Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,457,983 A→T 7.5% I163F (ATC→TTC)  paaF → 2,3‑dehydroadipyl‑CoA hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,457,9830AT 7.5% 224.2 / 6.1 79I163F (ATC→TTC) paaF2,3‑dehydroadipyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (39/33);  new base T (2/4);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCG  >  NC_000913/1457836‑1458132
                                                                                                                                                   |                                                                                                                                                      
tgCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCACCGTTTAATCCGTAGTGTCGGGATATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATc                                                                                                                                                      >  1:1453772/1‑150 (MQ=255)
        ggtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCTCTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTTATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                              >  2:1647520/1‑150 (MQ=255)
        ggtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTTATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCa                                                                                                                                              >  2:1148478/1‑150 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCag                                                                                                                                             <  2:1617956/150‑1 (MQ=255)
         gtggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCag                                                                                                                                             <  1:1588096/150‑1 (MQ=255)
          gggtTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCagc                                                                                                                                            <  1:114777/149‑1 (MQ=255)
           ggatGCCGGAGAGAATGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCAGGCCTGGCGCAGGCGGAACGCAACGGTAAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagca                                                                                                                                           <  2:690496/147‑1 (MQ=255)
            gtTGCCGGAGAGAACGCGCGTTTGGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGTGATACTTTCTCCGCTCagcac                                                                                                                                          <  1:1049567/150‑2 (MQ=255)
              tGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagcaagc                                                                                                                                        <  2:443596/150‑1 (MQ=255)
               gCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCACagcaagcc                                                                                                                                       >  2:216400/1‑149 (MQ=255)
               gCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTCGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCagcaagca                                                                                                                                       <  1:1534229/150‑1 (MQ=255)
                ccGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGcac                                                                                                                                      >  2:1070242/1‑150 (MQ=255)
                ccGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGcac                                                                                                                                      >  2:1075788/1‑150 (MQ=255)
                ccGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGcac                                                                                                                                      <  1:1143947/150‑1 (MQ=255)
                   gagagaACGCTCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGAAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcagc                                                                                                                                   <  2:1286905/150‑1 (MQ=255)
                     gagaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCAcagcag                                                                                                                                 >  1:799593/1‑150 (MQ=255)
                       gaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGc                                                                                                                               >  2:667567/1‑150 (MQ=255)
                       gaACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGGAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCCCAGCAGGc                                                                                                                               >  2:703668/1‑150 (MQ=255)
                          cgcgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATGATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCgg                                                                                                                            <  2:224718/150‑1 (MQ=255)
                            cgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACTGCTCAGCAAGCACAGCAGGCCGGGc                                                                                                                          >  2:1472378/1‑150 (MQ=255)
                            cgcgTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGc                                                                                                                          >  1:1326857/1‑150 (MQ=255)
                                    gggTTGCCGGAAATCACTCTCGGCATCATTCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACCGCAGGCCGGGCTGGTTAGc                                                                                                                  >  1:1358806/1‑150 (MQ=255)
                                     ggTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCg                                                                                                                 >  1:18405/1‑150 (MQ=255)
                                       ttGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGAc                                                                                                               <  2:566700/150‑1 (MQ=255)
                                        tGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACg                                                                                                              <  1:1306296/150‑1 (MQ=255)
                                         gCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGATCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGt                                                                                                             >  1:969378/1‑150 (MQ=255)
                                          ccGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTc                                                                                                            >  1:1643897/1‑150 (MQ=255)
                                           cGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCt                                                                                                           <  1:66537/150‑1 (MQ=255)
                                           cGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCt                                                                                                           >  1:1048734/1‑150 (MQ=255)
                                           cGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGATAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCt                                                                                                           <  1:143950/150‑1 (MQ=255)
                                                    ctctCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCg                                                                                                 >  1:1438465/1‑150 (MQ=255)
                                                      ctcGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAt                                                                                               >  2:849007/1‑150 (MQ=255)
                                                      ctcGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCAAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAt                                                                                               >  2:939480/1‑150 (MQ=255)
                                                        cGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAttt                                                                                             <  1:1173387/150‑1 (MQ=255)
                                                        cGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCAAGCGAttt                                                                                             >  1:868720/1‑150 (MQ=255)
                                                           catcatGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAAc                                                                                          <  1:417698/150‑1 (MQ=255)
                                                           catcatGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAAc                                                                                          <  1:1282646/150‑1 (MQ=255)
                                                            atcatGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAAcc                                                                                         <  1:477287/150‑1 (MQ=255)
                                                              catGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAAccca                                                                                       >  1:1664625/1‑149 (MQ=255)
                                                                 gCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGa                                                                                    >  1:1511724/1‑150 (MQ=255)
                                                                  ccTGGCTCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGaa                                                                                   <  2:877545/150‑1 (MQ=255)
                                                                  ccTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGaa                                                                                   <  1:1671075/150‑1 (MQ=255)
                                                                   cTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAAt                                                                                  <  2:943400/150‑1 (MQ=255)
                                                                     ggCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGAAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCAT‑CCCCAGCGATGTAACCCTCGAATAc                                                                                >  1:1338586/1‑150 (MQ=255)
                                                                        gcAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGcc                                                                             <  1:1088213/150‑1 (MQ=255)
                                                                          aGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCATGCCGGGCGGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCtt                                                                           <  1:311443/150‑1 (MQ=255)
                                                                                    cAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCa                                                                 >  2:968573/1‑150 (MQ=255)
                                                                                         tttAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAATTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGaa                                                            <  1:693476/150‑1 (MQ=255)
                                                                                           tAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGaaaa                                                          >  1:583837/1‑150 (MQ=255)
                                                                                             aTCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATg                                                        <  1:9412/150‑1 (MQ=255)
                                                                                               ccGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGc                                                      <  2:876676/150‑1 (MQ=255)
                                                                                                cGTATTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGAAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCa                                                     <  2:1536150/150‑1 (MQ=255)
                                                                                                cGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCa                                                     <  2:466857/150‑1 (MQ=255)
                                                                                                         ggTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTcg                                            >  1:583738/1‑150 (MQ=255)
                                                                                                          gTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGCCGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTcgc                                           >  1:938899/1‑150 (MQ=255)
                                                                                                           tacaTCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTcgcc                                          <  2:849750/147‑1 (MQ=255)
                                                                                                           tAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAAATAACCCACGAATACGCCTTACAGCTCGAATCGAAAATGTCACGTCACTcgcc                                          >  2:1597965/1‑150 (MQ=255)
                                                                                                                 gTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGc                                    >  1:1220345/1‑150 (MQ=255)
                                                                                                                 gTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGc                                    >  1:355056/1‑150 (MQ=255)
                                                                                                                  ttAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTTCCCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCttgt                                    <  1:1372369/150‑4 (MQ=255)
                                                                                                                    aGCCAGCATAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                                 <  2:474313/150‑1 (MQ=255)
                                                                                                                    aGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCtt                                 >  1:773745/1‑150 (MQ=255)
                                                                                                                     gCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGAGTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTa                                >  1:719669/1‑150 (MQ=255)
                                                                                                                              aTGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCa                       <  2:1053863/150‑1 (MQ=255)
                                                                                                                                ggTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCaaa                     <  1:939480/150‑1 (MQ=255)
                                                                                                                                 ttGCTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaag                    >  1:193036/2‑150 (MQ=255)
                                                                                                                                    cTGAGCGGTGATACTTTCTCCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaa                 >  1:822570/1‑150 (MQ=255)
                                                                                                                                     tGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaag                >  2:1334552/1‑150 (MQ=255)
                                                                                                                                      gAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaagc               <  2:220587/150‑1 (MQ=255)
                                                                                                                                      gAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAaagcaagc               <  1:1518723/150‑1 (MQ=255)
                                                                                                                                          ggAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCACGAATACGCCTTACAGCTGGCATCGAAAATGCCACGACACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTg           >  1:224304/1‑150 (MQ=255)
                                                                                                                                           gagaAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTgc          >  2:1051604/1‑150 (MQ=255)
                                                                                                                                            cgaAAGTCTCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTgcg         <  1:216400/149‑1 (MQ=255)
                                                                                                                                             gaAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCATTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCACGCGCTgcgc        >  2:1463449/1‑150 (MQ=255)
                                                                                                                                               aaGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCa      <  2:796218/150‑1 (MQ=255)
                                                                                                                                                 ttATGACCGCTCAGCAAGCACCGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGt    <  2:1453772/149‑1 (MQ=255)
                                                                                                                                                 gTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAgg    >  2:611661/1‑149 (MQ=255)
                                                                                                                                                  tATCACCGCTCAGCAAGCGCAGCAGGCCGGGCTGGTTGGCGCCGTCTT‑CGCCAGCGATTTAACCCTCGAATACGCCTTCCCGCTGGCATCGACAATGGCCCGGCACTCGCCGCTGGCGTTACAAGCGGCACAGCCAGCGCTGGGCCAGTc   >  2:1139836/1‑150 (MQ=255)
                                                                                                                                                  tATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTc   >  1:25396/1‑150 (MQ=255)
                                                                                                                                                   aTCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCg  <  2:1506305/150‑1 (MQ=255)
                                                                                                                                                   aTCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCGGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCg  >  1:32117/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
TGCGATGTGGTGGTTGCCGGAGAGAACGCGCGTTTTGGGTTGCCGGAAATCACTCTCGGCATCATGCCTGGCGCAGGCGGAACGCAACGTTTAATCCGTAGTGTCGGTAAATCGTTAGCCAGCAAAATGGTGCTGAGCGGAGAAAGTATCACCGCTCAGCAAGCACAGCAGGCCGGGCTGGTTAGCGACGTCTT‑CCCCAGCGATTTAACCCTCGAATACGCCTTACAGCTGGCATCGAAAATGGCACGTCACTCGCCGCTGGCCTTACAAGCGGCAAAGCAAGCGCTGCGCCAGTCG  >  NC_000913/1457836‑1458132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑