Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,471,095 G→C 6.9% pseudogene (579/3497 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,471,0950GC 6.9% 298.0 / 10.1 98pseudogene (579/3497 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (49/39);  new base C (2/5);  total (52/46)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 102 total aligned reads displayed.

TGCGAGATATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATC  >  NC_000913/1470946‑1471244
                                                                                                                                                     |                                                                                                                                                     
tGCGAGATATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATggcgg                                                                                                                                                       >  1:1270495/1‑150 (MQ=255)
 gcgcGATCTAGCTCGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATggcggc                                                                                                                                                      <  1:1240474/150‑1 (MQ=255)
  cGAGATATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTTGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGcc                                                                                                                                                     <  2:1231090/150‑1 (MQ=255)
  cGAGATATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGcc                                                                                                                                                     <  1:802728/150‑1 (MQ=255)
    agaTATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTa                                                                                                                                                   <  2:651454/150‑1 (MQ=255)
        ataGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGgc                                                                                                                                               >  2:522894/1‑150 (MQ=255)
         tagctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGgcg                                                                                                                                              <  2:1577382/150‑1 (MQ=255)
           gctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGcc                                                                                                                                            >  1:199653/1‑150 (MQ=255)
           gctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGcc                                                                                                                                            >  2:1459217/1‑150 (MQ=255)
             tagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAg                                                                                                                                          <  2:390046/150‑1 (MQ=255)
             tagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAg                                                                                                                                          <  1:942472/150‑1 (MQ=255)
             tagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGCGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTACAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCat                                                                                                                                          <  2:544049/150‑2 (MQ=255)
              agcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGc                                                                                                                                         <  2:4014/150‑1 (MQ=255)
              agcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCata                                                                                                                                         <  2:1387071/150‑3 (MQ=255)
              agcAACAGCGCCATTGACTTCGGTACAGGGGGGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCata                                                                                                                                         <  1:1616935/150‑3 (MQ=255)
                ccaCAGCGCCATTGACATCTGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCatacc                                                                                                                                       <  1:900831/148‑5 (MQ=255)
                cAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCaa                                                                                                                                       >  2:212005/1‑150 (MQ=255)
                 atcAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAAt                                                                                                                                      <  2:625112/148‑1 (MQ=255)
                 aaCAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCatacct                                                                                                                                      <  2:862526/150‑6 (MQ=255)
                   cAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATAc                                                                                                                                    >  2:669776/1‑150 (MQ=255)
                   cAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATAc                                                                                                                                    >  2:675886/1‑150 (MQ=255)
                    aGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACa                                                                                                                                   >  2:1596093/1‑150 (MQ=255)
                      cgcCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGt                                                                                                                                 <  2:1328531/150‑1 (MQ=255)
                       gcCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGtt                                                                                                                                <  1:996580/150‑1 (MQ=255)
                        ccATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGtta                                                                                                                               >  2:283540/1‑150 (MQ=255)
                               cTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATTGCGCCAGCAATACAGTTATTAACCa                                                                                                                        >  1:1591814/1‑150 (MQ=255)
                               cTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCa                                                                                                                        >  1:93294/1‑150 (MQ=255)
                                   ggTACCGGGGTGGGAGTTGGTACTGATAGTTATAGGGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAgggg                                                                                                                    <  2:1044593/150‑1 (MQ=255)
                                   ggTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAgggg                                                                                                                    >  2:1009721/1‑150 (MQ=255)
                                           tgtgGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCTGCCTATGGCGCCAGCAATACAGTTATTAACCATGGTACGATTaa                                                                                                            >  1:326453/1‑150 (MQ=255)
                                            gtgGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAAc                                                                                                           <  1:557899/150‑1 (MQ=255)
                                            gtgGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAAc                                                                                                           >  1:479144/1‑150 (MQ=255)
                                             ggggAGTTGGTACTGATAGGTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAAATGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTAAGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAAcc                                                                                                          <  2:56568/149‑1 (MQ=255)
                                              gggAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCt                                                                                                         >  1:1014175/1‑150 (MQ=255)
                                              gggAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCt                                                                                                         >  1:1266295/1‑150 (MQ=255)
                                              gggAGTTGGTACTGATAGTTATAGTGGTGAAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCt                                                                                                         <  1:110247/150‑1 (MQ=255)
                                               ggAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTc                                                                                                        >  2:733693/1‑150 (MQ=255)
                                               ggAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACAATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACATc                                                                                                        >  2:63906/1‑150 (MQ=255)
                                                taGTTGGTACTGATAGTTATACTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGGGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCg                                                                                                       <  2:1005511/149‑1 (MQ=255)
                                                    tGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGaaaa                                                                                                   >  2:648932/1‑150 (MQ=255)
                                                       tACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGaaaaaaa                                                                                                >  1:1526438/1‑150 (MQ=255)
                                                        aCTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGTGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAAt                                                                                               >  1:462126/1‑150 (MQ=255)
                                                        aCTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAAt                                                                                               <  1:313734/150‑1 (MQ=255)
                                                        aCTGATAGTTATAGTGGGGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCTGTGTTATAAGTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAAt                                                                                               <  1:125084/150‑1 (MQ=255)
                                                         cTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATg                                                                                              <  1:672360/150‑1 (MQ=255)
                                                         cTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATg                                                                                              <  1:492192/150‑1 (MQ=255)
                                                          tGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATgg                                                                                             <  1:1009721/150‑1 (MQ=255)
                                                           gATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGt                                                                                            >  1:729710/1‑150 (MQ=255)
                                                           gATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGt                                                                                            <  1:685936/150‑1 (MQ=255)
                                                               gTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACATTTTTTAACCAGGGGACGATTAAACTCGAAAAAAATGGTAAtt                                                                                        >  2:126769/1‑150 (MQ=255)
                                                               gTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAAtt                                                                                        >  2:836710/1‑150 (MQ=255)
                                                                    aGTGGTGCAGGGAAAAATGCAACAGCAATAAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTGTTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGAc                                                                                   <  1:368325/150‑1 (MQ=255)
                                                                       tgatcAGGGAAAAATGCAACAGCAATAAACCAATTGGTCTGTTTTATAACTATTTATAACGCCGGCGCAGGTATTGCGGCCTATGTCGCCATCAAAACAGTTATTAACCACGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGAt                                                                                <  1:1246412/146‑1 (MQ=255)
                                                                       ggtgCAGGGAAAAATGCAACAGCAATTAACCACTTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGTCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGAt                                                                                <  1:268386/150‑1 (MQ=255)
                                                                        gtgCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGATAAAAATGGTAATTATGACGATa                                                                               >  2:1295690/1‑150 (MQ=255)
                                                                          gCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGt                                                                             >  1:193382/1‑150 (MQ=255)
                                                                          gCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGt                                                                             >  2:618720/1‑150 (MQ=255)
                                                                            aGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTTTTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGCTAGTCt                                                                           >  1:1438276/1‑150 (MQ=255)
                                                                             gggAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTg                                                                          >  2:472787/1‑150 (MQ=255)
                                                                             gcgAAAAATGCAACAGCAATTAACAAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGGCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTg                                                                          <  1:884893/148‑1 (MQ=255)
                                                                               gAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgc                                                                        <  2:1686065/150‑1 (MQ=255)
                                                                                 aaaaTGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgcag                                                                      >  2:645841/1‑150 (MQ=255)
                                                                                  aaaTGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgcagc                                                                     >  1:927192/1‑150 (MQ=255)
                                                                                     tGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTACCCAGGGGACGATTAACCTCGAAAAAAATGGTAATTTTGACGATAGTCTGGCAGCaaa                                                                  >  2:1313750/1‑150 (MQ=255)
                                                                                            gCAATTACCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATTCAGTTATTAACCTGGGGACGATTACCCTCGAAAAAAATGGTAATTATTACGCTAGTCTGGCAGCAAATACTCTg                                                           >  2:1449691/1‑150 (MQ=255)
                                                                                            gCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTg                                                           >  1:357021/1‑150 (MQ=255)
                                                                                            gCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTg                                                           <  2:193382/150‑1 (MQ=255)
                                                                                             cAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTgg                                                          >  1:414023/1‑150 (MQ=255)
                                                                                               aTTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGTCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTa                                                        <  2:1321483/150‑1 (MQ=255)
                                                                                               aTTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTa                                                        >  1:705911/1‑150 (MQ=255)
                                                                                                ttAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAg                                                       <  1:522894/150‑1 (MQ=255)
                                                                                                 tAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAgg                                                      <  2:1207949/150‑1 (MQ=255)
                                                                                                  aaCCAATTGGGCGGTGTTATTACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAggg                                                     >  1:416368/1‑150 (MQ=255)
                                                                                                   aCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGa                                                    <  2:1689110/150‑1 (MQ=255)
                                                                                                    ccAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGAt                                                   <  1:1002395/150‑1 (MQ=255)
                                                                                                     cAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTTATTATGACGATAGTCTGGCAGCATATACACTGGTAGGGATg                                                  >  2:224234/1‑150 (MQ=255)
                                                                                                       aTTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGTCCTATGGCGCCAGCAATACAGTTATTAACAAGGTGACGATTAAACTCGAAAAAAATGGTAATTATGACGATATTCTGGAAGAAAATAATCTGGTAGGGATgga                                                >  1:31476/1‑149 (MQ=255)
                                                                                                       aTTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTAATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGc                                                <  1:1441322/150‑1 (MQ=255)
                                                                                                           gccGGCGTTATAAATAGTTATAACACCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGtt                                            >  1:1206369/3‑150 (MQ=255)
                                                                                                             cGGCGTTATAAATAGTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGtttt                                          >  1:636228/1‑149 (MQ=255)
                                                                                                              ggTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTAt                                         <  2:1477255/150‑1 (MQ=255)
                                                                                                               gtgtTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATg                                        <  2:62387/150‑1 (MQ=255)
                                                                                                               gggtTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATg                                        <  2:79436/148‑1 (MQ=255)
                                                                                                                 gtTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAg                                      >  2:158866/1‑150 (MQ=255)
                                                                                                                  ttATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGc                                     <  1:1596093/150‑1 (MQ=255)
                                                                                                                   tataACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCa                                    >  2:569889/1‑150 (MQ=255)
                                                                                                                    ataACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCAt                                   >  1:1706910/1‑150 (MQ=255)
                                                                                                                       aCTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGt                                >  1:1301881/1‑150 (MQ=255)
                                                                                                                         tATTTATAACGCCGTCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATTGTAc                              <  2:115243/150‑1 (MQ=255)
                                                                                                                               taACGCCGGCGCAGGTATGGCGGCATATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGTTAGGGATGGCTGTTTATGAGCATGGTACTGCTAt                        <  2:1534278/150‑1 (MQ=255)
                                                                                                                                aaCGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATc                       >  1:1723641/1‑150 (MQ=255)
                                                                                                                                      ggCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGAc                 >  1:1550847/1‑150 (MQ=255)
                                                                                                                                        cgcAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGAcaa               >  2:406538/1‑148 (MQ=255)
                                                                                                                                          tattgctGGCGCCATAGGCCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTAATGCTATCAACGACAAGa             >  2:219140/7‑150 (MQ=255)
                                                                                                                                           attgctGGCGCCATAGGCCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGAc            >  1:170963/6‑150 (MQ=255)
                                                                                                                                            ggTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACg           >  1:526493/1‑150 (MQ=255)
                                                                                                                                             gTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACgg          <  1:423617/150‑1 (MQ=255)
                                                                                                                                               aTGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGgt        >  2:585895/1‑150 (MQ=255)
                                                                                                                                                    ggcCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtat   <  1:1459217/150‑1 (MQ=255)
                                                                                                                                                     gcCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGATATCAACGAACAGACGGGTGTaatc  >  2:714783/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGAGATATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATC  >  NC_000913/1470946‑1471244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: