344/1‑150 (MQ=255)
        ggCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTCCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGa                                                                                                                                              >  1:772315/1‑150 (MQ=255)
         gCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCGGTGCTACGGGCATCGaa                                                                                                                                             <  1:1121696/150‑2 (MQ=255)
          cGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGATTCGATGCCCGTAGCACCGGCATCGATg                                                                                                                                            <  1:753783/150‑1 (MQ=255)
                     aaTATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGcc                                                                                                                                 >  1:856341/1‑150 (MQ=255)
                         tGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTATAGCTTTAACACATTTCACCGCTTCGATGCACGTAGCACCGGCATCGATGCCAATAGTGACGttt                                                                                                                             >  1:394341/1‑150 (MQ=255)
                                      taCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATcc                                                                                                                <  1:1440970/150‑1 (MQ=255)
                                       aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                               >  2:388749/1‑150 (MQ=255)
                                       aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                               >  2:1049898/1‑150 (MQ=255)
                                       aCCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCa                                                                                                               >  2:275449/1‑150 (MQ=255)
                                         gcACGATTTTGTGATCGTTAAGCGTGCCGTAGCGCGTGTGATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTATCACCTTTCACCGCTTGGATGCCCGTTGCACCGGCATCGATGCGAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                             <  1:517035/149‑1 (MQ=255)
                                         ccACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCTGCATCGAAGCCAATAGTGCCGTTTTCATGGAAGATCCAGt                                                                                                             >  2:72275/1‑150 (MQ=255)
                                          cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                            >  1:759493/1‑150 (MQ=255)
                                          cACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTc                                                                                                            >  2:1123358/1‑150 (MQ=255)
                                             gATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCgaa                                                                                                         <  1:769638/150‑1 (MQ=255)
                                              atatTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCaaaa                                                                                                        <  1:1642353/150‑1 (MQ=255)
                                               tatTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCaaaaa                                                                                                       <  1:184635/150‑1 (MQ=255)
                                                 ttGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATg                                                                                                     <  2:735915/150‑1 (MQ=255)
                                                    tgATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAg                                                                                                  <  2:45828/150‑1 (MQ=255)
                                                     gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                 <  1:1540775/150‑1 (MQ=255)
                                                     gATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCAGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGt                                                                                                 <  2:186537/150‑1 (MQ=255)
                                                         gATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAATTGTAGTCATa                                                                                             >  2:887112/1‑150 (MQ=255)
                                                         gATAAGAGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGGTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGATGATCCAGTCAAAAATGTAGTCATa                                                                                             >  2:917381/1‑150 (MQ=255)
                                                          atcaGCGTGCCGTAGCGCGTGGCTTCTTTCGCCGTCGCATCGTTCTTGGTTTTCGCTTTAACACCGTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCAtcg                                                                                            <  2:265060/147‑3 (MQ=255)
                                                           tAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGt                                                                                           <  1:51965/150‑1 (MQ=255)
                                                             aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                         <  2:1031406/150‑1 (MQ=255)
                                                             aGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTa                                                                                         <  2:590695/150‑1 (MQ=255)
                                                               cGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTATCACCTTTGACCGCTTCGATGCCAGTAGCACCGGCATCGATGCCAATAGTGGCGTTTTCATGGAAGATCCAGTCATAAATGTATTCATAGTTAcc                                                                                       <  2:237656/150‑1 (MQ=255)
                                                               cGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTAcc                                                                                       <  1:1551205/150‑1 (MQ=255)
                                                                  gCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTTCCCAc                                                                                    >  1:1237573/1‑150 (MQ=255)
                                                                    cGTAGCGCGTGTCATCTTTCGCCTTCTCATCGTGCATGGTTTTCGCTTTAACACTTTTCACCGCTTCTATGCCCGTAGCAACGGCTTCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTg                                                                                  <  1:72275/150‑1 (MQ=255)
                                                                      tAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTa                                                                                <  1:1060038/150‑1 (MQ=255)
                                                                       aGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTAc                                                                               <  2:1479702/150‑1 (MQ=255)
                                                                         cgcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTg                                                                             >  1:555915/1‑150 (MQ=255)
                                                                          gcgTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGa                                                                            <  2:1248063/150‑1 (MQ=255)
                                                                               tCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAg                                                                       <  1:330848/150‑1 (MQ=255)
                                                                                 aTCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCCCTGTACTGATCCAgcg                                                                     >  1:901574/1‑150 (MQ=255)
                                                                                     ttCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGcacc                                                                 <  2:856341/150‑1 (MQ=255)
                                                                                        gCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACAGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTATTCAAGGAACATCCAGTCAAAAATGTAGACATAGTTACCCACTGTACTGATCCAGCGcaccaca                                                              >  2:1520234/1‑149 (MQ=255)
                                                                                           gTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAAc                                                           <  1:527798/150‑1 (MQ=255)
                                                                                            tctcATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACt                                                          <  2:1167765/150‑1 (MQ=255)
                                                                                                aTCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCg                                                      >  2:1546860/1‑150 (MQ=255)
                                                                                                 tCGTGCATGGTTTTCGCTTTAACACCATTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCgg                                                     <  2:672177/150‑1 (MQ=255)
                                                                                                  cGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCgtc                                                    >  2:917687/1‑148 (MQ=255)
                                                                                                  cGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGc                                                    <  2:469941/150‑1 (MQ=255)
                                                                                                   gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCCCTGTACTGATCCAGCGCACCACTAACACCCGGCg                                                   >  2:664085/1‑150 (MQ=255)
                                                                                                   gTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCg                                                   <  2:1090157/150‑1 (MQ=255)
                                                                                                     gCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGtt                                                 >  2:426898/1‑150 (MQ=255)
                                                                                                      cATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTc                                                >  1:774885/1‑150 (MQ=255)
                                                                                                          ggtttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATTGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTa                                            <  2:1563956/148‑1 (MQ=255)
                                                                                                          gTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACACACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTa                                            >  1:1432361/1‑150 (MQ=255)
                                                                                                             ttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTg                                         <  1:945538/150‑1 (MQ=255)
                                                                                                             ttCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTg                                         <  1:201951/150‑1 (MQ=255)
                                                                                                               cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc                                       <  2:561029/150‑1 (MQ=255)
                                                                                                               cGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGAc                                       >  1:1726622/1‑150 (MQ=255)
                                                                                                                gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGCCg                                      >  1:1124992/1‑150 (MQ=255)
                                                                                                                gCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACGGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACg                                      >  1:1130852/1‑150 (MQ=255)
                                                                                                                     aaCACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTggg                                 >  2:1502683/1‑150 (MQ=255)
                                                                                                                         ccTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTgg                             >  1:1097550/1‑150 (MQ=255)
                                                                                                                          cTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGc                            <  1:1049898/150‑1 (MQ=255)
                                                                                                                          cTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTGTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCCCCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGc                            >  1:112948/1‑150 (MQ=255)
                                                                                                                           tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                           <  1:1001952/150‑1 (MQ=255)
                                                                                                                           tttCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGcc                           <  1:1403920/150‑1 (MQ=255)
                                                                                                                              cACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCAt                        <  2:1553196/150‑1 (MQ=255)
                                                                                                                               aCCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCAGGGAAGAGCCAGTCAAAAATGTAGTCATACTTACCCCCTGTACTTATCCAGCGCACCACTAACTCCCGGCGTTCGGGACTGACGTTGGGCTGGCCCagt                       >  2:1172890/1‑148 (MQ=255)
                                                                                                                                ccGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCAttt                      <  2:1573407/150‑1 (MQ=255)
                                                                                                                                 ggcatCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCTTGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGGACTGACGTTGGGCTGGCCCATTTc                     >  1:1394499/5‑150 (MQ=255)
                                                                                                                                 cGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTc                     >  2:1020299/1‑150 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCCGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   >  1:1049638/3‑150 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                    >  2:192992/3‑149 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTcc                    <  1:192992/147‑1 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   >  1:1035844/3‑150 (MQ=255)
                                                                                                                                   catCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   >  1:1453812/3‑150 (MQ=255)
                                                                                                                                   cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   <  2:40344/150‑1 (MQ=255)
                                                                                                                                   cTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCt                   <  2:772315/150‑1 (MQ=255)
                                                                                                                                    atCGATGCCGGTGCTACGGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTg                  >  1:1329810/2‑150 (MQ=255)
                                                                                                                                    atCGATGCCGGTGCTACGGGCATCGATGCAAATAGTGACGTTATCATAGAAGATCAAGTAAAAAATGTATACATATTTAACCACTGTAATTATCAAAATAAAAACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTg                  >  2:203486/2‑150 (MQ=255)
                                                                                                                                     tCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGa                 <  1:1342534/150‑1 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt                >  2:532525/1‑150 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGAt                <  2:555915/150‑1 (MQ=255)
                                                                                                                                      cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATACAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTCGGCTGGCCCATTTCCTGaa                >  2:162529/1‑149 (MQ=255)
                                                                                                                                       gATGCCCGTGCTACGGGCATCGATGCCAATAGTGACGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCACACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg               >  2:234092/1‑150 (MQ=255)
                                                                                                                                       gATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATg               >  2:1673929/1‑150 (MQ=255)
                                                                                                                                       gATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCAAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGCCGTTGGGCTGGCCCAGTTCCTGATg               >  2:687096/1‑150 (MQ=255)
                                                                                                                                        aTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTTCCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGc              <  2:1237573/150‑1 (MQ=255)
                                                                                                                                        aTGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGc              <  2:1565250/150‑1 (MQ=255)
                                                                                                                                        aTGCCCGTAGCACCGGCATCGATGCCAATAGAGCCGTATTCAAGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCAATGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGc              <  1:887112/150‑1 (MQ=255)
                                                                                                                                          gCCCGTAGCAGCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGTTCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTTCTGACGTTGGGCTGGCCCATTTCCTGATGCtt            <  1:1262423/150‑1 (MQ=255)
                                                                                                                                          gCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCtt            >  2:1191950/1‑150 (MQ=255)
                                                                                                                                          gCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCtt            <  1:1255674/150‑1 (MQ=255)
                                                                                                                                           cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCATAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCt                               <  1:281013/130‑1 (MQ=255)
                                                                                                                                           cccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCt                               >  2:281013/1‑130 (MQ=255)
                                                                                                                                            ccGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTtat          <  2:928768/150‑1 (MQ=255)
                                                                                                                                              gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATAc        >  1:369572/1‑150 (MQ=255)
                                                                                                                                               tAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACt       <  1:626414/150‑1 (MQ=255)
                                                                                                                                                    ccGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTccggc  >  1:900065/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCGCCGTCTCATCGTGCATGGTTTTCGCTTTAACACCTTTCACCGCTTCGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGC  >  NC_000913/1449658‑1449955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: