Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,462,028 G→A 7.5% G379D (GGT→GAT) ‡ paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,462,0280GA 7.5% 326.4 / 9.8 107G379D (GGT→GAT) ‡paaJ3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (46/52);  new base A (6/2);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 111 total aligned reads displayed.

GTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTT  >  NC_000913/1461882‑1462177
                                                                                                                                                  |                                                                                                                                                     
gTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCg                                                                                                                                                    <  2:1507019/150‑1 (MQ=255)
 ttGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGt                                                                                                                                                   <  2:1145597/150‑1 (MQ=255)
       gtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCa                                                                                                                                             <  2:1382058/150‑1 (MQ=255)
       gtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCa                                                                                                                                             >  1:1205487/1‑150 (MQ=255)
       gtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGACCGTTACGcc                                                                                                                                             <  2:1311468/150‑2 (MQ=255)
          ctacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATtg                                                                                                                                          <  2:1606575/150‑1 (MQ=255)
           tacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGACCGTTACGccgatg                                                                                                                                         <  2:545017/150‑6 (MQ=255)
             cgcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                       >  2:1471934/1‑150 (MQ=255)
             cgcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                       >  2:1484137/1‑150 (MQ=255)
             cgcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGc                                                                                                                                       >  2:551237/1‑150 (MQ=255)
              gcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCa                                                                                                                                      >  2:1704903/1‑150 (MQ=255)
              gcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCa                                                                                                                                      <  2:427114/150‑1 (MQ=255)
              gcgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCa                                                                                                                                      >  2:450163/1‑150 (MQ=255)
                gAATTGGGGATGCCTGATGATGCAGCACATCGTAACCCCACCGGAGGCGGTATCGCCTTAGGCCTTCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCCGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCAcc                                                                                                                                    <  1:102308/150‑1 (MQ=255)
                    tGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgt                                                                                                                                >  1:416643/1‑150 (MQ=255)
                     ggggCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgtg                                                                                                                               >  1:721810/1‑150 (MQ=255)
                         cTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGGGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGGCGTTACGCATTGTGCACCATGTGCATc                                                                                                                           <  2:1665173/150‑1 (MQ=255)
                           gCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCgg                                                                                                                         >  1:563910/1‑150 (MQ=255)
                           gCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCgg                                                                                                                         <  1:359129/150‑1 (MQ=255)
                              tgatgaTGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGgtgt                                                                                                                      >  2:383017/1‑150 (MQ=255)
                              tgatgaTGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGAGCCCGCCTGGCACTGGCTGCCAGCCATGAGCGGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGgtgt                                                                                                                      >  1:1178216/1‑150 (MQ=255)
                                atgatgCCCCACATGTTACCCCCAACGGAGGCGCTATCGCCTTAGGCCATCAGTTGGGAATGAGGGGGGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCg                                                                                                                    <  2:123330/150‑1 (MQ=255)
                                 tgatgCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCgg                                                                                                                   >  2:780869/1‑150 (MQ=255)
                                     gCCCCACATGTTTCCCCCACCGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGGTGCCAGCCATGAGCTGCAGCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCat                                                                                                               <  1:113113/150‑2 (MQ=255)
                                       cccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTc                                                                                                                 >  2:1182873/1‑146 (MQ=255)
                                       cccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTc                                                                                                                 <  1:1182873/146‑1 (MQ=255)
                                       cccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                             <  2:867397/150‑1 (MQ=255)
                                       cccACATGTTAACCCCAACGGAGACGCTATCGCCTTAGCCCAAACGTTGGGAATGAGTGGTGCCCGCCTGGCACTGCCTGACAGCCATTAGCAGAATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGAATATATTTCGGTCAgtg                                                                                                             >  2:304521/1‑148 (MQ=255)
                                        ccACATGTTATCCCCAAGGGAGGCGCTATCGCGTTAGGCCATCCGTTGGGAATGAGTGGTGCCGGCCGGGCACTGGCTGCCAGCCATGAGCTGCATAGGCGTTACGGTCGTTACGCTTTGTGCACCATGTGCATCGGTGGCTGTCTGGGc                                                                                                            <  2:1353542/150‑1 (MQ=255)
                                        ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGc                                                                                                            <  2:1667518/150‑1 (MQ=255)
                                        ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGc                                                                                                            >  2:1357365/1‑150 (MQ=255)
                                         cacaTGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCa                                                                                                           >  1:1499468/1‑150 (MQ=255)
                                              gTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGcc                                                                                                      <  1:1292794/150‑1 (MQ=255)
                                               ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                                     >  2:230426/1‑150 (MQ=255)
                                                 aaCCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCCTTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATg                                                                                                   >  1:656409/1‑150 (MQ=255)
                                                 aaCCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATg                                                                                                   >  2:710441/1‑150 (MQ=255)
                                                   ccccAACGGAGGCGCTATCGCCTTAGGCCCTCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCCGCCATGCGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCAGCGGTGTCGGTCCGGGCATCGCCATGag                                                                                                 >  1:1362609/1‑149 (MQ=255)
                                                   ccccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAt                                                                                                 >  2:561881/1‑150 (MQ=255)
                                                   ccccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAt                                                                                                 <  1:1301471/150‑1 (MQ=255)
                                                   ccccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCATGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAt                                                                                                 >  2:1510650/1‑150 (MQ=255)
                                                     ccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTc                                                                                               <  1:1194218/150‑1 (MQ=255)
                                                      cAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCt                                                                                              <  1:1336521/150‑1 (MQ=255)
                                                             ggCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgt                                                                                       >  2:1090176/1‑150 (MQ=255)
                                                               cgcTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCgtgt                                                                                     <  1:1357365/150‑1 (MQ=255)
                                                                  tATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCAGCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTg                                                                                  >  1:1111384/1‑150 (MQ=255)
                                                                    tCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGTGTGTTTGAg                                                                                <  2:558996/150‑1 (MQ=255)
                                                                              ccATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAcc                                                                      <  2:1440262/150‑1 (MQ=255)
                                                                              ccATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAcc                                                                      <  1:101206/150‑1 (MQ=255)
                                                                              ccATCCGGTGGGAATGAGTGGTGCCCGCCTGGCACTGGCGGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAcc                                                                      <  1:245709/150‑1 (MQ=255)
                                                                                 tCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                                   <  1:342297/150‑1 (MQ=255)
                                                                                 tCCGTTGGGAATGAGTGGTGCCCGACTGGCACTGGCTGACAGCCATGAGCTGAATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGc                                                                   <  1:1039800/150‑1 (MQ=255)
                                                                                    gTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                                >  1:1451520/1‑150 (MQ=255)
                                                                                        ggAATGAGTGGTGCCCGCCTGGCAGTGGCTGCCAGCAATGAGCTGCATCGGCGTAACGTTCGTTACGCATTGTGCACAATTTTCATCTGTGTCGTTCAGTGAATCGCCATGATTCTGTAGCGTGTTTGAGCATATCAACCTGCGAGTAcc                                                            <  2:63788/150‑1 (MQ=255)
                                                                                        ggAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTTACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCTTATCAACCTGCGAGTAcc                                                            >  1:559729/1‑150 (MQ=255)
                                                                                         gAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAccc                                                           <  1:839956/150‑1 (MQ=255)
                                                                                          aaTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCGCCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCt                                                          >  1:778827/1‑150 (MQ=255)
                                                                                           aTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTa                                                         <  1:710441/150‑1 (MQ=255)
                                                                                             gAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACa                                                       >  2:154079/1‑150 (MQ=255)
                                                                                                 ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGa                                                   >  2:117095/1‑150 (MQ=255)
                                                                                                  gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGAt                                                  <  1:1090176/150‑1 (MQ=255)
                                                                                                  gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGAt                                                  >  1:303712/1‑150 (MQ=255)
                                                                                                    gCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                                <  1:30741/150‑1 (MQ=255)
                                                                                                    gCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                                <  2:708042/150‑1 (MQ=255)
                                                                                                    gCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                                >  1:563310/1‑150 (MQ=255)
                                                                                                     cccGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGGCCTGGTTGACCATATACACCGGCCACTACCCAACAATGGTaaac                                              >  1:184267/1‑148 (MQ=255)
                                                                                                       cGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTAACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCa                                             <  2:198939/150‑1 (MQ=255)
                                                                                                        gCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCaa                                            >  1:652401/1‑150 (MQ=255)
                                                                                                         ccTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAAt                                           <  2:1703847/150‑1 (MQ=255)
                                                                                                         ccTGCCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAAt                                           <  2:284982/150‑1 (MQ=255)
                                                                                                          cTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATa                                          <  2:1111384/150‑1 (MQ=255)
                                                                                                           tGGCACTGGCTGCCATCCATGAGCTGCCTCGGCGTAACGGTCGTTATGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCAGCTCCAGGATTCTGGAGCGTGGTTGAGCATATCAACCTGCGAGTACCCTACAATGATCACCAATAc                                         <  1:885516/150‑1 (MQ=255)
                                                                                                           tGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGAATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATAc                                         <  2:1418284/150‑1 (MQ=255)
                                                                                                                   gCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTg                                 >  1:1107852/1‑150 (MQ=255)
                                                                                                                   gCTGCCAGCAATGAGCTTGATCGTCGTATAGGTCGTTAAGCATTGTGAACCATGTTCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCTTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTg                                 <  1:304521/150‑1 (MQ=255)
                                                                                                                      gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAcc                              >  1:978931/1‑150 (MQ=255)
                                                                                                                       ccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAccc                             <  2:1729439/150‑1 (MQ=255)
                                                                                                                       ccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAccc                             >  1:46928/1‑150 (MQ=255)
                                                                                                                           ccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt                         <  1:1103839/150‑1 (MQ=255)
                                                                                                                           ccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt                         >  2:24836/1‑150 (MQ=255)
                                                                                                                            caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                        <  2:721810/150‑1 (MQ=255)
                                                                                                                            caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                        >  2:102282/1‑150 (MQ=255)
                                                                                                                            caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                        <  2:1182251/150‑1 (MQ=255)
                                                                                                                               gAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGaaa                     <  2:1499468/150‑1 (MQ=255)
                                                                                                                                 gCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGCCCCGATTGAAAcc                   >  2:829821/1‑150 (MQ=255)
                                                                                                                                  cTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcg                  >  2:1142862/1‑150 (MQ=255)
                                                                                                                                  cTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcg                  >  2:1472232/1‑150 (MQ=255)
                                                                                                                                   tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgc                 <  1:877487/150‑1 (MQ=255)
                                                                                                                                      caatgCGTAACGACCGTTACGCCTTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTc              >  1:838989/5‑150 (MQ=255)
                                                                                                                                      aTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTc              >  2:1304318/1‑150 (MQ=255)
                                                                                                                                       aatgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATTACCAATACAAATCTTGACCCGATTGAAACCGCGTcg             >  1:824133/4‑149 (MQ=255)
                                                                                                                                       aatgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCt             >  1:1113788/4‑150 (MQ=255)
                                                                                                                                        cGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTg            >  1:782496/1‑150 (MQ=255)
                                                                                                                                        atgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTg            >  1:1695117/3‑150 (MQ=255)
                                                                                                                                        atgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTg            >  2:937001/3‑150 (MQ=255)
                                                                                                                                        atgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTg            >  2:943028/3‑150 (MQ=255)
                                                                                                                                            gTAACGGGCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtga        <  1:1526720/150‑1 (MQ=255)
                                                                                                                                              aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgatg      <  1:383017/150‑1 (MQ=255)
                                                                                                                                                  gTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGtt  <  2:652401/150‑1 (MQ=255)
                                                                                                                                                  gTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGAGCAGtt  >  1:338712/1‑150 (MQ=255)
                                                                                                                                                  gTCGTTACGCATTGTGCACCATGTGCATCGGTGTAGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGGATGACGAGATTGAAACCGCGTCTGTTGATGACtt  >  1:265827/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGTT  >  NC_000913/1461882‑1462177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: