Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,336 C→G 7.9% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,3360CG 7.9% 190.2 / 3.9 72pseudogene (945/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base C (35/30);  new base G (3/3);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTG  >  NC_000913/1466187‑1466484
                                                                                                                                                     |                                                                                                                                                    
aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTggcaccggcacc                                                                                                                                                      >  1:1273812/1‑150 (MQ=255)
  tGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCa                                                                                                                                                    <  1:712902/150‑1 (MQ=255)
   gATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAg                                                                                                                                                   <  1:1191796/150‑1 (MQ=255)
      atCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGAtt                                                                                                                                                <  1:892978/150‑1 (MQ=255)
         aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAt                                                                                                                                             <  2:1001650/150‑1 (MQ=255)
          ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGGGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                            <  2:515789/150‑1 (MQ=255)
          ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                            <  2:1155261/150‑1 (MQ=255)
              tGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtga                                                                                                                                        <  1:294147/150‑1 (MQ=255)
                     gcgACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGcc                                                                                                                                 <  2:1230553/150‑1 (MQ=255)
                           gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCa                                                                                                                           >  2:1033573/1‑150 (MQ=255)
                            tGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCaa                                                                                                                          >  2:414772/1‑150 (MQ=255)
                            tGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGCGCACCAACACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATCACTCCACAGCaa                                                                                                                          >  1:1093832/1‑150 (MQ=17)
                            tGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGCTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCCCCCAGAGTAATGGTTATGACGCCCCGGCaa                                                                                                                          >  2:923736/1‑150 (MQ=18)
                             ggAAAACAAGCCCACCATGACCGTCACCGAACCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCaaa                                                                                                                         <  1:412005/150‑1 (MQ=255)
                               aTAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                       >  2:1034807/1‑150 (MQ=255)
                                tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaa                                                                                                                      >  2:1387644/1‑150 (MQ=255)
                                  aCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGCAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaaca                                                                                                                    >  1:48497/1‑150 (MQ=255)
                                   cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGACGGAGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCACATTAAAGGTGATGACGCCCCGGCAaacaacaa                                                                                                                   >  2:1376174/1‑150 (MQ=17)
                                     aGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATGACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACg                                                                                                                 >  2:1715243/1‑150 (MQ=255)
                                     aGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCCCCAACGGTGGCACCGGCACCCAGATTAATAGTGGTGACGCCACGGCAAACAACAACg                                                                                                                 >  2:1322371/1‑150 (MQ=255)
                                      gggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                >  2:983417/1‑150 (MQ=255)
                                      gggCACCATGACCGTCACCGATCCGGAGGCCATGGGTATCCAGATCGACGGTGACAAGGCCCTCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGAGTAATGGTGATGACGCCACGGCAAACAACAACgg                                                                                                                <  2:668385/150‑1 (MQ=255)
                                            cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCCCCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACACCGGCAAAac                                                                                                          >  2:1666464/1‑150 (MQ=255)
                                            cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAac                                                                                                          >  2:1459195/1‑150 (MQ=255)
                                                    gcACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCTCGGCATACAACAACGGCAAAACCACCGTTg                                                                                                  <  1:3604/149‑1 (MQ=255)
                                                      aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                >  2:1134552/1‑150 (MQ=255)
                                                       ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                               <  1:1198812/150‑1 (MQ=255)
                                                       ccGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATAGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACg                                                                                               >  1:1521679/1‑150 (MQ=255)
                                                         gATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                                                             >  1:189552/1‑150 (MQ=255)
                                                               gAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAt                                                                                       <  1:1484412/150‑1 (MQ=255)
                                                                aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                      >  1:1599601/1‑150 (MQ=255)
                                                                aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGAtt                                                                                      <  2:848406/150‑1 (MQ=255)
                                                                   ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                            >  2:432784/1‑93 (MQ=255)
                                                                   ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                            <  1:432784/93‑1 (MQ=255)
                                                                   ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTccc                                                                                   >  1:1212532/1‑149 (MQ=255)
                                                                   ccATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCAACATCACCAACGGTTGCACAGGCAACCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGCCAAAACCACCGATGACTGCAAGGATTCCa                                                                                   >  1:776972/1‑150 (MQ=18)
                                                                       gggTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCgg                                                                               <  1:7791/150‑1 (MQ=255)
                                                                        ggTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGt                                                                              <  2:1273812/150‑1 (MQ=255)
                                                                            tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAg                                                                                                                                                   <  2:83058/77‑1 (MQ=255)
                                                                            tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACg                                                                                                                 >  1:303511/1‑111 (MQ=255)
                                                                            tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACg                                                                                                                 <  2:303511/111‑1 (MQ=255)
                                                                            tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAg                                                                                                                                                   >  1:83058/1‑77 (MQ=255)
                                                                            tCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACgg                                                                          >  1:1357845/1‑150 (MQ=255)
                                                                             ccAGATCGACGGTGACAAGGCCATCGTCAATAACGACGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGa                                                                         <  1:862351/150‑1 (MQ=255)
                                                                             ccAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGa                                                                         <  1:867858/150‑1 (MQ=255)
                                                                              cAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGCCGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                        >  1:139202/1‑150 (MQ=255)
                                                                              cAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                        <  1:133262/150‑1 (MQ=255)
                                                                               aGATCGACGGTGACTAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACACAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCTAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaaa                                                                       <  1:923736/150‑1 (MQ=255)
                                                                                gATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAt                                                                      >  1:1474122/1‑150 (MQ=255)
                                                                                       ggTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGt                                                               <  1:1134552/150‑1 (MQ=255)
                                                                                        gTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGta                                                              >  1:1209477/1‑150 (MQ=255)
                                                                                         tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                        <  2:823794/75‑1 (MQ=255)
                                                                                         tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                        >  1:823794/1‑75 (MQ=255)
                                                                                         tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCTCCGGTACGGAAATCAACg                                                                 <  1:425885/146‑1 (MQ=255)
                                                                                         tGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                 >  2:425885/1‑146 (MQ=255)
                                                                                            cAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGtaataa                                                          >  1:788334/1‑150 (MQ=255)
                                                                                              aGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGAAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACg                                                        >  2:537497/1‑150 (MQ=255)
                                                                                                   accGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCTCCGGTACGGAAATCAACGGTTATAACGGaaaa                                                   >  2:693734/3‑150 (MQ=255)
                                                                                                   aTCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGaaaa                                                   <  2:493710/150‑1 (MQ=255)
                                                                                                      gTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGtt                                                >  1:360984/1‑150 (MQ=255)
                                                                                                          aaaaCGAAGGCGAGAGCAACAACACAAACGCTGGCACCGGCACCCAGATTAATGGTGATGACGCAACGGGAAACAACAAAGGAAAAACAACCTTTGACGGCAAGGAATCCACCGCTACTTAAATCAACGGTAAGAACGGAAAAGTTAtac                                            >  2:1614470/3‑148 (MQ=255)
                                                                                                            aaCGAAGGCGAGAGCACCATCCCCAACGGTGGCACCGGCACCCAGATTAATGGTTATGCCGACACGGCAAACAACCACGGCAAAACCACCGTTGACGGAAAGGATTCCGCCGGAACGGAAATACTCAGTAATAACGGCAAAGTTATTccg                                          >  2:1077752/1‑148 (MQ=14)
                                                                                                            aaCGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAg                                          <  1:1387644/150‑1 (MQ=255)
                                                                                                                aaGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACg                                      <  1:1493121/150‑1 (MQ=255)
                                                                                                                  ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc                                    >  1:560476/1‑150 (MQ=255)
                                                                                                                     gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAGCAACGGGAATAACGGAAATGGTAGTCAGGGCGGCGAt                                 >  2:95804/1‑150 (MQ=17)
                                                                                                                      agagCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATc                                <  1:1715243/150‑1 (MQ=255)
                                                                                                                        agCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTg                              <  1:256189/150‑1 (MQ=255)
                                                                                                                          cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                            <  2:1474122/150‑1 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                 >  2:63924/1‑89 (MQ=38)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                 <  1:63924/89‑1 (MQ=38)
                                                                                                                                     aCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGCCGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcg                 >  1:1069444/1‑150 (MQ=255)
                                                                                                                                        gTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcggcg              <  1:414772/150‑1 (MQ=255)
                                                                                                                                             accggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc         >  2:1185918/1‑150 (MQ=255)
                                                                                                                                              ccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc                                                                            >  1:1207756/1‑82 (MQ=38)
                                                                                                                                              ccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTAc                                                                            <  2:1207756/82‑1 (MQ=38)
                                                                                                                                                    cccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg  <  2:1209477/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTG  >  NC_000913/1466187‑1466484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: