Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,504 A→G 5.4% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,5040AG 5.4% 216.8 / 2.7 74pseudogene (1113/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base A (28/41);  new base G (2/2);  total (31/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

ACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTG  >  NC_000913/1466358‑1466643
                                                                                                                                                  |                                                                                                                                           
aCGGCAAACAGCAACGGCAAAACCACCGTTGCCGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAATAGTTATTAAGGAAGGCGATATGGATGTCAGCGGCGGCGGGCACGGTATTGATATAACCGGCGACTGCGCaagg                                                                                                                                          >  2:271386/1‑148 (MQ=17)
  ggCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGt                                                                                                                                        >  2:728129/1‑150 (MQ=255)
   gCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTg                                                                                                                                       <  2:48497/150‑1 (MQ=255)
    cAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTgg                                                                                                                                      <  2:529399/150‑1 (MQ=255)
     aaaCAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGa                                                                                                                                     >  2:580178/1‑150 (MQ=255)
     aaaCAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGa                                                                                                                                     >  2:1304506/1‑150 (MQ=255)
          acaacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACTGAAATCAACGGTAATAACGGAAAGGTTATTCAGGACGGCGATGAGGATGTCAGCGGCGTCGGTCAAGATATTGATATCACCGGCGACAGCGCCACGGTGGATAACa                                                                                                                                >  2:118870/1‑150 (MQ=14)
           caacGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAAACGGTAATAACGGAAACGTGATTCAGGACGGCGATCTGGATGTAAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACaa                                                                                                                               >  1:1002562/1‑150 (MQ=255)
             acGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAgg                                                                                                                             <  1:1459195/150‑1 (MQ=255)
             acGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAgg                                                                                                                             <  1:1666464/150‑1 (MQ=255)
              cGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAggg                                                                                                                            <  1:983417/150‑1 (MQ=255)
                 cAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAc                                                                                                                         <  1:1034807/150‑1 (MQ=255)
                  aaaaCCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAcc                                                                                                                        <  1:712498/150‑1 (MQ=255)
                     accaccGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATg                                                                                                                     <  1:1322371/150‑1 (MQ=255)
                     accaccGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAGAACAAGGGCACCATg                                                                                                                     >  1:1597225/1‑150 (MQ=255)
                              gACGGCAAGGATTCCTCCGGTACGGAAATCAACGGTTATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCAcc                                                                                                            <  1:693734/150‑1 (MQ=255)
                              gACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCAcc                                                                                                            <  2:788334/150‑1 (MQ=255)
                                  gCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATc                                                                                                        >  2:571903/1‑150 (MQ=255)
                                   cAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATcc                                                                                                       >  1:1494757/1‑150 (MQ=255)
                                   cAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATcc                                                                                                       <  2:189552/150‑1 (MQ=255)
                                     aGGCTTCCACCGGTACAGAAATCAACGGTAATAACGGAAAAGCTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCgg                                                                                                     <  1:700550/150‑1 (MQ=255)
                                      ggATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                                                                                    >  2:1391784/1‑150 (MQ=40)
                                       gATTCCACCGGAACGGAAATCAACGGTAATAACGGAAAAGGTATTCAGGACGGCGATCTGGATGTCAGCGGCTGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                                   <  2:1521679/150‑1 (MQ=255)
                                           ccACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                                   >  2:330031/1‑146 (MQ=39)
                                           ccACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                                   <  1:330031/146‑1 (MQ=39)
                                           ccACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTcaa                                                                                               >  2:595736/1‑148 (MQ=39)
                                           ccACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAGAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCa                                                                                               >  2:1159505/1‑150 (MQ=255)
                                              ccGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCg                                                                                            <  2:1599601/150‑1 (MQ=40)
                                               cGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCgg                                                                                           >  1:934118/1‑150 (MQ=255)
                                                    cGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATcc                                                                                      >  2:479182/1‑150 (MQ=255)
                                                     ggAAATTTACGGTAATAAAGTAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGTATAACTAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCa                                                                                     <  1:595736/150‑1 (MQ=14)
                                                     ggAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATAACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCa                                                                                     >  1:1488316/1‑150 (MQ=255)
                                                         aTCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGCCAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGtt                                                                                 >  1:1245563/1‑150 (MQ=255)
                                                           cAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATATCAAGGTCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGa                                                                               >  2:870880/1‑150 (MQ=255)
                                                              cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAc                                                                                                                         >  2:330635/1‑105 (MQ=255)
                                                              cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCAc                                                                                                                         <  1:330635/105‑1 (MQ=255)
                                                              cGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGGCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACgg                                                                            <  2:139202/150‑1 (MQ=255)
                                                                gTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCg                                                                                                                 <  1:1624653/111‑1 (MQ=40)
                                                                gTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCg                                                                                                                 >  2:1624653/1‑111 (MQ=40)
                                                                gTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCg                                                                          >  2:1621530/1‑150 (MQ=25)
                                                                      aCGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAgg                                                                    >  1:1468227/1‑150 (MQ=39)
                                                                      aCGGAAAAGTTATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCTGTCACGGTATTGATTTCACCGGCGACAGCGCAACGGTGGATAACTAGGGCACCATGACCGGCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAgg                                                                    <  1:1614470/150‑1 (MQ=14)
                                                                       cGGAAATGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCTGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGc                                                                   <  2:1002562/150‑1 (MQ=12)
                                                                        ggAAAAGTTCTTCAGGACGGCGATCTGGAGGTCAGCGGCGGCGGGCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCg                                                                  <  1:1077752/150‑1 (MQ=255)
                                                                          aaaaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCg                                                                                            >  1:17721/1‑122 (MQ=35)
                                                                          aaaaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCg                                                                                            <  2:17721/122‑1 (MQ=35)
                                                                          aaaaGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgt                                                                >  2:553861/1‑150 (MQ=40)
                                                                           aacgTTATTCAGGAGGGCTATCTGGATGTCAGCGGCGGCGGTCACGGAATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgtt                                                               <  1:271386/147‑1 (MQ=255)
                                                                              gTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgtt                                                            <  1:1391784/150‑1 (MQ=40)
                                                                              gTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgttgtt                                                            <  1:95804/150‑1 (MQ=40)
                                                                               ttATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTa                                                           <  2:1357845/150‑1 (MQ=40)
                                                                               ttATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTa                                                           <  1:1304506/150‑1 (MQ=40)
                                                                                  ttCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaaca                                                        <  1:537497/150‑1 (MQ=40)
                                                                                  ttCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaaca                                                        >  2:203395/1‑150 (MQ=40)
                                                                                    cAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaac                                                      >  2:1262227/1‑150 (MQ=255)
                                                                                      ggACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGa                                                    <  2:1494757/150‑1 (MQ=255)
                                                                                       gACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGaa                                                   <  2:360984/150‑1 (MQ=255)
                                                                                                cTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAgc                                          <  1:728129/150‑1 (MQ=255)
                                                                                                   gATGTCAGCGGCGGCGGTCACGGTATTTATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGcc                                       <  2:1528898/150‑1 (MQ=255)
                                                                                                    aTGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCa                                      <  2:27076/150‑1 (MQ=255)
                                                                                                        cAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGCTTGACGGCGACCAGGCCGTTGTAAACAACGAAGGCGAGAGCCCCATCAc                                  >  1:1587927/1‑150 (MQ=255)
                                                                                                         aGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTTTCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGCGCGCCATCAcc                                 >  2:663251/1‑150 (MQ=255)
                                                                                                          gcggcggcgGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCa                                <  2:560476/150‑1 (MQ=255)
                                                                                                              cggcggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACgg                            <  1:1185918/150‑1 (MQ=255)
                                                                                                                 cggTCACGGTATTGATATCACCGGCGACAGCGCAACGGGGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAACGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTgg                         <  1:921861/150‑1 (MQ=255)
                                                                                                                  ggTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGc                        <  2:1069444/150‑1 (MQ=255)
                                                                                                                     cACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAcc                                                                       <  2:1454798/100‑1 (MQ=16)
                                                                                                                     cACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAcc                                                                       >  1:1454798/1‑100 (MQ=16)
                                                                                                                      aCGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCg                    <  1:580178/150‑1 (MQ=255)
                                                                                                                        ggTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCCCCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGc                  >  1:1505510/1‑150 (MQ=255)
                                                                                                                                tatCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGAt          <  1:553861/150‑1 (MQ=255)
                                                                                                                                tatCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGAt          >  1:747571/1‑150 (MQ=255)
                                                                                                                                  tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTa                                                           <  2:1444238/99‑1 (MQ=255)
                                                                                                                                  tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTa                                                           >  1:1444238/1‑99 (MQ=255)
                                                                                                                                        gCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtg  <  1:921046/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
ACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTG  >  NC_000913/1466358‑1466643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: